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As a typical structural weak surface, bedding is ubiquitous in coal seams. The relative mechanical properties between coal seam bedding and coal matrix are the key to determining the effect of enhancing permeability by microwave in coal seams with bedding development. To this end, a self-developed microwave irradiation (MI) test device was used to carry out microwave fracturing coal experiments on coal samples with an angle of 0°, 30°, 45°, 60° and 90° between the bedding plane and the loading direction. The evolution characteristics of microscopic pore structure and microscopic cracks of coal samples with different bedding directions were explored, and the influence of bedding structure on the number, scale, and connectivity of coal pores and fractures under MI was revealed. The gas permeability characteristics of coal in different bedding directions before and after MI were compared and analyzed. The variation law of coal permeability under the influence of coal seam bedding and fracturing coal by microwave was clarified, and the influence mechanism of coal seam bedding on fracturing coal and enhancing permeability by microwave was revealed. The results show that the coal seam bedding has a significant effect on the effect of fracturing coal and enhancing permeability by microwave. The larger the angle between the bedding plane and the loading direction, the greater the reduction of the bound pores and the growth of the connected fractures of the coal under MI. Compared with the original coal samples, the order of magnitude difference between the permeability of coal samples in different bedding directions after MI is effectively reduced, and the permeability anisotropy is obviously weakened. The development of meso-cracks in coal under MI has a significant bedding effect. The specific evolution process is first to expand the original cracks along the bedding plane, followed by the initiation of new cracks along the bedding plane, and finally to expand the cracks intersecting with the bedding plane. The difference of dielectric loss and heat transfer properties between coal matrix and bedding plane makes the thermal stress distribution of coal subject to the bedding plane. When the bedding plane is perpendicular or parallel to the loading direction, the coal body is dominated by tensile failure. When the bedding plane is oblique to the loading direction, the coal body is dominated by shear slip failure.

With the rapid development of aquaculture in China in recent decades, the country now faces environmental problems, such as high energy consumption and severe environmental pollution. The Yangtze River Basin is the most important area for freshwater aquaculture production in China, accounting for more than half of the total production. The environmental issues induced by aquaculture have received widespread consideration in this area. However, the freshwater farming-induced environmental issues in the Yangtze River Basin were rarely evaluated in prior research. Life cycle assessment (LCA) is a valid tool for assessing environmental impacts and resources and is widely deployed in the industrial sector. It can quantify the impact of a product or service on different environmental indicators at various stages of the production process, thereby aiding in the identification of the best measures to reduce environmental impacts. The LCA is more effective than traditional environmental impact assessment methods; thus, it has been gradually applied in other fields, such as agriculture and aquaculture, in recent years. In aquaculture, LCA has been used to evaluate the environmental impact of various farming models or species in a laboratory setting. However, little attention has been paid to large-scale systems, such as the Yangtze River Basin. The two most important farming models in China are the pond farming model and the integrated rice-fish farming model. Among them, the integrated rice-fish farming model is regarded as environmentally friendly and an important step towards realizing the sustainable development of aquaculture in terms of the efficient utilization of water and land resources. Therefore, in this study, the environmental effects of pond farming and integrated rice-fish farming models were investigated in the Yangtze River Basin using the LCA method. A total of 20 monitoring points were established in the Yangtze River Basin, including 5 for integrated rice-fish farming and 15 for pond culture. In the integrated rice-fish farming model, the life cycle consists of two stages: Feed supply and breeding. In pond culture, the life cycle consists of three stages: Feed supply, power supply, and breeding. We analyzed the values of energy use (EU), global warming potential (GWP), acidification potential (AP), eutrophication potential (EP), and water use (WU) in the pond farming and integrated rice-fish farming models using the LCA method, assessed the contribution rate of each phase for environmental indices, and compared the environmental impact of the two farming models. In order to establish the inputs and outputs of an aquaculture system, experimental measurements and surveys were conducted to collect data. The collected data mainly included feed consumption, electricity consumption, water consumption, total phosphorus and total nitrogen emissions, and greenhouse gas (CH4 and N2O) emissions. The data were analyzed by the LCA software eBalance. In this study, the weight gain of 1 ton of aquaculture products served as the functional unit, and the results were standardized and evaluated in order to compare the environmental impacts of various impact indicators. The results showed that the standardized and weighted evaluation values of WU, EP, GWP, AP, and EU in the integrated rice-fish farming model were 11.650, 0.770, 0.141, 0.096, and 0.003, respectively, and the total environmental impact indicator was 12.660. In the pond farming model, the values of WU, EP, GWP, AP, and EU were 31.453, 1.187, 0.210, 0.174, and 0.007, respectively, and the total environmental impact indicator was 33.031. The environmental impacts of integrated rice-fish farming were lower than those of pond farming. The contribution rate analysis of the three stages of the life cycle to environmental impact showed that the contribution rates of EU, GWP, and AP in the feed supply stage were higher than those in the power supply and breeding stages. EP was primarily associated with the feed supply stage and pollutant discharge stages of the breeding process, and WU was principally concentrated on the breeding stage. Overall, the LCA results showed that the integrated rice-fish farming model had a more environmentally friendly effect than the pond farming model, which indicates that the Yangtze River Basin has considerable development potential. WU was the most influential environmental impact indicator and the main restriction factor in aquaculture; it was mainly associated with the breeding stage. Therefore, moderately increasing stocking density was an effective strategy for reducing water resource consumption and improving water resource utilization rates. In addition to WU, the main environmental impact index of the pond culture model and integrated rice-fish farming models comprised EP, followed by GWP, AP, and EU. The contribution rate analyses of the two models showed that the aquaculture-induced environmental impacts could be mitigated by improving feed production technology, establishing accurate feeding technologies, applying advanced effluent water treatment technologies, and appropriately increasing stocking density in the Yangtze River Basin. In summary, in this study, we compared the environmental impacts between the pond culture and integrated rice-fish farming models in the Yangtze River Basin using the LCA method. In addition, we analyzed the differences between environmental impacts at different production stages. These data served as a reference for the sustainable development and optimization of aquaculture in the Yangtze River Basin.

