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Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】The causative agent was isolated from the diseased Micropterus salmoides tissue. The physiological properties of the pathogenic bacteria were identified, and their virulence genes and drug resistance genes were analyzed, with an aim to provide guidance for the scientific use of drugs in clinical practice.【Method】Samples were collected from pathologic tissues of diseased M. salmoides from a fish breeding factory in Zhanjiang, Guangdong Province, China. The bacteria were isolated by dilution coating method, the 16S rRNA sequence comparison and biochemical tests were performed to identify the species, and the virulence genes, drug resistance genes and drug sensitivity characteristics were also identified. 【Result】Bacteria with round colony morphology, smooth milky white surface and opaque color were isolated from pathologic tissues of M. salmoides, and numbered as MSB-2, which was identified as Aeromonas in Gram-negative bacteria through phenotypic identification by Gram staining and physiological and biochemical reactions. By amplifying the 16S rRNA gene sequence and constructing a phylogenetic tree, the bacterium was further identified as A. veronii. The strain had virulencerelated genes such as act, aer and aexT and resistance-related genes such as tetC, tetA and sull. The results of the drug sensitivity test showed that the bacterium was sensitive to carbenicillin, doxycycline, cefalexin, neomycin, gentamicin, cefradine, minocycline, cefazolin and etc. Artificial regression of healthy M. salmoides infected with A. veronii showing clinical signs similar to those of diseased fish, which were the same as those of the naturally occurring disease, such as congestion of the ventral and pectoral fin bases, redness and swelling of the anus, and obvious enlargement of the abdomen, etc. And a large amount of fluid in the abdominal cavity was found by autopsy.【Conclusion】The above results indicate that A. veronii is the pathogenic bacterium causing this disease of M. salmoides, and the approved fishery drugs such as neomycin and doxycycline can be used to control the disease during the breeding process. [ABSTRACT FROM AUTHOR]

【Objective】In recent years, probiotics have been widely used in aquaculture, but the probiotics derived from Trachinotus ovatus been rarely reported. In the study, a strain was isolated from the intestine of Trachinotus ovatus and its biological characteristics were analyzed, which provided a theoretical basis its application in T. ovatus culture.【Method】 The bacteria were isolated and identified through the sterile collection of intestinal contents from the healthy T. ovatus. And the morphological observation, gram staining, biochemical identification, 16S rRNA gene sequence analysis, drug sensitivity test, salt tolerance test, acid production ability, growth curve determination, and virulence gene identification of the typical bacterial colonies were performed.【Result】Based on the morphological characteristics, gram staining results biochemical characteristics, and 16S rRNA gene sequence alignment of the isolated bacterium, the results showed that it shared 100% similarities with Enterococcus faecalis, therefore, the isolated bacterium was identified as E. faecalis. The colony of the isolated strain was light grayish white, and Gram-positive. The biochemical indicators including esculin, cellobios, maltose, mannose and salicylic acid were tested positive. The results of the drug sensitivity test showed that the isolated bacterium exhibited varying degrees of resistance to drugs such as piperacillin, polymyxin B, penicillin, trimethoprim-sulfamethoxazole and oxacillin, exhibited intermediate sensitivity to ciprofloxacin, medemycin and ceftriaxone and sensitive to 11 drugs such as vancomycin, cefoperazone, ofloxacin and etc. The results of the salt tolerance test showed that the isolated strain could grow at 0%-8% NaCl concentrations, showing salt tolerance to some extent. The results of acid production capacity and growth curve measurements indicated that the pH of the culture media rapidly decreased and the acid production capacity was enhanced as the proliferation of the strain. The identification results of virulence genes showed that the isolated strains possessed four virulence genes including efaA, agg, as and fsrA.【Conclusion】A potential probiotic strain was successfully isolated from the intestine of T. ovatus and identified as E. faecalis. The isolated strain has good salt tolerance and acid production capability, and it contains functional genes associated with intestinal adhesion, which will provide a theoretical basis for further application of the strain. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Sichuan University (Medical Science Edition) is the property of Editorial Board of Journal of Sichuan University (Medical Sciences) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: To probe into the clinical efficacy of Kunkang powder in the treatment of cervical persistent high-risk human papilloma virus (HR-HPV) infection. METHODS: A totao of 114 patients with cervical persistent HR-HPV admitted into this hospital from Jul. 2019 to Jun. 2021 were selected to be divided into the control group and observation group via simple randomized block, with 57 cases in each group. The control group was given conventional western treatment, while the observation group received Kunkang powder on the basis of the control group, both groups were treated for 4 menstrual cycles. Patients in both groups were underwent HR-HPV and liquidbased thin-layer cytology ( TCT) examination of cervical secretions, TCM syndrome assessment, vaginal flora status and immune indicators detection [T-lymphocyte subpopulations CD3 + and CD4 +, immune cell regulators interleukin (IL)2 and IL-6] before and after treatment, the incidence of adverse drug reactions during treatment was recorded. Both groups were followed up for 1 year, and the 1-year relapse rate was counted. RESULTS: The clinical total effective rate of the observation group was 87. 72% ( 50 / 57), higher than that of the control group ( 71. 93%, 41 / 57), with statistically significant difference (P<0. 05). After treatment, the normal rate of TCT results of both groups was higher than that before treatment, and that of the observation group was higher than the control group, with statistically significant difference (P<0. 05). After treatment, the TCM syndrome integral of both groups were lower than that before treatment, and that of the observation group was lower than the control group, with statistically significant difference (P<0. 05). After treatment, the pH value of vaginal secretions and nugent Gram staining score decreased significantly in both groups (P<0. 05); the pH value of vaginal secretions and nugent Gram staining score in the observation group were lower than those in the control group (P<0. 05), and the recovery rate of vaginal flora of the observation group was higher than those of the control group (P<0. 05), with statistically significant differences. After treatment, the CD3 +, CD4 + and IL-2 levels of both groups were higher than those before treatment (P<0. 05), while the IL-6 level were lower than those before treatment ( P < 0. 05); the CD3 +, CD4 + and IL-2 levels of the observation group were higher than those of the control group (P<0. 05), and the IL-6 level of the observation group was lower than that of the control group (P<0. 05), with statistically significant differences. There was no statistical significance in differences in incidence of adverse drug reactions and 1-year recurrence rate between observation group and control group (P>0. 05). CONCLUSIONS: Kunkang powder can regulate the immune function of patients with cervical persistent HR-HPV infection, improve the HR-HPV negative conversion rate and alleviate the TCM syndrome, which has good clinical efficacy and treatment safety. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective] Glaesserella parasuis (GPS) is the pathogen that causes Glässer’s disease in pigs. It is one of the most serious bacterial pathogens that harm pig industry. There are many serotypes of this bacterium, and the crossprotection between different serotypes is weak. Serotype 2 is a moderately virulent strain, and the proportion of clinical isolates has been increasing, resulting in increasingly serious harm in recent years. GPS serotype 2 was isolated from nursery pigs in a large-scale pig farm in Henan Province and a series of tests were conducted, and provided reference for scientific prevention and control of the disease. [Method] In this study, lung, trachea, blood and other samples of GPS clinically suspected cases pigs were collected. Bacterial isolation and purification, Gram staining microscopy, routine PCR, guinea pig pathogenicity test, AGAR diffusion drug sensitivity test and other methods were used. Morphological observation, identification, serotyping, virulence gene detection, drug-resistant gene detection, pathogenicity and drug resistance of suspected bacteria were studied. [Result] A strain of GPS was isolated from lung samples and identified as serotype 2. The strain carried virulence genes of vta1, vta2, vta3, wza, ompP2, nanH, cdtA, cdtB, cdtC and espP2, and showed strong virulence to guinea pigs. The strain carried drug-resistant genes aadA1, strA, strB, aphA1, tet(B), sul2, and had strong resistance to penicillin G, kanamycin, amicacin, streptomycin, tetracycline, oxytetracycin, and cotrimoxazole. It had good sensitivity to ceftiofurme, ceftazidime, amoxicillin, ampicillin, levfloxacin, ofloxacin, ennofloxacin and ciprofloxacin, and had significant clinical effect. [Conclusion] The isolates of GPS serotype 2 have strong virulence, can pass through the blood-brain barrier, and have obvious multi-drug resistance. The results of this study provide reference for the epidemiological investigation of GPS serotype 2, pathogenic mechanism, and the formulation of clinical prevention and control measures. [ABSTRACT FROM AUTHOR]

