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Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The high sugar content of Huanghua winter jujube brings delicious taste, but at the same time makes its chips easy to absorb moisture during storage, resulting in softening of the chips and obviously affecting the taste of them.In this paper, glucose oxidase was used to partially remove sugar from winter jujube chips.The orthogonal optimization method was adopted to study the effects of enzymolysis time, enzyme addition amount, enzymolysis temperature, solid-liquid ratio and thickness of winter jujube chips on the desugarization rate and sensory quality of winter jujube chips.Isothermal adsorption method was also used to study the storage stability of winter jujube chips.The results showed that when the enzymolysis time was 35 min, the enzyme dosage was 0.15%, the enzymolysis temperature was 45 °C, the ratio of solid to liquid was 1:2(g/mL), and the thickness of winter jujube chips was 10 mm, the desugar rate was 43.30%, that is, the reducing sugar content of winter jujube chips was 18.14%.This content was significantly lower than that before desugar removal (32.00%), with the sensory score being 85.86 points, and the comprehensive score being 98.98 points.Under this condition, the desugar rate was the highest, or the reducing sugar content decreased to the lowest, the comprehensive score reached the highest, and the moisture absorption performance of winter jujube chips became weaker and the stability was better.The research results will provide some theoretical and data support for the development of winter jujube chips related products. [ABSTRACT FROM AUTHOR]

The experiment aims to use flocculation technology for solid-liquid separation of the fermentation broth of Pichia pastoris. With the flocculated cell rate and enzyme activity recovery rate as the measurement indicators, singlefactor experiments and orthogonal experiments were conducted to study the effects of different flocculants on the flocculation of recombinant Pichia pastoris expressing glucose oxidase in the fermentation broth. The results showed that the optimal flocculation process for the Pichia pastoris fermentation broth was to first add 2.0% polyaluminum chloride, then 1.0% diatomite, and finally 0.2% cationic polyacrylamide, ensuring that the volume of polyaluminum chloride mother liquor added did not exceed 20% of the total volume of the fermentation broth. Under these conditions, the fermentation broth's cell flocculation rate reached over 90%, and the recovery rate of glucose oxidase enzyme activity reached over 85%. The study indicates that the use of flocculation technology can achieve solid-liquid separation of the Pichia pastoris fermentation broth, a method that is beneficial for improving the production efficiency of enzymes, thereby promoting the development of the feed enzyme preparation industry. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The purpose of the study was to study the effects of different levels of glucose oxidase on the egg laying performance, immunity and antioxidant capacity of Hyline brown laying hens in the late laying period. 1 000 healthy Hyline brown laying hens were selected and randomly divided into five groups, with 10 replicates per group and 20 hens per replicate. The Hyline brown laying hens in the control group, test group 1, test group 2, test group 3, and test group 4 were fed with 0, 100, 200, 400 and 800 mg/kg of glucose oxidase. The pre-test period was seven days, and the formal test period was 56 days. The results showed that the egg laying rate and average daily egg weight of Hyline brown laying hens in the test group 2, test group 3 and, test group 4 were significantly higher than those in the control group （P<0.05）, and the feed-to-egg ratio was significantly lower than that in the control group and the test group 1 （P<0.05）. The Huff units of eggs in the test group 3 and test group 4 were significantly higher than those in the control group and test group 1 （PcO.05）. The serum levels of immunoglobulin A and immunoglobulin M in the test group 3 and test group 4 were significantly higher than those in the control group and test group 1 （P<0.05）, and the serum levels of immunoglobulin G in the test group 2, test group 3, and test group 4 were significantly higher than those in the control group （JkO.05）. The serum levels of interferon-y, interleukin-2, and interleukin-10 in the test group 2, test group 3, and test group 4 were significantly higher than those in the control group （P<0.05）. The level of tumor necrosis factor-a was significantly lower than that of the control group and the test group 1 （Jk0.05）. The activities of total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in the serum of laying henS in血teSt gr°Up 2, test group 3 and test group 4 were significantly higher than those in the control group （P<0.05）, and the serum activities of superoxide dismutase in the test group 3 and test group 4 were significantly higher than those in the group 1 (P<0.05). The serum malondialdehyde level in all glucose oxidase groups was significantly lower than that in the control group (fk0.05). The study indicates that glucose oxidase can improve egg production performance, immunity, and antioxidant function of laying hens, and the appropriate addition level of glucose oxidase is 400 mg/kg.. [ABSTRACT FROM AUTHOR]