To solve basic ant colony algorithm’s drawbacks of large search space,low convergence rate and easiness of trapping in local optimal solution,an ant colony algorithm of partially optimal programming based on dynamic convex hull guidance was proposed.The improved algorithm dynamically controlled the urban selection range of the ants,which could reduce the search space of ants on basis of helping the algorithm to jump out of local optimal solution to global optimal solution.Meanwhile,the delayed drift factor and the convex hull constructed by the cities to be chosen were introduced to intervene the current ants’ urban choice,it could increase the diversity of the early solution of the algorithm and improve the ability of ants’ partially optimal programming.Then the pheromone updating was coordinated by using construction information of convex hull and the complete path information that combined local with whole,it could improve the accuracy of the algorithm by guiding the subsequent ants to partially optimal programming.The pheromone maximum and minimum limit strategy with convergence was designed to avoid the algorithm’s premature stagnation and accelerate the solving speed of the algorithm.Finally,the proposed algorithm was applied to four classic TSP models.Simulation results show that the algorithm has better optimal solution,higher convergence rate and better applicability.

Background and objective Lung cancer is one of the most important malignancies in China. Survival rates of lung cancer on the population-based cancer registry for the years 2001-2007 in Qidong were analysed in order to provide the basis for the prognosis assessment and the control of this cancer. Methods Total 4,451 registered lung cancer cases was followed up to December 31st, 2009. Death certificates only (DCO) cases were excluded, leaving 4,382 cases for survival analysis. Cumulative observed survival rate (OS) and relative survival rate (RS) were calculated using Hakulinen’s method performed by the SURV 3.01 software developed at the Finnish Cancer Registry. Results The 1-, 3-, and 5-year OS rates were 23.73%, 11.89%, 10.01%, and the RS rates were 24.86%, 13.69%, 12.73%, respectively. The 1-, 3-, and 5-year RS of males vs females were 23.70% vs 27.89%, 12.58% vs 16.53%, and 11.73% vs 15.21%, respectively, with statisitically significant differences (χ2=13.77, P=0.032). RS of age groups of 15-34, 35-44, 45-54, 55-64, 65-74 and 75+ were 35.46%, 17.66%, 11.97%, 13.49%, 10.61%, 15.14%, respectively. Remarkable improvement could be seen for the 5-year RS in this setting if compared with that for the years 1972-2000. Conclusion The lung cancer survival outcomes in Qidong have been improved gradually for the past decades. Further measures on the prevention, diagnosis and treatment of lung cancer should be taken.

Based on the hourly precipitation data from 2005 to 2021 at the 91 meteorological stations and Oceanic Niño Index in Guangxi in rain season, the spatial and temporal distribution characteristics of extreme short-time heavy rainfall are analyzed. The results show that: (1) In most parts of Guangxi, the intensity of extreme short-time heavy rainfall ranges from 40 to 70 mm·h-1. The highest intensity and frequency of extreme heavy rain are seen in the coastal areas, while the lowest intensity is seen in northern inland regions with more frequent heavy rain. (2) The number of extreme short-time heavy rainfall per year continues to rise. In May and June, the frequency of these events is the highest and the little monthly change of the heavy rainfall intensity has been observed. (3) The diurnal variation curve of the incidence of extreme heavy precipitation appears to be bimodal with peaks during 04∶00 BT-05∶00 BT and 05∶00 BT-06∶00 BT. The intensity of this kind of rainfall varies from 50 to 55 mm per hour with little variation in amplitude in different hours. (4) The spatial distribution characteristics of extreme short-time heavy rainfall is not only closely related to the terrain features, but also associated with the South China Sea summer monsoon onset and El Niño/La Nina event. (5) It is shown that there is a positive correlation between the Oceanic Niño Index of previous autumn and the frequency of extreme short-time heavy rainfall.

Calculation of design drainage modulus is of great importance for the safety of farmland and reduction of waterlogging loss. We investigated the effects of main meteorological factors on design farmland drainage modulus by focusing on a closed farmland area in Ninghe District of Tianjin City. To resolve the problem of limited observation, the parameter sensitivity was analyzed according to mutual information and partial rank correlation coefficient aiming at the total runoff depth based on field observation experiment. The reasonable parameters were obtained by combining with soil water content and the relative variation of groundwater level. Then, a three-source runoff yield model coupling of excess infiltration and saturation was established to estimate runoff and design drainage modulus, and the effects of meteorological factors such as precipitation and evaporation on drainage modulus were analyzed. Results showed that summer precipitation significantly reduced and evaporation varied a little during 1960-2015. The corresponding drainage modulus decreased with the reduction of precipitation. When return period increased, the magnitude of the reduction in precipitation as well as the design drainage modulus increased. However, the effects of meteorological factors on design drainage modulus was generally inapparent. Thus, we concluded that the current drainage modulus is suitable for the study area. [ABSTRACT FROM AUTHOR]

Venting is an essential operation step before starting the centrifugal pump. During the process of venting, the centrifugal pump faces the problems of oil splash, slop oil in the slop tank and excessive loss of crude oil during venting. Through the analysis on the actual production and operation of one oil transportation station in Xinjiang Tarim-Shihezi Pipeline, it is concluded that the main causes of the excessive crude oil loss during venting attribute to the excessively shallow and overlong guide oil tank for draining, and the air bubbles in the vent pipeline. Based on the investigation on venting of centrifugal pumps before pump starting in China and abroad, the following improvement measures were proposed according to the starting steps, precautions and the actual situation of the oil transportation station. The additional three-way funnel can be used to cope with the crude oil loss caused by the excessively shallow and overlong tank. The vent pipeline can be moved from the side of the centrifugal pump to the rear (the moving distance of about 47 cm) and connected with the three-way funnel to avoid crude oil splashing caused by bubbles in the vent pipeline. The practice shows that this method can effectively reduce the loss of crude oil during venting before starting the centrifugal pump, and the single loss of crude oil during venting declines from 79 g to 0. The improvement measures are simple and safe for operation and capable of meeting the actual needs of the site, so they are supposed to provide important practical significance for optimizing and improving the overall performance of the centrifugal pump.

