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Aiming at the disadvantages of thick seed coat and difficult cooking of red beans, a red bean product that can be co-cooked with white rice was developed through the pre-cooking technology of "microwave pretreatment-soaking-steaming". Based on the optimization of pre-cooking process by response surface methodology, the appearance, color, pasting properties, texture and re-cooking properties of pre-cooked red beans with different gelatinization degree were characterized. The results showed that the increase of microwave time, microwave power, soaking time and cooking time could significantly increase the gelatinization degree of red bean. Compared with raw red beans, the color of pre-cooked red beans became dark, and the volume expanded several times. The hardness and pasting viscosity of pre-cooked red beans also decreased, but their rehydration increased by 7.5-8.8 times. In addition, there was no white core after re-cooking under simulated cooking conditions of white rice. However, with the increase of gelatinization degree, the red bean granules swelled, and the epidermis seriously ruptured. The sensory score decreased, and the rehydration first increased but then decreased. In summary, the optimal pre-cooking process was determined as: microwave power 640 W, microwave time 30 s, soaking time 6.5 h, cooking time 20 min. Under these conditions, the gelatinization degree of pre-cooked red bean was 57.52%.

Objective: This study focuses on improving the comprehensive utilization rate of Polygonatum cyrtonema. Methods: The contents of total flavonoids, total saponins and total phenols from roots, stems, leaves, flowers and buds, of P. cyrtonema were determined. The scavenging rates of DPPH·, ·OH and nitrites in extracts of above tissue were measured and compared as well. Results: The contents of total flavonoids and total phenols five different kinds of non-medicinal tissue were in the same order, in which from highest to lowest was, leaves, buds, flowers, stems and roots, and the content of total saponins in flower was the highest In terms of antioxidant activity, the DPPH ·, ·OH and nitrite scavenging rate of leaf extract were the highest, followed by buds and flowers. Correlation analysis showed that the clearance of DPPH ·, · OH and nitrite by the extract from different tissue showed a certain positive correlation with the contents of total flavonoids, total saponins and total phenols. Conclusion: The extract of non-medicinal parts of P. cyrtonema has good biological activity.

BACKGROUND In recent years, copper isotopes have been widely applied in supergene environments and biogeochemical processes, acting as novel tracers for soil pollution and biogeochemical cycles during pedogenesis. To date, Cu isotope studies on natural soils have commonly analyzed basalt geostandards for monitoring data quality. However, the contents of copper, matrix ions, and organic matter in the soil and silicate rock are very different. For example, the copper content in silicate rock is >80μg/g, and the copper content in some soils is very low, that is, < 20μg/g. The use of silicate standard materials as reference samples to monitor the data quality of soil samples lacks representativeness. OBJECTIVES To provide new soil standards for high-precision Cu isotope analysis, this study reports high-precision copper isotope data for four soil reference materials (GBW07443, GBW07425, GBW07427, and GBW07389), expressed as δ65Cu relative to NIST SRM 976, which were measured using a multi-collector inductively coupled plasma-mass spectrometer (MC-ICP-MS). METHODS Soil samples were completely dissolved in high-pressure bombs in a muffle furnace. Complete separation of Cu from the matrices was obtained using a strong anion exchange resin (AG MP-1M). The instrument mass bias was corrected using the standard sample-standard method. RESULTS The long-term external reproducibility was higher than ±0.05‰ (n=306, 2SD). Cu isotopic compositions of the four soil reference materials, GBW07443, GBW07425, GBW07427, and GBW07389, from the China National Bureau of Standards were -0.04‰±0.04‰ (n=9, 2SD); -0.07‰±0.05‰ (n=12, 2SD); -0.06‰±0.04‰ (n=12, 2SD); and -0.02‰±0.06‰ (n=12, 2SD), respectively. CONCLUSIONS The δ65Cu values of these reference materials were close to zero and corresponded to the intermediate values of natural soils. Moreover, the sample was easy to obtain, and the experimental results showed uniformity in its chemical and copper isotopic compositions, making it suitable as a standard material for monitoring the reliability of the soil copper isotope chemistry and the mass spectrometry data.

Objective: This study aimed to classify the mechanism of pullulanase-catalyzed hydrolysis of waxy rice starch under electric field treatment, by monitoring the changes in hydrolysis efficiency, pullulanase activity, and the structure and properties of waxy rice starch. Methods: The hydrolysis efficiency was determined, and the structure and thermal properties of hydrolysates were characterized, by using scanning electron microscope, X-ray diffractometer, differential scanning calorimeterand rapid viscosity analyzer. Results: Under the control of the following conditions, electric field with intensities ≤2.5 V/cm could increase pullulanase activity, accelerate the hydrolysis, induce the pores and cracks on the granule surface, increase the relative crystallinity, increase the gelatinization temperature and reduce the pasting viscosity of waxy rice starch. However, electric field with intensities ≥5 V/cm could cause the partial inactivation of pullulanase and reduce the hydrolysis efficiency, and consequently caused less change of the relative crystallinity and pasting viscosity. Conclusion: The strengthening effect of the electric field treatment on pullulanase hydrolysis of waxy rice starch mainly affected pullulanase activity, but has no significant effect on the structure of starch.

Chitosan was mixed with carrageenan and tea polyphenols, and chitosan-carrageenan composite film was prepared by the fluidized bed method and its properties were tested.The light transmittance,mechanical property and water resistance of the composite film were investigated by single factor test, and then the response surface experiment was carried out to optimize the preparation process of the composite film with water vapor transmittance as the evaluation index.The results showed that the optimal process of chitosan - carrageenan edible composite film was as follows: adding 1.5% of chitosan, 0.4% of carrageenan,0.6% of glycerol, 0.6% of tea polyphenols, and drying temperature of 50 ℃.Under this condition, the thickness of the composite film was uniform and the mechanical properties were good.The water vapor transmission rate was 1.37 (10–11 g/m·s·Pa).

The structures of five kinds of millet from Shandong,Shanxi,Hebei and other provinces were studied by mid infrared (abbreviation:MIR) spectroscopy.The results showed that the infrared absorption modes of millet mainly include OH stretching vibration mode (νOH),CH3 asymmetrical stretching vibration mode (νasCH3),CH2 asymmetrical stretching vibration mode (νasCH2),CH2 symmetrical stretching vibration mode (νsCH2),CH stretching vibration mode (νCH),C==O stretching vibration mode (νC==O),protein amide Ⅰband mode (νamide-Ⅰ),protein amide Ⅱ band mode (νamide-Ⅱ),protein amide Ⅲ band mode (νamide-Ⅲ) and C—O stretching vibration mode (νC—O).Furthermore,the corresponding synchronous 2D-MIR spectroscopy of millet,including νasCH3-2d,νasCH2-2d,νsCH2-2d and νCH-2d,have some differences,which can quickly and accurately identify the five kinds of millets.This paper expanded the research scope of three-step MIR spectroscopy in structure and rapid identification of high-quality millet.

