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Objective To investigate the effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF) innovative preservative on hair follicle activity of rat vibrissae under low temperature conditions. Methods From January 2021 to December 2023, the upper of hair follicle (UHF) from male SD rat vibrissae was used in the experiment. These UHF were equally divided into NS, IGF-1, EGF, and IGF-1+EGF preservation solutions groups. At 3, 6 and 12 hours, the cultured UHF were analyzed by hematoxylin and eosin (HE) staining and alkaline phosphatase (ALP) activity. The UHF co-cultured with NS and IGF-1+EGF for 3 hours were further transplanted into the dorsum of nude mice. After 24 hours, the transplanted UHF was taken to observe by HE staining and ALP activity. Results In vitro preservation, HE staining results indicated that the cultured UHF at 3 hours, the follicle structure and cell arrangement were normal without obvious changes. At 12 hours, only the IGF-1+EGF group had intact follicle structure and clear organization. Moreover, ALP values showed that the IGF-1+EGF group at 3, 6 and 12 hours were significantly higher than the NS group (P＜0.001). Overall, ALP activity gradually decreased over time. In vivo transplantation, HE staining results showed that the IGF-1+EGF group were structurally distinct with orderly cell arrangements, compared to the NS group. ALP values were significantly higher than NS group (P＜0.05). Conclusion The innovative preservation solutions containing IGF-1 and EGF under low temperatures, can effectively enhance the activity of UHF, and promote the success of hair follicle transplantation. [ABSTRACT FROM AUTHOR]

The efficacy of yeast/zinc fermentation products in scalp care were studied from the aspects of safety, oil control and dandruff removal, and hair follicle maintenance. The safety of yeast/zinc fermentation products was tested through human patch testing. In vitro SZ95 cell model was used to study the oil-control efficacy of yeast/zinc fermentation products. The method of qRT-PCR was used to verify the expression of oil-secretion-related genes SREBP-1c, PPARG, and PI3K. Spectrophotometry was used to test the effect of yeast/zinc fermentation products on 5α-reductase. The oil-control effect of yeast/zinc fermentation products on scalp and the maintenance effect on hair follicles were studied through human efficacy testing and in vitro hair follicle model, respectively. The results showed that the yeast/zinc fermentation products at a content of 5% was safe and harmless to human health; the yeast/zinc fermentation products with a content of 0.8%-1.5% could significantly inhibit the activity of 5α-reductase. The yeast/ zinc fermentation products at a content of 0.5%-2.0% could significantly inhibit the secretion of oil in SZ95 cells in vitro, and significantly downregulate the expression of oil-secretion-related genes SREBP-1c and PPARG. The yeast/ zinc fermentation products at content of 1.0% and 2.0% could significantly inhibit the expression of PI3K. The yeast/zinc fermentation products at a content of 5% could significantly reduce scalp oil secretion, improve hair follicle cycle, and promote hair shaft growth. Therefore, the yeast/zinc fermentation products had certain scalp care effects and had positive effects in controlling oil, improving hair follicle health, and promoting hair shaft growth. [ABSTRACT FROM AUTHOR]

The purpose of this study was to study the effects of early feeding of diets with different protein levels balanced methionine on the growth performance, feather-related hormones, skin antioxidant capacity, and hair follicle traits in Wanxi white geese. Ninety one-day-old white geese with similar weight, half male and half female, were randomly divided into three groups, with five replicates in each group and six geese in each replicate. The geese were fed with 19.93%, 18.01% and 15.95% crude protein diets, respectively, and the methionine content was 0.5% in each group. The experimental period was 14 days. The results showed that the serum luteinizing hormone (LH) content in the 18.01% CP group was significantly higher than that in the 19.93% CP group (P<0.05), and extremely higher than that in the 15.95% CP group (P<0.01). The total antioxidant capacity (T-AOC) in the chest skin of the 18.01% CP group was significantly higher than that of the 19.93% CP group (P<0.01), and the glutathione peroxidase (GSH-Px) activity in the chest skin of the 18.01% CP and 15.95% CP groups was significantly higher than that of the 19.93% CP group (P<0.05), and the activities of superoxide dismutase (SOD) was extremely higher than that of the 19.93% CP group (P<0.01). The diameter of abdominal primary follicles in the 18.01% CP group was significantly higher than that in the other two groups (P<0.05), the diameter of the chest secondary follicles in the 15.95% CP group was significantly lower than that in the other two groups (P<0.01), and the diameter of abdominal secondary follicles in the 19.93% CP group was significantly lower than that in the 18.01% CP group (P<0.01). The density of the barb in the back of the 18.01% CP group and the 15.95% CP group was significantly lower than that of the 19.93% CP group (P<0.01). The results show that when the methionine content in the diet met the body requirements of the Wanxi white geese (0.5%), the appropriate reduction of the protein level in the diet (18.01%) can't affect the growth performance of the goslings, and can promote the expression of luteinizing hormone in the serum, improve the antioxidant capacity in the skin, regulate the feather growth performance of the Wanxi white geese, improve the quality of feather, and finally achieve the effect of reducing breeding costs and maintaining down production. [ABSTRACT FROM AUTHOR]