Line spectra in the radiated noise of marine vessels are the most visible signals, which can be detected, tracked and identified by passive sonar of the enemy, and hence it is of great significance to reduce the line spectra for improving the acoustic stealth of marine vessels. The technology of chaos spectra reduction was used to reduce line spectra in the radiated noise of submarines based on the fact that response spectrum of a nonlinear system under harmonic excitation is a continuous one when chaos occurs. The feedback chaotification of the single-degree-of-freedom vibration system and multi-degree-of- freedom vibration systems subjected to harmonic excitation were analyzed, and the chaotification method of the multi-degree- of-freedom vibration system subjected to multi-frequency excitation was proposed. The study discovered the condition and approach that the vibration system enters the chaos state subjected controller effect, and overcome the difficulties that how to maintain the chaotic motion when the operation condition and the environment change. The experimental platform of the two- degree-of-freedom VIS for discretization and chaotification was designed, and the dynamics of the system with different feedback plus was experimentally studied. The results confirmed the effectiveness of this method, and can provide the theoretical and experimental bases of design for chaos VIS. [ABSTRACT FROM AUTHOR]

The article discusses the result of the study which shows that Eperythrozoonisis in dairy cow could be transmitted through respiratory system.

An equal scale test was conducted for the bottom curtain construction method of curved pipe jacking of “Yangtze River Estuary II”, which included lowering the end plate and advancing the pipe joint. The test results show that the curved pipe jacking bottom curtain method is suitable for the integral salvage of sunken ships. The method of using a stirring pump to remove iron plate sand on the end plate is inefficient, which should be replaced with a more efficient method in the future. The maximum thrust for propulsion in the three pipe joints is 2.2 MN, and the maximum torque is 28 kN·m.

Pseudomonas, an important spoilage bacterium in animal-derived foods as well as fruits and vegetables in the cold chain, can cause huge economic losses to the food industry. The food spoilage ability of Pseudomonas is closely related to the formation of biofilm, which can enhance the survival and adaptability to the food processing and storage environment of Pseudomonas, causing persistent contamination. However, a systematic review of the biofilm formation of Pseudomonas and its mechanism is still lacking at present. This article describes the contamination status of Pseudomonas in fresh foods, analyzes the biofilm formation and extracellular matrix composition of Pseudomonas, and elucidates the major regulatory factors of the biofilm formation of Pseudomonas such as cyclic diguanylate, quorum sensing system and small RNAs. We believe that this review will provide a theoretical reference for uncovering the mechanism of food spoilage caused by Pseudomona and controlling its biofilm contamination.

About 40%-70% of newly diagnosed prostate cancer patients in China are in the stage of metastatic disease, and the spatio-temporal heterogeneity of the occurrence and development of prostate cancer and the unique metastasis pattern make it difficult to analyze immune markers based on biopsy tissue. With the advancement of many basic and clinical studies, new advances have been made in the pathogenesis, diagnostic methods, perioperative management, radiotherapy techniques and systematic treatment of advanced diseases of prostate cancer. These results continuously enrich the means of diagnosis and treatment of prostate cancer patients and improve their prognosis. Here was a review of the major advances in prostate cancer research in 2022.

During the pandemic of COVID-19, in order to respond to the call of the Ministry of Education that “teaching and learning are not stopped even though courses are stopped”, universities across the country have launched online teaching. Taking the “functional experiment” course in Beijing University of Traditional Chinese Medicine as an example, this course was carried out using online virtual experiment platform. On the basis of experimental teaching during the pandemic, teachers have collected experience and students' feedback. This paper summarizes the strengthens and weakness of this teaching method during the pandemic.

For the minimum power broadcast scheduling problem where network uses sleep scheduling and each node's transmission power is continuously adjustable,a recursive approach to compute the optimal transmission scheduling of a node was firstly presented,and then a discrete particle swarm optimization algorithm to construct the minimum power broadcast scheduling was proposed.This algorithm searches for the optimal broadcast arborescence,and utilizes the greedy algorithm based on the minimization of the broadcast's power increment and the local optimization algorithm based on the heuristic adjustment of the broadcast arborescence to improve the convergence speed and the result quality.The simulation results show that the proposed algorithm is able to effectively reduce the broadcast power.

In wireless ad hoc networks,because devices are powered by batteries,and multicast applications are constantly emerging,how to construct a multicast tree of the minimum energy consumption is an important problem.For the effect of the different choices of relay nodes to the construction of the minimum energy consumption multicast tree,a discrete particle swarm optimization algorithm to optimize the construction of the minimum energy consumption multicast tree was proposed.In order to avoid the premature convergence of the discrete particle swarm optimization algorithm,an inertia weight strategy was introduced to balance the global searchin ability and the local searching ability.The results of simulated experiments show that the modified discrete particle swarm optimization algorithm has strong optimization ability,and can effectively optimize the construction of the minimum energy consumption multicast tree.

