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Currently, the design of coal mine roadway support schemes is still mainly based on manual design, engineering analogy, and FLAC model simulation, which has problems such as strong subjectivity, low universality, and insufficient utilization of coal mine support big data. The design method based on expert systems has cumbersome rule setting procedures, large engineering quantities, and low intelligence. Case based reasoning (CBR) and deep learning techniques are introduced into the field of roadway support scheme design. Based on text big data such as coal mine support regulations, support standards, and coal mine roadway geological reports, an intelligent design method for coal mine roadway support scheme is proposed. The method obtains 346 sets of roadway support data from different coal mines, extracts structured data and divides it into input and output parameters, and optimizes the input and output parameters through constant attribute variable filtering and high correlation filtering methods. The method establishes a CBR model and imports the extracted structured data into the CBR model to form a case library of support scheme comparison and selection. The method calculates the similarity between the new roadway support scheme and the historical scheme, and outputs the three historical schemes with the highest similarity for comparison, achieving similar case comparison. BP neural network and long short term memory (LSTM) network are respectively used to establish automatic generation models for coal mine roadway support schemes. By comparing the prediction indicators, it is determined to use the combination of LSTM model and CBR model to establish an intelligent design system for coal mine roadway support scheme. The system is used for the design of auxiliary transportation roadway support scheme in the F6226 working face of Buliangou Coal Mine excavation. Through experiments, it is verified that the deformation of the two sides of the roadway and the maximum displacement of the roof under the system generated scheme are smaller than those under the manual design scheme. The integrity of the roadway roof and two sides is good, the bearing capacity of the surrounding rock is enhanced, and the support effect is significant.

Background The incidence, mortality and disability rates of ischemic stroke are high, and the reperfusion injury after thrombolytic therapy has a great impact on patients. Acupuncture is a characteristic therapy for the treatment of the disease, but the action mechanism remains unclear. Objective To observe the effect of Tongdu Tiaoshen electroacupuncture (EA) pretreatment on miR-124-3p/glycogen synthase kinase β (GSK-3β) /cyclin D (Cyp-D) signaling pathway and mitochondrial permeability transition pore (MPTP) of cerebral ischemia-reperfusion injury (CIRI) rats, and explore its possible mechanism of prevention and control of CIRI. Methods From June to August 2022, a total of 100 clean SD rats were randomly divided into the sham operation group, model group, EA group, agonist group and EA + inhibitor group, with 20 rats in each group. For 7 d of intervention before modeling, in the EA group and EA + inhibitor group, "Baihui" (GV 20) , "Fengfu" (GV 16) and "Dazhui" (GV 14) were selected to perform electroacupuncture 1 time a day for 7 days. For 24 h before modeling, miR-124 agonist and inhibitor (5 nmol) were injected into the lateral ventricles in the EA + agonist group and inhibitor group. Except for the sham operation group, the right cerebral ischemia-reperfusion model of rats was prepared by the modified suture method in the rest groups. The right cerebral cortex of rats was taken, the degree of neurological impairment in each group was observed using mNSS scale and TTC staining, the nerve cell injury was observed by TUNEL staining and transmission electron microscopy. The expressions of GSK-3β, p-GSK-3β, Cyp-D, Cyt-C and Caspase-3 in cerebral cortex of each group were detected by immunofluorescence staining, Western blot and real-time quantitative PCR. The degree of MPTP openness was detected by flow cytometry, the higher rate of MPTP-positive cells indicated greater degress of MPTP openness. Results Except for the sham operation group, all rest groups of rats were successfully modeled. Compared with the sham operation group, mNSS score and infarct volume of brain tissue were increased, mitochondrial structure was seriously damaged, cell apoptosis index was increased, p-GSK-3β positive expression was reduced, Cyp-D positive expression was enhanced, miR-124-3p, Cyp-D, Cyt-C and Caspase-3 mRNA expressions were increased, p-GSK-3β/ GSK-3β ratio was decreased, relative Cyp-D, Cyt-C, and Caspase-3 protein expressions were increased, and MPTP openness degree was increased in the model group (P

Objective To explore the efficacy of wrapping the hepatic artery with detached ligamentum teres hepatis during pancreaticoduodenectomy (PD) on preventing postoperative intra-abdominal hemorrhage. Methods A total of 186 patients who received PD in the First Affiliated Hospital of Xinjiang Medical University from June 2020 to June 2022 were divided into wrapping group (n=86) and non-wrapping group (n=100) according to whether the detached ligamentum teres hepatis was used to wrap the hepatic artery during the operation. The clinical data on perioperative and postoperative complications were collected and retrospectively analyzed in two groups. Results PD was successfully performed and completed in both groups, and there was no significant difference in age, gender, BMI, length of hospital stay, operative time and intra-operative blood loss between two groups (P＞0.05) . The incidences of pancreatic fistula, biliary fistula and abdominal infection were similar in two groups (P＞0.05) , but the incidence of postoperative bleeding in wrapping group was significantly lower than that in non-wrapping group (1.16% vs 9.00%, P＜0.05) . Logistic regression analysis showed that the pancreatic and biliary fistulas of grade B/C were the independent risk factors for postoperative abdominal bleeding after PD (P＜0.05) , and the wrapping of the hepatic artery with the detached hepatic round ligament during operation was an independent protective factor for postoperative abdominal bleeding after PD (P＜0.05) . Conclusion Wrapping the hepatic artery with detached ligamentum teres hepatis during PD surgery may reduce the incidence of postoperative abdominal bleeding and is simple, safe and feasible to perform without additional complications．

Objective To describe the occurrence of general surgical complications and establish prediction models for different types of complications based on a multicenter cohort study. Methods Based on Modern Surgery and Anesthesia Safety Management System Construction and Promotion(MSCP), patients who underwent general surgery in 4 hospitals from January to June 2015 and from January to June 2016 were selected as participants, and perioperative data of patients were collected. Logistic regression was used to identify risk factors and predict complications. Results Among 19 223 patients, 830(4.32%) had complications. Among participants who had complications, 371(44.70%) had incision complications, 190(22.89%) had fistula complications, 310(37.35%) had infection complications, 161(19.40%) had failure complications, and 104(12.5%) died. There were significant differences in the risk factors of different types of complications. The risk factors of incision,infection and failure covered the whole perioperative period, while fistula complications mainly focused on the difficulty of surgery and postoperative treatment, and for death outcomes, postoperative risk factors were more severe than preoperative risk factors. The areas under the curves of prediction were between 0.80-0.93. Conclusions In general surgery, different types of complications have different risk factors. The targeted prediction model can avoid the rough simplification of complications and fuzzy influence of each factor, and can provide reference for the prevention of complications.