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Hair follicle is a regenerative organ, and its growth process constantly goes through the cycle of growth, decline and rest period. The cyclic growth of hair follicle determines the growth and loss of fur. Hair follicles are not only regulated by genetic, environmental, and other factors, but also closely related to the content and type of nutrients in the diet, especially the protein content and amino acid composition. Protein is an important substance in the composition of fur animal coat. Sulfur-containing amino acids are the main amino acids that restrict the growth of coat. Sulfur elements exist in the coat in the form of disulfide bonds, which play an important role in maintaining the network space structure of coat fibrin molecules and resisting the erosion of various adverse factors from the outside world. In addition, lysine, tryptophan, tyrosine, glutamic acid, and other non-sulfur-containing amino acids are also involved in the formation of fur animal skin sheets, which play an important role in controlling fur animal skin coat color, promoting coat growth and regulating fur maturation. In this paper, the recent research progress on the effects of protein content and amino acid composition in feed on fur quality of fur animals is reviewed, in order to provide a reference for guiding the genetic selection and production of fur animals. [ABSTRACT FROM AUTHOR]

BACKGROUND: Combined radiation and wound injury appeared mainly in patients with tumor radiotherapy and nuclear radiation accidents. The radiation destroys the repair mechanism, resulting in delayed or prolonged wound healing. It still lacks an effective therapeutic strategy currently. OBJECTIVE: To prepare multifunctional wound dressings based on the multiple clinical symptoms of combined radiation and wound injury, which are designed to be antibacteria, promoted healing and analgesics. METHODS: Using levofloxacin, fibroin and lidocaine hydrochloride as raw materials, 3D bioprinting technology was applied to prepare the multifunctional wound dressing. (1) The multifunctional dressing was placed on a fixed culture plate coated with Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and incubated at 37 °C overnight to detect the diameter of the antibacterial zone. (2) 40 Kunming mice were randomly divided into trauma group, radiation and trauma model group, treatment group and positive drug group, with 10 mice in each group. Mice in the radiation and trauma model group, treatment group and positive drug group were irradiated by 60Co gamma rays. After 1 hour of radiation, a full-layer skin defect wound with a diameter of 1 cm was made on the back of each mouse in the four groups. Normal saline was applied to the wounds of the trauma group and the radiation and trauma model group. Trethanolamine cream was applied to the wounds of the positive drug group. Multifunctional dressing was applied to the wounds of the treatment group. The dressing was changed every 2 days, and the treatment was continued for 14 days. Wound healing rate and serum interleukin-6 level were measured at 3, 7 and 14 days after wound modeling. 14 days after the wound modeling, the skin tissue of the wound was obtained and received hematoxylin-eosin staining, Masson staining and cytokeratin-14 immunohistochemical staining. RESULTS AND CONCLUSION: (1) 3D-printed multifunctional wound dressing had good antibacterial activity. The antibacterial zone diameters against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were (4.15±0.09), (4.18±0.23) and (4.35±0.13) cm, respectively. (2) With the extension of modeling time, the wound healed gradually. The wound healing rate of the treatment group and the positive drug group was higher than that of the radiation and trauma model group at 3, 7 and 14 days after modeling (P < 0.01, P < 0.001). The wound healing rate of the treatment group was higher than that of the positive drug group. With the extension of modeling time, the serum interleukin level of mice increased first and then decreased. The serum interleukin level in the treatment group at 3, 7 and 14 days after modeling was lower than that in the radiation and trauma model group. Hematoxylin-eosin staining and Masson staining exhibited that inflammatory cells infiltrated the granuloma tissue in the trauma group, and the dermal collagen fibers were densely arranged. The normal structure of epidermis and dermis was destroyed and inflammatory cells were infiltrated in the radiation and trauma model group. In the treatment group, normal skin mucosal tissue was observed, the epidermis was arranged closely, and the sweat glands, hair follicles and dermal collagen fibers were arranged regularly. In the positive drug group, the arrangement of epidermal layer was tight, and the arrangement of sweat glands, hair follicles and dermal collagen fibers was regular. Cytokeratin-14 immunohistochemical staining displayed that the epidermal tissue thickness in the treatment group was lower than that in the other three groups (P < 0.01, P < 0.001). (3) The results confirm that the 3D-printed multifunctional dressing has multiple functions of local anesthesia, anti-infection and promoting healing. [ABSTRACT FROM AUTHOR]