Abstract: Based on the data of regional geologic background, core, and thin section, this paper studies the characteristics of sedimentary facies and reservoirs of the Upper Cambrian-Ordovician in Well Gucheng-4, Tarim Basin, and points out the potential and targets of petroleum exploration in the Gucheng break slope. Collapsed breccias are deposited in platform margins of the Upper Cambrian; Ordovician Penglaiba, Yingshan, and Yijianfang Formations sediments are controlled by eophyton, and lime mud mound facies of shallow glacis of platform frontal margin; Strumae limestone is deposited in the Ordovician Tumuxiuke Formation; and slope continental shelf abyssal basin facies controll the sediments in the Ordovician Queerqueke Formation. The Upper Cambrian dolomite is a better reservoir, which is controlled by deposition environment, dolomitization, and structural fractures. The Ordovician limestone reservoir is poor because of most primary pores being filled by cementation. The favorable exploration targets may lie in the north and west of the Well Gucheng-4 area. Key words: Tarim Basin, Well Gucheng-4, break slope, sedimentary facies, reservoir characteristics

For status that most coal mine safety monitoring and control systems adopt fixed and wired sensor network which has blind spots, the paper proposed a design scheme of mine environmental data acquisition equipment based on wireless ad hoc networks, and introduced design of low-power wireless ad hoc networks in details. The equipment takes ATmega16L signle-chip microcomputer as master chip, achieves wireless transceiver by RF transceiver chip NRF24L01, and uses planar network topology and on-demand routing mode. It realizes collection of mine environmental data and early warning, reduces monitoring blind spots, and has higher security and reliability.

To clarify ecological environment quality and its influencing factors, we constructed an improved remote sensing ecological index (ISREI) coupled with greenness, humidity, dryness and aerosol optical depth based on satellite remote sensing data. We evaluated the ecological environment quality of Luanhe River Basin by IRSEI, and investigated the influencing factors. The results showed that IRSEI were 0.488, 0.532, 0.563, and 0.586 in 2000, 2008, 2016, and 2022, respectively, indicating that ecological environment quality of the basin continued to rise. The ecological environment quality and its change were characterized by high in southeast and low in northwest, improvement in southeast and degradation in northwest. Annual precipitation, slope, land use, primary industry and tertiary industry were the key factors affecting the spatial variations of ecological environment quality. The interaction of these factors had a more significant impact on the ecological environment quality.

Drought is one of the main disasters causing agricultural production losses. Accurate and effective agricultural drought monitoring is of great significance to reduce agricultural production losses. In this study, the standardized soil moisture index for agricultural drought evaluation was established by using the historical time series of soil moisture. Based on the index, the temporal and spatial distribution of drought events in the Luanhe River Basin from 2002 to 2019 were analyzed, and the agricultural drought was quantitatively evaluated. The results showed that soil moisture of most grid units in the Luanhe River Basin obeyed normal distribution during the study period, and that the soil moisture of a few grid units obeyed generalized extreme distribution. The changes of drought and flood in each grid unit had good temporal consistency, that is, the changes of drought and flood was consistent. The changes of drought and flood in the middle of the basin was relatively significant, while the agricultural drought generally showed a trend of reduction. The spatial distribution of agricultural drought severity was not obvious. Relatively speaking, the drought characteristics (the frequency, duration and intensity of drought) were higher in the middle and northwest of the basin and lower in the southeast. The results could provide a reference for agricultural drought prediction and the formulation of drought prevention and mitigation measures in the Luanhe River Basin.

To discuss the advantages and necessity of hidden curriculum construction in the academic experience inheritance of distinguished TCM veteran doctors by analyzing the characteristics of the hidden curriculum and the academic experience inheritance of distinguished TCM veteran doctors, and put forward viewpoints and pathways of promoting academic experience inheritance of distinguished TCM veteran doctors through the construction of hidden curriculum，such as optimal design of teaching environment，reasonable planning of teaching content and development of teaching information model，to effectively improve student cultivation quality and achieve the TCM talent cultivation goal which clinical diagnosis and treatment skills and clinical innovation ability are the core.

Objective: To evaluate the effect and cost-effectiveness of three commonly used molluscicides, 4% "Luo-wei" (tea-seed distilled saponins, TDS), 50% niclosamide ethanolamine salt wettable powder (NESWP), and 26% metaldehyde and niclosamide suspension concentrate (MNSC) in large-scale field application, so as to provide the references for formulating the strategy of snail control., Methods: The field test and parallel comparison were implemented. A marshland with Oncomelania hupensis snails of the Yangtze River was divided into 4 parts (10 hm 2 ) for the research, and three of them were experimental areas while the last one was a blank control area. The experimental areas were sprayed with 4% "Luo-wei", 50% NESWP and 26% MNSC respectively for 3 times and the interval was 1 week. Seven days after each spraying the effect of snail control was investigated, and the costs of molluscicides, labor, transportation, fuel consumption and mechanical loss were recorded. The cost of each molluscicide, snail mortality, snail density, and the cost of increasing 1% of snail mortality per 100 m 2 were analyzed and compared., Results: After the first, second and third spraying, the corrected snail mortality rates were 67.34%, 76.55% and 84.60% respectively in the 4% "Luo-wei" group; the corrected snail mortality rates were 64.71%, 75.17% and 83.89% respectively in the 50% NESWP group; the corrected snail mortality rates were 66.55%, 76.27% and 86.67% respectively in the 26% MNSC group. There was no significant difference among the 3 groups in the snail mortality at the same spraying time (χ 2 = 1.590, 0.571, 3.238, all P > 0.05) . In addition, along with the increase of the spraying times, the snail mortality of each group was increased significantly compared to that of the control group (χ 2 = 79.333, 94.718, 117.020, all P < 0.01) . After the first, second and third spraying, the reduction rates of snail density were 69.82%-86.60% in the 4% "Luo-wei" group, 68.66%-86.55% in the 50% NESWP group, and 71.89%-88.87% in the 26% MNSC group respectively. The decreasing amplitude of the snail density was more than 85% in all the experimental areas after 3 rounds of spraying molluscicide. The snail control costs per 100 hm 2 were 19.57, 11.97 Yuan and 10.47 Yuan in the 4% "Luo-wei" group, 50% NESWP group, and 26% MNSC group respectively. After the first, second and third spraying, the costs of increasing 1% of snail mortality per 100 m 2 were 0.30, 2.08 Yuan and 2.38 Yuan in the 4% "Luo-wei" group, 0.20, 1.10 Yuan and 1.32 Yuan in the 50% NESWP group, and 0.17, 1.04 Yuan and 0.97 Yuan in the 26% MNSC group respectively, and the cost-effectiveness was the highest at the first spraying in all the three groups., Conclusions: The effects of the three molluscicides for snail control are similar, but the efficacy of snail control is reduced as the spraying time increases.