A series of composite photocatalysts were prepared by using graphene oxide (GO) and TiO2 hydrogel. The composite photocatalyst was characterized through SEM, XRD and Raman spectroscopy, the decolorization effect of pure TiO2 and composite photocatalyst on methylene blue (MB) dye wastewater under different experimental conditions was studied. The results show that TiO2 in composite photocatalyst is mainly anatase phase and its photocatalytic activity is better than pure TiO2. When the addition of GO reaches 15%(mass fraction), the photocatalytic activity is the highest. When 250mg composite photocatalyst is added to 30mL synthetic wastewater with a concentration of 10mg/L and an initial pH value of about 8, the decolorization rate can reach 93.1% after 2.5 hours, and the composite photocatalyst has visible photocatalytic activity. It is presumed that the photogenerated charges of GO/TiO2 composite photocatalyst may directly destroy the luminescent groups in the MB molecule,and no other new luminescent groups are generated during the treatment.

Timely and accurate acquisition of cropland spatial distribution data is of great significance for agricultural production management, planting structure adjustment and food security. In this study, three northeastern provinces (Heilongjiang, Liaoning and Jilin) were selected as the research area. Based on massive Sentinel-2 data, a 12-month Normalized Difference Vegetation Index (NDVI) dataset of cropland and non-cropland samples in a specific year was established in each province. The Google Earth Engine remote sensing computing platform was used to conduct supervised classification according to the difference characteristics of NDVI between cropland and non-cropland samples, and the spatial distribution data set of cropland resources with 10 m spatial resolution in the three northeastern provinces during 2020-2022 was obtained. The data set is an update of the latest available cropland resource data set, which can provide data support and scientific services for the scientific protection of phaeozem in Northeast China, the implementation of the strategy of "storing grain in land, storing grain in technology" and the guarantee of food security. [ABSTRACT FROM AUTHOR]

In this study, the advanced backcross population of semi-fertility plant 10 (sfpiO) mutation constructed by rice pollen semi-fertile mutant lsm and indica rice cultivar 93-11 (wild-type, WT) was used as the research subjects. Compared to the WT, there were no significant differences in plant height, leaf length, leaf width, tiller number, pollen number and other agronomic traits, but pollen fertility reduced significantly. Pollen microscopy and scanning electron microscopy (SEM) at the late stage of pollen development showed that starch accumulation in some pollen of sfp10 decreased observably, eventually led to pollen abortion. Physiological indexes related pollen development revealed that the contents of proline and starch in pollen of the sfp10 mutant decreased significantly. Sucrose accumulation generally increased in the upstream tissues (source leaf, sink leaf, and stem) of panicle, but sucrose content decreased significantly in panicle, indicating that transport from sucrose to panicle was affected. Genetic analysis indicated that the sfp10 phenotype was controlled by a pair of recessive nuclear genes. Gene preliminary mapping located the mutant site into a 398 kb interval between RM25389 and RM25404 on rice chromosome 10, which contained 3 genes related to sucrose transport and 1 gene related to starch synthesis. This study laid a foundation for further research on fine mapping, gene function, and regulation mechanism of pollen semi-fertility regulation genes. [ABSTRACT FROM AUTHOR]

A chitosan-phytic acid composite film with fresh-keeping performance was prepared using chitosan as film-forming agent and phytic acid as antioxidant with glycerol addition. The single factor experiments were carried out with the tensile strength, breaking elongation, water vapor transmittance and light transmittance of the composite films as the investigation indicators, then response surface test design with three -factor and three -level taking tensile strength as response value was conducted to optimize the chitosan, phytic acid, glycerol additions through Design Expert software. Finally the effects of composite films on fresh-keeping flavors of perches were explored taking sensory qualities, color differences and electronic nose analysis as indicators. The results showed that the optimal process formula of the composite membrane was determined as follows: chitosan addition of 1.5 g/100 mL, phytic acid addition of 0.06 g/100 mL, glycerin addition of 0.225%. The tensile strength of prepared film could reach to 29.281 MPa. The composite films wrapping could maintain high sensory qualities of perch fillets, and effectively control the decrease of L* and a* values, and the increase of b* value. The odor detection results by electronic nose demonstrated that the response intensity of perch meat wrapped in PE plastic film was greater than that in composite film group with the time extension. The chitosan -phytic acid composite film prepared under the optimal formula presented a certain fresh-keeping effects on the quality of perch fillets. [ABSTRACT FROM AUTHOR]

Objectives To analyze the evolution pattern of matrix (M) gene and amino acid site variation of M2 protein of influenza A (H1N1) pdm09 viruses in Hainnan province during the influenza epidemic season 2019-2020 (April 1, 2019 - March 29, 2020). Methods A total of 14 strains of influenza A (H1N1) pdm09 subtype virus was selected for sequencing. The phylogenetic trees were constructed by neighbor-jointing method, and the differences between the prevalent and vaccine strains in Hainan were compared by gene evolutionary trees. The sequencing results were analyzed by MEGA 10.1.8 and DNASTAR 7.0.1 software. Results During 2019-2020 influenza season influenza A (H1N1) pdm09 virus accounted for 4.9% of total influenza viruses detected in Hainan province. Phylogenetic analysis showed that M gene of influenza A (H1N1) pdm09 virus isolates in Hainan province from 2019 to 2020 could be divide into 6B.1 clade, be same with the international vaccine strain A/Brisbane/02/2018. The nucleotide and amino acid similarities were 98.7%-100.0%, 98.8%-100.0% respectively. And 12 isolates had mutations in amino acid S23N in the coding region of the extracellular region of the protein; all 14 isolates had mutations in the S31N site in the transmembrane region, and the mutation rate was 100.0%. There was 1 isolate with amino acid variation at the I39V locus and 9 isolates with amino acid variation at the E70D locus in the cytoplasmic region. Conclusions These data suggest that the activity of influenza A (H1N1) pdm09 virus was low level in Hainan province during 2019-2020 influenza season. The strains isolated in Hainan province have remained resistant to amantadine, and the circulating viruses were matched by current influenza vaccine. Special attention should be paid to the amino acid variation of M2 protein in future work to provide a scientific basis for the use of clinical anti-influenza virus drugs. [ABSTRACT FROM AUTHOR]