BACKGROUND: Autophagy has become a rapidly developing research hotspot in the biomedical fields. Many researchers are actively exploring the molecular regulatory mechanism of autophagy in a variety of diseases. However, the role of autophagy in hair growth is still unknown. OBJECTIVE: To review the current research progress and application value of autophagy in hair growth and regeneration, to understand the role of autophagy in hair growth, to explore the pathogenesis of autophagy in pathological hair loss, and to provide new ideas for the study of drugs for hair loss. METHODS: Using “hair follicle growth, hair growth, hair regeneration, autophagy associated proteins, autophagy activity, autophagy associated genes, autophagy” as Chinese search terms and “hair growth, hair follicle, hair regeneration, autophagy” as English search terms, PubMed and CNKI databases were searched. The research progress on autophagy, hair growth and the role of autophagy in hair growth in and outside China in recent years was reviewed and summarized. Articles incompatible with the subject content of the paper were excluded. Finally, 78 articles were included for the result analysis. RESULTS AND CONCLUSION: (1) Autophagy is a normal metabolic process in eukaryotes with complex molecular mechanisms and functional properties, which is beneficial to cell survival and cell death. (2) Alopecia-related diseases are associated with changes in autophagy activity, which can regulate hair growth cycle. Knockout or overexpression of autophagy-related genes can change the state of hair growth. Multiple autophagy related signaling pathways have been found to be related to hair follicle growth. Activators or inhibitors of autophagy can be used to treat or prevent hair loss. [ABSTRACT FROM AUTHOR]

Copyright of China Surfactant Detergent & Cosmetics (2097-2806) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Image thinking in traditional Chinese medicine ( TCM) is a way of thinking to establish connections with corresponding “images” and to explore the links between the essential laws of TCM and the material attributes, laws, and properties known in nature. Based on traditional image thinking, we compare hair to trees and combine TCM with the modern understanding of androgenetic alopecia to describe its pathogenesis. It is believed that plants and trees wither in dry conditions. This is similar to a lack of kidney essence; when blood can’t be transformed into essence,resulting in damage to emptiness of hair follicles, hair shrivels and falls off. Similarly, plants and trees can die due to excessive water over a long period of time, which is analogous to steaming on the parietal bone due to dampness-heat; the hair root is soaked, becomes soft, and dies. In other cases, because of soil hardening and poor permeability, the root cannot grow, which is analogous to blood stasis in the hair follicle; the flow of qi in the meridians is not smooth and the hair roots are not moistened, resulting in hair loss. The treatment of androgenic alopecia is therefore analogous to the treatment of natural plants and trees. Plants and trees need to be fertilized and irrigated; in analogy, the kidney should be nourished to secure the root so as to replenish yin and grow hair. Plants and trees need water drainage; similarly, turbidity and the fat should be transformed and reduced to strengthen the roots. Plants and trees need loose soil; in analogy, in the scalp, blood stasis should be removed to improve the blood supply of hair follicles. Furthermore, new hairs are like seedlings; therefore, we should pay attention to disease aftercare. This article discusses the treatment of androgenic alopecia based on image thinking in order to provide a new perspective for the clinical treatment of androgenic alopecia. [ABSTRACT FROM AUTHOR]

