1. [Changes of amino acids neurotransmitters in striatum of hemi-parkinsonian rhesus monkey after high frequency stimulation of subthalamic nucleus].
- Author
-
Zhao XD, Zhou XP, Liu HH, Li BM, Hu XW, Li FQ, and You BM
- Subjects
- 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Animals, Chromatography, High Pressure Liquid, Disease Models, Animal, Glutamic Acid metabolism, Macaca mulatta, Microdialysis, Neurotoxins toxicity, Parkinson Disease, Secondary chemically induced, Parkinson Disease, Secondary metabolism, Subthalamic Nucleus, Taurine metabolism, gamma-Aminobutyric Acid metabolism, Amino Acids metabolism, Corpus Striatum metabolism, Deep Brain Stimulation methods, Neurotransmitter Agents metabolism, Parkinson Disease, Secondary therapy
- Abstract
Objective: To investigate the influence of high frequency stimulation of the subthalamic nucleus on the levels of amino acids neurotransmitters in striatum of hemi-parkinsonian monkeys., Methods: Two rhesus monkeys were successfully prepared for the subsequent microdialysis sessions. Collecting the dialysate before turning on the pulse generator, and collecting at 1 week, 1, 8 and 12 months after high frequency stimulation of the subthalamic nucleus. The level of Glu, GABA and Tau were determined by high performance liquid chromatography and fluorometric detection (HPLC-FD)., Results: After high frequency stimulation (HFS), PD symptoms of monkeys significantly improved. The levels of Glu in putamen and caudate nucleus of electrodes side at 1 week, 1, 8 and 12 months were increased significantly. The levels of GABA in putamen and caudate nucleus of electrodes side at 1 week, 1 month increased significantly compared with before turning on the pulse generator while decreased at 8, 12 months. The level of Tau in putamen and caudate nucleus increased significantly., Conclusion: Long-term STN HFS can increase the level of glutamate and taurine, while decrease the level of GABA in putamen and caudate nucleus of electrodes side. It improves symptoms of hemi-parkinsonian rhesus monkey significantly.
- Published
- 2007