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BackgroundThere are a variety of microorganisms in ambient air, and susceptible people can be infected once contact with pathogenic microorganisms in the environment. In order to avoid the spread of pathogenic bacteria, disinfection is the simplest and most effective way of killing pathogenic bacteria in the environment to block the contact between pathogenic bacteria and humans. Sodium hypochlorite (NaClO) is the most widely used disinfectant, but its safety in ambient air disinfection is not clear yet. ObjectiveTo establish a model of bronchial epithelial cell (BEAS-2B) injury induced by NaClO, and to explore the mechanism of the toxic effect of NaClO disinfectants on BEAS-2B. MethodsCells were treated with concentration gradients of 0, 25, 50,100, 200, and 400 μmol·L−1 of the diluted NaClO (100 mmol·L−1) standard solution, respectively, and cell activity was measured by cell counting kit-8 (CCK-8) assay after 15 and 30 min. Cells treated with 0, 25, and 50 μmol·L−1 NaClO were selected to observe the cell morphology under an inverted microscope, apoptosis was determined by flow cytometry Annexin V FITC / PI double staining to determine the final experimental concentration. The morphology of organelles such as mitochondria was observed under a transmission electron microscope. Mitochondrial membrane potential of the cells was detected by JC-1 staining. Intracellular Ca2+ concentration was measured with a Fluo-4 AM fluorescent probe. Total cellular reactive oxygen species (ROS) was detected with a 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe, cell mitochondrial ROS with a dihydroethidium (DHE) fluorescent probe, and lipid peroxidation intermediate malondialdehyde (MDA) with a commercial kit. ResultsCompared with 0 μmol·L−1, NaClO treatment group, cell morphology did not change a lot after 25 μmol·L−1 NaClO treatment for 30 min, and the cells began to wrinkle and become round after 30 min treatment with 50 μmol·L−1 NaClO, showing about 70% of normal cell viability (P

To evaluate the degree of unsaturation of different varieties of Camellia oil, it was necessary to establish a rapid evaluation method with a narrow iodine value range (iodine value difference less than 10). In this study, a rapid quantitative prediction model for iodine value of oil in high-resolution Raman spectroscopy based on linear regression and gradient descent method was established. The Raman signals (785 nm) about 39 group of Camellia oil samples and 10 group of commercially oils were firstly collected. Then, the intensity ratio of peaks of 1656 cm−1 and 1440 cm−1 (I1656/1440) were selected through smoothing algorithm least squares smoothing filter (Savitzky-Golay), polynomial fitting and deconvolution algorithm Lorentzian. A credible model was obtained through correlation analysis with the iodine value of corresponding oil samples. The coefficient of determination (R2) of the test set of the constructed quantitative model was >0.82, the mean square error (MSE) was

In order to address the issue of high development of hard overlying rock water-conducting fracture zones in deep coal mining and achieve water-preserved coal mining by reducing the height of water-conducting fracture zones, a new method is proposed in this study, which combines the techniques of pre-fracturing and weakening of hard main controlling overlying strata. This study comprehensively investigates the modification effect of pre-fracturing and weakening of hard main controlling overlying strata through laboratory experiments, theoretical analysis, and numerical simulation. The control mechanisms of different weakening layers on water-conducting fracture zones are elaborated and validated through field measurements. The results demonstrate that pre-existing fractures can induce the rock mass to shift its failure mode from intact rock fracturing to tension-shear failure controlled by pre-existing fractures, leading to a reduction in the severity of rock mass failure. The weakening effect of pre-existing fractures on the hard rock mass is revealed, and the damage variables coupled with stress and fractures are calculated. The relationship between the degree of weakening and the transformation of rock properties is qualitatively analyzed. It is found that the energy storage capacity of the rock mass is reduced while the dissipation capacity is enhanced after the modification. Numerical simulations are conducted to evaluate the control effect of different weakening layers on water-conducting fracture zones. By comparing the dynamic evolution of overlying rock failure modes, fracture counts, and failure heights, it is observed that the “saddle-shaped” failure mode gradually weakens with the increase of weakening layer height, and the evolution trend of fracture counts follows a pattern of “slow increase-sudden increase”. The dynamic evolution of failure height for unweakened and moderately to highly weakened overlying strata approximately exhibits an “S” shape, while the moderately weakened overlying strata exhibit a “semi-ejection” shape. Based on the analysis of the characteristics of failure in hard rock layers at different positions, the mechanisms of controlling the development of water-conducting fracture zones by different pre-fracturing weakening layers are revealed. Furthermore, using the borehole measurement method, the development height of the “two zones” under the condition of top plate fracturing in Mengcun mine is obtained. It is observed that the fracture extraction ratio is reduced under the condition of pre-fracturing weakening, thus preliminarily validating the inhibitory effect of top plate pre-fracturing weakening on the development of water-conducting fracture zones.It provides theoretical and scientific basis for coupling disaster prevention and control, water resources and ecological protection。