Objective: The purpose of this study was to explore the therapeutic effect and mechanism of cycloastragenol（CAG） on vitiligo. Methods: The normal human skin melanocytes PIG1 were divided into the following groups: control group, H2O2 group, 10CAG group, 50CAG group, 100CAG group. PIG1 cells were cultured with different concentrations（10, 50, 100 μM） of cycloastragenol and250 μM of H2O2, respectively. Cell proliferation was detected by CCK-8 method, apoptosis was detected by Annexin V-FITC/PI method,and the contents of melanin, SOD, CAT and MDA in cells were determined. C57BL/6 mice were randomly divided into control group,Model group and CAG group. The vitiligo mouse model was established by applying 50 mg of 40% monobenzone cream daily, and then the mice were treated with 50 mg/kg cycloastragenol. Hair follicles and melanin content in skin tissue were detected by HE staining and Masson-Fontana staining. The protein expressions of MITF, TYR, TRP-1, TRP-2, Bax, Bcl-2, p-p38/p38 were detected by Western blotting. Results: Compared with the H2O2 group, the 50CAG group and 100CAG groups had increased cell viability and melanin content,decreased apoptosis rate, decreased Bax protein expression, and increased Bcl-2 protein expression, increased SOD and CAT levels, and decreased MDA levels（P＜0.05). The content of hair follicles and melanin in 10CAG group, 50CAG group and 100CAG group were higher than those in H2O2 group. Compared with the H2O2 group, the protein expression levels of MITF, TYR, TRP-1, TRP-2 and p-p38/p38 in the 50CAG group and 100CAG group were all increased（P＜0.05). Compared with the model group, the protein expression levels of MITF, TYR, TRP-1, TRP-2 and p-p38/p38 in the CAG group were all increased（P＜0.05). Conclusion: Cycloastragenol can promote melanin synthesis at both cellular and animal levels, and has a good therapeutic effect on vitiligo. The mechanism may be related to the activation of p38 signaling pathway. [ABSTRACT FROM AUTHOR]

Clinical data from patients with eyebrow deficiency repaired by autologous hair transplantation in the Department of Plastic and Cosmetic Surgery between January 2015 to February 2021 were retrospectively analyzed. The age, etiology, gender, degree of eyebrow loss and surgical records of patients were collected. The postoperative complications (such as swelling, pain and congestion), loss rate and survival rate of hair, times and proportion of hair transplantation and patient satisfaction were followed-up. Results A total of 167 patients with eyebrow deficiency were included in this study. The amount of transplanted hair follicles was from 50 to 600, with an average of 330. The operative time lasted from 30 to 180 minutes. There were 28 patients (16.8%) underwent the second eyebrow transplantation. The average survival rate of eyebrows in the recipient site ranged from 70% to 90%, with an average of 85%. The wounds in the donor sites healed well. No postoperative complications were found in all patients. The satisfaction rate was 99.4% (166/167). Conclusion The autologous hair transplantation is safe and efficient in the repair of eyebrow deficiency. [ABSTRACT FROM AUTHOR]

[Objective] The experiment aimed to study the effects of bone morphogenetic protein 4 (BMP4) on the genes related to hair follicle development in rabbits.[Method] In this study, rabbit hair papilla cells were isolated by microdissection and two-step enzyme digestion, and the expression of specific markers α-SMA and VIM were detected by immunofluorescence staining. The pcDNA3.1-BMP4 overexpression vector was constructed and transfected into dermopapilla cells, and the effects of BMP4 gene on the mRNA expression of hair follicle developing-related genes (ID1, ID2, SAMD7, FGF1, TGF-β) were analyzed by fluorescence quantitative PCR. The effect of BMP4 gene on the growth of rabbit hair papilla cells was verified by CCK-8 cell proliferation assay. [Result] Immunofluorescence showed that the cultured cells were dermal papilla cells derived from hair follicle dermal skin. BMP4 gene was cloned successfully. The CDS of BMP4 gene was 1230 bp and encoded 409 amino acids. This protein was predicted to be a hydrophilic exsecretory protein with 1 signal peptide sequence and no transmembrane domain. Fluorescence quantitative PCR showed that BMP4 overexpression significantly increased the mRNA expression levels of ID1, ID2 and SAMD7 (P<0.01), but significantly inhibited that of FGF1 (P<0.05). In CCK-8 cell proliferation assay, BMP4 overexpression significantly inhibited the proliferation of dermal papilla cells (P<0.01) .[Conclusion] In conclusion, BMP4 gene may inhibit the proliferation of dermal papilla cells and participate in the development of rabbit hair follicles by affecting the expression of genes related to hair follicle development in dermal papilla cells. [ABSTRACT FROM AUTHOR]