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The study was to establish a method for the detection of Clostridium butyricum. In the test, the purified clostridium butyricum powder was diluted and coated in four different media, including modified FTG, FTG, TSC and TSN, to screen the appropriate medium, and the temperature and pH value gradient were set to screen the most appropriate conditions. Then, the detection method of Clostridium butyricum was verified by gram staining microscopy, biochemical identification, PCR and sequencing identification. The results showed that an improved FTG medium with low cost, strong specificity and easy to observe and count Clostridium butyricum was selected by observing morphology and colony count. The optimum culture conditions were pH (7.0±0.2) and temperature (37±1) ℃. The detected bacteria were grampositive, rod-shaped or spindle shaped, terminal or secondary spores, and positive for lactose, salicin, xylose, starch, melibiose and raffinose. Both the sample and the positive control of Clostridium butyricum showed a band at 548 bp, and the sequence homology was more than 96%, indicating that the detection result was accurate. The study indicates that an improved FTG medium with strong specificity and easy to observe and count Clostridium butyricum is screened, which can effectively detect the content of Clostridium butyricum. [ABSTRACT FROM AUTHOR]

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】The present paper aimed to obstain Bacillus mucilaginosus from the rhizosphere soil of Luoyang tobacco plant and improve the content of available free potassium of plants in soil. 【Method】B. mucilaginosus in soil was identified by plate dilution coating, gram staining, physiological and biochemical detection and 16 S rDNA sequence analysis, and the potassium solubility of the strain was compared with that of the standard strain by sodium tetraphenylborate precipitation method. 【Result】Nine single colonies, which were round, white and transparent, with neat edges and smooth protruding, were isolated from the root soil samples of tobacco fields at different growth stages in three counties of Henan province. Which the physiological and biochemical characters of strain 005, 008 and standard strains, were facultative anaerobic bacteria, and hydrogen peroxide enzyme reaction, also can make the starch hydrolysis, sugar alcohol fermentation, nitrate reduction, in the determination of V-P with M. R results have shown positive, in litmus milk test reaction can produce acid reaction, cannot make gelatin liquefaction, which can use citrate. The 16 S rDNA sequences of strain 005（MN227334） and strain 008（MN227337） were 99% homologous to GenBank（EU048557）. It was identified as B. mucilaginosus. The results showed that the precipitation weight of strain 005, 008 and standard strain 23575 was 0. 140, 0. 136 and 0. 166 g, respectively, with a significant difference of 0. 05, which were all 0. 040 g higher than that of the blank group. 【Conclusion】In the rhizosphere soil of Luoyang tobacco plant, there are round, white and transparent facultative anaerobic bacillus coleformis, which can hydrolyze starch, ferment sugar alcohol, reduce nitrate, and have strong ability to release potassium. [ABSTRACT FROM AUTHOR]

Objective: To observe the pathogenic bacteria distribution of wound infection in patients with deep burn of limbs, and to analyze the risk factors of infection and the clinical significance of the changes of serum proinflammatory factors levels. Methods: The clinical data of 220 patients with deep burn of limbs who were admitted to the Department of Burn and Plastic Surgery, Hainan Third People’s Hospital from March 2018 to January 2021 were retrospectively analyzed. According to whether wound infection occurred, they were divided into infected group and non-infected group. The distribution characteristics of pathogenic bacteria in wound secretion of infected group were observed, and relevant data were collected. To analyze the risk factors of wound infection, and the levels of serum proinflammatory factors in infected group and uninfected group were compared. Results: Among 220 patients with deep burn of limbs,64 cases had wound infection, and the infection