Objective To investigate the mechanism of the effect of melatonin (MT) on permeability of blood-brain barrier (BBB) in chronic cerebral hypoperfusion (CCH) rats. Methods The rat model of CCH was established by bilateral common carotid artery occlusion (BCCAO). A total of 72 male Sprague-Dawley rats were randomly divided into 4 groups: sham group (N = 18), BCCAO group (N = 18), the melatonin [5 mg/(kg·d)] treatment model group (MT1 group, N = 18), and the melatonin [10 mg/(kg·d)] treatment model group (MT2 group, N = 18). Evans blue staining and fluorescein- labeled glucoside (FITC- Dextran) staining marked by fluorescein isothiocyanate (FITC) were used to assess the permeability of BBB in rats. Expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 mRNA levels in basal ganglia of the brain were measured by quantitative real - time polymerase chain reaction (qRT - PCR). Expression of Occludin and Claudin - 5 protein in rat basal ganglia were measured by Western blotting method. Results Compared with the sham group, the fluorescence density in basal ganglia was increased (P = 0.000), expression of MMP-2 and MMP-9 were increased (all P = 0.000), and expression of Occludin and Claudin-5 were decreased (all P = 0.000) of BCCAO group. Compared with the BCCAO group, the fluorescence density in basal ganglia was decreased (P = 0.021), the expression of MMP-2 was decreased (P = 0.000) and the expression of Claudin-5 was increased (P = 0.000) in MT1 group, and the differences with sham group were statistically significant (P = 0.000, 0.006, 0.000). Compared with the BCCAO group, in MT2 group, the fluorescence degree in basal ganglia was further decreased (P = 0.000), the expression of MMP - 2 and MMP-9 were decreased (all P = 0.000), and were similar to the sham group (all P > 0.05), while expression of Occludin and Claudin-5 were increased (all P = 0.000) but lower than those of sham group (P = 0.003, 0.000). Compared with the two melatonin treatment groups, the efficacy of the 10 mg/(kg·d) group (MT2 group) was better than that of the 5 mg/(kg·d) group (MT1 group, all P < 0.05). Conclusions Melatonin may protect the integrity of BBB by inhibiting the degradation of Occludin and Claudin - 5 protein through inhibiting the expression of matrix metalloproteinases. [ABSTRACT FROM AUTHOR]

Objective To observe the protective function and mechanism of melatonin (MT) against white matter lesions in corpus callosum induced by chronic cerebral hypoperfusion (CCH) in cerebral small vessel disease (cSVD) rats. Methods Forty -four adult male Sprague - Dawley rats were divided into sham group, bilateral common carotid artery occlusion (BCCAO) group, MT1 group [BCCAO + melatonin 5 mg/(kg⋅d)] and MT2 group [BCCAO + melatonin 10 mg/(kg⋅d)]. Four weeks after operation, social activity (sniffing time and crossing number) was assessed to reflect apathetic behavior. Myelin basic protein (MBP) in corpus callosum was detected by immunofluorescence. Transmission electron microscope was used to compare the G-ratio. Western blotting test was used to detect phosphorylation level of mammalian target of rapamycin (mTOR). Results BCCAO group rats showed fewer sniffing time (t = 58.000, P = 0.000) and crossing number (t = 20.000, P = 0.000), lower level of MBP expression (t = 20.400, P = 0.000), higher level of G-ratio (t = - 9.800, P = 0.000), and lower pmTOR/mTOR ratio (t = 20.336, P = 0.000) than those of sham group; after treatment of melatonin [5 or 10 mg/(kg⋅d)], MT1 group and MT2 group showed more sniffing time (t = - 12.600, P = 0.001; t = - 26.000, P = 0.000) and crossing number (t = - 8.400, P = 0.000; t = - 10.200, P = 0.000), higher pmTOR/mTOR ratio (t = - 6.022, P = 0.014; t = - 8.800, P = 0.001) than those of BCCAO group but still lower than those of sham group (t = 45.400, P = 0.000; t = 32.000, P = 0.000; t = 11.600, P = 0.000; t = 9.800, P = 0.000; t = 14.314, P = 0.000; t = 11.536, P = 0.000); while showed higher level of MBP expression (t = - 16.800, P = 0.001; t = - 20.600, P = 0.000), and lower level of G-ratio (t = 8.600, P = 0.041; t = 9.200, P = 0.030) than those of BCCAO group but similar with sham group (P > 0.05, for all); MT2 group showed longer sniffing time than MT1 group (t = - 13.400, P = 0.000). Conclusions Melatonin could ameliorate apathetic behavioral and white matter lesions in corpu callosums in rats with cSVD, which might be related to the up-regulate of phosphorylation level of mTOR. [ABSTRACT FROM AUTHOR]

Soil samples, which were collected from three typical forests, i.e., Betula ermanii forest, coniferous mixed broad-leaved forest, and Pinus koraiensis forest, at different altitudes along the southern slope of Laotuding Mountain of Changbai Mountain range in Liaoning Province of China, were incubated over a temperature gradient in laboratory. Soil organic carbon mineralization rates (Cmin), soil β-1,4-glucosidase (βG) kinetics and their temperature sensitivity (Q10) were measured. The results showed that both altitude and temperature had significant effects on Cmin. Cmin increased with temperature and was highest in the B. ermanii forest. The temperature sensitivity of Cmin [Q10(Cmin)] ranked in order of B. ermanii forest > P. koraiensis forest > coniferous mixed broad-leaved forest, but did not differ significantly among the three forests. Both the maximum activity (Vmax) and the Michaelis constant (Km) of the βG responded positively to temperature for all the forests. The temperature sensitivity of Vmax [Q10(Vmax)] ranged from 1.78 to 1.90, and the temperature sensitivity of Km [Q10(Km)] ranged from 1.79 to 2.00. The Q10(Vmax)/Q10(Km) ratios were significantly greater in the B. ermanii soil than in the other two forest soils, suggesting that the βG kinetics-dependent impacts of the global warming or temperature increase on the decomposition of soil-organic carbon were temperature sensitive for the forests at the higher altitudes. [ABSTRACT FROM AUTHOR]

Objective To investigate the protective effects of recombinant human erythropoietin (rHuEPO) combined with alprostadil in treatment of acute kidney injury (AKI). Methods A total of 120 hospitalized patients with AKI (AKIN) who meet the diagnostic criteria and classification were enrolled in this study. The enrolled patients were randomly divided into 4 groups: control group, combination group, EPO group and Alprostadil group, with 30 cases in each group. Patients in the control group were only given routine hemodialysis treatment, patients in the EPO group were treated with rHuEPO subcutaneously injection of 3 000 U tiw for 14 days, patients in the Alprostadil group were treated with alprostadil intravenous infusion of 20 µg qd for 14 days, and patients in the combination group were given rHuEPO subcutaneously injection of 3 000 U tiw and alprostadil intravenous infusion of 20 µg qd for 14 days. The above four groups were observed for 28 days, the changes in urine volume in patients in each group were recorded, and the serum creatinine and urinary NGAL, IL-18 were detected on the 4th, 8th and 15th day. Results (l) The oliguria period in the combined treatment group was shorter than the control group (5 ± 0.8) days, vs (10 ± 2.3) days, with a significant difference (P < 0.05). The serum creatinine returned to normal time in the combined treatment group was shorter than the control group (10 ± 1.1) days, vs (16 ± 2.3) days, with a significant difference (P < 0.05). The urine NGAL and IL-18 had statistically significant difference between groups (P < 0.05). (2) Compared with the control group, the urine NGAL and IL-18 had statistically significant difference with the EPO group and Alprostadil group (P < 0.05), while the urine NGAL and IL-18 had no statistically significant difference between the EPO group an Alprostadil group. (3) The urine NGAL in combination group had statistically significant difference with the EPO group and Alprostadil group (P < 0.05), but the urine IL-18 had no statistically significant difference (P > 0.05). Conclusions (l) For acute kidney injury patients, rHuEPO combined with prostaglandin in treatment of acute kidney injury can shorten oliguric stage, spend less time on renal function recovery, which can be recommended in clinical. (2) On the basis of hemodialysis (HD), single use of EPO or Alprostadil has better effects than single use of HD in treatment of AKI. (3) Combination of EPO and Alprostadil has better effects than single use of EPO or Alprostadil in treatment of AKI. [ABSTRACT FROM AUTHOR]