BACKGROUND: Dermal extracellular matrix (d-ECM) has been widely used in clinical wound repair, but whether dermal extracellular matrix hydrogel (d-ECMH) can improve acute skin wound healing is still a lack of relevant research. OBJECTIVE: To explore the healing effect and mechanism of d-ECMH on acute skin wounds in rats. METHODS: Twenty-four SD rats were divided into model group, d-ECM group and d-ECMH group by random number table method, with 8 rats in each group. Firstly, all rats established a full-thickness skin defect model, and then the model group and d-ECMH group were immediately injected with equal volumes of PBS buffer and d-ECMH on the wound respectively after the operation, and the wound surface of the d-ECM group was covered with d-ECM to observe and evaluate wound healing. All rats were sacrificed on day 14, and the skin tissues of the wounds were subjected to hematoxylin-eosin staining, Masson staining, histoimmunochemistry and fluorescence staining. The experiment was approved by Animal Ethics Committee of General Hospital of Western Theater Command. RESULTS AND CONCLUSION: (1) The wound of rats in each group shrank with the prolongation of treatment time. Compared with the model group and the d-ECM group, the d-ECMH group had higher wound healing rate and wound surface skinization rate (P < 0.05), and reduced wound contracture rate (P < 0.05). (2) Hematoxylin-eosin staining showed that compared with the model group and the d-ECM group, the d-ECMH group had better skin wound repair and a higher degree of re-epithelialization of the wound, forming new skin appendages (such as hair follicles), and the matrix of dermis was more uniform. (3) Masson staining results showed that compared with the model and the d-ECM groups, the collagen fibers in the skin wound tissue were significantly increased and thicker in the d-ECMH group. (4) Immunohistochemical staining showed that the number of new capillaries in the skin wound tissue of the d-ECMH group was significantly more than that of the model group and d-ECM group (P < 0.05). The contents of tumor necrosis factor-α and interleukin-6 in wound tissues of the d-ECMH group were significantly lower than those in the model group and d-ECM group (P < 0.05). (5) Immunofluorescence staining showed that the number of M2 macrophages in the new wounds of the d-ECMH group was significantly higher than that of the model group and d-ECM group (P < 0.05), while the number of M1 macrophages was significantly lower than that of the model group and d-ECM group (P < 0.05). (6) The results show that d-ECMH can accelerate skin wound healing, promote wound re-epithelialization, and reduce wound contracture, which may be related to promote capillary angiogenesis and collagen fiber production, reduce inflammation and regulate the polarization of macrophages to M2 in the wound. [ABSTRACT FROM AUTHOR]

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the technical characteristics, operation skills, patient satisfaction and clinical value of interval cut nonshaven follicular unit extraction. Methods Ten patients underwent hair transplantation in our department from September to November 2020 were selected as the research subjects. The hair follicles were extracted by interval cut nonshaven method, and the syringe needle was used to assist drilling and planting. The hair follicle injury rate and operative duration were recorded and the patient satisfaction was analyzed. Results The hair follicle injury rate of interval cut nonshaven follicular unit extraction was similar to that of traditional follicular unit extraction, but took a long time. Patients were satisfied with the efficacy. Conclusion Compared with the traditional follicular unit extraction, interval cut nonshaven follicular unit extraction took a long time. The hair follicle injury rate is similar to traditional follicular unit extraction and patient satisfaction is high. The interval cut nonshaven folllicular unit extraction is suitable for hair transplantation of small area. This method is easier to be accepted. [ABSTRACT FROM AUTHOR]