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chemistry & Industry of Forest Products is the property of Chemistry & Industry of Forest Products Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Purposes Combining the high selectivity of natural enzymes with the high stability of nanoenzymes in a one-pot reaction has emerged as an ideal solution to improving the diversity/complexity of biocatalytic cascades and the stability of multi-enzyme systems. Methods In this paper, we designed and synthesized an ionic magnetic Fe3O4@EB-COFs material to prepare glucose oxidase (GOx)-magnetic covalent organic framework material(GOx/Fe3O4@EB-COFs) through electrostatic interaction induction. Findings Fe3O4@EB-COFs played the role of nanoparticle enzyme and carrier material, and the obtained composite could be used for glucose concentration for colorimetric determination. Under acidic conditions, GOx/Fe3O4@EB-COFs exhibited a good cascade of catalytic glucose oxidation and 2,2′-biazo-bis-3-ethylbenzothiazoline-6-sulfonic acid oxidation. The kinetic study showed that the reaction followed the Lineweaver-Burk equation, indicating that the cascade reaction of the constructed nanoenzyme with natural enzyme still exhibited biocatalytic characteristics. GOx/Fe3O4@EB-COFs exhibited good recoverability with the addition of magnetic Fe3O4. Conclusions Therefore, the construction of a multi-enzyme catalytic system of nanoenzymes and natural enzymes is important for the realization of biomimetic catalysis.

Glucose oxidase is an oxygen-consuming dehydrogenase that can catalyze the production of gluconic acid and hydrogen peroxide from glucose, which is a new type of green enzyme and plays an important role in fields of animal husbandry and food industry. Glucose oxidase can scavenge intestinal free radicals, decrease intestinal pH value and increase the abundance of intestinal beneficial bacteria, thus protect intestinal health and improve the immunity of livestock and poultry. The paper summarizes the structure, catalytic mechanism, industrial application and action mechanism of glucose oxidase, summarizes the research progress of glucose oxidase in animal production, so as to provide reference for further expanding the application range of glucose oxidase in animal production and revealing the specific mechanism of action. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Tea Science is the property of Journal of Tea Science Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Honey is a natural sweet substance made from a mixture of nectar collected by worker bees and their own secretions, which has good antibacterial activity and contains a variety of natural antibacterial active substances. In recent years, a large number of studies have been reported on the progress of antimicrobial active substances in honey, including methylglyoxal, hydrogen peroxide, polyphenols, antimicrobial peptides and Major royal jelly proteins. Numerous studies have shown that hydrogen peroxide is one of the most important antibacterial substances in honey. This paper highlights the important roles by each antimicrobial effective active substance for the anti-bacterial by honey. It also further summarizes the process of glucose oxidase catalyzing the production of hydrogen peroxide from glucose. The process of glucose oxidase catalyzing the production of hydrogen peroxide from glucose is summarized using hydrogen peroxide in honey as an example. Other pathways for the production and degradation of hydrogen peroxide and the physicochemical factors affecting the production of hydrogen peroxide are also reviewed. Rational regulation of hydrogen peroxide production in honey plays an important role in its antibacterial and even other biological activities. This review would also provide a reference for the safe clinical use of honey and the development of functional foods.