Activated carbon was synthesized by using cheap corn stalk as carbon source and ZnCl2 as activator by microwave assisted. Then sulfur-carbon composite materials was synthesized with elemental sulfur by using melting method. The composite was analyzed by surface area and porosity analyzer XRD,FESEM for structure and morphology, and cyclic voltammetry (CV) and charge-discharge curve for electrochemical properties. Results showed that the activated carbon had specific surface area of 1 042. 2 m²/g and the total entrance of 0. 76 cm³/g. There were pores of different specifications distributed in internal acticated carbon and a large number of pores on activated carbon surface. The sulfur-carbon composite materials were tested when current density at 400 mA/g and scope of charge-discharge in 1.5 ~ 3 V, its initial specific discharge capacity was 608. 4 mAh/g, and capacity retention was 60% after 100 times, showing good electrochemical performance. [ABSTRACT FROM AUTHOR]

The dynamic model of cam mechanisms in high-speed loom is builded and simplified into a single-free-degree elastodynamics modeling. The dynamic design model of cam shedding mechanism is set up, with the aim of making the cam contour line smooth without impact, and then the design equation of the cam contour line is calculated, which is used to construct a simulation design for a case so as to form the curve the research needs. The polynomial curve obtained is smooth without impact and satisfies the need of dynamic features of cam in high-speed loom. The validity and the correctness of dynamic design model is proved by simulation results. [ABSTRACT FROM AUTHOR]

Taking thirty six wheat cultivars bred in different era and ecological regions as test materials, their seedlings growth under water stress was investigated, and their drought resistance was evaluated by gray correlation grade analysis. Significant difference was observed in the drought resistance among th e cultivars. The weighted drought resistance index ranged from 0.6580 to 0.2434. Among the test seventeen morphological and physiological traits, shoot dry mass had the greatest correlation degree (0.9473) with drought resistance, while SPAD had the smallest one (0.5356). The test cultivars were clustered into three groups, among which, eight cultivars belonged to strong drought resistance group, twenty three cultivars belonged to medium drought resistance group, and five cultivars belonged to drought sensitive group. The shoot dry mass, root dry mass, plant dry mass, plant height, root nitrogen accumulation, leaf area, and tiller number per plant differed significantly among the three groups, which could be used for evaluating the drought resistance of wheat cultivars at seedling stage. [ABSTRACT FROM AUTHOR]

This study aims to explore the mechanism of Shoutai Pills in treating threatened abortion. According to the random number table method, ICR female mice were randomized into a normal group, a model group, a dydrogesterone group, and a Shoutai Pills group, with 15 mice in each group. Mice were administrated with normal saline(normal and model groups) or the suspension of Shoutai Pills or dydrogesterone by gavage at 9:00 am every day. At 16:00 every day, mice in the normal group were administrated with an equal volume of distilled water, while those in the model, Shoutai Pills, and dydrogesterone groups were administrated with hydrocortisone solution by gavage for 4 consecutive days. ICR female and male mice were caged in a ratio of 2∶1 during the pre-estrous or estrous period. From the first day of pregnancy, drug administration was continued for 5 consecutive days. On day 6, mice were administrated with mifepristone by gavage to establish the model of kidney deficiency-induced abortion. On day 6 of pregnancy, 10 female ICR mice were randomly selected from each group, and the uterus was collected for observation of the pathological changes of trophoblasts at the maternal-fetal interface by hematoxylin-eosin(HE) staining. The protein levels of key enzymes of glycolysis, hexokinase 2(HK2), enolase 1(ENO1), pyruvate kinase M2(PKM2), and lactate dehydrogenase A(LDHA), were determined by Western blot and immunofluorescence. The expression of apoptosis-related proteins including B cell lymphoma-2(Bcl-2), Bcl-2-associated protein X(Bax), and cysteinyl aspartate-specific proteinase-3(caspase-3) was determined by Western blot and real-time PCR. Terminal-deoxynucleoitidyl transferase-mediated nick-end labeling was employed to examine apoptosis. The embryo loss rate of the remaining five female mice was calculated by trypan blue staining method on day 14 of pregnancy. On day 14 of pregnancy, the embryo loss rate of the normal group was 5.00%, which was lower than that(27.78%) in the model group(P&lt;0.05). Dydrogesterone and Shoutai Pills groups showed reduced embryo loss rates(10.26% and 7.50%, respectively) compared with the model group. On day 6 of pregnancy, compared with the normal group, the model group showed down-regulated expression of HK2, ENO1, PKM2, LDHA, and Bcl-2 and up-regulated expression of Bax and caspase-3(P&lt;0.05). Compared with the model group, dydrogesterone and Shoutai Pills up-regulated the expression of HK2, ENO1, PKM2, LDHA, and Bcl-2 and down-regulated the expression of Bax and caspase-3(P&lt;0.05). Compared with that in the normal group, the apoptosis rate in the model group increased(P&lt;0.05). Compared with the model group, dydrogesterone and Shoutai Pills reduced the apoptosis rate(P&lt;0.05). In conclusion, Shoutai Pills can reduce the embryo loss rate and protect embryos by promoting aerobic glycolysis at the maternal-fetal interface and inhibiting the apoptosis of trophoblasts in mice.

In order to explore the impact of optimized land use allocation on phosphorus loss in the runoff process of a small watershed in the Three Gorges Reservoir area, a traditional agricultural model catchment area (CG) and a catchment area after optimized land use allocation in the Shipanqiu watershed in Zhong County, Chongqing (EG) were selected as the research object, sampling at the outlets of the two catchment areas, respectively, to monitor the runoff process and different forms of phosphorus in rainfall events and to analyze the influence of land use configuration on the law of phosphorus loss. The results showed that:① in the 10 monitored rainfall and runoff processes, the ρ [total phosphorus (TP)] of EG was lower than that of CG, and the ranges of the two were 0.09-0.75 mg·L -1 and 0.13-2.82 mg·L -1 , respectively. Compared with that of CG, EG significantly reduced the peak value of TP. ② The average EMC of TP, total dissolved phosphorus (TDP), dissolved inorganic phosphorus (DIP), and particulate phosphorus (PP) in the process of rainfall and runoff was lower than that of CG, and EG and CG showed significant differences in EMC of TP, TDP, and DIP ( P <0.05); the main form of phosphorus loss in the two catchment areas in the process of rainfall and runoff was TDP, but the average TDP/TP of EG was larger. ③ The output load of EG was 45%, 43%, 57%, and 47% lower than that of the TP, TDP, DIP, and PP in CG. The output load of EG and CG of various forms of phosphorus was significantly correlated with the total runoff ( P <0.01). In addition, the slope of the linear fitting of various phosphorus forms in the CG catchment area to the total amount of runoff was 1.66 to 1.75 times that of EG. The output load of various phosphorus forms of CG was more sensitive to runoff than that of EG, and the output per unit area in the process of rainfall and runoff was more sensitive than that of EG. The amount of sand could have caused the difference in phosphorus concentration and output load more than the output per unit area. Optimizing land use configuration can effectively reduce the loss of various forms of phosphorus and provide a reference for the prevention and control of phosphorus loss in small watersheds in the Three Gorges Reservoir area.