In terms of the fatigue fracture problems of Zr-Mo titanium alloy (0.6Zr3Mo) oil well pipe caused by working environment during service, the fatigue properties of titanium alloy under different concentrations of hydrofluoric acid and laboratory conditions were investigated and compared by stress loading. Results showed that under the action of 70%σs stress amplitude, titanium alloys exhibited cyclic softening characteristics in both corrosive and non-corrosive environments. The hydrofluoric acid solution promoted the nucleation of fatigue cracks in titanium alloys and accelerated the propagation rate of fatigue cracks, which shortened the fatigue life of titanium alloys, and the fatigue life showed a decreasing trend with the increase of corrosion solution concentration. Meanwhile, the concentration of hydrofluoric acid had a certain impact on the crack propagation mode of titanium alloys, and in laboratory environments, the crack propagation of titanium alloys was mainly characterized by periodic cleavage. In low concentration hydrofluoric acid environments, secondary cracks formed by hydrofluoric acid corrosion could easily induce the formation of cleavage surfaces, and the crack propagation still propagated in a cleavage manner. In high concentration hydrofluoric acid environments, the propagation mode of cracks had changed with an increase in the degree of crack propagation in the ductile zone and a decrease in the degree of crack propagation in the form of cleavage.

The excessive discharge of phosphorus-containing substances will lead to eutrophication of water bodies and cause water environmental pollution.Hence，the prevention and control of phosphorus pollution is one of the major issues in China ecological environment construction.In this paper，the treatment methods for removing phosphorus from phosphorus-containing wastewater such as chemical precipitation method，adsorption method，ion exchange method，membrane separation method and biological method were emphatically expounded，and the advantages and disadvantages of each method were compared and analyzed.Moreover，based on the concept of cleaner production，the countermeasures for the prevention and control of phosphorus-containing wastewater in the surface treatment industry were put forward from the three aspects of source control，phosphorus recycling，and automatic control.

The clinical symptoms and signs of methamphetamine-associated psychosis （MAP） and schizophrenia are highly similar, but the situation is completely different when MAP and schizophrenia patients need to be assessed for criminal responsibility after they comitted a harmful behavior. Therefore, the distinction between the two psychoses is very important in forensic psychiatry. At present, the identification of these two psychoses is mainly dependent on the corresponding criteria such as the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition （DSM-5） and the Chinese Classification of Mental Disorders Version 3 （CCMD-3）. It’s challenging to diagnose and distinguish between the two in practical cases due to their similar clinical symptoms and the lack of effective objective indexes. Different from the limitations of single omics, integrative omics intergrates data from multiple dimensions and has been extensively studied in the field of schizophrenia and has achieved some preliminary results. In view of the correlation between MAP and schizophrenia and the potential application value of integrative omics, this paper proposes an integrative omics strategy for MAP pathogenesis and forensic identification, aiming to improve the further understanding of the relationship between the two psychoses and the corresponding pathogenesis. It also provides references for the future exploration of integrative omics in forensic precise identification and effective monitoring and early warning methods.

In order to investigate the effect of nano-modified ethylene-vinyl acetate （MEVA） on physical and rheological properties of styrene-butadiene rubber （SBR） modified asphalt, MEVA/SBR composites modified asphalt with different proportion of MEVA were prepared by blending method. The basic physical properties of the composite modified asphalt were tested, and rheological characteristics was analyzed by using temperature scanning of dynamic shear rheometer. The high and low temperature performance and traffic load grade that the composite modified asphalt could withstand were evaluated by multiple stress creep recovery test and ben-ding beam rheometer. The results showed that the addition of MEVA could effectively improve the physical and rheological properties of SBR modified asphalt, and the modification effect gradually increased with the increase of MEVA amount. Consi-dering high and low temperature properties, the re-commended amount of MEVA was mass fraction of 5%. Compared with SBR modified asphalt, the high temperature grade of MEVA/SBR composite modified asphalt with the best mixing ratio was increased by 18 ℃, and it could bear traffic load of “S” grade at 76 ℃, and its low temperature performance could meet the service temperature grade requirements of -28 ℃.

An inductively coupled plasma atomic emission spectrometry (ICP - AES) method was developed for the determination of 12 impurity elements (Cd, Mn, Cr, Cu, Fe, Ca, Zn, Al, Co, Nd, Pr and Ce) in nickel plating solution for Nd - Fe - B. The optimum working parameters of the instrument and the analytical spectral lines of each element were determined. By using matrix matching and simple bath dilution, the spectral interference to the elements to be detected caused by a large number of matrix substances in the bath was eliminated. Results showed that the linear relationship of these 12 elements was good, the linear correlation coefficient R > 0.999, the detection limit was 0.005～0.690 μg/mL, the results of experiments of precision and recovery showed that this method had the advantages of simple, rapid and accurate, meeting the requirements of daily detection of impurity elements in Nd - Fe - B nickel plating solution.