AIM: To investigate the effect of Shipi-Xiaoji Formula（ SPXJF） on ferroptosis in an in vitro model of liver cancer cells with abnormal glucose metabolism and its mechanism. METHODS: High insulin was used to treat HepG2 cells to establish a liver cancer cell model with abnormal glucose metabolism. The glucose oxidase method was used to measure glucose content, and the cell counting kit-8 （CCK-8） assay was used to evaluate the degree of inhibition to confirm the success of modeling. The cells were divided into control group, model group, metformin group, and low-, medium- and high-dose SPXJF groups. The glucose consumption, viability, proliferation, migration and invasion of the cells in each group were measured. Intracellular glutathione（ GSH）, reactive oxygen species（ ROS） and malondialdehyde （MDA） levels were measured to assess the level of lipid peroxidation. Western blot was used to detect the expression of ferroptosis-related proteins P53, solute carrier family 7 member 11 （SLC7A11/xCT） and glutathione peroxidase 4 （GPX4）. RESULTS: The results showed that glucose consumption was significantly reduced in HepG2 cells with abnormal glucose metabolism compared with normal HepG2 cells （P<0. 05）, while the cell viability remained unchanged. After treatment in each group, no significant difference in cell viability and proliferation was observed between control group and model group （P>0. 05）. However, the migration and invasion of the cells in model group were significantly higher than those of normal HepG2 cells （P<0. 05）. Metformin and SPXJF treatment significantly inhibited the viability, proliferation, migration and invasion of HepG2 cells with abnormal glucose metabolism （P<0. 05）. The GSH level was higher in model group than that in the other groups, while the ROS and MDA levels were lower. The expression of P53 was down-regulated in model group but up-regulated in metformin and SPXJF groups. The expression of xCT and GPX4 was down-regulated in model group but up-regulated in metformin and SPXJF groups（ P<0. 05）. CONCLUSION: Shipi-Xiaoji Formula induces ferroptosis in liver cancer cells with abnormal glucose metabolism through P53/xCT/GPX4 pathway. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The study was to study the effect of glucose oxidase on growth performance, serum biochemical indexes and breeding economic benefits of weaned piglets. A total of 200 weaned piglets were seclected and randomly divided into four groups, with five replicates in each group and 10 weaned piglets in each replicate. The piglets in control group were fed with basic diet, piglets in experimental group were fed with basic diet supplentment with 50, 100 and 150 mg/kg glucose oxidase. The test period lasted for 28 d. The results showed that the ADG of piglets in 100 mg/kg group and 150 mg/kg group was significantly higher than that in control group (P<0.05), F/G of piglets in 100 mg/kg group and 150 mg/kg group was significantly lower than that in control group (P<0.05), and the diarrhea rate of piglets in each experimental group was significantly lower than that in control group (P<0.05). The T-AOC and GSH-Px activity in serum of piglets in 100 mg/kg group were significantly higher than those in control group (P<0.05), the serum SOD activity of piglets in each experimental group was significantly higher than that in control group (P<0.05), and the serum MDA content of piglets in 100 mg/kg group was significantly lower than that in control group (P<0.05). The content of IgG and TNF-α in serum of piglets in 100 mg/kg group was significantly higher than that of control group (P<0.05), and the content of serum IL-6 in experimental group was significantly higher than that in control group (P<0.05). The expression of IGF-2 gene in the longissimus dorsi muscle of piglets in 100 mg/kg group and 150 mg/kg group was significantly higher than that in control group (P<0.05). The breeding profit of piglets in experimental group was 7.80, 29.28 and 16.14 yuan/head higher than that in control group. The study indicates that glucose oxidase can improve the performance and body health of piglets, and it is suggested to add 100 mg/kg glucose oxidase to diet of weaned piglets. [ABSTRACT FROM AUTHOR]