Objective: To investigate the expressions of CD33 and CD13 in newly diagnosed multiple myeloma (MM) patients and its relationship with prognosis., Methods: It was retrospectively observed that the expression of CD33 and CD13 in 121 MM patients who were newly diagnosed from January 2014 to January 2020, and the relationship between the expressions of CD33 and CD13 and patients prognosis was analyzed., Results: Among the 121 newly diagnosed MM patients, there were 30 patients (24.8%) in the CD33 + group and 12 patients (9.9%) in the CD13 + group. Kaplan-Meier analysis showed that, compared with the CD33 - group, the progression-free survival (PFS) time and overall survival (OS) time were significantly shortened in MM patients in CD33 + group (PFS 17.5 vs 23 months, P=0.000; OS 18.5 vs 25 months, P=0.000); and the PFS time and OS time of MM patients in the CD13 + group were also significantly shortened than those in CD13 - group (PFS 21 vs 22 months, P=0.012; OS 25 vs 26 months, P=0.006). Cox regression analysis showed that CD33 and CD13 were independent adverse prognostic factors in MM patients (CD33: P=0.000;CD13: P=0.003)., Conclusion: CD33 and CD13 are prognostic risk factors in patients with MM.

Objective: To observe the changes of functional connectivity of brain pain-emotion regulation region in patients with cervical spondylosis of cervical type by functional magnetic resonance imaging (fMRI)., Methods: Thirty-two subjects were selected. Of them, 16 patients with cervical spondylosis of cervical type were divided into an observation group and 16 healthy subjects into a control group. The patients in the observation group were treated with acupuncture at Tianzhu (BL 10), Jingbailao (EX-HN 15), Jianzhongshu (SI 15) and ashi points for 30 min. The rest-state fMRI data was collected before and after acupuncture in the observation group. The subjects in the control group received no treatment, and the rest-state fMRI data was collected once. The visual analogue scale (VAS) score before and after treatment and the pain catastrophizing scale (PCS) score before treatment in the observation group were recorded. The resting-state brain functional imaging characteristics between the observation group and control group before treatment, between the observation group before and after treatment, were compared. Based on the brain functional connectivity of region of interest (ROI) the changes of functional connectivity in insula and ventral tegmental area (VTA) in emotional regulation brain region were observed, and the correlation between functional connectivity changes and VAS、PCS scores in patients of the observation group was analyzed., Results: In the observation group, the VAS score was (1.94±1.12) after the treatment, which was lower than (5.62±1.20) before treatment ( P <0.05). The PCS score before treatment was (19.18±8.42) in the observation group. Compared with the control group, the areas with increased functional connectivity with insula in the observation group before acupuncture included bilateral dorsolateral prefrontal lobe and right middle cingulate gyrus, and the areas with increased functional connectivity with VTA included right central posterior gyrus and right insula. In the observation group, the connectivity coefficient of left insula and left dorsolateral prefrontal lobe ( r =0.438, P <0.05), the connectivity coefficient of right insula and right dorsolateral prefrontal lobe ( r =0.483, P <0.05) were positively associated with the VAS score. In the observation group, the connectivity coefficient between the right insula and the right middle cingulate gyrus ( r =-0.560, P <0.05), the connectivity coefficient between the right VTA and the right insula ( r =-0.525, P <0.05) were negatively associated with the PCS score. After acupuncture, the areas with decreased functional connectivity with insula included bilateral posterior central gyrus, right anterior central gyrus, middle cingulate gyrus and left corpus callosum, while the bilateral suboccipital gyrus and left cerebellum showed increased functional connectivity with right insula. The areas with decreased functional connectivity with VTA included bilateral dorsomedial prefrontal cortex, left anterior cingulate gyrus, right middle temporal gyrus and left anterior cingulate gyrus. After acupuncture in the observation group, the functional connectivity of left VTA left dorsomedial prefrontal cortex and left anterior cingulate cortex ( r =-0.548, P <0.05), the functional connectivity of right VTA-bilateral dorsomedial prefrontal cortex and left anterior cingulate cortex ( r =-0.547, P <0.05) were negatively associated with the PCS score., Conclusion: Pain involves the formation and expression of "pain-emotion-cognition". Acupuncture can systematically regulate the brain functional connections between cognitive regions such as dorsal prefrontal lobe and anterior cingulate gyrus and emotional regions such as insula and VTA in patients with cervical spondylosis of cervical type, suggesting that acupuncture has a multi-dimensional and comprehensive regulation effect on pain.

To understand the effect of nitrogen from runoff during rainfall events for different land uses, sub-catchments A and B in the small Shipanqiu watershed in Zhong County, Chongqing-which were managed using different land use practices-were taken as research objects. Runoff flow and nitrogen levels at the outlet of the catchment were monitored. Sub-catchment A is an agroforestry-water complex and sub-catchment B is the site of traditional agriculture. EMC was used to evaluate the average concentration of runoff nitrogen during rainfall events, and the effect of this runoff nitrogen on the small watershed with different land use systems was analyzed. The results showed that the TN concentration in catchment B (1.37-15.17 mg·L -1 ) > catchment A (0.84-9.28 mg·L -1 ); the ratio of the first peak to the second peak in catchment A was 62%, which was far less than the 97% in catchment B; the average DN/TN values were 69% and 75% in catchments A and B, respectively; and the average NN/DN values were 67% and 80% in catchments A and B, respectively. The different land use practices have significant impacts on nitrogen loss. Compared with the catchment where traditional agricultural practices were followed, the agroforestry-water complex catchment effectively reduced the loss of nitrogen and decreased the first TN peak value and DN/TN and NN/DN values. This study provides a scientific basis for the prevention and control of non-point source pollution in small watersheds in the area of the Three Gorges Reservoir.

Objective: To study the developmental and morphological characteristics of the mandible in patients with impacted mandibular second molar and to predict the possible trend of mandibular development via three-dimensional (3D) measurement and analysis., Methods: A total of 88 cases of impacted group and 88 cases of control group were screened out. 3D measurements were performed by using Mimics software. A total of 23 landmark points and 17 measurements were determined. The measurements were analyzed by t-test., Results: The mandible length, the space between the first molars, the space between mandibular angles, and the width between the first molars in the impacted group were lower than those in the control group (P<0.05). Moreover, the value of the submandibular angle was higher than that in the control group (P<0.05)., Conclusions: The impacted mandible of patients with mandibular second molar showed lack of sagittal and width development, and the impacted mandibular second molar was a manifestation of its degeneration.