ObjectiveAutonomous docking is the key to the cooperative operation of unmanned underwater vehicles (UUVs). However, due to environmental complexity and object characteristics, it is very difficult to achieve precise guidance and docking. In order to improve the accuracy and robustness of underwater docking, this study proposes a vision-guided docking scheme which encompasses vision processing and 3D trajectory tracking control. MethodsFirst, the overall vision-guided docking scheme is designed in combination with an analysis of task and object characteristics. Second, the YOLOv5 neural network is designed to complete the target detection of the underwater docking station, and the online measurement of the relative position and attitude relationship between the docking station and UUV is realized by an efficient perspective-n-point (EPnP) algorithm. Next, combined with the visual measurement results, an effective 3D robust trajectory tracking controller is designed on the basis of the 3D LOS guidance law, radial basis function neural network (RBFNN) and terminal sliding mode control (TSMC). Finally, the validity of the proposed scheme is verified through numerical simulation and a tank test. ResultsIn the tank test, the proposed vision-guided control algorithm can effectively complete the online detection and relative positioning of the underwater docking station, thereby achieving precise underwater docking. ConclusionThe results of this study show that the proposed vision-guided 3D trajectory tracking control scheme is reasonable and efficient, and can lay a good foundation for UUV docking.

Objective To explore the association between whole blood lead concentration and advanced colorectal neoplasia (CRN) and to provide evidence for colorectal cancer (CRC) prevention and control. Methods The cases of the study were 272 advanced CRN identified from April 2014 to December 2016 in the participants of a CRC early diagnosis and treatment program including face-to-face questionnaire interview, physical examination and laboratory test conducted among the residents of Jiashan county, Zhejiang province; the 605 controls were gender- and age (± 5 years)-frequency matched healthy participants of the program. Results The geometric mean (geometric standard error) of whole blood lead concentration (μg/L) were 28.11(1.64) and 26.87 (0.93) for the cases and controls, without statistically significant difference (P > 0.05). After adjusting for gender, age, education, smoking, alcohol consumption, physical exercise, family history of CRC and body mass index (BMI), unconditional multivariate logistic regression analysis demonstrated that the participants with the highest quintile (Q5) of whole blood lead concentration were at an increased risk of advanced CRN (odds ratio [OR] = 1.87, 95% confidence interval [95%CI] : 1.15 – 3.04) compared to those with the lowest quintile (Q1) and the increased risk was much higher for the male participants (Q5 vs. Q1: OR = 2.12, 95%CI : 1.05 – 4.30). Furthermore after base 10 logarithm conversion, each unit increment in blood lead concentration was associated with a 1.86 (OR = 2.86, 95%CI : 1.28 – 6.35) and 3.98 (OR = 4.98, 95%CI : 1.62 – 15.35) times higher risk of advanced CRN for the all and the male participants, respectively. Restricted cubic spline analysis revealed a significant linear correlation between the increment of whole blood concentration and the increased risk of advanced CRN after adjusting for potential confounders mentioned above (χ2 total = 8.79, Ptotal = 0.012; χ2 non-linearity = 2.17, Pnon-linearity = 0.141). ConclusionBlood lead concentration may be associated with an increased risk of advanced colorectal neoplasia, suggesting a potential role of lead exposure in the etiology of colorectal carcinogenesis.