Glucose sensors as a tool for diabetics to monitor blood glucose are of great significance in the diagnosis and treatment of diabetes. With the rapid development of flexible smart wearable devices the development of glucose sensors based on flexible substrate has also gradually become a research hotspot. Silk has good biocompatibility degradability and flexibility its surface is rich in chemical bonds such as carboxyl hydroxyl and amide bonds which can interact with active substances and it is a good conductive substrate material. The flexible glucose sensor developed by using silk and silk fibroin as the substrate and by being compounded with conductive active materials has excellent sensing performance and good long-term stability. This article aims to review the research progress of silk-based glucose sensors developed based on silk fibroin silk fiber and silk fabric analyze their characteristics and mechanism and prospect their development tendency. In order to fully understand the application of silk in the field of glucose sensors this paper starts with the development of glucose sensors and focuses on the research progress of silk-based glucose sensors on basis of two main kinds of silk and silk fibroin. We first introduced the characteristics and properties of silk and discussed the application of silk yarns silk fabrics and carbonized silk fabrics in the field of glucose sensors. Then we summarized the application of fibroin films and spun fibers based on silk fibroin in the field of glucose sensors. By reviewing the preparation methods and sensing performance of various silk-based glucose sensors we analyzed the role of silk in them and summarized the effect of the silk substrate on the glucose sensing performance. It is hoped that this paper can provide a reference for the design and development of high-performance silk-based flexible glucose sensors. Glucose sensors based on silk fabrics have better mechanical properties higher conductivity and sensitivity while those made of silk fibroin have better biocompatibility and degradability. Finally we compared and analyzed the sensitivity response time detection limit advantages and disadvantages of the silk-based glucose sensors. In sum in virtue of its good characteristics silk has the excellent application value in the field of flexible glucose sensors and the developed glucose sensors show good sensing performance which can also keep the original flexibility and biocompatibility of silk and show good conductivity and ultrahigh response to glucose molecules. However the problems such as the poor fixation rate of silk fibroin to glucose oxidase the poor mechanical properties of silk fibroin films and the weak binding between silk fabric and conductive materials still need to be solved urgently. In addition the cost and maintenance of silk-based glucose sensors are also the factors that cannot be ignored. At present the development of silk-based glucose sensors is mainly based on the silk fibroin membrane and the carbonized silk fabric and there are relatively few studies and applications based on the silk fiber and the silk fabric. The functionalized silk produced by the silkworm feeding method and spinning technology can be applied to the research and development of sensors which would possibly become one of the important research directions of silk-based glucose sensors in the future. Overall although the development of silk-based glucose sensors is still in its infancy its application prospects are very broad and it is worth looking forward to future development. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of silent information regulator 2 (SIRT2)on the aerobic glycolysis, growth and proliferation of the colorectal cancer cells, and to clarify its related mechanism. Methods The HCT116 cells and SW480 cells were cultured, and Western blotting method was used to detect the expression levels of SIRT2 protein in two kinds of cells.The HCT116 cells with high expression of SIRT2 protein were selected for the following RNA interference experiment. Negative control group, SIRT2-siRNA#1 group, SIRT2-siRNA#2 group, and SIRT2-siRNA#3 group were established； the SW480 cells with low expression of SIRT2 protein were selected for the following SIRT2 overexpression experiment.The SIRT2 recombinant plasmids were constructed, and the SW480 cells at logarithmic growth phase were selected； blank group, empty vector plasmid transfection group, and SIRT2 plasmid transfection group were established；the recovery experiment was carried out in the HCT116 cells；control group, SIRT2-siRNA+glucose-6-phosphate dehydrogense (G6PD) plasmid co-transfection group and SIRT2-siRNA#3 group were established.The plasmid and siRNA were transfected into the SW480 cells and HCT116 cells by Lipofectamine 2000, respectively, the conterts of glucose in the culture medium in various groups were measured by glucose oxidase method after silencing SIRT2 and over-expression of SIRT2；the levels of lactic acid in the culture medium in various groups were measured by colorimetry after silencing SIRT2 and over-expression of SIRT2；the clone formation rates of the cells in various groups were determined by clonal formation assay； the proliferation activities of the cells in various groups were determined by CCK-8 assay；the expression levels of G6PD mRNA in the cells after silencing SIRT2 and over-expression of SIRT2 were determined by reverse transcriptionPCR (RT-PCR) method；the expression levels of G6PD protein in the cells in various groups after silencing SIRT2 and over-expression of SIRT2 were detected by Western blotting method；the expression levels of SIRT2 and G6PD proteins in colorectal cancer tissue were determined by immunohistochemistry.After co-transfection of SIRT2-siRNA and G6PD plasmids, the glucose levels, lactic acid levels, survival rates, and proliferation abilities of the cells were detected. Results Compared with negative control group, the level of glucose in the culture medium in SIRT2-siRNA#3 group was increased significantly (P<0.05), the level of lactic acid was significantly decreased (P<0.05), the survival rate and proliferation ability of the cells were significantly decreased (P<0.05), the expression levels of G6PD mRNA and protein were decreased (P<0.05)；compared with empty vector plasmid transfection group, the level of glucose in the culture medium in SIRT2 plasmid transfection group was decreased significantly (P<0.05), the level of lactic acid was significantly increased (P<0.05), the proliferation ability and clone formation rate of the cells were significantly increased (P<0.05).The expression levels of SIRT2 and G6PD proteins in colorectal cancer tissue were significantly higher than that in paracancerous tissue (P<0.05). Compared with SIRT2-siRNA#3 group, the level of glucose in the culture medium in SIRT2-siRNA+G6PD plasmid co-transfection group was significantly decreased (P<0.05), the level of lactic acid in the culture medium was significantly increased (P<0.05), and the proliferation ability and clone formation rate of the cells were significantly increased (P<0.05). Conclusion SIRT2 promotes the aerobic glycolysis and growth and proliferation of the colorectal cancer cells, and its mechanism may be related to the expression of G6PD regulated by SIRT2. [ABSTRACT FROM AUTHOR]