Rice germ is rich in a variety of nutrients, but at present, most of that is used as a by-product of rice processing for feed or wasted by discarding. The degree of deep processing and utilization is not high, and the economic benefit is low. Through the study about basic nutrients of rice germ, the results showed that there were a lot of proteins composed of abundant kinds of amino acids with high contents of proline, serine, aspartic acid, arginine and leucine. The contents of Mn, Fe and Zn were significantly higher than that of milled rice. The content of fatty acid was higher than that of wheat germ and corn germ, and the proportion of unsaturated fatty acid was higher, so the nutritional quality was better with higher development and utilization value. X-ray diffraction results showed that germ starch was a typical type A starch, with high amylopectin/amylopectin ratio and good digestibility. Through the determination of the nutritional components of rice germ and the comparative study with the common staple food on the market, we can further understand its nutritional characteristics and provide scientific reference for the development and utilization of rice germ.

The flat design of security and stability control system (SSCS) can realize the information interaction between each control sub-station and each sub-grid,which is conducive to reducing the number of SSCS layers and improving its reliability,thus increasing the difficulty of searching the control paths and differentiating the vulnerable components. The bidirectional/loop communication channels are added to the tree-shape SSCS to reduce the intermediate stations and find all the paths from the top to bottom stations based on the adjacent matrix. Considering availability of components and controllable capacity of underlying stations,the control capability of the flat SSCS is newly quantified. Average control ability and average associated path number are defined to evaluate the influence of flattening on the control ability of each station. Analytical sensitivity model of the control capability of flat SSCS with respect to the components' availability is proposed to determine the vulnerable components. The numerical analysis shows that the flat design reduces levels and intermediate stations,and improves the controllability of the SSCS. The impact on the controllability of the SSCS increases by the number of paths and controllable power,but decreases by the number of layers.

Calcium homeostasis of cardiomyocytes depends on the regulation of calcium(Ca2+) transportation through plasma membrane, sarco/endoplasmic reticulum(SR) and mitochondria. One of the main characteristics of heart failure is calcium homeostasis, including cytosolic calcium overload and SR calcium leakage, which leads to transient decrease of myocardial calcium and decrease of contractility.

This paper performed a retrospective analysis of 19 patients diagnosed with pathologically-verified WHO Ⅱ-Ⅲ grade glioma with T2/FLAIR mismatch sign. We extracted the overall characteristics of the tumor area and the image parameters of the mismatch regions from the patients' imaging data for quantitative analysis. Afterward, we investigated how T2/FLAIR mismatch sign affected the diagnostic efficacy for isocitrate dehydrogenase (IDH)-mutant and 1p/19q non-codeleted (IDHMUT/1p/19q+) lower-grade gliomas (LGG). This study showed that the overall and partial T2/FLAIR mismatch sign can be used as an imaging marker to predict IDHMUT/1p/19q+ LGG. The overall imaging characteristics of the tumor area combined with the quantitative parameters of the mismatch regions can improve the diagnostic efficacy for IDHMUT/1p/19q+ LGG.

COVID-19 highlights the lack of interdisciplinary medical talents. The international history of medical education shows the urgent need of high-level interdisciplinary medical talents. Anchoring the goal to develop a global center of talents and highland of innovation, this article takes medical education of Zhejiang University as an example, focusing on and exploring the training mode of high-level interdisciplinary medical talents in the new era. It includes: firstly, optimizing the training mode of eight-year program for medical doctors with non-medical bachelor degree followed with complete education for a medical doctorate that innovates the curriculum system of clinical medicine; secondly, creating the training system of postdoctor of clinical medicine and integrating medical resources that include high-quality talents and health care system, in order to build a high-quality teaching staff with a interdisciplinarity background and innovative bases. It not only strengthens the residents' competency and frontier creativity, but also ensures the sustainable development of interdisciplinary medical talents. The reform of training mode, curriculum system, teaching staff and clinical teaching bases all contribute to the goal of building a country with interdisciplinary talents that serve the frontier of science and technology in the world, the major needs of the country and people's health in the new era.

In order to develop new anti-mold agents and reduce the effect of mildew on the quality and safety during storage, transportation, and circulation of cereals, oils and foods, microorganisms with strong anti-mold activity were screened in this study. Bacteriostasis method was applied and Fusarium graminearum was taken as indicator. Morphological observation, biochemical characterization and 16S rDNA gene sequences alignment were used to identify the strains. The anti-mold bioactive substances were analyzed in different temperatures, pH, and proteinase K treatment. After primary screening, 33 strains with strong inhibitory activity against F. graminearum were obtained. Seven strains were selected after secondary screening and classified as Bacillus. Through the analysis of bioactive substances, the anti-mold effects of the seven strains were not of high temperature and strong acid or base resistant, or obviously influenced by proteinase K treatment. It indicated that the bioactive substances were peptides. Finally, the strain ASAG 62 had good inhibitory effect on common molds (Penicillium flavum, Aspergillus Niger, Aspergillus ochre, Aspergillus flavus and F. graminearum).

Twelve endophytic fungus strain from artemisia sacrorum ledeb were isolated and purified with the method of surface disinfection and tip mycelium selection. The activity of eight strains of endophytic fermentation broth against wheat scab were studied by mycelial growth rate method. Most active strain were identified as Fusarium Redolens by the combination of morphology and ITS rDNA gene sequence. A mass spectrometry method was established to analyze the main metabolites. The results showed that the endophytic fungus (No: 20202707) from artemisia sacrorum ledeb demonstrated the strongest inhibitory effect on Fusarium graminearum, and the inhibitory rate was 65.27%. Its main metabolites were 3, 4-dihydrocoumarin, 5′deoxyribonuc leoside, Harmala alkaloid, benzofuran and benzothiazole.