Objective: To investigate the function and mechanism of transcription factor of MEIS1 and miR-425 to the proliferation of chronic myeloid leukemia cell K562., Methods: Bioinformatic prediction was used to analyze the binding of MEIS1 in miR-425 promoter region. ChIP-qPCR coupled with dual luciferase assay was used to detect the combination of MEIS1 and the transcription activity of miR-425, and its regulative role in the transcription activity miR-425. CCK-8 was used to detect the effect of MEIS1 and miR-425 on cell proliferation. Flow cytometry with PI staining was used to detected the effect of MEIS1 and miR-425 on K562 cell cycle progression. Western blot was used to examine the effect of miR-452 on the expression level of MEIS1., Results: MEIS1 could bind the promoter of miR-425 and repressed its transcription. After K562 was transfected by shRNA, the K562 cell proliferation and cell cycle progression was significantly inhibitied. Moreover, after K562 cells were transfected by miR-425 mimic, cell proliferation and cell cycle was inhibited. The expression level of MEIS1 could be inhibited by the combination of miR-425 and MEIS1 3'UTR., Conclusion: MEIS1 can inhibit the activity of miR-425 in transcriptional level, while the miR-425 can suppress the expression of MEIS1 protein in post-transnational level. Therefore, a regulatory circuit comprising from MEIS1 and miR-425 regulates K562 cell proliferation.

Uptake of nitrogen (N) and phosphorus (P) and their purification capacity for five native submerged macrophytes were investigated in laboratory simulated hydrostatic conditions,including Hydrilla verticillata , Vallisneria natans , Ceratophyllum demersum , Myriophyllum spicatum , Potamogeton maackianus. The results indicated that the moisture contents of different submerged macrophytes were almost the same before and after the test,with the range of 89.8%-92.0%.The net accumulated biomass changed from 1.52 g·m -2 to 12.92 g·m -2 among different submerged macrophytes,and the highest net accumulated biomass of Hydrilla verticillata was 8.5 times higher than the lowest plant of Potamogeton maackianus .The N and P contents of five submerged macrophytes ranged from 26.54 to 34.44g·kg -1 and from 2.54 to 4.01g·kg -1 ,respectively,and the N and P contents of Ceratophyllum demersum were relatively high.Total N and P removal efficiency of different submerged macrophyte treatments had ranges of 63.8%-83.1% and 49.2%-70.8%,significantly higher than those of the CK treatment (39.9% and 36.9%),respectively,and the removal efficiency decreased in the order of Hydrilla verticillata > Ceratophyllum demersum > Vallisneria natans > Myriophyllum spicatum > Potamogeton maackianus .Total N and P removal efficiencies of different submerged macrophyte treatments were significantly correlated with net accumulated biomass,with correlation coefficients of 0.994( P <0.01) and 0.996( P <0.01).The contribution of direct N and P uptake to different submerged macrophytes had the ranges of 1.5%-13.3% and 2.2%-13.2%,and the synergism contribution (deducting self-purification capacity of water) of different submerged macrophytes ranged 22.5%-29.9% and 10.1%-20.6%,indicating that the synergistic effect of submerged macrophytes purification was much more significant than the direct uptake effect in the process of water purification.

3D in vitro toxicity testing model was developed by magnetic levitation method for culture of the human hepatoma cell line HepG2 and applied to evaluate the drug hepatotoxicity. After formation of stable 3D structure for HepG2 cells, their glycogen storage capacity under 2D and 3D culture conditions were detected by immunohistochemistry technology, and the mRNA expression levels of phase Ⅰ and Ⅱ drug metabolism enzymes, drug transporters, nuclear receptors and liver-specific marker albumin(ALB) were compared between 2D and 3D culture conditions by using RT-PCR method. Immunohistochemistry results showed that HepG2 cells had abundant glycogen storage capacity under 3D culture conditions, which was similar to human liver tissues. The mRNA expression levels of major drug metabolism enzymes, drug transporters, nuclear receptors and ALB in HepG2 cells under 3D culture conditions were up-regulated as compared with 2D culture conditions. For drug hepatotoxicity evaluation, the typical hepatotoxic drug acetaminophen(APAP), and most reported drugs Polygonum multiflorum Thunb.(Chinese name He-shou-wu) and Psoraleae corylifolia L.(Chinese name Bu-gu-zhi) were selected for single dose and repeated dose(7 d) exposure. In the repeated dose exposure test, 3D HepG2 cells showed higher sensitivity. This established 3D HepG2 cells model with magnetic levitation 3D culture techniques was more close to the human liver tissues both in morphology and functions, so it was a better 3D hepatotoxicity evaluation model., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)

Soil samples, which were collected from three typical forests, i.e., Betula ermanii forest, coniferous mixed broad-leaved forest, and Pinus koraiensis forest, at different altitudes along the southern slope of Laotuding Mountain of Changbai Mountain range in Liaoning Province of China, were incubated over a temperature gradient in laboratory. Soil organic carbon mineralization rates (Cmin), soil β-1,4-glucosidase (βG) kinetics and their temperature sensitivity (Q₁₀) were measured. The results showed that both altitude and temperature had significant effects on Cmin · Cmin increased with temperature and was highest in the B. ermanii forest. The temperature sensitivity of Cmin [Q₁₀(Cmin)] ranked in order of B. ermanii forest > P. koraiensis forest > coniferous mixed broad-leaved forest, but did not differ significantly among the three forests. Both the maximum activity (Vmax) and the Michaelis constant (Km) of the βG responded positively to temperature for all the forests. The temperature sensitivity of Vmax [Q₁₀(Vmax)] ranged from 1.78 to 1.90, and the temperature sensitivity of Km [Q₁₀(Km)] ranged from 1.79 to 2.00. The Q₁₀(Vmax)/Q10(Km) ratios were significantly greater in the B. ermanii soil than in the other two forest soils, suggesting that the βG kinetics-dependent impacts of the global warming or temperature increase on the decomposition of soil organic carbon were temperature sensitive for the forests at the higher altitudes.

The experiment is designed to explore pathological festures and material basis of pseadoanaphylactoid reaction induced by notoginseng total saponin preparation. Mouse pseadoanaphylactoid reaction was used, 50 ICR mice were randomly assigned to control group, positive medicine group, notoginseng total saponin preparation low-dose group, notoginseng total saponin preparation middle-dose group, notoginseng total saponin preparation high-dose group on average. They are treated by intravenous injection of test substance solutions containing 0.4% Evans blue (EB). 30 min later, scores of ear blue staining and quantitation of ear EB exudation were recorded. Another two experiment were repeated in the same way excluding EB, just to. detect the related cytokines in serum using ELISA. We found that the scores of pseudoanaphylactoid reaction in notoginseng total saponin preparation injection middle-dose group and high-dose group was evidently higher than that in control group, suggesting that notoginseng total saponin preparation injection may be can lead to pseadoanaphylactoid reaction. HE staining showed that pseadoanaphylactoid reaction induced by notoginseng total saponin preparation injection is related to inflammation. Histamine, VEGF and TNF-α levels in notoginseng total saponin preparation middle-dose group and high-dose group significantly increased (P < 0.05, P < 0.01) than control group and showed a dose-dependent manner as well as consistent with the degree of ear blue dye. While IL-6 and IL-10 content did not increase significantly in notoginseng total saponin preparation low-dose group and middle-dose group, but they significantly higher than control group (P < 0.05, P < 0.01) when it increased to quadrupe clinical concentrations, eight times of the clinical dose. So pseadoanaphylactoid reaction caused by notoginseng total saponin preparation may be related to histamine, VEGF, TNF-α, and it is possible that IL-6 and IL-10 can play a role when pseadoanaphylactoid reaction achieve a certain high degree.