Immobilized enzymes were prepared by using strongly alkaline macroporous resin, amino resin, and epoxy resin as immobilized carriers of glucose oxidase and catalase, respectively, and their effect on low-pH gluconic acid production was assessed. The results of the acid resistance test showed that all the three immobilized enzymes had stronger acid resistance than did the free enzyme. The free enzyme and the three immobilized enzymes were used to prepare gluconic acid under low pH conditions (pH value 3.5). The reaction times required for the strongly alkaline macroporous resin-immobilized enzyme, amino resin-immobilized enzyme, and epoxy resin-immobilized enzyme were 40, 24, and 27 h respectively. The enzymatic activity loss rates were 46.44%, 3.42%, and 21.84%, respectively. The free enzyme was completely inactivated during the reaction. The clarity and chromaticity of the three immobilized enzyme reaction solutions were significantly better than those of the free enzyme reaction solutions. The preparation process of amino resin-immobilized enzyme-after further optimization through orthogonal experiments - was as follows: the concentration of mixed enzyme solution was 10%, the ratio of glucose oxidase to catalase was 1.5:1, and the fixation temperature was 25 °C. Under these conditions, the recovery rate of amino resin-immobilized enzyme was as high as 93.15%, and the conversion time of the 100 g/L glucose solution was 23 h. At the end of the reaction, the ratio of gluconic acid to sodium gluconate had reached 0.899:1 (m/m), and the amino resin-immobilized enzyme was stable. Based on these results, we conclude that the amino resin immobilized enzyme can be used to establish a low-pH immobilized enzyme gluconic acid production process, with the goal of producing high-quality gluconic acid products. [ABSTRACT FROM AUTHOR]

To investigate the effects of glucose oxidase (GOD) induced oxidation on the gel properties of silver carp surimi, the changes of elastic modulus (G'), loss modulus (G''), gel strength, whiteness, microstructure, water holding capacity, moisture distribution and protein crosslinking of surimi added with different GOD contents (0, 0.1‰, 0.3‰, 0.5‰, 0.7‰, 0.9‰) were analyzed. The results showed that G', G'', gel strength, whiteness and water holding capacity increased firstly and then decreased with the increase of GOD addition, and all of them reached the maximum when the addition was 0.5‰. When GOD addition was in the range of 0.1‰~0.5‰, the free water and bound water content of gelatin decreased, the content of immobile water increased, and the gel network of surimi was denser with the increase of GOD addition. While adding excessive GOD (0.7‰~0.9‰), the pore size of surimi gel increased and gel network was destroyed. As indicated by SDS-PAGE patterns, appropriate addition of GOD could promote the formation of disulfide bonds and non-disulfide covalent bonds between protein molecules, thus the gel properties of silver carp surimi were improved.

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Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The experiment was to study the effect of different levels of glucose oxidase (GOD) on growth performance, immune function, serum biochemical indexes and intestinal morphological structure of broilers. A total of 600 healthy one-day-old AA broilers were randomly divided into three groups, with five replicates in each group and 40 in each replicate (half in male and female). The control group was basic diet, and the experimental group Ⅰ and group Ⅱ were supplement with 400 and 1 000 U/kg GOD4000 in basis diet, respectively. The experiment lasted for 42 d. The results showed that compared with the control group, group Ⅰ and test group Ⅱ can significantly increased the average body weight, average daily gain and average daily feed intake of broilers at each stage (P<0.05), and reduced feed to gain ratio (P>0.05). GOD4000 had no significant effect on the immune organ index of broilers (P>0.05). Compared with control group, the serum urea nitrogen content of broilers in group Ⅰ was significantly decreased at the age of 43 d (P<0.05). Compared with control group, the serum total protein content of broilers at 43 d of age in group Ⅱ was significantly increased (P<0.05), and the serum urea nitrogen and total cholesterol levels were significantly decreased (P<0.05). Compared with control group, the jejunal villus height of broilers at 22 and 43 d in group Ⅱ was significantly increased (P<0.05), and the ratio of jejunal villus height to crypt depth at 43 d was significantly increased (P<0.05). The study indicates that GOD4000 can improve the growth performance of broilers, and the effect is enhanced with the increase of the addition level. The addition of 1 000 U/kg GOD4000 to diet can further improve the protein metabolism of broilers and improve the structure of jejunum villi. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Environmental Science & Technology (10036504) is the property of Editorial Board of Environmental Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The experiment was to investigate the effect of different enzyme preparations and their combinations on growth performance and immune performance of growing minks. A total of eighty 75-day-old Jinzhou black male minks were randomly divided into four groups, with 20 replicates in each group and one mink in each replicate. Minks in experimental group Ⅰ (control group) were fed with a basal diet. The basal diet was supplemented with 200 g/t glucose oxidase in experimental group Ⅱ and 200 g/t catalase in experimental group Ⅲ. Experimental group Ⅳ was fed with a basal diet supplemented with 200 g/t glucose oxidase +200 g/t catalase. The experiment lasted for 60 d. The results showed that compared with control group, the average daily gain of minks in test group was significantly increased (P<0.05), the feed to gain ratio in test group Ⅳ was significantly decreased (P<0.05). Compared with control group, diarrhea rate and mortality rate of minks in test group were significantly decreased (P<0.05). The activities in serum of minks of T-SOD and GSH-Px were significantly increased (P<0.05). Serum IgG, IL-2 and IFN- γ content in test group Ⅳ were significantly increased (P<0.05). The experiment indicates that the enzyme preparation can greatly improve the growth performance, antioxidant capacity and immune performance of minks during growing period, and reduce the diarrhea rate and mortality rate. The mixed addition of glucose oxidase and catalase had the best effect on growth and development of minks during the growing period. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The interface modification of electrochemical sensors is an important way to improve their detection performance. Among them, enhancing the biocompatibility and electrical conductivity of the electrochemical sensing interface is a major challenge for the development of electrochemical sensors. In this work, the surface of glassy carbon electrode is functionalized based on WS2@Au quantum dots （WS2@Au QDs）prepared by in situ one-step reduction, which is used to immobilize oxidoreductase, thus realizing the construction of high-performance biosensors. By virtue of the excellent biocompatibility and electrical conductivity of WS2@Au QDs, the WS2@Au QDs can effectively protect the catalytic activity of GOx and promote the direct electron transfer between GOx and the electrode, and the electron transfer rate constant reaches 2. 25 s −1. Based on the good electrocatalytic effect of the sensor on glucose, the method has been successfully applied to the detection of glucose, with a linear range of 5~50 μmol/L and a detection limit of 1. 5 μmol/L, and the sensor shows good selectivity, reproducibility and stability. These results suggest that the WS2@Au QDs has potential important application prospects in the interface modification of biosensors, which lays a good research foundation for the construction of high-performance biosensors. [ABSTRACT FROM AUTHOR]