Objective To explore the role of IDH1 in the expression of RNA binding proteins (RBPs) in glioma cell line LN229. Methods Lentivirus was used to construct IDH1 knockdown and control cells, and the expression of IDH1 was validated by RT-qPCR (real-time quantitative PCR) and Western blot. Then the total RNA of cells from each group was extracted for RNA sequencing (RNA-seq); the differentially expressed genes (DEGs) were identified by limma package and enriched pathways were analyzed; the differentially expressed RBPs were selected according to the RBP database and protein-protein interaction network was constructed by string database; finally, the expression level of candidate RBPs was tested in IDH1 knockdown, over-expressed and mutant cells(R132H) respectively. Results A total of 259 DEGs were detected in IDH1 knockdown LN229 cells, including 146 upregulated and 113 downregulated genes. These DEGs were significantly enriched in interferon related pathways and included 14 RBP-encoding genes, 9 of which were significantly enriched in interferon related pathways and showed close interaction; compared with the control group, IDH1 knockdown in LN229 significantly upregulated the expression of all the nine RBPs (P＜0.001), and IDH1 overexpression only downregulated IFIT3 (P＜0.01) involving in interferon pathway, while IDH1 mutation had no significant effect on IFIT3. Conclusions IDH1 regulates expression of interferon pathway related gene IFIT3 in LN229 cells.

Objective To investigate the diagnosis, treatment and prognosis of lymphoepithelial carcinoma of the parotid gland. Methods Data from 22 patients with parotid lymphoepithelial carcinoma from 2012 to 2019 were collected, and their clinical manifestations, imaging examinations, treatment methods and follow-up results were retrospectively analyzed. Results Among the 22 patients, 8 were males and 14 were females. The patients ranged from 26 to 61 years old, with a complaint duration ranging from 3 days to 18 years. One patient had multiple unilateral lumps in the parotid gland, and the other 21 patients had single unilateral lumps in the parotid gland. All patients underwent preoperative spiral CT examination. CT showed a soft tissue lump in the parotid tissue, the internal density shadow was not uniform, the CT value ranged from 26 to 81 Hu, and 15 patients presented elliptical lesions with clear boundaries. Seven patients presented nodular lesions, and the boundary was not clear. The diagnosis of all cases was ultimately based on pathological examination. Hematoxylin-eosin (HE) staining images showed active epithelial cell growth with atypia, mitotic figures could be seen, and abundant lymphocyte and plasma cell infiltration could be seen in the tumor stroma. All 22 patients received surgical treatment; 9 patients did not undergo cervical lymph node dissection. Twenty patients received adjuvant radiotherapy after surgery, and 10 of them received adjuvant chemotherapy at the same time. One patient only received chemotherapy after surgery, and one patient did not receive any other adjuvant therapy after surgery. All patients received follow-up visits. One patient died of liver metastasis 16 months after the operation, and the remaining patients survived without tumors for periods of 13 months to 8 years until the present. Conclusion Parotid lymphoepithelial carcinoma is a rare malignant tumor clinically. Pathology is still the gold standard for the diagnosis of lymphoepithelial carcinoma of the parotid gland. Radical resection of the tumor is the first choice of treatment. Selective neck lymph node dissection and postoperative adjuvant radiotherapy and chemotherapy can obtain better therapeutic effects according to clinical examination, imaging examination and neck conditions.

Due to the development of information technology and the need for content analysis of digital humanities research, the use of optical character recognition technology (OCR) to convert contents into verbatim texts can facilitate full-text search and content exploration. In order to understand the feasibility of using the OCR software to convert the full text of the ancient books, this study used the ancient texts to conduct a measured analysis to explore the effectiveness of OCR identification and the reasons for the impact of text recognition. The study selected 40 different layouts and glyphs of Ming Dynasty ancient books for analysis. The results show that the ancient book layout and image quality would affect the OCR recognition rate. When the layout is too crowded and the image quality is blurred, it is not conducive to OCR recognition. This study summarized six common types of identification error glyphs, which can provide the collection agencies to carry out the plan of the full text conversion of similar ancient books.

Objective To investigate the incidence and potential risk factors of postoperative hypoxemia in a post-anesthesia care unit (PACU). Methods The retrospective cohort assessed 14 604 postoperative patients who were admitted to PACU in a period of one year. Clinical data were collected, the incidence and potential risk factors for postoperative hypoxemia were statistically analyzed. Results The incidence of hypoxemia was 2.79%. Multiple regression analysis indicated that the risk factors were age (≥65-year old), body mass index (BMI≥25), American Society of Anesthesiologists (ASA ≥Ⅱ), limb surgery, and thoracic surgery. Conclusions Hypoxemia is common in postoperative patients in PACU. Age, BMI, ASA classification, and surgical site are associated with postoperative hypoxemia. Doctors should pay attention and give timely treatment to high-risk patients to prevent hypoxemia in PACU.

A fumigation with dosage of 20 g/m3 sulfuryl fluoride was carried out in a warehouse stored 8 000 t of wheat and equipped with recirculation system, in which the temperature was 27～30 ℃ for head space,26～28 ℃ for top layer of the bulk, 16～19 ℃ for middle and bottom layers of central bulk and 26～29 ℃for surrounded bulk grain in the warehouse. The fumigant was applied in five points on the bulk surface and through the ventilation ducts under bottom of the bulk by a pipe connection while the recirculation running.The concentrations of sulfuryl fluoride in bulk surface, 1.5 m and 4.5 m depth under grain surface were all monitored during the process. The killing effect on Cryptolestes ferrugineus (Stephens) was also examined for the adults, pupae, larvae, and eggs of lab cultured strain and adults of field strain. The peak concentration was monitored on surface of the bulk and in the depth of 4.5 m of that, the concentration was lower in the depth of 1.5 m of the bulk, just after fumigant applied and while recirculation running. During exposure of 3 to 12 h, the sulfuryl fluoride concentration was above 20 g/m3 and distributed uniformly.The concentration of sulfuryl fluoride in the grain pile was generally higher, and the concentration decreased obviously with the time after 12 h. Sulfuryl fluoride concentration descended more quickly after 24 h when recirculation stopped and descended rate of sulfuryl fluoride concentration in higher temperature part of grain located in surround bulk was bigger than that in lower temperature part located in central bulk. When the concentration of sulfuryl fluoride in the grain pile decreased to 2 g/m3 (about 13 d), the decay rate decreased, and the time from 2 g/m3 to 0 g/m3 was about 10 days. During the fumigation that sulfuryl fluoride concentration changed in range of 27～24 g/m3, adults and larvae can be killed completely in 3 h exposure. Pupae can be given 100% mortality in 6 h when exposure under 27～22 g/m3 of the fumigation. The full mortality for eggs needed 24 h in 27～19 g/m3 of sulfuryl fluoride concentration. The tolerance of four life stages of C. ferrugineus to sulfuryl fluoride was eggs＞pupae＞adults≈larvae. In the grain bulk where temperature was 26～29 ℃ for top layer and surround bulk and 16～19 ℃ for middle and bottom grain of central bulk, all life stages of C.ferrugineus can be fumigated successfully by sulfuryl fluoride that concentration varied from about 24 g/m3 to 0 g/m3 during 23 d of the exposure time.