Objective To determine the volume range of suspended erythrocyte and establish its internal control standard. Methods The theoretical value of suspended erythrocyte volume was calculated according to the screening criteria of healthy blood donors and Quality Requirements for Whole Blood and Blood Components. A total of 2 410 bags of 1 U and 2 U suspended erythrocyte were randomly selected and weighed, and the volume range were formulated by x-±2S andx-±10% respectively and then compared to determine the volume range in line with the actual situation of our center. Results The theoretical volume range of 1 U and 2 U suspended erythrocyte were 117-160 mL vs 234-320 mL, and the actual volume range were 142-180 mL vs 276-393 mL. The volume range of 1 U and 2 U suspended erythrocyte formulated by x-±2S were 145-181 mL vs 298-358 mL, and by x-±10% were 147-179 mL vs 295-361 mL. The hematocrit and hemoglobin content of suspended erythrocyte within the actual volume range met the quality requirements. There were fluctuations in the volume of suspended erythrocyte from different regions. Conclusion Based on the actual situation of our center and the sampling results of suspended erythrocytes in recent two years, 163 mL±10% and 328 mL±10% were determined as the internal control standards of 1 U and 2 U suspended erythrocyte, respectively. Blood centers should establish accurate and feasible standard of suspended erythrocyte according to the actual situation.

Combining the advantages of Glaciology and seismology, Glacier seismology formes a young interdisciplinary subject. Icequakes refer to the vibration generated during the movement and rupture of a glacier, ranging from small creaks to sudden rupture or sliding equivalent to an earthquake (MW7). According to the location and mechanism of icequakes, the icequakes can be divided into five types: surface crevasses, stick-slip motion, iceberg calving, subglacial water flow and hydrofracturing. In addition to traditional seismological methods, icequake research can be used combing with multidisciplinary methods such as GPS, numerical simulation, and glacier physical properties. Icequake research can further explore the occurrence process and risk assessment of ice avalanches. We reviewed the research progress of glacier seismology. Moreover, we introduced part of the glacial earthquake research work carried out by domestic researchers in the Tibetan Plateau. Finally, we comprehensively discussed the enlightenments from glacier earthquakes to natural earthquake research.

Radiomics, a technology based on multimodal medical image processing and analysis, is able to extract automatically and analyze massive data from computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography/computed tomography (PET/CT) via high-performance computer algorithm in order to pursue early diagnosis of disease, benign and malignant tumor discrimination, dynamic evaluation of disease treatment, and individualized precision therapy. To date, many studies demonstrate that radiomics not only has great potential in early diagnosis of lung cancer and prediction of genotype, treatment efficacy, as well as prognosis but also is based on imaging methods that are noninvasive, inexpensive, and repeatable. It does demonstrate precious values in guiding the clinical diagnosis and treatment of lung cancer, especially in the personalized and precise treatments and researches of lung cancer. However, the consistency and reproducibility of radiomics and the selection of robust characteristics still warrant further researches.

Segment routing (SR) is a routing mechanism proposed by IETF in recent years,which aims to make the IP/MPLS network service-oriented and more efficient.SR eliminates the complex control plane protocol (LDP/RSVP-TE) of the MPLS network,while SR implements the traffic engineering mechanism.Different from the explicit path in the RSVP tunnel which maintains the state of each flow in the middle of the tunnel,the explicit path based on SR only maintains the state of flow at the entrance border router of the SR domain.For this reason,SR improves the scalability of the network.Therefore the research of the SR mechanism is very important to the next generation internet.The realization mechanism of SR was studied,using the click router.The SR control plane subsystem based on PCE architecture and each module of MPLS-based SR data plane subsystem were implemented and deployed in practice to verify the feasibility of the software router in actual deployment.

