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[Objective] The present paper aimed to explore the effects of environmental factors on bacterial community structure in rhizosphere soil of wild Gymnadenia conopsea in Tibet. (Method) Therefore, four sampling sites were selected to collect soil samples, including the abandoned timber factory (FM), Linzhi viewing station (LZ), Linhai viewing station (LH), and the radar station (LD), and the high-throughput sequencing technology was used to analyze the diversity and structure of bacterial community in rhizosphere soil of G. conopsea at different altitudes, and the effects of different environmental factors on the diversity and structure of bacterial community were analyzed. [Result] The total of 32 phyla, 90 classes, 224 orders, 378 families, and 693 genera were detected in all samples. Proteobacteria, Actinobacteria and Acidobacteria were the dominant phyla, Bradyrhizobium and Chryseobacterium were the dominant genera. With the increase of altitude, the diversity and richness of rhizosphere soil bacterial community increased, and the difference between groups was greater than the difference within groups. [Conclusion] Altitude, TK, pH and EC significantly affected the bacterial community composition in rhizosphere soil at phylum level. Three environmental factors, namely TK, TP and altitude, had more obvious effects on bacterial community composition at genus level. Key words: Gymnadenia conopsea; Environmental factor; Rhizosphere soil bacteria; Community diversity; Community structure [ABSTRACT FROM AUTHOR]

[Objective] The study aimed to analyze the diversity of fungi community in the rhizosphere soil of Gymnadenia conopsea and its correlation with soil environmental factors, so as to provide reference for exploring the work of resource protection and sustainable utilization such as artificial cultivation and ecological planting of G. conopsea. [Method] High-flux sequencing and comparative analysis were carried out on the diversity of rhizosphere soil fungal communities of G. conopsea in Aruhorqin Banner, Dongwuzhumuqin Banner and Ongniud Banner of Inner Mongolia by using Illumina Miseq high-throughput sequencing technology, and the physical and chemical properties and correlation of plant rhizosphere soil were analyzed. [Result] The rhizosphere soil of G. conopsea was acidic, rich in organic matter and available potassium, but low in nitrogen and phosphorus, and the soil physical and chemical properties in different distribution areas were significantly different. A total of 5125 OTUs were obtained from soil samples from three distribution areas, belonging to 11 phyla, 31 classes, 65 orders, 161 families and 158 genera. At the phylum level, the dominant phyla of fungi in the rhizosphere soil of the three distribution areas were Ascomycota and Basidiomycota. At the genus level, Trichoglossum, Hygrocybe and Mortierella were the dominant groups of G. conopsea rhizosphere soil fungi. Alpha diversity index showed that the general trend of diversity and richness of rhizosphere soil fungal community in G. conopsea in the three distribution areas was Al > D3 > W2; Beta diversity index showed that there were some differences in the composition of true communities in rhizosphere soil of G. conopsea in the three distribution areas. The contents of total potassium, total phosphorus, total nitrogen and water in the soil may be the key factors affecting the composition of the fungal community in the rhizosphere soil of G. conopsea distributed in Inner Mongolia. Among them, Hygrocybe and Clavulinosis had significant positive correlation with the key factors of soil environment; However, there was a significantly negative correlation between the key factors of soil environment and the pathogenic species of Hyphomycea and Fusarium. [Conclusion] Through the results of the study, we have a preliminary understanding of the growth soil conditions and characteristics of the rhizosphere soil fungal community of G. conopsea distributed in Inner Mongolia. Among the three different distribution areas, the diversity and richness of the rhizosphere soil fungal community of G. conopsea in Aruhorqin Banner are the highest. Soil physical and chemical properties have a great impact on the diversity of fungi in the rhizosphere soil of G. conopsea, and other influencing factors need to be further studied. [ABSTRACT FROM AUTHOR]

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