Objective To study whether blood transfusion with improved blood preservation solution can improve wound healing in diabetic mice, and to investigate its possible mechanism. Methods Kunming mice were used to establish diabetic mice model. They were randomly divided into blood donation group and the experimental group. The blood was placed in sterile centrifugal tube containing anticoagulant and then preserved for 7, 14, 21 and 28 days with improved and standard blood preservation solutions, respectively. The oxygen carrying capacity and deformability of erythrocytes in each preservation period were tested, then the improved and standard autologous blood were retransfused with 7 days of preservation. The experimental group was randomly divided into the standard group and the improved group with 6 mice in each group.The wound healing of two groups of mice was observed on 0, 1, 4, 7 and 14 days, and the mice were killed on 14 days. The degree of fibrosis was analyzed by means of HE, Masson staining and immunohistochemistry methods. Phosphorylation of PI3K,AKT,MEK and ERK were analyzed by Western blot analysis. Results Compared with the standard group, the improvement group exhibited decreased fHb content, and increased P50 oxygen pressure, blood pH, 2,3-Diphosphoglycerate(2, 3-DPG) and red blood cell deformability(RCD)(P＜0.05).HE staining results revealed that compared with the standard group, the area of granular tissue on the skin and the vascular area were increased on the 14th day after operation in the improvement group. Masson staining revealed that, the expression of collagen fibers and the degree of fibrosis in the mice skin of the improvement group were elevated at 14 days after the operation, when compared with the standard group. Immunohistochemistry results indicated that in comparison to standard group, levels of HIF-1α, VEGF, and EGF in skin tissues of the improvement group increased (P＜0.05). The protein levels of PI3K, AKT, MEK,ERK and its phosphorylation increased significantly in the improvement group(P＜0.05). Conclusions Red blood cell treated with improved blood preservation solution may promote wound healing in diabetic mice by activating HIF-1α pathway.

Objective To explore the molecular mechanism of anti-glioma by clofoctol targeting on RNA-binding proteins. Methods Biotin pull-down experiments were used to detect the binding of RNA-binding protein UNR (upstream of N-ras) to the target gene Krüppel-like factor 13(KLF13) mRNA fragments, and the effect of clofoctol on the binding to UNR to KLF13 mRNA fragments; The results of clofoctol DARTS sample's mass spectrometry combined with transcriptome chip analysis were used to screen other RNA-binding protein targets and to predict other downstream target genes. Results Clofoctol enhanced the interaction between UNR and the fragment of KLF13 mRNA; Clofoctol also acted on other RNA-binding proteins, and functioned in mTOR signaling pathway and TNF-related apoptosis-inducing ligand (TRAIL)-activated apoptotic signaling pathway. Conclusions Clofoctol can resist gliomas by acting on RNA-binding proteins.

Soy whey (SW) is generated as a process waste during soy isolate protein preparation. SW has low content of soluble sugar and high content of oligosaccharide, which is suitable for the growth of lactic acid bacteria (LAB). In this study, a strain of Lactobacillus casei GJ00412 (by 16S rRNA genesequencing and identification) was isolated from soy whey. In order to reduce the cost of culture medium, improve its biomass, and facilitate the next utilization, Response surface methodology (RSM) was employed to optimize the composition of seed medium of Lactobacillus casei By Plackett-Burman design, sucrose, yeast power, MgSO4, Tween80 and pH value were found to have significant effects on biomass, and were further analyzed by Box-behnken design, an application of response surface. The optimized medium was as follows (w/v): 2.5% sucrose, 1.8% soy peptone, 0.4% yeast powder, 0.08% MgSO4, 0.04% MnSO4, 0.08% Tween80, pH 7.17. The results showed that the cost of the optimized medium was decreased and the biomass of Lactobacillus casei has no significant difference when compared with MRS medium.

Taking the gear transmission mechanism of a five-gear transmission as the research object,the simulation model of the gear transmission mechanism of the transmission is established by using Romax Designer software,and the load-bearing situation,transmission error and load distribution on the tooth surface of the 4 th gear are simulated and analyzed. According to the theory of tooth lead modification and tooth profile modification,the variable range of three-dimensional modification parameters of the 4 th gear is analyzed by orthogonal experiment principle. Then the genetic algorithm is used to optimize the modification parameters of gear,and the optimal combination of modification parameters is obtained. The validity of this method is verified by vehicle test. By comparing the results before and after optimization,it can be seen that the three-dimensional gear modification technology can greatly reduce the stress of the gear,reduce the fluctuation amplitude of the transmission error of the gear,optimize the stress distribution of the gear tooth surface,and reduce the vibration noise. At the same time,it can be proved that this method can effectively improve the meshing condition of gears and is an effective means to optimize the meshing performance of gears.