Objective: To summarize the clinical characteristics of Burkitt's lymphoma., Methods: Clinical data of 41 Burkitt's lymphoma patients, treated from Jannuary 2009 to June 2014 in Chinese PLA General Hospital, were analyzed retrospectively., Results: Out of the 41 patients, 33 were males and 8 were females, with a median age of 13 (range, 1-67), 18 cases (43.9%) were in Ann Arbor stage I/II, and 23 cases (56.1%) were in stage III/IV. The commonest pathologicalal sites were head and neck (23 cases, 56.1%), and then the abdominal (41.5%), bone marrow (22.0%) and central nervous system (22.0%) could also be involved, while 7 cases (17.1%) were patients transformed into acute lymphocytic leukemia-type L3, 18% cases (3/16) were infected by EBV and 29.9% cases (6/38) were infected by HBV, 29 cases were treated with chemotherapy, their overal remission rate was 93.1(27/29 cases), 2-year overall survival rate(OS) was 83.3%(10/12 cases); 13 cases were treated with rituximab, their remission rate was 92.3%(12/13 cases), and 2-year OS was 66.7%(4/6 cases)., Conclusion: The 41 cases are more similar to the sporadic Burkitt's lymphoma, but the median age of its occurence is more younger, while the most common pathological sites are head and neck, and the short-term and high intensive chemotherapy with rituximab can obviously elevate remission rate for adult patients and prolong their survival time.

The t(8;21)(q22;q22) translocation is the most common chromosomal abnormalities in AML, and the chromosomal translocation forms AML1-ETO. The t(8;21) AML is a heterogeneity disease. It is unclear for how to treat the relapsed or refractory AML. Recently, the clinical trials and pathogenesis have made great progress. This article summarizes the current clinical trials and recruiting t(8;21) AML clinical trials and researches that related to treatment are as followed: epigenetics, JAK/STAT signaling, steroid, Chinese traditional medicine, and interferon. With the progress of pathogenesis researches, more and more treatments will translate into clinical trials, which can provide more optional choice for relapsed or refractory t(8;21) AML. In this article the AML1-ETO structure and t(8;21) AML pathogenesis, the clinical researches of t(8;21) AML treatment and basic researches of t(8;21) AML treatment are summarized.

Long non-coding RNAs (lncRNAs), which are longer than 200 nucleotides in length, widely exist in organisms and function in a variety of biological processes. Currently, most of lncRNAs found in plants are transcribed by RNA polymerase Ⅱ and mediate gene expression through multiple mechanisms, such as target mimicry, transcription interference, histone methylation and DNA methylation, and play important roles in flowering, male sterility, nutrition metabolism, biotic and abiotic stress and other biological processes as regulators in plants. In this review, we summarize the databases, prediction methods, and possible functions of plant lncRNAs discovered in recent years.

Objective: This study was aimed to analyze the expression and regulation mechanism of SIRT1 in AML1-ETO positive leukemia to find the core promoter., Methods: The real-time RT-PCR was used to detect the expression of SIRT1 in AML1-ETO positive leukemia cell line and clinical samples of leukemia patients, a SIRT1 promoter-luciferase reporter vector was constructed and the promoter activity was evaluated in the 293T cell line. A series of possible core promoter fragments of the SIRT1 5'-untranslated region were amplified by PCR, the PCR products were cloned into XhoI/HindIII-digested pGL3-Basic reporter vector, the poly-cationic compound SuperFect reporter vector complexes were transfected into 293T cells.The dual-luciferase Reporter Assay System was used to quantitate the reporter vector luciferase activity., Results: The six kinds of promoter fragment of SIRT1 gene were successfully constructed and cloned into the pGL3-Basic reporter vector, which was authenticated by XhoI/HindIII co-digestion and DNA sequencing. The luciferase activity of the promoter construct was significantly higher than that of the pGL3-Basic promoter in 293T cells. The luciferase report gene assay was also used to detect the regulation of AML1-ETO on the transcription activity of SIRT1 promoter. The results showed that the expression level of SIRT1 increased with the increase mens of AML1-ETO, the promoter of SIRT1 could be bound by AML1-ETO., Conclusion: The SIRT1 promoter-luciferase reporter vector is successfully constructed, the transfection system used in this study can effectively transfer gene in 293T cells. The SIRT1 core promoter possesses higher activity in 293T cells and can promote significantly expression of luciferase reporter gene in 293T cells. The transcription regulation of AML1-ETO on SIRT1 is carried out via promoting its promoter activity.

Objective: To investigate the protective effect and mechanism of Shenqi Compound on diabetic angiopathy modeled rats., Methods: Totally 18 SD rats were randomized into 3 groups, i.e., the normal control group, the diabetic mellitus (DM) group, and Shenqi Compound group, 6 in each group. The DM rat model was established by feeding high-fat diet (to induce hyperlipidemia) +intraperitoneal injection of small dose streptozotocin (STZ). Shenqi Compound was given to rats in the Shenqi Compound group at the daily dose of 2 g/kg. Equal volume of normal saline was given to rats in the model group and the normal control group by gastrogavage. All treatment was lasted for 12 weeks. Then 2-D and ultrasonic integrated backscatter technique were used to evaluate structural and functional changes of abdominal aorta in the progression of diabetic macroangiopathy. The fibrosis degree of the aorta vessel and myocardium capillaries were observed by using HE and Masson trichrome staining. The tension of the aortic vascular ring was determined. The transforming growth factor beta (TGF-beta) mRNA expression was detected by real time PCR (RT-PCR). The protein expression of TGF-beta, collagen I, collagen III, connective tissue growth factor (CTGF), and phosphorylation P38 MAPK were detected by Western blot., Results: Compared with the normal control group, abdominal aortic systolic inner diameter, diastolic inner diameter, Peterson elastic modulus, stiffness index, and backscatter integral significantly increased; the rangeability of integral backscatter and the extension coefficient of cross section significantly decreased in the DM group (all P < 0.05). After 12 weeks aforesaid indices were obviously improved in the Shenqi Compound group (P < 0.05). Results of HE and Masson staining showed that the fibrosis degree of the aorta vessel and myocardium capillaries was obviously alleviated in rats of the Shenqi Compound group (P < 0.05). Results of the aortic vascular ring tension showed that acetylcholine induced vasodilatation and maximum diastolic percent were obviously elevated in the Shenqi Compound group (P < 0.05). Compared with the normal control group, the mRNA expression of TGF-beta, and the protein expression of TGF-beta, collagen I, and collagen III, and phosphorylation of P38 MAPK all significantly increased in the DM group (P < 0.05). Compared with the DM group, the mRNA expression of TGF-beta, and the protein expression of TGF-beta, collagen I, and collagen III, and phosphorylation of P38 MAPK all decreased (P < 0.05)., Conclusions: Shenqi Compound could effectively improve the arterial function in diabetic marcoangiopathy and microvascular dysfunction. The mechanism might be due to the down-regulating the expression of TGF-beta, and further suppressing the phosphorylation of P38 MAPK, reducing the synthesis of collagen I and collagen III, therefore, ameliorating arterial and myocardial interstitial fibrosis.

