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Objective: To evaluate the effects of modification of transferrin on cytotoxicity and intracellular delivery of paclitaxel loaded Poly (lactide-co-glycolide) (PLGA) nanoparticle (NPs)., Methods: PLGA NPs were formulated with microemulsion method, Polyvinyl alcohol (PVA) was used as surfactant (PVA NPs). Transferrin (Tf) was used to modify the NPs (Tf NPs). The cytotoxicity of paclitaxel solution and paclitaxel loaded PVA NPs and Tf NPs were measured in bladder cancer cell line J-82. The intracellular delivery of two kinds of NPs was measured., Results: The half maximal inhibitory concentration (IC50) of paclitaxel loaded PVA NPs and Tf NPs was (44 ± 7) and (49 ± 11) ng/ml respectively and significantly lower than that of paclitaxel solution, which was (81 ± 18) ng/ml (both P < 0.05). The uptake of PVA NPs and Tf NPs by J-82 cells after 2 hours was (89 ± 19) µg/mg cellular protein and (76 ± 16) µg/mg cellular protein. The uptake of two kinds of NPs had no significantly difference. The intracellular level of NPs decreased significantly upon the withdrawal of NPs in medium. However, it became stable 2 hours later and 11.3% PVA NPs and 18.0% Tf NPs remained. The intracellular level of PVA NPs and Tf NPs had no significantly difference at any time point. NPs were distributed in cytoplasm after endocytosis., Conclusions: PLGA NPs can significantly improve the anti-neoplastic effect of paclitaxel on bladder cancer. However, modification of Tf does not change the intracellular dynamics.

Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Traditional Chinese Drug Research & Clinical Pharmacology is the property of Traditional Chinese Drug Research & Clinical Pharmacology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Materiae Compositae Sinica is the property of Acta Materiea Compositae Sinica Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

P7 lipopeptide was synthesized through solidphase peptide synthesis SPPS and hydrophobic-supportassisted liquid-phase peptide synthesis LPPS. Molecular docking technique was used for docking the product with 3OCG protein. MTT assay and Griess reagent were used to detect the biological toxicity and anti-inflammatory activity of P7 lipopeptide. The synthesized P7 lipopeptide was characterized by mass spectrometry. For SPPS and LPPS methods, the yields were 34.45% and 66.31%, respectively; the material consumptions were 129.12 g and 83.08 g, respectively; the waste liquid volumes were 7.12 L and 3.38 L, respectively; PMI were 30.81 and 10.29, respectively; E factors were 1 700.62 and 419.70, respectively. The results of molecular docking showed that the docking binding energy between P7 lipopeptide and p38 protein kinase 3OCG was the smallest. P7 lipopeptide formed hydrogen bonds with residues ARGA70, ARGA189, TYRA35, ARGA67, ASNA155, and ASPA168, and interacted with residues by van der Waals forces, π-alkyl groups, and carbon hydrogen bonds. P7 lipopeptide had no biological toxicity within the concentration of 32 μmol/L. Different concentrations of P7 lipopeptide could significantly downregulating the release of NO in LPS-induced RAW264.7 cell inflammatory model. In summary, the LPPS method had higher yield and lower raw material consumption compared with SPPS method, making it suitable for industrial production. The results of molecular docking and cell experiments indicated that the designed P7 lipopeptide had good anti-inflammatory and antiosteoporosis activities. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Jilin University (Medicine Edition) is the property of Jilin University Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Poly(ethylene glycol) monomethyl ether-b-poly(diisopropylaminoethyl methacrylate-b-poly(methacrylic acid)/lipoic acid (mPEG-b-PDPA- b -PMAA/LA), a novel amphiphilic triblock polymer, was designed and synthesized. A core-crosslinked micelle (CCM) for drug delivery was then formed by mPEG-b-PDPA-b-PMAA/LA through self-assembly and crosslinking reaction. The resulting CCM exhibited high stability in blood serum and against dilution compared to uncrosslinked micelles (UCM). CCM achieved high drug loading compared to UCM through investigating the encapsulation and release kinetics of DOX. With increasing polymerization of PDPA and PMAA, the drug-loading content (DLC) and drug-loading efficiency (DLE) of doxorubicin (DOX) in CCM increased, suggesting that the encapsulation capacity for DOX was enhanced with an increase in the hydrophobic core of the micelles. In addition, the CCM was shown to be capable of controlled release of drugs. An acidic pH and the presence of glutathione (GSH) led to the protonation of PDPA and collapse of PMAA gel, respectively, which caused the collapse of the micellar construct. This enabled a maximal DOX release rate of 90%. Then, the simultaneous loading and release of two therapeutics, DOX and HApt, using the micellar system was investigated. HApt and DOX were sought to be encapsulated in the PMAA gel and PDPA layer, respectively. The dual drug-release behaviors of the system were evaluated under different environmental stimulations. The CCM system for drug delivery designed in this paper is expected to achieve a synergistic effect of gene therapy and chemotherapy. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Prevention & Treatment For Stomatological Diseases is the property of Journal of Prevention & Treatment For Stomatological Diseases Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of China Surfactant Detergent & Cosmetics (2097-2806) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of China Medical University is the property of Journal of China Medical University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Pharmaceutical Practice & Service is the property of Journal of Pharmaceutical Practice Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Pharmaceutical Practice & Service is the property of Journal of Pharmaceutical Practice Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Pharmaceutical Practice & Service is the property of Journal of Pharmaceutical Practice Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Asian Journals of Ecotoxicology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Stable nucleic acid lipid nanoparticles (SNALP) is a new type of nano-preparation prepared by encapsulating nucleic acid drugs in nano-carriers. At present, methods such as membrane extrusion and ethanol injection are commonly used in the laboratory to prepare SNALP, but there are some shortcomings such as poor repeatability, large quality differences between batches and complicated operation. Therefore, a stable, controllable and simple preparation technology of SNALP based on microfluidic technology is developed in this paper, in order to realize the repeatability of SNALP preparation and control the particle size. Compared with the conventional ethanol injection method, the SNALP prepared by this technology has smaller particle size, more uniform distribution, small difference between batches and good stability. The development of this experimental technology provides technical support for the preparation of SNALP with different properties in the future. [ABSTRACT FROM AUTHOR]

