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Objective: A process was developed for the purification of bran polyphenols from HPD 600 macroporous resin. The purity and antioxidant activity of the extracts were analyzed before and after purification, and the polyphenol fractions in the purified products were characterized. Methods: The bound polyphenols in wheat bran were extracted by alkali hydrolysis, and the dynamic adsorption-desorption conditions of the macroporous resin were optimized by single-factor test to determine the optimal purification process. The antioxidant activity fraction was determined by ABTS and DPPH radical scavenging rate. The characterization of the polyphenol fraction in the purified product was completed by UPLC-MS/MS. Results: Under optimal process conditions, the purity of the polyphenols and the antioxidant activity of the purified product were significantly increased. The purified products contained mainly nine polyphenolic compounds, in order of retention time: p-hydroxybenzoic acid, caffeic acid, vanillin, p-coumaric acid, trans-ferulic acid and salicylic acid. Conclusion: The macroporous resin purification process is effective in enriching bran polyphenols and the resulting purified product has a significantly higher antioxidant capacity, while maintaining the richness of the polyphenolic species.

[ Objective ]The cell nucleus morphological changes of peanut root tip under aluminum stress were studied in order to provide scientific references for programmed cell death in peanut root tip cells induced by aluminum. And it was verified that Steedman' s wax sectioning and DAPI dyeing techniques could be used to observe nucleus morphology. [ Method ]Two varieties of peanut (ZH2 Al-sensitive and 99-1507 Al-tolerant) root tips were treat - ed with 100 μmol/L AlCl3 for different time (0, 4, 8, 12 h). Nucleus morphological changes were observed by Steedman 's wax sectioning and DAPI dyeing. [ Result ] 100 μmol/L AlCl3 could induce morphological changes of peanut root tip cell nucleus, such as nuclear chromatin condensation, chromatin margination and nuclear crescent shape. These were obvious characteristics of programmed cell death. PCD occurrence was negatively related to aluminum tolerance and positively correlated with the duration of treatment time in peanut. [ Conclusion ] Steedman' s wax sectioning and DAPI dyeing is a rapid and efficient method for the observation of plant nucleus morphology. [ABSTRACT FROM AUTHOR]

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Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)