【Objective】Functional modifications of plant histones, including acetylation modification and methylation modification, play important roles in plant growth and development and in response to adversity. The biological functions and molecular mechanisms of the histone modification (HM) gene family in tomato are still unclear. The present study aims to further clarify the biological functions of the HM genes in tomato, and to lay a foundation for the study of their molecular mechanisms and genetic improvement of tomato.【Method】We identified tomato HM members based on the tomato genome database, systematically analyzed the phylogeny, gene structure, and chromosomal localization of its HM gene family members with bioinformatics methods, and analyzed the spatio-temporal expression patterns of the tomato HM gene family through an online transcriptome database.【Result】A total of 148 tomato HM genes were identified in this study, including 32 encoded histone acetyltransferase (HAT), 11 encoded histone deacetylase (HDAC), 50 encoded histone methyltransferase (HMT), and 55 encoded histone demethylase (HDM). The 148 genes were unevenly distributed on different chromosomes, with the highest number of genes on chromosome 3 (21 genes) and the lowest number of genes on chromosome 12 (4 genes). Gene structure characterization showed that the exons varied greatly among tomato HM genes, with the most exons amounting to 34 and the least to only 1. Protein structure domain analysis showed that Solyc07g064130, Solyc11g005670 and Solyc10g006480 contained only the Ubl structure domains, Solyc07g062100, Solyc10g077110 and Solyc03g083310 contained only the Zn-finger structure domain, another 10 proteins contain the Bromo structure domain, 48 proteins contained only the SET structure domain, and other proteins contained other structure domains such as PLN02529 and PRMT5. In addition, the homologous proteins of tomato HM were distantly related to those of both Arabidopsis and Oryza sativa, and the protein sequences of the tomato HM were more closely related to those of the Arabidopsis thaliana than to those of Oryza sativa. Based on the clustering analysis, the tomato HM members were divided into four families, HAT, HDAC, HMT, and HDM. Tissue expression analysis showed that the HAT family was highly expressed in flowers at 30 d after germination (F30), fruits at 10 d post-anthesis (10DPA), flowers (fr), roots (rr), and immature fruits (Plgfr); the HDAC family was highly expressed in flowers (F30) and immature fruits (Plgfr); the HMT family was highly expressed in flowers (F30), when 50% of the whole plant flowers open (F45), flower buds (br), 3 cm fruits (3fr), and immature 5 cm fruits (PB+5fr); the HDM family was highly expressed in flowers (F30), when 50% of the whole plant flowers open (F45), flower buds (br), flowers (fr), roots (rr), 3 cm fruits (3fr), and immature 5 cm fruits (PB+5fr).【Conclusion】The gene structures of different tomato HM vary greatly and contain multiple functional structural domains. The homologous members of tomato HM are distant from those of Arabidopsis thaliana and Oryza sativa, and tomato HM are expressed in different growth and developmental stages of plants and in different organ tissues, suggesting that this class of genes may be involved in a variety of growth and developmental processes in tomato.

Spontaneous combustion of coal in goaf is one of the main factors inducing mine fire, which accounts for 90% of mine fire. In order to achieve accurate prevention and control of spontaneous combustion in goaf, it is necessary to accurately obtain the high temperature point temperature in goaf. Based on Yanbei Coal Mine as the engineering background, this paper analyzes the index gases produced during the oxidation and heating process of coal samples, establishes the index system of coal spontaneous combustion temperature prediction, and then carries out the research of multi-index gas coal spontaneous combustion temperature prediction based on deep learning. Firstly, the coal samples collected from Yanbei Coal Mine were tested for coal oxidation and temperature rise. According to the experimental results, they were divided into single gas index and compound gas index. The change law of each index with temperature rise was analyzed, and the appropriate index was determined as the prediction index of coal spontaneous combustion temperature. The prediction index of coal spontaneous combustion temperature is processed by multi-source data processing method, the data is cleaned by Cook distance method and multiple interpolation method, and the prediction index system of coal spontaneous combustion temperature is established by grey correlation analysis method. Elman neural network was used to build a prediction model, and after determining the model structure and hyper-parameters, a prediction model of coal spontaneous combustion temperature was established to obtain an accurate prediction of the temperature in the process of coal oxidation and heating.

Objective In the current mainstream of train auxiliary power supply systems, such as cross-supply method and extended supply method, the failure of a single SIV (auxiliary inverter) can impact the normal operation of electrical equipment on the train. As an emerging control technology, the grid-connected power supply has its advantages and requires in-depth research into its control technology. Method Taking Shanghai Rail Transit Line 8 as example, issues related to slow response and low reliability in the auxiliary power supply system for the third batch of purchased train are addressed. For the design of the fourth batch of purchased trains, a dual-bus grid-connected power supply scheme based on TCMS (train control and management system) control is proposed. Specifically, four TLKs (grid-connected contactors) are arranged in the middle car to link four independent medium-voltage buses together. The medium-voltage bus structure of the auxiliary power supply system under this scheme is introduced, the action sequence during the internal start-up stages and grid-connected start-up stages of SIV are analyzed, and the logics of grid-connected power supply control, SIV start-up and fault protection are expounded. Result & Conclusion The time required for grid-connected power supply in the proposed scheme is significantly reduced (minimum of 2.5 seconds). The medium-voltage bus topology with dual-bus and multiple TLKs avoids system functionality overall failure due to bus short-circuits, improving the reliability of train auxiliary power supply system. The grid-connected power supply technology based on TCMS control is expected to be a development direction of auxiliary power supply technology for urban rail transit trains in the future.