In this study, ultra performance liquid chromatography-qiadrupole time-of-flight mass spectrometry was used to analyze the chemical components of the extract of Scutellariae radix, from which 26 flavonoids and flacomols were identified. The main active components including baicalin, scutellarin, wogonoside and wogonin were selected by network pharmacology oriented by drug and disease. The yields of these components were used as the evaluation indexes for extraction process optimization. The influence of process parameters on the indexes was investigated through definitive screening design, so as to establish the design space for the extraction of Scutellariae radix. The recommended operating space was as follows: the time and liquid-solid ratio of the first extraction was 35-45 min and 16-18 mg/L, respectively; for the second extraction, the ethanol volume fraction was 35%-45%, the temperature was 78-82 °C, the extraction time was 45 min and the liquid-solid ratio was 14-16 mg/L. The extraction process was proved to be stable and reliable. This study has improved the correlation between process research and drug clinical practice, which provided ideas for actual quality improvement and process development. [ABSTRACT FROM AUTHOR]

The optimization and kinetic analysis of ultrasound-assisted extraction of polysaccharides from Panax notoginseng residue was studied in this paper. On the basis of single factor experiment, the process was optimized by Box-Behnken Response Surface Methodology. The optimal extraction process was as follows: ultrasound temperature 60 °C, ultrasound power 136.8 W, ultrasound time 53 min, liquid-solid ratio 43 mL/g. Under the optimal conditions, the yield of polysaccharide was 6.79%, while the predicted yield was 6.88% and the relative standard deviation (RSD) was 1.31%. In comparison with the same conditions of hot water extraction, the yield was increased by more than 55% by the optimization. The kinetic model of ultrasound-assisted extraction of polysaccharides from Panax notoginseng residue was built. The rate constant and relative extraction residual rate of polysaccharides was calculated by measuring the concentration of polysaccharides in the extraction solution at different ultrasound powers and different ultrasound times. According to those two parameters, the half-life regression equation and the effective diffusion coefficient regression equation were fitted. The experimental results showed that the rate constant increased with the increasing of ultrasonic power. The enhancement of ultrasonic power was beneficial to the faster dissolution of polysaccharides from Panax notoginseng residue. [ABSTRACT FROM AUTHOR]

Hundreds of millions of bacteria adhere to human intestinal epithelium,which are the participants of human health and physiological functions,and also an important target covered by the concept of comprehensive health.So far,the supplementation of prebiotics remainsare still one of the effective means to regulate intestinal microbiota and promote the growth of beneficial microbes.The by-productsof grain and oil processing are abundant in variety and yield,and many of them can be utilized by human intestinal microbiota,which are one of the sources of high-quality prebiotics,and also the important material basis and raw material source in the development of “microbial-oriented food”.

The dynamic characteristics analysis of damage structure is an important basis for non-destructive testing. However, there are few literatures concerning the study of dynamic characteristics of rotating cracked beams. Taking rotating cracked beams with variable cross-section as the research object, the transverse vibration characteristics of rotating cracked beams are investigated, and a new method to solve the transverse vibration characteristics of rotating cracked beams is proposed. The torsion spring is used to simulate the crack effect, and the flexibility model of cracked beams model is established. The Frobenius method is used to solve the vibration equation, and obtain the series analytic solution of the equation. The effect of crack position and depth on vibration frequency is studied, and the variation of first two-order natural frequency of beam is analyzed under different damage degrees and speeds. The results show that the proposed method is effective, the change of rotating speed and damage degree do not affect the natural frequency of the beam independently, and there is a coupling mechanism between the two items. The conclusions are suitable for beams with variable cross-section.

Rotating beam structure is widely used in the aerospace engineering field. Its health status is directly related to the flight safety of the aircraft. Damage detection of the rotating beam structure can detect the damage in time so as to avoid heavy losses due to structural damage. Taking variable-section rotating cracked beam as an example, the damage identification is based on auxiliary mass method combined with fractal dimension theory. Firstly, the mass-crack beam model is established, and the natural frequency curve is calculated with the mass position changing. Then the fractal dimension curve of natural frequency curve is obtained by using fractal dimension method, and the location and depth of the crack can be detected. Finally, the effects of crack position, crack depth, auxiliary mass and rotating speed are investigated in detail. The simulation results demonstrate the efficiency and accuracy of the proposed method.

The Ni3Al intermetallic compound is considered an excellent wear-resistant material. The addition of Cr3C2 particles can further improve the wear resistance of Ni3Al-based alloys. In order to elucidate the wear mechanism of Cr3C2/Ni3Al composites improved by the Cr3C2 strengthening phase, Ni3Al-alloy and Cr3C2/Ni3Al composites were prepared by the hot isostatic pressing process in this study. The mechanical properties and wear resistance of each phase in the Ni3Al-alloy and Cr3C2/Ni3Al composites were investigated using a nano-indentation instrument and a pin-on-disk friction and wear tester, respectively. The worn surface morphologies and the hardness of the subsurface layer under the worn surfaces of the Ni3Al-alloy and Cr3C2/Ni3Al composites were determined by a scanning electron microscopy (SEM) and a nano-indentation instrument. The results indicate that the hardness of the matrix phase in the Cr3C2/Ni3Al composites is significantly improved by the addition of Cr3C2 particles. The nano-hardness and the elastic modulus of each phase in the Cr3C2/Ni3Al composites gradually increase from matrix phase through diffusion phase to hard core phase. The mechanical properties between the matrix, diffusion, and hard core phases in the Cr3C2/Ni3Al composites present a gradient transition. This kind of structure distribution is good for enhancing the wear resistance of Cr3C2/Ni3Al composite materials. As for friction and wear conditions in this study, abrasive wear was the dominant wear mechanism, which occurred on the surfaces of the Ni3Al-alloy and Cr3C2/Ni3Al composites. The Cr3C2/Ni3Al composites showed a good wear resistant property. The carbide-strengthening phase can block up the cutting action of the wear debris, reduce the interaction between the wear materials, and decrease the thickness of the subsurface layer and the size of the wear debris, resulting in improved wear resistance of Cr3C2/Ni3Al composites.