BACKGROUND: In the skin and hair pigmentation mechanism, diacylglycerol kinase ζ (DGKζ) has not been reported to participate in pigmentation. OBJECTIVE: To investigate the expression and localization of DGKζ and protein kinase C βII (PKCβII) in the mouse back skin with different coat colors and their correlation with the formation of coat color. METHODS: The back skin of 2-month-old mice with different coat colors (white, gray, and black, 6 mice for each color) was taken to detect DGKζ and PKCβII mRNAs by real-time PCR, DGKζ, PKCβII and p-PKCβII proteins by western blot, and DGKζ and p-PKCβII expression by immunohistochemistry. The study protocol was approved by the Animal Ethics Committee of the Animal Experimental Center of Shanxi Medical University. RESULTS AND CONCLUSION: DGKζ and PKCβII mRNAs were both expressed in the back skin of mice with three kinds of coat colors, with its relative expression amount being: gray group > white group; black group > white group (P < 0.05). Both DGKζ and PKCβII proteins were expressed in the back skin of mice with different hair coat colors. The ratio of p-PKCβII to PKCβII showed that the change of PKCβII activity had the same tendency with DGKζ expression: gray group > white group; black group > white group (P < 0.05). Immunohistochemistry staining showed that DGKζ and p-PKCβII were positively expressed in the outer root sheath of hair follicle, hair matrix cells and melanocytes in the hair matrix in the back skin of gray and black mice, while were negative in the hair follicles of white mice. According to the expression and localization of DGKζ, PKCβII in white, gray and black mouse back skin, we can speculate that DGKζ and PKCβII may participate in the formation of the coat color. [ABSTRACT FROM AUTHOR]

[ Objective]The present paper aimed to study the structure and developmental changes of hair follicles during the fetal period of sheep, and provide basis for regulating the periodic growth of hair follicles and improving wool yield and quality. [ Method ]33 developmental fetal sheep skin tissues of Chinese Merino sheep ( Xinjiang Junken type) were collected from 4S, SS, 6S, 7S, 8S, 9S, lOS, 1 lS, 12S, 13S and 14S days fetus. The paraffin sections of skin and hair follicles were prepared with 3 individuals each period. After HE staining, the skin and hair follicle morphology of fetal sheep in 11 developmental stages were observed under a microscope. The primary follicle density, secondary follicle density and secondary to primary follicle ratio ( S/P) were counted and statistical analyzed. [Result] The developmental time of skin epidermis, dermis, and subcutaneous connective tissue was 4S -day fetus. The skin was developed completely in SS-day fetus. Primary hair follicles began to develop into the vesicles in 6S-day fetus. Primary hair follicles were formed in 7S-day fetus. Secondary hair follicles began to develop in 8S-day fetus, and the number of primary hair follicles was increased rapidly. In lOS-day fetus, the primary secondary follicles began to differentiate into secondary follicles. The primary and secondary hair follicles were mature in 13S to 14S-day fetus. The density of primary hair follicles was decreased from 8S to 14S-day fetus. However, the density of secondary hair follicles and S/P value were increased from 8S to 14S-day fetus. [ Conclusion ] The developmental time of primary hair follicles was 6S-day fetus, the developmental time of secondary hair follicle was 8S-day fetus, and redifferentiation time of secondary hair follicles was lOS-day fetus. [ABSTRACT FROM AUTHOR]

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Wnt5a is the only signaling molecule that is highly expressed in the dermis and closely related to the occurrence of hair follicle dermal components. OBJECTIVE: To preliminarily discuss initiation mechanism of dermal papilla embryogenesis and regeneration in vitro by observing Wnt5a expression of E12.5-E15.5 fetal rat skin and the upper part of tentacle hair follicle during dermal papilla regeneration induced by transplantation. METHODS: The pregnant Sprague-Dawley rats at E12.5, E13.5, E14.5 and E15.5 days were killed under anesthesia. The embryos were taken out to make paraffin sections. The hairy papilla was removed from the tentacle hair follicles of Sprague-Dawley rats, and the hair follicles were removed from the upper segment of the hair follicles and then injected into the skin of the back of Nu/Nu nude mice. The skins of rat embryos at above gestational age and hair follicles at transplanted back positions of the nude mice were taken. Enzymatic immunohistochemical method was used to observe the Wnt5a expression in rat embryonic skin and the upper segment of hair follicle during dermal papilla regeneration induced by transplantation. Hematoxylin-eosin staining was used to observe the regeneration of the inferior segment of hair follicle after transplantation in the upper segment. The animal experiment was approved by the Animal Ethics Committee of Shantou University Medical College. RESULTS AND CONCLUSION: (1) E12.5 Wnt5a was expressed in epidermal cells; E13.5 Wnt5a was expressed in epidermal cells and enhanced; E14.5 Wnt5a was transferred from epidermis to dermis and expressed in dermal cytoplasm; E15.5 Wnt5a was expressed in dermal connective tissue, obviously enhanced, and disappeared in epidermis. (2) From the third day to the ninth day after transplantation of the upper part of the rat hair follicle, the upper fragment of the hair follicle was observed to be growing downward. The complete follicles were re-formed. Wnt 5A was strongly expressed in the regeneration related tissues. (3) The high expression of Wnt5a in the site of dermal papilla formation in this model and dermal papilla embryogenesis suggests that Wnt signaling pathway is closely related to the regeneration of dermal papilla, and Wnt5a may play a very important role. [ABSTRACT FROM AUTHOR]

