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Objective To investigate the effect of endoplasmic reticulum stress (ER stress) in cisplatin-induced apoptosis of human cervical cancer HeLa cells. Methods HeLa cells were used as the study object which were divided into four groups: TUNI (5mg/L) group, cisplatin (6mg/L) group, TUNI(5mg/L)+cisplatin(6mg/L) group, and negative control group (no drug treatment). MTT assay was employed to examine the growth status of the cells. Hoechst staining was used to observe the morphological change in the nucleus. Immunoblotting was used to detect the activation of apoptotic proteins, caspase-3 and caspase-4. Indirect immunofluorescence was used to assess the expression of the protein disulfide isomerase (PDI) and phosphorylated histone H2AX (γ-H2AX). Results MTT assay showed that the growth inhibition rates were 2.65%±2.71%, 19.60%±4.34%, 44.69%±7.07% and 0% in TUNI group, cisplatin group, TUNI+cisplatin group and control group, respectively (P

Objective To investigate the effect of Huntingtin-interacting protein1 (HIP1) gene silencing on the proliferation of PC-3 cells in a human androgen-independent prostate tumor. Methods pSlience-shHIP1, a shRNA expression vector targeting HIP1, was constructed and transfected into PC-3 cells by liposome. RT-PCR was adopted to detect the silencing effect of HIP1 gene. Western bloting was then used to confirm the effective target point. Moreover, the scratch assay and growth curve assay were performed to analyze the effect of HIP1 on the proliferation of PC-3 cells. Results The efficiency of HIP1 gene silencing was increased to 83% (PConclusions The pSilence-HIP1 expression vector can specifically suppress the expression of the HIP1 gene. In addition, HIP1 gene silencing can inhibit the proliferation and migration of PC-3 cells.