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Background and purpose: Expression level of histone deacetylase 5 (HDAC5) is abnormally high in breast cancer, and HDAC5 synergically promotes proliferation and migration of breast cancer cells by cross-talking with LSD1. In this study, the effects of sulforaphane (SFN) on invasion, migration and epithelial-mesenchymal transition (EMT) of mouse breast cancer 4T1 cells were evaluated, and the role of HDAC5 in the treatment with SFN was observed. Methods: The effects of SFN on proliferation of 4T1 cells was analyzed by cell counting kit-8 (CCK-8) method. The cytotoxicity of SFN was detected by lactate dehydrogenase release assay. 4T1 cells were transfected with pcDNA3.1(+)-flag-HDAC5 plasmid, and the single cell clone was obtained by G418 screening. Then the monoclonal cell lines with stable high expression of HDAC5 were detected and identified using Western blot. The effects of HDAC5 overexpression and SFN treatment on the migration and invasion of 4T1 cells were analyzed with scratch test and transwell method. The effects of SFN on expressions of EMT markers E-cadherin, N-cadherin, vimentin and matrix metalloproteinase-9 (MMP-9) in 4T1 cells were tested using Western blot. The effect of SFN treatment on proliferation and metastasis in xenograft tumor model of 4T1 cells in vivo and the relationship between SFN and HDAC5 expression were observed in BALB/c mice. The Ki-67 proliferative index in xenograft tumor and lung metastatic tumor tissues was detected using immunohistochemical analysis. The heart, liver and kidney tissues of tumor-bearing mice were taken and detected using H-E staining, therefore to evaluate the toxic effects of SFN on tumor-bearing mice. Results: SFN inhibited the proliferation (P

With the development of sensor technology and computer technology, equipment can produce a large amount of data during the development and production process. These data are massive, multi-source, and heterogeneous. Enterprises need to consider how to effectively manage large amount of equipment data and use processed data to enhance manufacturing capabilities. This paper studies the data of typical equipment, such as satellite, airplane, etc., and proposes a distributed data storage method based on a pre-partitioning strategy. Through studying the pre-partitioning mechanism of HBase and the characteristics of equipment data, this paper studies impact factors for rapid storage of equipment data, and proposes a fast storage algorithm, which can store large amount of data into HBase in a balanced and fast manner. Finally, this paper evaluates the data storage performance, load balance, and applicability of various types of equipment. The experimental results show that this method can be applied to many types of equipment and has good performance in data storage efficiency.

It introduces the application of the regeneration technology for deteriorated sulfolane in aromat-ics extraction unit. The technology has successfully solved the problems of low pH value of sulfoxide solvent, high chloride content and mechanical impurity plugging equipment. With the help of the technology, the pH value of the solvent is above 7. 5, the concentration of chloridion down from 64 mg/L to 10 mg/L, and the mechanical impurities in sulfolane is filtered. During the operation of the unit, it found this unit can't be used with monoethanolamine at the same time. The skills and precautions in the resin transformation process are summarized, which is of great significance to guide the production. [ABSTRACT FROM AUTHOR]

Objective: Acute respiratory distress syndrome (ARDS) is considered to be a pulmonary manifestation of systemic inflammatory response syndrome (SIRS), often occurring as a complication of disease, and worsening the prognosis of patients. In recent years, the incidence of trauma has increased year by year. Severe trauma can lead to SIRS, which is one of the common risk factors of ARDS. The spleen is the largest peripheral immune organ of the body, containing a large number of immune cells and secreting inflammatory factors. The inflammatory factors play an important role in the formation of traumatic ARDS. In recent years, the benefits of treating ARDS by inhibiting spleen-induced inflammatory response have gradually been discovered, providing new ideas for the treatment of ARDS. Therefore, the research status of spleen-mediated inflammatory response in traumatic ARDS is of great significance for the prevention and treatment of traumatic ARDS. This article reports the spleen-mediated systemic inflammatory response, the role of inflammatory mediators in the development of ARDS, and the current state of research on ARDS treatment to explore new approaches to the prevention and treatment of traumatic ARDS.