rate was 29.09%. A total of 102 strains of pathogenic bacteria were detected from 64 infected patients. Gram negative bacteria accounted for 67.65% (69/102), among which Pseudomonas aeruginosa (52.94%), Acinetobacter baumannii (7.84%) and Escherichia coli (2.94%) ranked among the top three. Gram positive bacteria accounted for 32.35% (33/102),among which Staphylococcus aureus (14.71%) and Staphylococcus epidermidis (7.84%) were the top two. No fungi were detected. Univariate analysis showed that wound infection in patients with deep burn wounds was associated with postoperative residual wounds, whether diabetes mellitus, burn area, inhalation injury, length of stay and burn depth (P<0.05), but not related to age, gender, burn site and burns reason (P>0.05) . Multivariate Logistic regression analysis showed that residual wound, complicated with diabetes, burn area of more than 40%, inhalation injury and burn depth of Ⅲ degrees were all risk factors for wound infection in patients with deep burn wounds after operation (P<0.05). Serum high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) level in the infection group were higher than those in the uninfected group (P<0.05) . Conclusion: The incidence of wound infection in patients with deep burn of limbs is high, and the incidence of infection is related to many factors. The pathogenic bacteria of wound infection are pseudomonas aeruginosa and Staphylococcus aureus, so early intervention and appropriate antibiotics should be used in clinic. [ABSTRACT FROM AUTHOR]

Objective: To investigate the differential role of procalcitonin (PCT), interleukin-6 (IL-6), C-reactive protein (CRP) and neutrophil/lymphocyte ratio (NLR) in Gram-positive bacteria (G+bacteria) and Gram-negative bacteria (G-bacteria) in patients with bacterial bloodstream infection (BSI) in intensive care unit (ICU) and its predictive value for the risk of death. Methods: 99 patients with bacterial BSI who were treated in our hospital from February 2019 to December 2021 were selected, and they were divided into G-bacteria infection group and G+bacteria infection group according to the identification results of bacterial Gram staining culture. The levels of PCT, IL-6, CRP and NLR in the two groups were detected and compared, and the differential value of the above indexes for G+bacteria and G-bacteria infection was analyzed according to the receiver operating characteristic (ROC) curve. The difference of levels of PCT, IL-6, CRP and NLR of different pathogen types of G+bacteria and G-bacteria were compared. In addition, all patients were divided into death group and survival group according to the 28 d prognosis difference. The levels of PCT, IL-6, CRP and NLR in the two groups were compared. The predictive value of the above indexes for the death of patients with bacterial BSI in ICU was analyzed by ROC curve. Results: (1) Among 99 patients with bacterial BSI, the pathogen type were G-bacteria with 70 cases (70.71%), and G+bacteria with 29 cases (29.29%). (2) The levels of PCT and NLR in G-bacteria infection group were higher than those in G+bacteria infection group (P＜0.05), but there was no significant difference in the levels of IL-6 and CRP between the two groups (P>0.05). The ROC-AUC (0.95CI) of combined detection of levels of PCT and NLR to identify G+bacteria and G-bacteria was 0.855 (0.770～0.912), the identification efficiency was good. (3) The levels of PCT, IL-6 and CRP of Klebsiella pneumoniae were higher than those of Escherichia coli, Acinetobac ter baumannii, Pseudomonas aeruginosa and other G-bacteria, while the level of NLR was lower than that of Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa and other G-bacteria (P<0.05). The levels of PCT, IL-6, CRP and NLR in Streptococcus were higher than those in coagulase negative staphylococcus, Staphylococcus aureus and Enterococcus (P<0.05). (4) The levels of PCT, IL-6 and NLR in the death group were higher than those in the survival group (P<0.05), but there was no significant difference in the level of CRP between the two groups(P>0.05). The ROC-AUC (0.95CI) of combined detection of the levels of PCT, IL-6 and NLR to predict the death of patients with bacterial BSI in ICU was 0.871 (0.787～0.937), the prediction efficiency was good. Conclusion: The combined detection of PCT and NLR has a certain value in identifying G-bacteria and G+bacteria in patients with bacterial BSI in ICU, while the combined detection of PCT, IL-6 and NLR has a certain predictive value for the risk of death. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The study was to isolate and screen lactic acid bacteria with potential probiotics from feces of healthy canine. In the study, 30 strains isolated from the feces of healthy canine were identified by Gram staining, colony morphology observation and 16S rDNA sequence analysis, and the functional evaluation of bacteriostatic ability, antibiotic sensitivity, acid tolerance and bile salt tolerance, cell adhesion ability were carried out. The best strains were selected for animal experiment. The results showed that nine strains of Lactobacillus had good bacteriostatic effect on Escherichia coli, Shigella, Salmonella and Campylobacter jejuni, among which strain J40 had the strongest bacteriostatic effect. Antibiotic sensitivity tests showed that nine lactic acid bacteria strains were highly sensitive to penicillin, ampicillin and cephalothiophene antibiotics, which proved that the nine strains did not carry the drug resistance genes, which had certain safety and controllable. The survival rate of four strains cultured at pH value 2 and bile salt concentration 0.1% for 2 h were all no less than 50%, showing good acid tolerance and bile salt tolerance. The number of adhesion to Caco-2 cells by four strains above was all more than six, and J40 had the best adhesion performance. J40 was selected as the treatment group to conduct in vivo experiments on Shigella challenged puppies. The results showed that strain J40 could effectively reduce the rate of diarrhea in puppies, treat bacterial diarrhea. The experiment indicates that J40 can be used as a potential probiotic strain for pet microecologics. [ABSTRACT FROM AUTHOR]

The experiment was to screen strains that could synthesize and secrete methionine from bovine rumen microorganisms. The rumen fluid of grassland red cattle was isolated and purified with methionine deficient medium. The strains were identified by Gram staining, determination of free methionine content and 16S rDNA sequencing. The culture medium and fermentation conditions were optimized by single factor design and orthogonal test. The strain was tested by in vitro rumen. The results showed that the isolated strain was Candida tropical TYM001. The optimum carbon source was 20.0 g/L of glucose, the optimum nitrogen sourcewas 10.0 g/L of amino acid free yeast, and the optimum inorganic salts 5.0 g/L of NaCl, 7.0 g/L of MgSOq and 5.0 g/L KH2PO4. The content of free methionine reached 27.815 mg/L under the best suitable 宦rmentation condition. The experiment indicates that the strain can improve the content of total protein and free methionine in simulated rumen in vitro, providing reference for the development of ruminant microbial additives. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] This paper aimed to isolate and identify the pathogen species of Pseudobagrus fulvidraco in Tongwang farm of Han chuan city, Hubei province, and the characteristics of its effects on the expression of antimicrobial peptide gene in host were analyzed, so as to provide a research basis for the prevention and treatment of the disease. [Method] The pathogen PA-5 from the liver of diseased Pel teobagrus fulvidraco by line culture was isolated. Gram staining and microscopic observation showed that the morphology of the pathogen was rod-shaped and gram-negative. The pathogen was sensitive to d-Gluconic acid, malonic acid and glucose, but insensitive to myxin, aescin and arabinose. The 16S rRNA and gyrB gene sequences of the strain were analyzed by multiplex PCR. [Result] It was found that the charac teristic sequence of the strain was similar to that of P. aeruginosa, the genetic relationship was close, and after synthesis, the pathogen was identified as P. aeruginosa. At the same time, the expression of antimicrobial peptide gene PCl / PC2 / PC3 in the host was analyzed after the pathogen was infected with P.fulvidraco. P. aeruginosa PA5 ( 3. 0 x 106 CFU/ mL) was injected into the abdominal cavity of P. fulvidraco, and the liver, kidney and epidermis of P. fulvidraco were dissected 0 - 96 hours after infection. Then the total RNA of P. fulvidraco was obtained by Trizol kit, the cDNA was synthesized by reverse transcription, and the expres sion characteristics of antibacterial peptide gene PCl / PC2 / PC3 gene in P. fulvidraco was analyzed by real-time fluorescent quantitative PCR. After P. aeruginosa PA-5 infected P. fulvidraco, the rela tive expression of antimicrobial peptide gene PCl / PC2 / PC3 in liver tissue was higher than that in control group at 24 and 36 hours ( P < 0. 05) ; The relative expression level of antimicrobial peptide gene PCl / PC2 / PC3 in host kidney tissue was higher than that in control group at 24 and 36 hours (P < 0.05); The relative expression of antimicrobial peptide gene PCl / PC2 / PC3 in host epidermis was 36, 48 hours was higher than that in the control group ( P < 0. 05) . The expression of antimicrobial peptide gene in liver, kidney and epidermis increased rapidly, but the reaction time of liver and kidney was earlier than that of epidermis. [Conclusion] Therefore, P. aeruginosa PA-5 was the main pathogen of P. fulvidraco in Tongwang farm of Hanchuan city, Hubei province. The host antimicrobial peptide gene PC1/PC2/ PC3 would respond positively to the infection of P. aeruginosa in Tongwang farm of Hanchuan city, Hubei province, which were conductive to antimicrobial infection. [ABSTRACT FROM AUTHOR]