A kind of alumina ceramic was prepared by atmospheric pressure sintering under air atmosphere using MgO-CuO-TiO2-Eu2O3 as sintering additive. The effect of Eu2O3 doping amount and sintering temperature on the phase composition, microstructure as well as dielectric properties of Al2O3-based microwave ceramic samples was studied by XRD, SEM and EDS. The results show that the Al2Eu2O9 secondary crystalline phase is present in all Al2O3 ceramics with Eu2O3 addition, and the Al2Eu2O9 phase is enhanced with the increase of Eu2O3 content. With higher Eu2O3 content, the density of Al2O3 ceramics increases first and then decreases. As sintering temperature increases, the dielectric constant and quality factor Q·f of Al2O3 ceramics increase first and then decrease. When the sintering temperature rises up to 1450℃ and the content of Eu2O3 is 0.25%(mass fraction), the relative density of the sintered body reaches to the maximum of 98.21%, and the dielectric properties of the Al2O3 ceramics are superior with the dielectric constant 10.05 and the quality factor Q·f 37984GHz.

Acidolysis hydrocarbon is an important index with relatively high accuracy in the geochemical exploration for oil and gas and has been widely used. Detailed study of acid-hydrolyzed gases can help further explain its mechanism and help develop new analytical indicators. The content of acidolysis hydrocarbons and other gas released by hydrochloric acid in the samples from geochemical exploration for oil and gas by Gas Chromatography have been determined, and are presented in this paper. There is a positive correlation between the contents of methane and non hydrocarbon gas. The elements and mineral composition of the sample were measured by X-ray Fluorescence Spectrometry (XRF) and X-ray Diffraction (XRD), respectively, whereas the gas-liquid inclusion composition was analyzed by Laser Raman Spectroscopy after sample smashing. The conclusions are as follows:(1) The content of methane and hydrogen in the acidolysis hydrocarbons is independent of the elemental composition of the sample and is positively related to the carbonate minerals. The contents of methane and hydrogen can reflect the situation of deep oil and gas. (2) Two kinds of gas do not exist in the form of physical adsorption but in the smaller space. It is proved that the analysis method of acid hydrolysis hydrocarbon has high stability. (3) There is direct evidence that two kinds of gases exist in the inclusions at the same time, which infers that two types of gases have the same source. Due to the correlation between methane and hydrogen, the content of hydrogen from acidolysis hydrocarbons will be a new analysis index in the geochemical exploration for oil and gas.

Helium is an indispensable and rare strategic element. A method of sampling the top helium gas in the helium gas resource investigation was used, and is described in this paper. Thermal conductivity detector (TCD) of the Gas Chromatograph was used to detect helium gas. The helium gas standard was used to study the helium gas storage container, the storage medium, the storage temperature, and the storage time. Results show that the glass jar, the saturated salt water, the low temperature, and the inverted storage are the best methods to save the head space gas. At room temperature, the helium head space gas storage period is about 2 months, whereas under refrigerated conditions (4℃), the storage time reaches 3 months. Therefore, the author suggests that the helium head space gas collection method is as follows. The fresh soil samples are collected in a 500 mL glass bottle, and then are injected into the saturated salt water. A 50 mL space remains in the bottle, and then the bottle is sealed by silica gel and kept inverted. For the helium top gas stored under normal temperature and refrigerated conditions, the test should be completed within the corresponding storage period to ensure the accuracy of the test data.

Entrained flow bed gasification is an important way to realize clean and efficient utilization of coal. However, Shell entrained flow bed gasifier uses Shangwan coal with low ash melting temperature, resulting in low gasification efficiency of gasifier. In order to improve the gasification efficiency of Shell gasifier, the characteristics of Shangwan coal were adjusted by adding promoter kaolin and mixing Wuhai coal. The effects of kaolin addition ratio and mixing Wuhai coal on viscose-temperature characteristics and melting characteristics of Shangwan coal ash were studied. The viscosity-temperature characteristics of coal ash samples were analyzed by the high temperature rotating viscometer, the melting characteristics of coal ash samples were analyzed by the ash melting point tester, and the particle size distribution of fly ash of Shangwu mixed coal was tested by the laser particle size analyzer. The results show that when the mass ratio of kaolin to Shangwan coal ash increases from 0.0:10.0 to 7.5:10.0, the acid-base ratio increased from 0.99 to 2.40, the flow temperature of coal ash in Shangwan increases from 1124 °C to 1375 °C, and the critical viscosity temperature increased from 1140 °C to 1420 °C. When the mass ratio of kaolin to Shangwan coal ash is 3.0:10.0, the target gasification operating temperature is 1500 °C, which accords with the operating temperature of Shell gasifier with high carbon conversion rate. When the mass ratio of Shangwan coal and Wuhai coal is 8:1, the ash acid-base ratio of Shangwu mixed coal is 1.94, the target gasification operating temperature is 1550 °C, the gasification operating window is 150 °C, and the particle size of gasification fly ash of Shangwu mixed coal is concentrated in the vicinity of 1 µm, 45 µm and 350 µm, which helps to achieve high carbon conversion and long cycle operation of gasifier. [ABSTRACT FROM AUTHOR]

Aiming at the problem of the recovery sequencing and scheduling of the carrier aircraft fleet landing, the carrier deck environment and the return recovery approach mode of the carrier aircraft are analyzed firstly, and the evaluation index model of the recovery sequencing and scheduling based on the weighted waiting time is established. According to the various constraints in the recovery sequencing and scheduling problem of the carrier aircraft, the model of the recovery sequencing and scheduling problem of the carrier aircraft considering the air refueling condition is established. Then, according to the established recycling sequencing and scheduling model and the idea of huper-heuristic algorithm, a genetic programming with mandatory landing rules (MGP) algorithm is designed to solve the ship necovery scheduling problem. Further, the effectiveness of the proposed recovery sequencing and scheduling model and MGP algorithm is verified by simulation examples, and the advantages of MGP algorithm are verified by comparison with genetic algorithm, pure heuristic algorithm and common genetic programming algorithm. Finally, based on the simulation results of an example, the influence of escape and wave off on the landing fleet recovery scheme is analyzed. [ABSTRACT FROM AUTHOR]