A quick, easy, cheap, effective, rugged and safe pre-treatment method (QuEChERS) was established to evaluate the risk of tetracycline residues in the muscle of Channa argus. The Box-Behnken design (BBD) response surface optimization method was used to optimize the extraction and purification conditions. The samples were extracted with 10 mL acetonitrile (containing 1% formic acid) and 2 mL 0.1 mol/L Na2EDTA-Mcllvaine buffer solution, and the extracts were purified by 150 mg primary secondary amine（PSA）and 200 mg C18, and detected with Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The result showed that the calibration curve was linear in the range of 0.50~100 ng/mL, with high correlation coefficients (r≥0.999). The limits of detection (LOD) and quantification (LOQ) were 0.25～0.75 μg/kg and 0.84～2.00 μg/kg, respectively. The average recoveries of tetracycline, oxytetracycline, chlortetracycline and doxycycline at three different spiked levels in the muscle of Channa argus were 89.5%～118%, with relative standard deviations (RSDs) of 1.11%～8.88%. This method is simple, sensitive and accurate, and can be used for the rapid quantitative determination of tetracyclines in Channa argus.

【Objective】The study aimed to clarify the value of microbial fertilizer in the production of Chinese chive, and screen the best scheme for combined application of microbial fertilizer with chemical fertilizer in the high quality and safe production of Chinese chive and control of Bradysia odoriphaga.【Method】Xuejiu 791 was taken as the research object, 8 treatments of microbial fertilizer combined with chemical fertilizer were set up, namely, the recommended amount of chemical fertilizer combined with 30%, 50%, 70%, 100% of the recommended amount of microbial fertilizer (T2~T5), and the recommended amount of microbial fertilizer combined with 70%, 50%, 30%, and 0% of the recommended amount of chemical fertilizer (T6~T9). The effects of different fertilization schemes on the yield and growth traits of Chinese chives was estimated by indoor single factor experiment to screen the better fertilization schemes; and the effects of above better fertilization schemes on the yield and quality of Chinese chives and the occurrence of B. odoriphaga were verified by field experiments.【Result】The indoor experiment showed that the growth traits of Chinese chives were significantly improved by microbial fertilizer, with plant height, stem diameter, maximum leaf width, plant weight per plant and number of fibrous root significantly increased. Among all the fertilization schemes, the best growth traits of Chinese chives were found in T5, T6, and T7 treatment, especially for plant height, which was 27.87, 27.59 and 28.84 cm, respectively, and significantly differed from that in other treatments. The weight per plant was 0.99, 0.93 and 0.94 g, respectively, which was significantly different from that in other treatments except T4 and T9. The growth traits of Chinese chives did not differ among T5, T6 and T7. Based on the above results and the economic point of view, T6 and T7 were judged to be the optimal fertilizer scheme. The result of field experiment showed that the application of T6 scheme of indoor experiment (FT6 treatment) significantly increased the plant weight, stem diameter, pseudostem length, plant height, maximum leaf width and number of fibrous root of Chinese chives in greenhouse by 29.41%, 1.70%, 12.19%, 7.58%, 17.23% and 40.60%, respectively, compared to the control (FT1). The application of T7 scheme of indoor experiment in field (FT7 treatment) significantly increased the weight per plant, stem diameter, pseudostem length, maximum leaf width and number of fibrous root of Chinese chives by 23.22%, 4.21%, 11.94%, 9.47% and 16.99%, respectively, compared to the control (FT1), but the increase amplitude of each growth character was less than that in FT6. The yield of Chinese chives in FT6 and FT7 treatment was significantly increased by 48.39% and 12.99%, respectively compared to FT1. The quality of Chinese chives in FT6 and FT7 treatment was significantly improved, soluble sugar content and soluble protein content were increased by 32.72%, 10.45% and 6.76%, 6.60%, respectively, compared to FT1. The growth traits of Chinese chives in FT6 were better than those in FT7. FT6 and FT7 treatment inhibited the occurrence of B. odoriphaga, and the inhibition rates were 50.15% and 22.16%, respectively, compared to the control.【Conclusion】Compared with the single application of chemical fertilizer, the combined application of microbial fertilizer and chemical fertilizer effectively improve the yield and quality of Chinese chive, and inhibit the occurrence of B. odoriphaga. The best recommended scheme was 70% of the recommended amount of fertilizer combined with 100% of the recommended amount of microbial fertilizer.