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective:To explore the relationship between the expression of receptor for activated C kinase 1 (RACK-1) and leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) and clinicopathological features and prognosis in cervical cancer tissues (CC). Methods: 134 patients with CC who underwent surgical treatment in Affiliated Renhe Hospital of China Three Gorges University from January 2012 to January 2016 were selected as the study subjects. CC tissues and corresponding normal tissues adjacent to cancer were selected, and normal cervical tissues of 134 patients with benign lesions or attachment resection of benign lesions in the hospital as a control. Immunohistochemical streptomycin antibiotic protein-peroxidase linkage (SP) was used to stain various tissues. The expression of RAC K-1 and Lgr5 in CC tissues was analyzed according to the positive cell rate and staining intensity, and the correlation between the expression of RACK-1 and Lgr5 and the clinicopathological characteristics of patients with CC was analyzed. Prognosis of patients with CC with different positive expression intensity of Lgr5 and RACK-1. Results: The positive expression rates of RACK-1 and Lgr5 protein in cervical cancer tissues were higher than those in adjacent tissues and normal cervical tissues, the differences were statistically significant (P<0.05). The positive expression of RACK-1 and Lgr5 were closely related to TNM stage, tumor differentiation, lymph node metastasis, intravascular tumor thrombus (P<0.05), and they were not with the age, tumor size, pathological type, depth of tumor invasion, parametrial invasion, surgery cutting edge (P>0.05). The 3-year survival rate and survival time of patients with positive high expression of Lgr5 and RACK-1 were lower than those of patients with positive low expression of Lgr5 and RACK-1, the differences were statistically significant (P<0.05). Conclusion: The expression of RACK-1 and Lgr5 are up-regulated in CC tissues, which are related to some clinicopathological features and prognosis. Detection of RACK-1 and Lgr5 is helpful to the diagnosis and prognosis of patients with CC. [ABSTRACT FROM AUTHOR]

Objective: To explore the relationship between the expression of receptor for activated C kinase 1 (RACK-1) and leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) and clinicopathological features and prognosis in cervical cancer tissues (CC). Methods: 134 patients with CC who underwent surgical treatment in Affiliated Renhe Hospital of China Three Gorges University from January 2012 to January 2016 were selected as the study subjects. CC tissues and corresponding normal tissues adjacent to cancer were selected, and normal cervical tissues of 134 patients with benign lesions or attachment resection of benign lesions in the hospital as a control. Immunohistochemical streptomycin antibiotic protein-peroxidase linkage (SP) was used to stain various tissues. The expression of RAC K-1 and Lgr5 in CC tissues was analyzed according to the positive cell rate and staining intensity, and the correlation between the expression of RACK-1 and Lgr5 and the clinicopathological characteristics of patients with CC was analyzed. Prognosis of patients with CC with different positive expression intensity of Lgr5 and RACK-1. Results: The positive expression rates of RACK-1 and Lgr5 protein in cervical cancer tissues were higher than those in adjacent tissues and normal cervical tissues, the differences were statistically significant (P<0.05). The positive expression of RACK-1 and Lgr5 were closely related to TNM stage, tumor differentiation, lymph node metastasis, intravascular tumor thrombus (P<0.05), and they were not with the age, tumor size, pathological type, depth of tumor invasion, parametrial invasion, surgery cutting edge (P>0.05). The 3-year survival rate and survival time of patients with positive high expression of Lgr5 and RACK-1 were lower than those of patients with positive low expression of Lgr5 and RACK-1, the differences were statistically significant (P<0.05). Conclusion: The expression of RACK-1 and Lgr5 are up-regulated in CC tissues, which are related to some clinicopathological features and prognosis. Detection of RACK-1 and Lgr5 is helpful to the diagnosis and prognosis of patients with CC. [ABSTRACT FROM AUTHOR]

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