Calculation of design drainage modulus is of great importance for the safety of farmland and reduction of waterlogging loss. We investigated the effects of main meteorological factors on design farmland drainage modulus by focusing on a closed farmland area in Ninghe District of Tianjin City. To resolve the problem of limited observation, the parameter sensitivity was analyzed according to mutual information and partial rank correlation coefficient aiming at the total runoff depth based on field observation experiment. The reasonable parameters were obtained by combining with soil water content and the relative variation of groundwater level. Then, a three-source runoff yield model coupling of excess infiltration and saturation was established to estimate runoff and design drainage modulus, and the effects of meteorological factors such as precipitation and evaporation on drainage modulus were analyzed. Results showed that summer precipitation significantly reduced and evaporation varied a little during 1960-2015. The corresponding drainage modulus decreased with the reduction of precipitation. When return period increased, the magnitude of the reduction in precipitation as well as the design drainage modulus increased. However, the effects of meteorological factors on design drainage modulus was generally inapparent. Thus, we concluded that the current drainage modulus is suitable for the study area.

Objective: To evaluate the clinical effects of percutaneous pedicle screw fixation(PPSF) combined with percutaneous vertebroplasty(PVP) for the treatment of osteoporotic thoracolumbar fractures., Methods: The clinical data of 94 patients with osteoporotic thoracolumbar fractures treated from January 2014 to December 2015 were retrospectively analyzed. There were 31 males and 63 females, aged from 65 to 70 years old with an average of 67.2 years. Fracture level was T₁₁ on 15 cases, T₁₂ on 32 cases, L₁ on 29 cases and L₂ on 18 cases. The patients were divided into two groups according to different therapeutic methods. Percutaneous pedicle screw fixation combined with percutaneous vertebroplasty were applied in 43 patients(group A) and percutaneous vertebroplasty was applied to 51 patients(group B). Operation time, intraoperative blood loss, bone cement volume, postoperative in-bed time were recorded; preoperatively, 3 d, 1 year after the operation, the ratios of anterior border heights, sagittal Cobb angles, visual analogue scale(VAS) scores were compared between two groups. The condition of postoperative complication in two groups was analyzed., Results: All the patients were followed up for 12 to 24 months with an average of 18.5 months. Operation time of group A [(96.2±28.7) min] was longer than that of group B [(31.8±10.6) min]. Intraoperative blood loss of group A[(62.2±25.5) ml] was more than that of group B [(25.4±10.9) ml]. Bone cement volume of group A [(5.5±0.5) ml] was larger than that of group B [(4.9±1.1) ml]. Postoperative in-bed time of group A[(5.1±1.8) d] was longer than that of group B[(1.8±0.7) d]. There were significant differences in operation time, intraoperative blood loss, bone cement volume, postoperative in-bed time between two groups( P <0.05). Three days, 12 months after the operation, the ratios of anterior border heights and Cobb angles in two groups were significantly improved. At final follow-up, the ratio of anterior border height and Cobb angle of group A[(85.6±3.5)%, (11.9±5.3)°] were better than of group B[(84.2±4.5)%, (15.3±3.4)°]( P <0.05). Three cases in group B had re-collapse of cemented vertebral bodies. Postoperative at 3 d, 1 year, VAS score of all patients had significantly decreased( P <0.05), and there was no significant difference between two groups( P >0.05)., Conclusions: Compared to simple PVP, PPSF combined with PVP in treating osteoporotic thoracolumbar fracture can obtain stronger vertebral strength and stiffness, furthermore to improve vertebral reduced effect, keeping vertebral heights, and preventing vertebral re-collapse., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© 2018 by the China Journal of Orthopaedics and Traumatology Press.)