【Objective】This study was conducted to investigate the effects of chemical fertilizer reduction combined with synergist on soil physicochemical properties, yield and quality of Chinese flowering cabbage in Huizhou, Guangdong Province, so as to provide a theoretical basis and technical support for chemical fertilizer reduction in vegetable fields.【Method】Taking the representative vegetable crop Chinese flowering cabbage in South China as the research object, four treatments of local fertilization level (T1, CK), local fertilization level+ synergist application (T2), 20% fertilizer reduction (T3) and 20% fertilizer reduction + synergist application (T4) were set up by field plot experiment. Soil physicochemical properties, microbial biomass, acid phosphatase activity, and crop yield and quality were measured and compared between treatments.【Result】The results showed that compared to T1 (CK), the soil capillary capacity, field capacity, non-capillary porosity, and total porosity in T3 treatment were increased by 13.3%, 43.1%, 29.4%, and 9.2%, respectively, and the soil non-capillary porosity and total porosity of T4 treatment were increased by 42.1% and 13.0%, respectively. Compared to T1 (CK), the soil pH value in T3 treatment was increased by 15.9%, whereas the other soil fertility indexes were not changed significantly. Under T4 treatment, soil pH value was increased by 13.4%, soil alkali-hydrolyzable nitrogen and available potassium contents were significantly increased by 33.3% and 15.8%, respectively. Compared to T1 (CK), the soil microbial biomass C (SMBC) and N (SMBN) contents and acid phosphatase activity in T3 treatment were increased by 35.6%, 43.1%, and 15.5%, respectively, whereas the SMBC, SMBN, and soil microbial biomass P (SMBP) contents and acid phosphatase activity in T4 treatment were increased by 78.1%, 111.2%, 49.1%, and 24.3%, respectively. Compared to T1 (CK), the N, P, and K absorption amounts of cabbage in T3 treatment were increased by 22.1%, 11.3%, and 17.9%, respectively, whereas those in T4 treatment were significantly increased by 33.0%, 28.5%, and 30.0%, respectively. Compared to T1 (CK), cabbage yield was increased by 19.4%, nitrate content was decreased by 13.6%, and Vc content was increased by 2.2% in T3 treatment. Cabbage yield was significantly increased by 28.1%, nitrate content was decreased by 12.1%, and Vc content was increased by 4.2% in T4 treatment.【Conclusion】Chemical fertilizer reduction combined with synergist application can increase soil fertility, microbial biomass and acid phosphatase activity, improve cabbage yield and quality.

[Objective] The ecological network of Natural Reserves in Lancang River basin, Yunnan Province was constructed in order to providing a scientific basis for the ecological security and protection of the basin. [Methods] We constructed a minimum accumulation resistance model of six ecological factors (distance from water, distance from town, vegetation coverage, land use, slope, and elevation). In this model, the city and county-level nature reserves were considered to be the ecological sources. The ecological network of the Lancang River basin was constructed as the least resistance path between different sources, identified as the potential ecological corridor to detect the ecological nodes and ecological fracture points. [Results] A total of 43 ecological sources in the Lancang River basin (mainly in the middle and lower reaches of the basin) were constructed by using the model, with 67 ecological corridors overlapping the main water system of the Lancang River by 59%. Twenty ecological nodes and 41 ecological breakpoints were identified in the ecological network, and these will be used in the optimization and restoration plan of the ecological network and the construction of regional roads in a later period. [Conclusion] According to the distribution characteristics of ecological source areas, ecological corridors, and ecological networks, the number of ecological source areas in the upper reaches of the Lancang River, Weiyuan River basin, and other related areas should be increased. At the same time, the quality of habitats along river banks in the Lancang River basin should also be improved through the use of ecological construction that considers ecological nodes and ecological breakpoints so as to form a complete ecological protection network, alleviate the problem of islanding, and promote the flow of species between ecological sources.

