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[Objective] To establish a simple, rapid and visual pathogen detection method for shrimp, and to achieve self detection by fishermen. [Method]The specific primers of LAMP (loop-mediated isothermal amplification technique) were designed for decapod iridescent virus 1 (DIV1). After optimizing the conditions of reagent dosage, temperature and reaction time, a visual and rapid detection method of LAMP combined with hydroxynaphthol blue (HNB) was established. [Result] 12 mmol/ L Mg2+ and 250 μmol/ L HNB solution in the LAMP reaction system was the most suitable condition at 65 ℃ for 60 min. The minimum detection limits of LAMP-HNB of DIV1 was 10² copies/ μL plasmid. The stability and specificity of the detection of DIV1 had also been verified using positive plasmids with target genes, and the results of 16 repeated experiments were relatively stable, multiple template amplification experiments did not show false positives, which meets the experimental requirements. [Conclusion] The LAMP-HNB visualization rapid detection method established in this study is suitable for the on-site rapid detection of shrimp DIV1 mentioned above. [ABSTRACT FROM AUTHOR]

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Changzhou University (Social Science Edition) is the property of Editorial Department of Journal of Changzhou University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aiming at the key issues such as poor user experience, large network overhead, and low access efficiency in the existing high performance computing user organization management system, a method for implementing a high performance computing user organization management system based on LAMP is proposed. This method adopts the B/S architecture and combines Twig and HTML, in order to reduce the burden on the server and improve the user experience. It uses the REST framework and the Cache mechanism to buffer massive temporary data, so as to reduce the development difficulty and network overhead. It uses trees structure to access the hierarchical data, which improves the data access efficiency and has good scalability. [ABSTRACT FROM AUTHOR]

The detection of genetically modified (GM) crops provides important technical support for the safety management of agricultural genetically modified organisms (GMOs). Loop-mediated isothermal amplification (LAMP) technology is easy to operate, efficient, sensitive, and specific. It has been widely used in the detection of GM crops. This article analyzes and summarizes the application of LAMP technology in the detection of GM soybean, maize and rapeseed in recent years, hoping to provide scientific references for further application and standardization of LAMP technology in the detection of GM crops. [ABSTRACT FROM AUTHOR]

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aiming at the problems of single green constraints and lack of engineering knowledge support in SMITH’s green modular design method based on atomic theory, an improved green modular design method was presented. The modules in the product were considered as atoms, and the components were mapped to nuclei with positive charge and electrons with negative charge. Thus, modules were divided by coulomb forces between components. The green constraint matrix including reuse, remanufacturing and material recovery constraints and calculation rules were designed to modify the coulomb force calculation formula. In addition, a modular merging method based on criteria was proposed to integrate the existing engineering knowledge into the modular merging process. Finally, taking the desk lamp as the object, the green modular design was carried out with the proposed method, and the improvement scheme was given. The results indicate that the number of modules obtained by the improved method is less than that of the original method. Furthermore,the introduction of multiple green constraints and the combination of modules based on criteria not only improve the greenness of products, but also improve the operability of the method. [ABSTRACT FROM AUTHOR]

The different localization and the different application scenarios between IOE and LAMP architecture were introduced. IT current situation and the driving force of removing IOE in telecom enterprise were analyzed. The differences of the business model，service characteristics and IT localization between the traditional telecom enterprises and the internet companies were studied. The overall implementation strategy of removing IOE and the suitable business scenario were proposed，and the effect of removing IOE from the billing system was expounded.

Objectives: To evaluate the application of loop mediated isothermal amplification (LAMP) technique for the detection of the deletion of outer membrane protein OprD2 gene in Pseudomonas Aeruginosa, it can provide reference for the early clinical diagnosis of imipenemresistant Pseudomonas Aeruginosa (IRPA) and the treatment plan. Methods: The IRPAof clinically isolated were collected from the Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine from January 2016 to December 2016, the MicroScan WalkAway-96 plus full automatic identification and drug sensitive system were used to detect the minimum inhibitory concentration (MIC), the deletion of OprD2 gene was detected by LAMP, and compared with real-time quantitative PCR (Real-time, PCR) method. Results: Among the 50 samples, both DNA A260/280≧1.72, both DNA concentration>370 ng/μL, and even the concentration of samples 6 and 49>2000 ng/μL. The OprD2 gene of 50 strains of IRPA was detected by LAMP, the results showed that 26 strains were positive and the other 24 were negative, and the deletion rate was 48%. The results of Real-time PCR showed that the positive and negative OprD2 genes of 50 strains of IRPA were 26 and 24 respectively, the result of LAMP method was consistent with that of Real-time PCR. Conclusions: LAMP method is a rapid, simple and convenient method for clinical laboratory application for the target of OprD2 gene design, and the detection result is highly consistent with the Real-time PCR method. [ABSTRACT FROM AUTHOR]

The challenge faced by IT of the traditional telecom under the environment of internet was introduced,IT construction experience from the excellent internet company was referenced,and the differences of the business model,service characteristics and IT localization between the traditional telecom enterprises and the internet companies were analyzed.IT target architecture of internet model and evolution strategy of the telecom enterprise were proposed,and the related efforts which were needed for the evolution were analyzed.

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao is the property of Chinese Society of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The article presents a study of the establishment of loop-mediated isothermal amplification (LAMP) detection method according to the Mycoplasma bovis P81 gene sequence and compared its sensitivity with nested polymerase chain reaction (PCR).

The article focuses on a study related to the application of loop mediated isothermal amplification method for the detection of Ornithobacterium rhinotracheale bacteria. Topics discussed include the use of incubation method at 63 degree celsius to amplify the bacterial DNA, comparison of the DNA amplification of the bacteria with other bacteria such as Salmonella pullorum, Escherichia coli, and Staphylococcusaureus, and comparison of incubation process with the polymerase chain reaction.

The article focuses on a study related to the detection of Tembusu virus E gene from ducks by using loop-mediated isothermal amplification (LAMP) assay. Topics discussed include development of the assay using primers which targets the E gene of the virus, determination of the cross-reactivity with other viruses such as duck plagues virus, avian influenza virus, and Newcastle disease virus, and the significance of the LAMP assay in the molecular epidemiological investigation of the virus.

In the panel display liquid crystal display, the use is extremely widespread, the amount of use is very big. But along with the liquid-crystal display massive applications, its main spare part-liquid crystal display monitor breakdown gradually is also paid attention by the people, this article main research liquid crystal display monitor essential spare part-back light source structure composition, and discusses its 5 kind of defects occurrence principle. [ABSTRACT FROM AUTHOR]

Gekko gecko (Tokay Gecko) is a valuable traditional Chinese medicine. In this study, the loop-mediated isothermal amplification (LAMP) technique was introduced for visual rapid identification of G. gecko from adulterants. A total of sixty-five 12S rRNA sequences of fourteen species of G. gecko and its adulterants were obtained. The results showed that G. gecko could be identified from its adulterants through BLAST analysis based on 12S rRNA regions. The 12S rRNA sequences of ten batches of G. gecko were conserved. There were only two haplotypes and three variation sites in the available regions for primers design. Six specific LAMP primers were successfully designed online based on 12S rRNA sequences. The visual rapid detection of G. gecko could be achieved with the optimized conditions (64 °C for 1 h and 80 °C for 5 min). And the required minimal template concentration was 5 μg·L⁻¹ while conventional PCR with 0.5 mg·L⁻¹. Consequently, the LAMP method established from this study was rapid, specific, highly sensitive, and simple. It could be applied to detect G. gecko from its adulterants efficiently., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)