Objective: To evaluate the potential risk of schistosomiasis transmission in the area along the Yangtze River in Yangzhou City, so as to provide evidences for establishing a post-transmission surveillance system for schistosomiasis in marshland regions., Methods: The water infectivity, floating boatmen and fishermen infection, reservoir host infection and wild feces contamination were investigated in five districts/counties along the Yangtze River in Yangzhou City, including Guangling, Hanjiang, Jiangdu, Yizheng and Development Zone, and the transmission factors and risky characteristics were assessed after interruption of schistosomiasis transmission in marshland regions., Results: A total of 15 key water regions were identified in the area along the Yangtze River in Yangzhou City in 2015. A total of 1 500 sentinel mice were placed, after breeding, their overall survival rate was 99.33%; 1 490 were dissected, with no schistosome infection. Of the 5 576 floating boatmen and fishermen examined, no schistosome infection was observed, and among the 3 566 domestic animals (including 171 cattle, 1 895 sheep and 1 500 pigs), no infections were detected. During the period between January and March, 2016, there were 3 200 mouse traps placed on 8 marshlands, and 62 wild mice were captured from 6 marshlands, with a capture rate of 1.94%, and no schistosomeinfected wild mice were seen. In addition, there were 35 pieces of fresh wild feces captured from 7 marshlands, including 11 pieces of bovine feces (31.43%), 17 pieces of sheep feces (48.57%), 2 pieces of dog feces (5.71%) and 5 pieces of other feces (14.29%), and no infections were detected., Conclusions: There is a low risk of schistosomiasis transmission in the area along the Yangtze River in Yangzhou City. However, the contamination of feces from bovine and sheep that are freely pastured on marshlands is a big threat to schistosomiasis control.

Objectives: To investigate the influence of interferon-alpha-2b (IFN-α2b) with JAK2 kinase, COX-2 and microvessel density in patients of MPN and the relation of JAK2V617F and COX-2 in human erythroleukemia cell line (HEL) cells., Methods: Forty-two cases of MPN patients with JAK2V617F mutation of initial treatment were collected from the Frist hospital of Baoding, including the IFN-α2b treatment group with 17 cases and untreated group with 25 cases. 10 cases of idiopathic immune thrombocytopenic purpura (ITP) patients synchronization were enrolled as controls. JAK2V617F/JAK2 mutation burden of MPN patients was detected by real time PCR (qRT-PCR);the expression levels of p-JAK2, COX-2 and microvascular density (MVD) marked with CD105 inpathological tissues of bone marrow in patients of MPN and ITP were detected by immunohistochemistry. The HEL cells were treated with different concentrations of IFN-α2b. The cell proliferation inhibition rate was calculated by CCK-8 test;the apoptosis rate was detected by flow cytometry; cell migration ability was tested by transwell chambers. JAK2 and COX -2 mRNA were detected by semi-quantitative PCR; p-JAK2 and COX-2 protein in HEL cells were detected by Western blotting., Results: The expression levels of p-JAK2, COX-2 protein and MVD in untreated group were significantly higher than those of control groups. p-JAK2, COX-2 and MVD levels were significantly reduced in patients treated with IFN-α2b. Cell growth inhibition rates and apoptosis rates raise up by dose of IFN-α2b in HEL cells at 48 h.The mRNA expression levels of JAK2 and COX -2 as well as protein expression levels of p-JAK2 and COX-2 had a decreasing tendency with the increase of IFN-α2b concentration at 48 h.The migration capacity level of HEL cells which treated with 0.5×10 4 U/L IFN-α2b after 24 h was lower than that of control group., Conclusions: Angiogenesis of MPN and COX-2 were inhibited by IFN-α2b which regulates JAK2 signal pathway.

Objective: To explore the correlation between the genetic dissimilarity and heterozygosity of mates and the pathogenicity of Schistosoma japonicum in the definitive host., Methods: By using seven microsatellite loci markers, S. japonicum genotyping of sixteen pairs randomly mated was performed, the genetic dissimilarity and heterozygosity were calculated between the mates, and the correlation between the genetic dissimilarity and heterozygosity of the mates and the pathogenicity of S. japonicum in the definitive host was evaluated., Results: There was a significant correlation between the genetic similarity of S. japonicum mates and the mean number of eggs per worm pair in the liver and intestinal tissue ( r = 0.501 6, P < 0.05; r = 0.796 5, P < 0.01, respectively) and the hatching rate of deposited eggs in the liver ( r = 0.508 3, P < 0.05), respectively. There was no correlation between the genetic similarity of the mates and hepatosplenomegaly per worm pair ( r = 0.109 5, P > 0.05; r = 0.265 3, P > 0.05, respectively) and the average diameter of granuloma in the liver ( r = -0.272 7, P > 0.05), respectively. There was no correlation between the heterozygosity of the mates and all the pathological parameters of S. japonicum in the definitive host ( P > 0.05)., Conclusions: There is the correlation between the genetic dissimilarity of the mates and the pathogenicity of S. japonicum in the definitive host, and the genetic dissimilarity is greater, pathogenicity is weaker. There is no correlation between heterozygosity of the mates and the pathogenicity of S. japonicum in the definitive host.

Objective: To investigate the clinical value of ultrasound markers in screening fetal trisomy 21., Methods: From Jan. 2001 to Dec. 2011, a retrospective study about sonographic information of 138 fetuses diagnosed as trisomy 21 was taken in the First Affiliated Hospital of Sun Yat-sen University. All fetuses were divided into 3 groups: isolated ultrasound markers, non-isolated ultrasound markers, and isolated structural malformations or other abnormalities. The relationship between trisomy 21 and ultrasound markers as well as structural anomalies or other abnormalities was analyzed., Results: Sonographic anomalies were detected in 132 fetuses (95.7%, 132/138), including ultrasound markers and structural malformations or other abnormalities. One hundred and twenty cases (87.0%, 120/138) had ultrasound markers, 38 (31.7%, 38/120) had one marker and 82 (68.3%, 82/120) had more than one marker (P < 0.01). Fifty-one fetuses (37.0%, 51/138) had isolated ultrasound markers and non-isolated markers were found in 69 fetuses (50.0%, 69/138). Only 12 fetuses (8.7%, 12/138) had isolated structural malformations or other abnormalities. In 20 fetuses on whom the first-trimester ultrasound screening were performed, all had ultrasound markers, 95% (19/20) had thickened nuchal translucency and 55% (11/20) had nasal bone hypoplasia. The most common ultrasound markers on the second-trimester screening were nasal bone hypoplasia, which accounted for 41.9% (52/124) cases, followed by thickened nuchal fold (25.0%, 31/124), short femurs and humerus (24.2%, 30/124), echogenic intracardiac focus (16.1%, 20/124), mild ventriculomegaly (15.3%, 19/124), hyperechoic bowel (12.9%, 16/124), mild renal pyelectasis (12.1%, 15/124). Furthermore, the common structural malformations or other abnormalities were as follows: cardiac defects (33.1%, 41/124), digestive system (26.6%, 33/124)., Conclusions: Ultrasound markers are valuable for screening fetal trisomy 21. The fetuses of trisomy 21 usually had more than one ultrasound markers or associated with other abnormalities. Combinations of ultrasound markers with the results of serum screening and maternal age are necessary for evaluation.