This study was purposed to evaluate the outcome of acute myeloid leukemia patients treated with related peripheral blood hematopoietic stem cell transplantation (PBHSCT) and analyse the potential prognostic factors. A total of 64 acute myeloid leukemia patients treated with related peripheral allo-HSCT from march 2008 to august 2012 in our hospital were enrolled in the analysis. All the patients received either HLA-matched related or mismatched related donor mobilized peripheral blood stem cells. All the patients were followed up and evaluated for overall survival (OS), leukemia-free survival (LFS) and relapse rate (RR) , and the potential prognostic factors well analyzed. The results showed that the 3 year OS , LFS and RR were 61.9%, 52% and 39.1% respectively. Univarite analysis demonstrated that the disease status before transplantation (P < 0.01) , donor type (P < 0.01), white blood cell count at initial diagnosis (P < 0.05) are related with outcome, and severe aGVHD has some influence on the outcome (P > 0.05) . Multivariate analysis indicated the status of disease before transplantation, donor type, severe aGVHD are the most important prognostic factors. It is concluded that the related PBHSCT is effective treatment method for AML patients, recurrence is the main reason for the failure after transplantation, disease status before transplantation, donor type, and severe aGVHD are independent prognostic factors.

Objective: To explore the status of glucometabolic abnormalities in cardiological outpatients without previous diabetes diagnosis and with coronary artery disease (CAD) and hypertension., Methods: Patients without previous diagnosis of diabetes but with hypertension and CAD aged 18 years or above were recruited from cardiology departments of 11 general hospitals in China. Demographic data, disease diagnosis and medical history were collected. Physical examination and questionnaire survey were performed after the random blood glucose test. Oral glucose tolerance test (OGTT) examination was made for patients with fasting blood glucose ≥ 6.1 mmol/L or postprandial random glucose ≥ 7.8 mmol/L. Adjusted prevalence rates were used in the study., Results: A total of 7778 patients were included in 11 centers. After preliminary screening by fasting blood glucose and random blood glucose tests, 3861 patients were required to take OGTT, and 3019 (78.2%) patients actually took the test. 1287 out of 3019 patients screened with OGTT were diagnosed with diabetes, and the adjusted diabetes prevalence rate was 18.64% (1287/6904). The prevalence rate of diabetes was 19.87% (95/478), 9.22% (352/3819) and 14.81% (153/1033) in patients with CAD, hypertension, and CAD combined with hypertension, respectively. A total of 996 patients were diagnosed with impaired glucose tolerance (IGT) and its prevalence was 14.43% (996/6904). Of the enrolled population, 153 patients with random blood glucose lower than 7.8 mmol/L were also screened with OGTT test, 26.14% (40/153) patients met the diagnostic criteria of diabetes., Conclusion: A high prevalence of diabetes is found in patients without previous diagnosis of diabetes and with hypertension and CAD consulting at cardiology departments. We thus suggest to perform OGTT in this patient cohort to improve the early diagnosis of IGT and diabetes, and prevent missed detection of type 2 diabetes mellitus or IGT in cardiovascular patients with normal fasting blood glucose. Our results indicate that it was feasible to use OGTT as a screening tool for detecting diabetes in these patients and the patient compliance is satisfactory.

Objective: To explore the diagnostic and therapeutic methods for perioperative children with congenital heart disease (CHD) with airway stenosis in pediatric intensive care unit (PICU)., Method: Fiberoptic bronchoscopy was used for the diagnosis of 100 CHD cases in PICU who were clinically considered to have possible airway malformation because of complicated difficult-to-control lung infection, atelectasis and failure with the ventilator after surgery from January 2010 to October 2011. Cases who were confirmed to have severe airway stenosis by bronchoscopy and weaning from the ventilator after surgery were treated with balloon expandable stents into the desired position in the bronchoscopy., Result: There were 73 cases (73%) of CHD patients with airway abnormalities, including 31 cases of severe stenosis (31%), moderate stenosis in 29 cases (29%), mild stenosis in 13 cases (13%). Nine of the 10 children in whom the mechanical ventilation was hard to be stopped after surgery because of severe airway stenosis were weaned from mechanical ventilation successfully by fiberoptic bronchoscopy, while one case died from primary disease with severe sepsis after the placement of bronchial stents., Conclusion: CHD children with difficult-to-control lung infection, atelectasis and failure with ventilator after surgery are often complicated with airway abnormalities. The therapeutic bronchoscopy with airway stent can be used for cases with weaning from the ventilator because of severe airway stenosis.

Objective: To compare the difference in the efficacy on post-stroke constipation between acupuncture therapy of regulating qi circulation of fe-organ and Shengxue Tongbian Capsules., Methods: Seventy-five patients of post-stroke constipation were randomized into an acupuncture group (39 cases) and a Chinese medicine group (36 cases). The unit mode comprehensive therapy of stroke was adopted as basic treatment in the two groups. In the acupuncture group, acupuncture therapy of regulating qi circulation of fu-organ was added at Tianshu (ST 25), Zhigou (TE 6), Qihai (CV 6) and Zusanli (ST 36), once every day. In the Chinese medicine group, Shengrue Tongbian Capsules were supplemented for oral administration, once every day, 10 g each time. The clinical symptom score of constipation was observed before treatment, after 1 and 2 weeks treatment in the two groups, respectively. The efficacy in 1 week and 2 weeks of treatment and the adverse reaction were observed., Results: In 1 and 2 weeks of treatment, the clinical symptom score of constipation was reduced significantly as compared with that before treatment in the two groups (all P < 0.05). The improvements in the acupuncture group were significant than those in the Chinese medicine group in 2 weeks of treatment (8.03 +/- 2.38 vs 9.20 +/- 2.45, P < 0.05). Concerning to the occurrence of adverse reaction, there was 1 case of local bruises in needling local site in the acupuncture group; and there were 1 case of abdominal pain, 3 cases of diarrhea and 2 cases of nausea and vomiting in the Chinese medicine group., Conclusion: Both the acupuncture therapy of regulating qi circulation of fu-organ and Shengxue Tongbian Capsules achieve the significant efficacy on post-stroke constipation. The efficacy of the acupuncture therapy of regulating qi circulation of fe-organ is better and the adverse reaction is less after long-term persistent treatment.

The applications accepted and approved by general program, young scientist fund and fund for less developed region of national natural science funds in the discipline of Chinese materia medica, NSFC in 2012 have been introduced. The research contents of the funded projects in the popular research areas have been summarized and the problems in the applications have been analyzed to give a reference to the scientists in the field of Chinese materia medica.