In this paper, the properties of purple rice powder were improved by mixing high amylose indica rice powder, and the effect of different proportions of mixed powder on the properties of raw materials and the quality of purple rice noodles was studied. The purple rice powder and the indica rice powder were mixed according to a certain proportion to make noodles. The physicochemical properties of mixed powder including amylose content, solubility, swelling power, gel consistency, gelatinization and gel properties were used as determination indexes. And the cooking, anthocyanin content and retention rate and sensory quality of purple rice noodles were used as evaluation indicators. The results showed that the optimum proportion of raw material powder was indica rice powder: purple rice powder=8:2. Under this ratio, the amylose content of the mixed powder increased from 15.75 g/100 g of pure purple rice powder to 26.86 g/100 g, the solubility decreased to 6.24 g/100 g, the swelling force decreased to 6.80 g/g, and the gel consistency decreased from the medium-soft consistency to hard consistency, the trough viscosity, final viscosity and retrogradation value increased, the gel hardness and water holding capacity increased, and the adhesiveness and weight loss rate decreased. The above-mentioned changes in the properties of the raw materials enhanced the structural strength of the purple rice noodles gel, reduced its stickiness, optimized its hydration properties, and finally improved the quality of the purple rice noodles. The quality of purple rice noodles made under the optimum ratio was as follows: The cooking time was 508 s, the broken strip rate was 9.67%, the cooking loss rate was 34.37%, the cooking quality was similar to the commercial rice noodles, the anthocyanin content was 18.36 mg/100 g, the retention rate reached 65.68%, and the color was purple, with the fragrance of purple rice, moderate hardness and softness.

5G convergence application is the key support for the digital development of China's economy and society.After more than two years of development, China's 5GtoB application has accelerated to bloom, has achieved a breakthrough from 0 to 1, and the enabling effect is obvious.The development of 5GtoB application needs to be further promoted from three directions: strengthening network supply, reducing application threshold and building application industry ecology.It is believed that in the future, 5GtoB applications will continue to develop and promote the wireless economy to become an important part of China's national economy.

In the two years since 5G was officially put into commercial use in China, the Central Government had made frequent statements and attached great importance to the development and application of 5G.All parties in the industry had made positive progress technical standards, network construction and industrial development, laying a solid foundation for the development of 5G industry applications.The development status and challenges of 5G industry applications were comprehensively analyzed, the pattern of 5G standard and technological innovation transformation was studied, the application development stage and future development path of the 5G industry based on the development rule of each industry sector was analyzed, and the large-scale development of the 5G industry applications was prospected.

The reliability of the planetary cycloidal reducer directly affects the overall performance and service life of industrial robots，among which the reliability of two-stage drive subcomponents has an important influence on the reliability of the whole planetary cycloidal reducer. Taking a heavy-duty planetary cycloidal reducer as the research object，the reliability mathematical model of each subcomponent is constructed based on the force analysis of two-stage transmission system subcomponents，and the reliability is correlated and coupled by Copula function to construct a system reliability model considering failure correlation； on this basis，a multi-objective optimization model with minimum volume and maximum reliability is constructed，and NSGA-Ⅱ（Non-dominated sorted genetic algorithm-Ⅱ） algorithm is used to solve the model，and it is compared and analyzed with the system reliability model considering the independence assumption. The results show that， after optimization，the volume of the reducer is reduced by 11.27%，and the reliability is improved by 5.29%，and the optimal design method considering failure correlation can effectively improve the reliability of the planetary cycloidal reducer with higher comprehensive performance.

Light Non-Aqueous Phase Liquids (LNAPLs) in vadose zone is of multi-phase characteristics, while factors such as heterogeneity and groundwater fluctuation are expected to significantly increase the complexity of LNAPLs contamination in vadose zone. Previous studies have mostly focused on revealing the contamination process of free-phase LNAPLs, few have explored deeply the influence of the heterogeneous structure on the migration and phase distribution pattern of LNAPLs when the water table fluctuates. A numerical model of multiphase flow in vadose zone is established based on TOUGH2 to reveal the migration and phase distribution of LNAPLs under the joint effect of different lithological lenses and water table fluctuation. The results show that (1) the migration and distribution regularity of LNAPLs in vadose zone is predominantly controlled by the variation in water content, which is presented under the effect of heterogeneity and water fluctuation. (2) In the steady groundwater scenario, LNAPL migrates in an “accumulation-lateral expansion-flow bypass” pattern around the fine-sand lens, while the coarse-sand lens acts as the "preferential route" for the vertical movement of LNAPL. Flow around the fine-sand lens is significantly enhanced by the variation in water content induced by groundwater fluctuation, and the coarse-sand lens further exhibits the "preferential space" effect. (3) When the water table is steady, LNAPL are concentrated inside and below the lens body in the fine-sand and coarse-sand lens models, respectively. In the fluctuating groundwater scenario, a greater range of LNAPL is presented in the vicinity of the lens with the distribution area in each model is 51% and 63% larger than that in the steady scenario. (4) Volatile flux of LNAPL, affected by the LNAPL-gas exposure conditions and the distribution of LNAPL, shows a "decreasing-then increasing" pattern in both models. The three-phase equilibrium state is disrupted by groundwater fluctuation, which is manifested by the enhanced volatilization during the stage of groundwater elevation, when the average volatile flux in the two models is 124%～126% higher compared to the steady scenario.This research provides a theoretical basis for the understanding of LNAPL pollution process in heterogeneous contaminated sites.

