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Wernekink commissure syndrome (WCS) is very rare. Four patients with WCS, admitted to our hospital from April to May 2018, were chosen for this study, and their clinical manifestations, imaging features, and etiology were retrospectively analyzed based on the literatures. All patients with WCS manifested as bilateral cerebellar ataxia such as symmetrical limb and trunk ataxia, but the degree of ataxia was asymmetrical distribution based on the anatomy. Dysarthria was the main and constant clinical manifestation of the syndrome. Ophthalmoplegia had great variability, and WCS with oculomotor nerve palsy may be considered as atypical WCS. The incidence of olivary degeneration and palatine myoclonus is relatively low, which may be related to the difference in the reported time intervals of cases. Changes in consciousness and emotion may be the characteristic of neglected WCS, which should be paid more attention. Cerebral infarction is the main etiology of WCS. We reported that cerebral infarction and WCS was the first symptom in a patient with systemic lupus erythematosus (SLE). We should pay more attention to special etiology in diagnosis and treatment of WCS.

Objective: To study the effect of fatty acids composition on swarming mobility in Pseudomonas aeruginosa., Methods: We constructed a fabF-knockout mutant of PAO1 (YFF-1) by double exchange principle, overexpressed FabF in YFF-1 mutant to recover the mobility, and compared the swarming ability of wild type, YFF-1 mutant and mutant with plasmid pUCP18Gm-fabF. The change of fatty acids composition was analyzed using gas chromatography to explain the difference of swarming ability., Results: Swarming ability disappeared in YFF-1 mutant and was recovered in YFF-1 with plasmid pUCP18Gm-fabF. Gas chromatography analysis revealed that fatty acids composition changed in YFF-1. The cis-vaccinate acid (C18:1delta11) content decreased from 33.6% to 8.9%, and the ratio of unsaturated fatty acids to saturated fatty acids (UFA: SFA) was deduced from 0.96 to 0.74. The recovery of cis-vaccinate acid content was 20.9% and UFA:SFA 1.09 after expression of fabF., Conclusion: Expression level of FabF played an important role in regulating swarming ability of PAO1. The decrease of cis-vaccinate acid content and unsaturation degree of fatty acids, especially the sharp decrease of cis-vaccinate acid, may be vital causes of swarming ability disappearance in YFF-1.

Objective: To investigate the role of histone acetylation in regulating influenza virus replicative intermediate double-stranded RNA (dsRNA)-induced interleukin-6 (IL-6) expression in A549 cells., Methods: A549 cells were treated with influenza virus replicative intermediate dsRNA, histone deacetylase (HDAC) inhibitor trichostatin A (TSA), or HADC small interfering RNA (siRNA). The changes in the cellular IL-6 promoter activities were detected by dual-luciferase assay, and IL-6 mRNA and protein expressions in the cells were determined using real-time RT-PCR and ELISA, respectively., Results: Influenza virus replicative intermediate dsRNA obviously up-regulated IL-6 expression in the cells. HDAC inhibitor TSA significantly enhanced the activity of IL-6 promoter and increased IL-6 mRNA expression in A549 cells, and HDAC3 may play an important role in this process. HDAC inhibitor TSA and DNMT inhibitor DAC showed no synergic effect in regulating IL-6 expression., Conclusions: Influenza virus replicative intermediate dsRNA-induced IL-6 expression in A549 cells is regulated by histone acetylation.