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Contactless sample manipulation is attracting great attention in a wide range of fields, including analytical chemistry, biological science, and food industry. A better understanding on the droplet dynamics in acoustic levitation is vital to realize the lab-in-a-drop technology. Here, this paper reviews the recent research progress on the contactless droplet manipulation driven by acoustic levitation, focusing on droplet levitation, transport, coalescence, mixing, and evaporation dynamics are presented and discussed. [ABSTRACT FROM AUTHOR]