The inherent Doppler blind zone (DBZ) of airborne early warning radar can bring out target track termination with the worse effect in multi-target and clutter environment. Based on two-dimension assignment algorithm with DBZ information, a new plot-to-track association method based on multi-dimension assignment algorithm is proposed. Firstly, the evolution information of target track is used enough, and the radar's continuous multi-frame measurement data are used to perform sliding window processing. Secondly, the cost function of multi-dimension assignment is constructed. Finally, the solution of function on the condition of minimal cost is used as the targets plot-to-track assignment solution. The simulation results show that this method has further descent of track termination rate compared with two-dimension assignment algorithm in multi-target and clutter environment with DBZ. [ABSTRACT FROM AUTHOR]

A The Ni3Al-alloy and its composites with different Cr3C2 contents were fabricated by a hot isostatic pressing (HIP) technique. The influences of Cr3C2 content on the microstructure, hardness and wear properties of the Cr3C2/Ni3 Al composites were investigated by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and wear tribometry. The results show that inter-diffusion occurs between the original Cr3C2 and the Ni3AI particles during the HIP process and the Cr3C2 particles partially transform into M7C3 (M=Cr, Fe, Ni) structures. Under specific friction and wear conditions, the wear resistance of the Cr3C2/Ni3Al composites is significantly improved due to the addition of Cr3C2 particles by about 4-10 times, compared to the Ni3Al alloy. In addition, the cutting and scraping effects of counter-part disks by the Cr3C2/Ni3Al composites decreases with increasing Cr3C2 addition, resulting in a reduction in wear rate of counter-part disks.

Statistic distribution of fatigue life data and its controls of cast Ti-47.5Al-2.5V-1.0Cr-0.2Zr (atom fraction/%) alloy were investigated. Fatigue tests were operated by means of load-controlled rotating bending fatigue tests (R=-1) performed at a frequency of 100 Hz at 750 ℃ in air. The fracture mechanism was analyzed by observing the fracture surface morphologies through scanning electron microscope,and the achieved fatigue life data were analyzed by Weibull statistics. The results show that the fatigue life data present a remarkable scatter ranging from 103 to 106 cycles, and distribute mainly in short and long life regime. The reason for this phenomenon is that the fatigue crack initiators are different with different specimens. The crack initiators for short-life specimens are caused by shrinkage porosity, and for long-life ones are caused by bridged porosity interface and soft-oriented lamellar interface. Based on the observation results of fracture surface, two-parameter Weibull distribution model for fatigue life data can be used for the prediction of fatigue life at a certain failure probability. It has also shown that the shrinkage porosity causes the most detrimental effect to fatigue life.

Put forward a kind of skin structure crane optimization design method based on reliability analysis. Considering the randomness of size parameters,material properties and wind load in the skin structure crane design,using Monte-Carlo method of Latin hypercube sampling（ LHS） technique to calculate the reliability of structure system. Through probabilistic sensitivity analysis to determine the optimal design variables,with the total quality of metal structural system of skin structure crane as the goal,to optimize the design by using the finite element analysis software ANSYS,relative to the initial design the quality of skin structure is reduced by 23. 8%,the overall reliability is used effectively,avoids the local reliability surplus,saves manufacturing cost.

Based on the theory of random vibration,the dynamic response on tower crane under random seismic excitation was investigated. The input of earthquake ground motion is indicated by Duxiuli-Chenhouqun power spectral. The random seismic responses of structural ’s force and displacement were researched under five different earthquake model input with uniform earthquake excitations method. The analysis reveals that the force of tower crane increase significantly under vertical seismic excitation than X-direction,Z-direction excitation have the greatest influence in tower body bending moment; the primary reason of tower arm fracture is vertical seismic action,too large seismic wave cause tower body capsized easily.

For the special requirements of anomaly detection in backbone networks,an anomaly detection method was proposed based in the summary data structure: Filter-ary-Sketch.It recorded the network traffic information in Fil-ter-are-Sketch online and detected anomalies based on multi-dimensional entropy at every circle.If an anomaly was detected,the anomaly point located according to data stream recorded in Filter-ary-Sketch.Finally,malicious traffic blocked using the source IPs recorded in Bloom filter.The method was effective in detecting a variety of network at-tacks;especially it could block the malicious traffic.Evaluated by the experiment,the method can detect anomaly in the backbone network with small computing and memory resource and block the IP flows that are responsible for the anomaly.

Annual wild soybean is the ancestor of cultivated soybean. The 100-seed weight gradually increases in the long-term domestication process. Clarifying the genetic basis of this change is of great significance to the evolutionary research and variety improvement of soybean. In order to analyze the genetic basis of 100-seed weight during soybean domestication, a wild soybean chromosome segment substitution line population (SojaCSSLP5) composed of 177 whole-genome resequencing lines were used in this study. 13 QTLs/segments of 100-seed weight were detected by phenotypic evaluation in three different environments. All of 13 wild chromosome segments had the additive effect of reducing 100-seed weight, ranging from -0.49 g to -1.19 g, which was consistent with the smaller 100-seed weight of wild soybeans. These detected domesticated segments from 11 chromosomes explained 76.70% of the phenotypic variation, and the phenotypic contribution rate of a single segment ranged from 2.45% to 15.14%. The contribution rate of segments Gm03_LDB_15 and Gm12_LDB_46 exceeded 10%, which were major influence on the evolution of 100-seed weight of wild soybeans. Combined the transcriptome data and genome data of parental cultivated soybean Nannong 1138-2 and wild soybean N24852, a total of 13 candidate genes were predicted in these segments, and were involved in the pathways of plant seed size, including ubiquitin protein kinase regulatory pathway, G protein signal pathway, mitogen-activated protein kinase pathway, plant hormone pathway, transcription regulator pathway, and IKU (HAIKU) pathway. Compared with previous QTLs mapping results with cultivated soybeans, 4 of the 13 QTLs/segments were newly detected in this study, indicating that 9 wild chromosome segments might be passed to cultivated soybeans during domestication, and the corresponding cultivated segments of these 4 wild segments may be unique evolutionary segments of cultivated soybean. [ABSTRACT FROM AUTHOR]