Take the 3D reconstruction of underwater targets as the background, this study proposes an experimental design plan for the three-dimensional reconstruction of underwater targets based on monocular vision. First, a monocular camera is used to rotate and shoot an underwater object from multiple angles without the need to pre-calibrate the camera to generate an initial set of image inputs. Then, problems such as a decrease in the number of feature point extractions in the underwater image, an increase in the mismatch ratio due to color distortion, and low contrast ratio, by using the proposed feature matching SIFT HC method, the gray difference between pixels and the color contrast between pixels are minimized by bimodal distribution function to obtain the gray image to be matched. On this basis, Helinger distance is used as similarity measure for feature matching to effectively maintain the original image color contrast, improve the number of effective feature points in adjacent images, and eliminate the false matching point pairs to improve the matching rate of feature points. Finally, the 3D point cloud is reconstructed to effectively restore the surface details of the target object. Experimental results show that the experimental equipment is simple, without pre-calibrate the camera, and can effectively restore the 3D structure and surface details of the target object in view of the color distortion of the underwater image. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Refrigeration Technology is the property of Shanghai Society of Refrigeration and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of East China University of Science & Technology is the property of Journal of East China University of Science & Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Diabetes Mellitus is the property of Chinese Journal of Diabetes Mellitus and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to develop a green and simple method to synthesize NCAs, dimethyl carbonate (DMC) was used to synthesize NCAs instead of phosgene by the "one-pot method". The influence of components on the product was studied, the modes of substrate adsorption and transformation over catalysts were characterized by variable-temperature FTIR and TG-MS-IR, the regulation mechanism of NCAs was precisely synthesized by DMC, and the possible catalytic mechanism was provided. It is shown that when the ratio of alanine to DMC was equal to 1:3 and the reaction was carried out at 150 °C for 9 h in DMF, the highest yield of 53.2% for N-carboxy alanine anhy-dride(Ala-NCA)by the "one-pot method" was obtained. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Functional Polymers is the property of Journal of Functional Polymers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Currently, the first-line drugs for invasive fungal infections (IFI), such as amphotericin B, fluconazole and itraconazole, have drawbacks including poor water solubility, low bioavailability, and severe side effects. Using drug delivery systems is a promising strategy to improve the efficacy and safety of traditional antifungal therapy. Synthetic and biomimetic carriers have greatly facilitated the development of targeted delivery systems for antifungal drugs. Synthetic carrier drug delivery systems, such as liposomes, nanoparticles, polymer micelles, and microspheres, can improve the physicochemical properties of antifungal drugs, prolong their circulation time, enhance targeting capabilities, and reduce toxic side effects. Cell membrane biomimetic drug delivery systems, such as macrophage or red blood cell membrane-coated drug delivery systems, retain the membrane structure of somatic cells and confer various biological functions and specific targeting abilities to the loaded antifungal drugs, exhibiting better biocompatibility and lower toxicity. This article reviews the development of antifungal drug delivery systems and their application in the treatment of IFI, and also discusses the prospects of novel biomimetic carriers in antifungal drug delivery.

To break the main obstacle that the cell wall preventing delivery of exogenous biomolecules into intact cell during plant genetic transformation, nanomaterial-mediated gene delivery technology was currently successfully applied. Traditional transgenic methods require the aid of external force or the mediation by creatures like agrobacterium. The nano-carriers for genetic delivery possess outstanding advantages such as excellent biocompatibility, ability to protect external DNA/RNA against nuclease degradation, high transfection efficiency, thus provide a new promising way for plant genetic engineering. In this paper, we summarized the bottlenecks of plant genetic transformation met currently, the drawbacks of the existing methods and the advantages of nanomaterial-mediated delivery. And the key techniques and research progresses of nanoparticles-mediated plant transformation were discussed in detail. Ultimately, the advantages and disadvantages as well as future direction and problems of this newly formed technology were also discussed and prospected. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Guangdong University of Technology is the property of Journal of Guangdong University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Functional Polymers is the property of Journal of Functional Polymers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)