Objective: To perform spectral karyotyping (SKY), fluorescence in situ hybridization (FISH) and conventional karyotyping on prenatally detected marker chromosomes and complex chromosomal aberrations., Methods: Five marker chromosomes and 2 complex chromosome aberrations diagnosed by G banding were collected. SKY was performed to verify the composition of marker chromosomes. FISH was used to confirm the diagnosis when necessary. In certain cases, C or N banding technique was employed to verify the composition of chromosomes. Results of ultrasonography and pregnancy outcome were reviewed., Results: Among the 5 marker chromosomes, 2 were large and 3 were medium in size, 4 were de novo and one was inherited from the father. By SKY analysis, 2 marker chromosomes have originated from non-acrocentric chromosomes (4 and 9), whilst the other two have originated from acrocentric chromosomes (21 and 22). The remainder was derived from X chromosome. The SKY results were confirmed by FISH in 3 cases. Four cases have chosen to terminate the pregnancy after genetic counseling. A fetus with inherited paternal marker chromosome was delivered at term, and showed normal development during the first year of life. As for the other 2 cases with complex chromosome aberrations, by SKY examination, one had duplication in chromosome 8 and the other had chromosome rearrangements derived from translocation between chromosomes 2 and 6. In the latter case the fetus was delivered at term but showed developmental retardation at 6 months., Conclusion: SKY in combination with FISH can facilitate identification of the origins of marker chromosomes as well as complex chromosomal aberrations. With combined information from ultrasonography, SKY and FISH, effective counseling may be offered to the patients.

To analyze 25 nucleoprotein gene (N gene) sequences of rabies viruses circulating in Guizhou province during 2005-2010, China, and to explore the epidemic characteristics and the probable mutant of rabies in Guizhou Province. Rabies virus RNA in human brain tissues, human saliva, and domestic dog brain tissues derived from different prefectures of Guizhou Province were detected with RT-nested PCR, and the amplified products were then sequenced. Bioinformatics software was used to determine the genetic characteristics of these rabies viruses. The sequences of N gene of 25 Guizhou provincial isolates were identical with homogeny between 97.5% - 99.3% and 98.4% - 99.8% at nucleotide and deduced amino acid level, respectively, while the identities between them and isolated strains from other province of China were 88% - 99.1% and 88% - 99.7%. There were several amino acid substitutions in the nucleoprotein of 25 Guizhou isolates compared with the known genotype 1 isolates. The analysis of phylogenetic tree of 25 Guizhou isolates was demonstrated to be genetically divided into two groups, indicating that the virus presented a unique characteristics in geographic distribution and in a time dependent-manner. And phylogenetic tree of 25 Guizhou isolates and 7 genotype 1 strains isolated from other Province of China was also divided into two groups, which were further composed of several subgroups, respectively. From these observations, the rabies viruses derived from Guizhou province were still genotype 1. These isolates of rabies virus were diverged from the strains isolated from other provinces in both gene sequences and deduced amino acid sequences, and these divergences were characterized in geographic distribution and in a time-dependent manner.

Objective: Comprehensive use of molecular cytogenetic techniques for the detection of 1 case of small chromosome translocation., Methods: Following conventional chromosome preparation, G-banding karyotype analysis, spectral karyotyping (SKY), whole chromosome painting, two-color fluorescence in situ hybridization (FISH) and subtelomeric probe FISH were performed., Results: G-banded karyotype was 46, XX, ?(22q11.3), SKY karyotype analysis was 46, XX, der (4)t(4;6) and found no abnormalities on chromosome 22, staining signal was not found with any abnormalities on chromosome 6. Two-color FISH indicated a chromosomal translocation segment of 22q13.3 to one end of the short arm of chromosome 4. Subtelomeric FISH probe showed the end of the long arm of chromosome 22 and the end of the short arm of chromosome 4 reciprocal translocation. High resolution G-banding and FISH result indicated 46, XX, t(4;22)(p15.3;q13.2)., Conclusion: The testing of small chromosomal translocation should be combined with clinical information and integrated use of molecular cytogenetic techniques to improve the accuracy of diagnosis of chromosomal diseases.

