Search

Your search keyword '"Hong Bo Li"' showing total 2 results
Start Over Author "Hong Bo Li" Language chinese
2 results on '"Hong Bo Li"'

1. Fast 3D Pose Estimation Method for High Speed GMAW of Large Box Girders

Author

LI Gaoyang, JIA Aiting, HONG Bo, LI Xiangwen, GAO Jiapeng

Subjects
large box girder, gas metal arc welding (gmaw), 3d pose estimation, point cloud segmentation, Engineering (General). Civil engineering (General), TA1-2040, Chemical engineering, TP155-156, Naval architecture. Shipbuilding. Marine engineering, VM1-989
Abstract

In the process of high speed gas metal arc welding (GMAW), large box girder has many complex working conditions, such as positioning weld, low assembly accuracy, difficulty in strictly ensuring the pose of workpiece through tooling, real-time change of 3D pose of weld, etc. The vision based weld recognition method has a large amount of calculation and is not aimed at the workpiece with positioning weld, making it difficult to obtain the 3D pose of large box girders quickly. Aimed at this problem, a fast 3D pose estimation method for large box girder based on laser displacement sensing and point cloud clustering was proposed. Using this method, the vertical plane and flat plane of the welding seam of large box girders were obtained by fast segmentation of point cloud. Then, the pose information of welding seam was calculated. A pose information estimation test was conducted for welds with different poses. The results show that when the welding speed is up to 1200 mm/min, the pose error of the weld is less than 0.25 mm and 1.8°respectively. The robustness of the automatic welding of large box girders to complex conditions of positioning weld and low assembly accuracy is enhanced, which greatly improves the welding quality.

Published
2022
Full Text
View/download PDF

2. Effect of HSP27 on heat stress-induced oxidative stress injury and apoptosis in F98 cells

Author

Hong-bo LI, Lei SU, Xin-feng LIN, Min-yong WEN, Feng-li ZHAO, Si-hui WU, and Lin WANG

Subjects
heat stress, reactive oxygen species, endocrine system, lcsh:R5-920, animal structures, mitogen-activated protein kinases, heat shock protein 27, lcsh:R, apoptosis, lcsh:Medicine, lcsh:Medicine (General)
Abstract

Objective To explore the effect of HSP27 on heat stress-induced oxidative stress injury and apoptosis in F98 cells. Methods The F98 cells were stress at 43 degrees for 60 minutes. After the stress, cells were collected at different time points (0, 3, 6, 9, 12h), the phosphorylation levels of HSP27 at various sites were examined by Western blotting. To explore the function of mitogen-activated protein kinase (MAPKs) pathway on HSP27 phosphorylation, MAPK inhibitors (SB203580, SP600125, PD98059) and adenoviruses expressing MAPK-activated protein kinase 2 (MK2) were employed. To decipher the mechanism of HSP27 phosphorylation on cell apoptosis, adenoviruses carrying HSP27 phosphorylation resistant mutations were transduced into the cell. Then, cell viability test kit, reactive oxygen species (ROS) test kit, apoptosis test kit, and caspase-3 activity test kit were used to detect the cell viability, ROS activities, and apoptosis of F98 cells. Results After heat stress, the expression of HSP27 increased slightly and its three Serine sites were all phosphorylated, with Ser78 being the main phosphorylation site. After pretreatment with MAPKs inhibitors, cells pretreated with SB203580 showed the lowest level of phosphorylation on HSP27 phospho-sites (P0.05). Conclusion The p38MAPK/MK2 pathway regulates the apoptosis of F98 cells through the phosphorylation of HSP27 Ser78 site. DOI: 10.11855/j.issn.0577-7402.2018.10.08

Published
2018

Catalog

Books, media, physical & digital resources
See catalog results