BACKGROUND: Noggin is an inhibitory molecule of bone morphogenetic protein (BMP) signaling, which can bind to BMP2/4 to form a complex, thereby blocking BMP signaling and affecting normal development. Some studies have suggested that dermal sheath cells are a kind of dermal stem cells that can self-renew for a long time, and participate in the formation of dermal papilla and dermal sheath during hair follicle regeneration. During the development of hair follicles, Noggin is involved in the induction of hair follicle regeneration, and its loss leads to a decrease in hair follicle number and slows hair follicle growth. However, little is known about the Noggin in the study of hair follicle dermal sheaths. OBJECTIVE: To study the biological function of Noggin protein in the dermal sheath of hair follicles. METHODS: The dermal sheath specific α-SMA-Cre ERT2 tool mice were used to specifically overexpress Noggin protein in the dermal sheath. At 8 and 9 days after birth, αSMA-CreER; pMES-Noggin mice in the experimental group and αSMA-CreER mice in the control group were injected with 4-hydroxytamoxifen. At 21, 23, and 28 days after birth, mouse skin tissues were obtained. Hematoxylin-eosin staining was used to observe hair follicle growth and subcutaneous fat layer thickness, and immunohistochemical staining was used to analyze cell phenotype. RESULTS AND CONCLUSION: Based on the findings of hematoxylin-eosin staining, we found that the growth of hair follicle was not affected, but the growth and development of subcutaneous adipose tissue of hair follicles were seriously impacted, presenting with the subcutaneous fat layer becoming thinned. The immunohistochemical results indicated that the decrease of BMP signaling may result in the thinning of the hair follicle fat layer. This discovery expands the understanding of dermal sheath cells and Noggin protein, and provides an important basis for more accurate understanding of hair follicle regeneration and tissue growth mechanisms. [ABSTRACT FROM AUTHOR]

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the effect of Angelica Sinensis on the model of alopecia areata induced by sodium sulfide and its possible mechanism. Methods: Fifty male KM mice were randomly divided into control group, model group and Angelica Sinensis high, medium and low dose groups, with 10 rats in each group. The model group and the high, medium and low doses of Angelica sinensis were locally coated with 8% sodium sulfide alcohol solution to establish alopecia areata model. The high, medium and low doses of Angelica sinensis were given different doses of Angelica sinensis. The blank control group and the model group were given. The same volume of normal saline was administered once a day for 7 days. The levels of serum interleukin-10 (IL-10) and the number of follicles per unit of field were measured by enzyme-linked immunosorbent assay (ELISA) and hematoxylin-eusin (HE) staining, respectively. The growth was scored. Results: The hair growth score, the number of hair follicles per unit field and the serum IL-10 level in the model group were significantly lower than those in the blank control group (P<0.05). Compared with the model group, the hair growth score, the number of percutaneous hair follicles and the serum IL-10 level in the high, middle and low dose groups were significantly higher than those in the model group(P<0.05). The hair growth score of the medium dose group was significantly higher than that of the high and low dose groups(P<0.05). There was no significant difference between the low dose group and the high dose group, the hair growth score, the number of percutaneous hair follicles and the serum IL-10 level (P>0.05).Conclusions: Oral Angelica has a germinating effect on sodium sulfide-induced alopecia areal mouse model, and the best effect is moderate dose. The growth of hairy root may be related to the increase of serum IL-10 level. [ABSTRACT FROM AUTHOR]

Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Imaging Science & Photochemistry is the property of Imaging Science & Photochemistry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To observe the effect of the carboxymethyl chitosan (CMC) in promoting the regenerative repair of mice skin hair follicle and compare with the tissue morphology of newborn mice skin. Methods: First to establish the mice model of full-thickness skin defects. Then the mice models were divided into 4 groups: CMC group, Qing dejia group, NaCl group, and the blank Control group. There were 10 mice in each group. Except the mice in blank Control group, medicines were given to the mice in the other three groups accordingly until 21 day. Thirty-six newborn mice were also included into the study. The repair rate of the mice model with skin hair follicle and the development of the newborn mice skin were measured with the Image J technique. The development of the skin hair follicle was observed with HE staining technique. The regeneration repaired morphological structures of the mice models executed in the 21 day in the four groups were compared with the skin tissue morphology of newborn mice. Results: The regenerative repair rates of the skin defect at 3 and 17 day were 33.07± 9.52%and 96.75± 2.11%in CMC group, 24.18± 6.14%and 92.22± 0.62%in Qingdejia group, 21.06± 11.59% and 87.38± 2.79% in NaCl group, and 8.66± 2.27% and 85.15± 1.34% in blank Control group respectively. Comparing the rates in CMC group with those in the other three groups respectively, significant differences were found（F=11.970, 17.666, 8.828, P＜0.05）. The development of skin thickness and the hair follicle number increase with the growth day age of newborn mice（r=0.983, 0.922, P＜0.05）. Compared with the skin tissue morphology of newborn mice, the structure of the regeneration repaired skin at 21 day in CMC group, Qingdejia group, NaCL group and blank Control group equalized the late-stage of development, the mid-stage of development, the early-stage of development and the stage of scar respectively. Conclusion: CMC may play an important role in promoting skin regenerative repair. [ABSTRACT FROM AUTHOR]

Objective To investigate the mitochondrial DNA (mtDNA) mutation load in hair follicles from different scalp regions of patients with mitochondrial, encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS), compare the mutation load in different scalp regions, and analyze the difference in mutation load between scalp regions with lesion site in the brain and scalp regions without lesion site in the brain. Methods Seven MELAS patients with m.3243A > G mutations were studied. Hair follicles were obtained from 8 scalp regions (bilateral frontal, temporal, parietal and occipital lobes) of all patients and DNA was extracted. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to detect the m.3243A > G mutation. Results The mean value of m.3243A > G mutation load in hair follicles from all patients was (60.57 ± 7.71)%. In different scalp regions, the mean mutation load was (61.30 ± 7.32)% in left frontal, (65.41 ± 5.85)% in right frontal, (59.80 ± 5.58)% in left temporal, (57.59 ± 14.47)% in right temporal, (62.46 ± 5.02)% in left parietal, (60.11 ± 7.11)% in right parietal, (59.70 ± 8.68)% in left occipital and (59.42 ± 6.28)% in right occipital regions, respectively. There was no significant difference in the m.3243A > G mutation load among different scalp regions (F = 0.537, P = 0.802). There was no significant difference in the mutation load between scalp regions corresponding lesion site of the brain and scalp regions incorresponding lesion site of the brain [(60.33 ± 8.70)% vs. (61.02 ± 6.52)%; t = 0.319, P = 0.751)]. Conclusions Hair follicles are convenient and noninvasive sampled tissue for detecting mtDNA mutations. There is no difference in the mutation load among different scalp regions. Furthermore, there is no difference between scalp regions corresponding lesion site of the brain and scalp regions incorresponding lesion site of the brain. [ABSTRACT FROM AUTHOR]

BACKGROUND: Skin-derived precursor cells play a regenerative role in wound healing, which can restore the physiological and aesthetic features of the skin by mitosis, proliferation and migration to the injury region. OBJECTIVE: To observe the structure of the mouse tail and the healing of the broken end, and then to investigate the correlation of these characteristics with the repair of skin trauma at the tail. METHODS: The tails of 2-day-old Kunming mice were cut off using ophthalmic microsurgical scissors to establish the mouse model of hair follicle regeneration in the tail. Regions in which the cells proliferated actively were determined. Wound healing samples were regularly taken, and embedded using OCT. Wound healing of the broken end was routinely observed by pathological staining. Cell proliferation was observed by immunofluorescence. Expression of AP-1 in the healing site was identified by immunofluorescent staining. RESULTS AND CONCLUSION: The structure of the mouse tail was complex, with rich blood supply. Good healing was observed in the broken end of the mouse tail. The cells in the hair follicles and dermal papillary layer showed active proliferation and mitosis, and thereby participated in wound repair and regeneration. Fluorescence staining results found that the positive expression of AP-1 was mainly concentrated in the epidermis and dermal papilla. These findings indicate that the epidermis and dermal papilla possess a strong ability to regenerate. [ABSTRACT FROM AUTHOR]