Objective: To evaluate the expression of N-cadherin and β-catenin protein and their relationship with clinicopathological characteristics of osteosarcoma., Methods: The expressions of N-cadherin and β-catenin at protein level were detected by immunohistochemical staining in 54 cases of osteosarcoma, 11 cases of osteoid osteoma, 7 cases of osteoblastoma and 8 cases of newly formed bone in nonmalignant bone diseases. The relationship between the two indexes and clinicopathological characteristics of osteosarcoma was analyzed., Results: In newly formed bone, osteoblastoma, osteoid osteoma and osteosarcoma, the positive expression rate of N-cadherin protein was 75.0%, 71.4%, 63.6% and 35.2%, respectively. The positive expression rate of N-cadherin protein in osteosarcoma was significantly lower than that in osteoid osteoma, osteoblastoma and newly formed bone in nonmalignant bone diseases (P = 0.035). The positive expression rate of N-cadherin protein in osteosarcoma cases with pulmonary metastasis was lower than that in cases without (21.7% vs. 56.3%, P = 0.027). The positive expression rate of N-cadherin protein in osteosarcoma cases died in two years was lower than that in cases lived for more than two years (18.2% vs. 50.0%, P = 0.024). In newly formed bone, osteoblastoma, osteoid osteoma and osteosarcoma, the aberrant expression rate of β-catenin protein was 12.5%, 28.6%, 27.3% and 66.7%, respectively. The aberrant expression rate of β-catenin protein in osteosarcoma was significantly higher than that in osteoid osteoma, osteoblastoma and newly formed bone (P = 0.002). Aberrant expression rate of β-catenin in osteosarcoma cases with pulmonary metastasis was higher than that without (82.6% vs. 43.8%, P = 0.011). An inverse correlation was found between the aberrant expression of β-catenin and N-cadherin expression in osteosarcoma(r = -0.302, P = 0.027)., Conclusion: The positive expression rate of N-cadherin is decreased in osteosarcoma while aberrant expression rate of β-catenin increased. The expression of N-cadherin protein is closely correlated with the metastasis and prognosis of osteosarcoma, but the expression of β-catenin protein is merely correlated with the metastasis of osteosarcoma.

Satellite sensor technology endorsed better discrimination of various landscape objects. Image segmentation approaches to extracting conceptual objects and patterns hence have been explored and a wide variety of such algorithms abound. To this end, in order to effectively utilize edge and topological information in high resolution remote sensing imagery, an object-oriented algorithm combining edge detection and region merging is proposed. Susan edge filter is firstly applied to the panchromatic band of Quickbird imagery with spatial resolution of 0.61 m to obtain the edge map. Thanks to the resulting edge map, a two-phrase region-based segmentation method operates on the fusion image from panchromatic and multispectral Quickbird images to get the final partition result. In the first phase, a quad tree grid consisting of squares with sides parallel to the image left and top borders agglomerates the square subsets recursively where the uniform measure is satisfied to derive image object primitives. Before the merger of the second phrase, the contextual and spatial information, (e. g., neighbor relationship, boundary coding) of the resulting squares are retrieved efficiently by means of the quad tree structure. Then a region merging operation is performed with those primitives, during which the criterion for region merging integrates edge map and region-based features. This approach has been tested on the QuickBird images of some site in Sanxia area and the result is compared with those of ENVI Zoom Definiens. In addition, quantitative evaluation of the quality of segmentation results is also presented. Experiment results demonstrate stable convergence and efficiency.

Objective: To study the expression of EP-CAM, beta-catenin in the carcinogenesis of squamous cell carcinoma of uterine cervix., Methods: The expressions of EP-CAM and beta-catenin were detected with immunohistochemical stain in 14 cases of normal cervical squamous epithelium, 32 cases of cervical intraepithelial neoplasia (CIN) and 38 cases of cervical invasive squamous cell carcinoma., Results: The over-expression rates of EP-CAM were 0, 7.1%, 20.0%, 62.5% and 55.3% for normal cervical epithelium, CINI, CINII, CINIII and carcinoma groups. The EP-CAM over-expression rates in CINIII and cervical carcinoma groups were significantly higher than those in normal epithelium and CINI groups (P < 0.001). No aberrant expression of beta-catenin was shown in normal cervical epithelium, while the aberrant expression rates of beta-catenin in CINI, CINII, CINIII and cervical carcinoma group were 28.6%, 40.0%, 62.5% and 84.2%. The aberrant expression rate of beta-catenin increased with the increase in degree of CIN and development of cervical carcinoma. The over-expression rate of EP-CAM was reversely related to the differentiation of cervical squamous cell carcinoma (P < 0.001)., Conclusion: EP-CAM and beta-catenin may be involved in the carcinogenesis of squamous cell carcinoma of uterine cervix. The over-expression of EP-CAM and aberrant expression of beta-catenin may serve as markers of squamous carcinogenesis of uterine cervix.

Background & Objective: Cixian County is one of the high incidence areas of esophageal carcinoma in China. Up to now, no work has been conducted on the possible etiological role of human papilloma virus (HPV) infection on esophageal carcinoma in this local area. The aim of this study was to explore the putative roles of HPV infection on the esophageal carcinogenesis of the patients in Cixian and to elucidate the possible relationship between HPV existence and fragile histidine triad gene (FHIT gene, a putative tumor suppressor gene), expression in squamous cell carcinoma of esophagus., Methods: The existence of HPV DNA and the expression of FHIT gene at protein level in esophageal carcinoma tissues were determined with PCR and immunohistochemical staining, respectively, in 128 archival paraffin-embedded tissue blocks of esophageal squamous carcinoma from the high incidence area of Cixian and 24 tissue blocks from the non-high incidence area., Results: PCR results showed that the positive detection rate of HPV in esophageal carcinoma tissues from the high incidence area was 20.3%, which was slightly higher than that from the non-high incidence area (8.3%)(P >0.05). Immunohistochemically, aberrant expression of FHIT gene in esophageal carcinoma tissue was found in 75.6% cases from the high incidence area and only 54.2% from the non-high incidence area, which was significantly lower than that of the former (P< 0.05). No correlation was found between the abnormal expression of FHIT and existence of HPV DNA in esophageal squamous carcinoma tissues., Conclusion: HPV DNA could be detected in partial esophageal carcinoma cases from the high incidence area of esophageal carcinoma in Cixian. The aberrant expression rate of FHIT protein in the cases from the high incidence area of esophageal carcinoma in Cixian is higher than that from the non-high incidence area.