The number of active groups on the molecular chain of chitosan is limited. Tn order to further increase the amount of chitosan enzyme and the biocatalysis stability of immobilized enzyme, in this study, 3-aminopropyl triethoxysilane (A PTES) and gl utaric anhydride (GA) are used as surface modifiers to prepare carboxyl functionalized porous magnetic chitosan microspheres (PMCSM-COOH), and Glucose oxidase (GOT)) and Catalase (CAT) are successfully immobilized. The results show that GOT) relative enzyme activity reaches the highest value of (1 31.91 ±0.58) % when the mass ratio of PMCSM-C00H/GOT)/CAT is 100/9.34/1 0.7& Compared with the free enzyme, the temperature stability, acid-base stability and storage stability of the co-immobilized enzyme are improved, and the affinity with the substrate is also enhanced. After repeated use for 1 0 times, the co-immobilized enzyme still retains a high relative enzyme activity of (79.1 6 ± 1.98) %• The secondary structure of free enzyme and immobilized enzyme show that the relative a -helix content of immobilized enzyme is higher than that of free GOD, so that immobilized enzyme has better structural rigidity and stability than free enzyme. [ABSTRACT FROM AUTHOR]

The experiment was to study the effect of glucose oxidase（GOD） and lactic acid bacteria on intestinal morphology and microbial flora of broilers. 1 200 one-day-old AA broilers were randomly divided into four groups.Group Ⅰ was control group drinking normal water. Group Ⅱ was GOD group, adding 0.1% GOD to drinking water.Group Ⅲ was lactic acid bacteria preparation group, adding 1.5×105 CFU/（g·d） lactic acid bacteria preparation to drinking water of each chicken. Group Ⅳ was combined application group of GOD and lactic acid bacteria preparation,and the added dose was the same as that of group Ⅱ and group Ⅲ. The test period was 42 d. The results showed that on the 21 st day of the test, compared with control group, the height of duodenal villi of broilers in each test group was significantly increased（P<0.05）, and V/C was significantly increased（P<0.05）. The height of jejunum villi of broilers in each experimental group was significantly increased（P<0.05）. The V/C of jejunum of broilers in the experimental group Ⅳwas significantly higher than that of control group（P<0.05）. There was no significant difference in crypt depth between each group（P>0.05）. On the 42 nd day of the test, compared with the control group, the height of the duodenal villi of the group Ⅱ and group Ⅳ was significantly increased（P<0.05）, and the V/C of the test group Ⅳ was significantly increased（P<0.05）. Compared with control group, the Shannon index of each experimental group was higher than that of control group（P>0.05）, and the Simpson index was lower than that of control group（P>0.05）. Compared with control group, the content of Bacteroidetes in group Ⅱ was extremely increased（P<0.01）, and the content of Bacteroidetes in group Ⅳ was significantly increased（P<0.05）, and the content of Bacteroidetes was significantly decreased（P<0.05）. The ratio of Parabacteroides and Faecalibacterium in the group Ⅱ was significantly higher than that of the other three groups（P<0.05）. The study indicates that the combination of GOD and lactic acid bacteria in drinking water can improve the intestinal morphology, microbial flora structure of broilers. [ABSTRACT FROM AUTHOR]