Background The rapid development of informatization in primary care in regions inhabited by ethnic minorities during the 13th Five-Year Plan period (2016—2020) , has laid a foundation for the improvement of the serving capacity of primary care supported by information technologies, but there is a lack of research on information technology and the efficiency of primary care. Objective To assess the status including effectiveness of informatization in primary care in China asregions inhabited by ethnic minorities, to explore the impact of information technology on improving the efficiency of primary care. Methods From July to October 2020, 84 primary care institutions in 23 ethnic counties in three autonomous prefectures of Sichuan (Ganzi, Liangshan and Aba) were selected by stratified sampling, and whose directors were invited to complete a self-administered questionnaire survey for understanding the status including effectiveness of informatization in their institutions. Furthermore, the heads of local health committees and directors of primary care institutions in these ethnic counties were invited to attend a group discussion regardingthe setting of departments, service development, status of informatization and settings for information services in primary care institutions. Results In terms of software construction, of the 84 primary care institutions, 57 (67.9%) used the public health system, 44 (52.4%) used the family doctor management system, and 25 (29.8%) use the hospital information system. Forty-seven (56.0%) primary care institutions had achieved vertical connectivity with other institutions (mostly regional- and county-level institutions as well as other members in a regional medical consortium) using information technologies, and regional informatization. Institutions that had horizontal connectivity with 69 (74.4%) of the primary care institutions were medical insurance departments. Only 18 (21.4%) institutions had independent information departments, and 34 (41.0%) did not employ information professionals. Lack of information professionals〔64 (76.2%) 〕, insufficient funds〔52 (61.9%) 〕 and weak infrastructure〔51 (60.7%) 〕 were main problems revealed in informatization construction. Institutions with regional informatization, vertical connectivity with other institutions, and the construction of a hospital information platform with electronic medical record system as the core were superior to those without in terms of setting of laboratory and examination departments and provision of traditional Chinese medicine services (P

Background and purpose: There are various chemotherapy regimens for breast cancer, and multiple chemotherapy drugs are often used in combination. The chemotherapy cycle is long, and chemotherapy drugs cause many adverse reactions. In clinical diagnosis and treatment of tumor patients, liver injury caused by chemotherapy is also a common adverse reaction. Of the drug-induced liver injuries, liver injury caused by antitumor drugs accounts for 15%. We aimed to provide clinical strategies for chemotherapy patients to reduce adverse reactions, increase chemotherapy compliance and improve the quality of life of patients. Methods: We retrospectively analyzed the risk factors of liver injury caused by chemotherapy in patients with breast cancer who were treated in Fudan University Shanghai Cancer Center from January 2017 to December 2017. We enrolled breast cancer patients from Fudan University Shanghai Cancer Center and collected basic information including patient’s information, medical history, chemotherapy, and laboratory indicators related to liver function. According to the presence or absence of liver injury, the patients were divided into case group and control group. Univariate analysis of each factor level and multivariate analysis were used to explore the risk factors of liver injury caused by chemotherapy in patients with breast cancer. The prediction model of liver injury was established by binary logistic analysis, and the predictive ability of the model was tested by receiver operating characteristic (ROC) curve analysis. Results: A total of 724 patients with breast cancer in Fudan University Shanghai Cancer Center were eligible for inclusion in this study. The proportion of patients with liver injury during chemotherapy was 40.74%. The results of logistics analysis based on the results of univariate analysis showed that age, TNM stage, intensive chemotherapy regimen, paclitaxel combined with platinum chemotherapy regimen, anthracycline chemotherapy regimen and chemotherapy cycle became the independent risk factors for liver injury caused by chemotherapy in patients. The accuracy of the binary logistic model: P=1/1+Exp∑(0.901- AX 1 +1.01X 2 +TX 3 -1.82X 4 +5.225X 5 +1.256X 6 + 0.874X 7 -0.764X 8 ), with a specificity of 91.61% and a sensitivity of 81.69%, was 87.60%. The negative predictive value (NPV) was 87.92%, and the positive predictive value (PPV) was 87.00%. At the same time, ROC curve analysis showed that the area under the ROC curve was 0.923 (95% CI: 0.901-0.944, P