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The experiment was to study the effect of the combination of glucose oxidase (GOD) and Lactobacillus on growth performance and immune function of broilers. A total of 1200 one-day-old AA broilers were randomly divided into four groups: group Ⅰ (control group), group Ⅱ, group Ⅲ and group Ⅳ. The control group was free to drink water, group Ⅱ was added 0.1% GOD in the drinking water of each chicken daily, and group Ⅲ was added 1.5×105 CFU/g Lactobacillus preparation in the drinking water of each chicken daily. In test group Ⅳ, 0.1% GOD and 1.5×105 CFU/g Lactobacillus were added to each chicken's drinking water daily. The test period was 42 d. The results showed that compared with group Ⅰ, the average daily feed intake (ADFI) of broilers in group Ⅱ, group Ⅲ and group Ⅳ had no significant difference ( P >0.05), the average daily gain (ADG) of group Ⅳ within 21 d was significantly increased ( P <0.05), the feed to gain ratio (F/G) was significantly reduced ( P <0.05). The thymus index, bursa index and spleen index of broilers in group Ⅳ increased significantly on 21 d ( P <0.05). On 42 d, the bursa index increased significantly ( P <0.05). Compared with group Ⅰ, the titers of Newcastle disease antibody in broilers of group Ⅳ were significantly increased on the 21 d and 28 d ( P <0.05), the titer of avian influenza H9 antibody on 21, 28, 35 and 42 d was significantly increased ( P <0.05), 21 d and 42 d serum immunoglobulin G (IgG) and 21 d immunoglobulin A (IgA) levels significantly increased ( P <0.05). The experiment indicates that the combined application of GOD and Lactobacillus can improve the growth performance and immune function of broilers, and the effect is significantly better than that of single application. [ABSTRACT FROM AUTHOR]

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Research progress of three-dimensional conductive polymer biosensor was discussed. Principle of conductive polymer biosensing and definition of three-dimensional structure conductive polymer were briefly introduced. Three-dimensional conductive polymer with different materials and spatial structures application in enzyme biosensor, DNA biosensor, glucose biosensor and microbial sensor was emphatically summarized. It is considered that due to better electrical conductivity and special three-dimensional spatial structure of three - dimensional conductive polymer, it can be used as biosensor modify electrical materials, which can greatly improve sensitivity and detection limit of biosensors and can be widely used in biosensor field. [ABSTRACT FROM AUTHOR]

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Glucose oxidase (GOD) is a kind of non-toxic aerobic dehydrogenase with high specificity and high efficiency. GOD has the effect of inhibiting the growth and reproduction of harmful microorganisms, improving animal intestinal health and animal production performance. The paper reviews the biological functions of glucose oxidase and its effect on animal production performance, to provide reference for application of glucose oxidase in animal production. [ABSTRACT FROM AUTHOR]

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Steamed bread is a traditional fermented pasta product in China, which plays an important role in the dietary structure. With the improvement of people's living standards and the acceleration of life pace in recent years, steamed bread has become a popular fast food product for consumer. However, the shelf life of steamed bread is short and easy to be aged, which greatly affects the industrialized production of steamed bread. Frozen dough technology can provide an economic and effective technical way. However, the relevant research reports pointed out that the water in the dough formed ice crystals in the process of frozen storage. Because of the temperature fluctuation, recrystallization will occur, which will destroy the network structure of the dough, make the dough appear cracks after freezing, reduce the gas holding capacity, as well as affect the quality of frozen dough and its cooked products. At the same time, ice crystal will also lead to the damage and rupture of yeast cells, such as the damage of its respiratory system, the reduction of yeast vitality and the lack of gas production, which will cause the decline of dough specific volume, texture and sensory quality. The use of dough quality improver can enhance the structure of gluten network, protect yeast activity, improve the quality of dough, and prolong the storage period of frozen dough. To study the effect of different improvers on the quality of frozen dough and its cooked products, and to select the compound improvers which can improve the quality of the products better, it has a certain practical significance in the actual production. In order to improve the quality of frozen dough and to clarify the effect of different food improvers on the quality of unwrapped frozen dough, this paper studied compound thickeners (carrageenan, guar gum, sodium alginate) and compound emulsifier (diacetyl tartrate monoglyceride, carboxymethyl cellulose), complex enzyme preparations (glutamine transaminase, glucose oxidase) and trehalose on the quality of unwrapped frozen dough steamed bread, through single factor and response surface experimental design, the effect of different compound quality improver on the specific volume, texture and sensory quality of frozen dough. Studies have shown that the effect of trehalose and enzyme preparation on the specific volume of frozen dough is extremely significant (P<0.01). Under the compounding process of adding 0.4 % emulsifier, 0.8 % thickener, 45 mg/kg enzyme preparation and 2 % trehalose, the specific volume of the frozen dough steamed bread reached the level of 3.15 mL/g, and its hardness and the springiness also reached a superior value of 926.832 g and 0.912 8, respectively. At the same time, the frozen dough which was not added and added with the compound quality improver for 0-2.5 month was observed by scanning electron microscopy. It was found that most of the small starch granules in the frozen dough without the addition of the modifier were exposed outside, the particles were distinct, and the internal structure was uneven. The frozen dough starch granules with the compound quality improver added in the gluten network, the protein network structure is clear and complete, the internal structure of the frozen dough is even and tight. [ABSTRACT FROM AUTHOR]

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Objective: To establish Human glomerular endothelial cells (Human glomerular endothelial cells, HRGEC) model of insulin resistance by the different concentrations of palmitic acid and glucose in vitro induced. Methods: Human glomerular endothelial cells as the research object, the different concentration of palmitic acid (100, 200, 300, 400, 500 μmol/L) and different concentrations of glucose (20, 30, 40, 50, 60 mmol/L) for 24 hours and 48 hours respectively, to detect palmitic acid and glucose effects on glucose consumption and HRGEC survival determined by MTT method and glucose oxidase method, to detect P-IRS, the IRS, AKT and P-AKT (Ser473) by protein immunoblot method. Results: 1. When palmitate was given 500 μmol/L for 24 h, cell activity significantly decreased compared with the normal group(P<0.01), and cell survival rate significantly decreased when palmitate concentration was more than or equal to 300 μmol/L for 48 h (P<0.01). Compared with the control group, palmitate intervention at 300 μmol/L, 400 μmol/L and 500 μmol/L for 24 hours could significantly reduce the glucose consumption of cells (P<0.05). Intervention at 200 μmol/L, 300 μmol/L, 400 μmol/L and 500 μmol/L for 48 hours significantly reduced glucose consumption (P<0.01). Compared with the blank group, glucose intervention of 40mmol/L, 50 mmol/L and 60 mmol/L for 24 hours could reduce glucose consumption of human glomerular endothelial cells (P<0.05). Compared with the blank group, the glucose consumption of human glomerular endothelial cells was significantly reduced by the intervention of 30mmol/L, 40 mmol/L, 50 mmol/L and 60mmol/L for 48 hours(P<0.01). 3. After 24 h of glucose stimulation of human glomerular endothelial cells (HGREC) at different concentrations, the results showed that the levels of p-IRS /IRS and p-AKT/AKT (Ser473) decreased with glucose stimulation of 50 mmol/L and 60 mmol/L for 24 hours (P<0.01), while no significant changes were observed in other groups (P>0.05). Conclusion: The high glucose induction method could establish the insulin resistance model of HRGEC cells, which has the advantages of short modeling period, easy repetition and strong controllability, and could be used for the study of the mechanism of insulin resistance in diabetes mellitus and the screening of traditional Chinese medicine components. [ABSTRACT FROM AUTHOR]

： Through adjusting the ratio of plant fibers, beating the pulp to increase the fiber surface roughness ? adding lignin, grafting hydroxyl ? carboxyl groups and other micro-regula¬tion means?the adsorption performance of plant fibers to glucose oxidase was enhanced. SEM results showed that glucose oxidase was mostly loaded on the surface of plant fibers，which provided a basis for microregulation on the surface of fibers. In order to further explore the adsorption of enzyme and fiber, glucose oxidase was labeled with fluorescence ? and character¬ized by laser confocal fluorescence microscope and fluorescence spectrometer. The results showed that the enzyme uptake of various plant fibers increased by 95% to 220% after microregulation ? which means microregulation is effective and has broad spectrum. After micro¬control, the core physical properties of paper based on paper chip were measured. The results showed that the increase in tensile strength of paper based on paper chip after micro-control was 4. 9 % to 39 %，and the increase in tensile strength when the beating degree was increased was 520% to 740%. After microcontrol,the height of capillary water absorption of paper chip paper base increased by 0. 1 % 〜13. 9 %. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)