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ObjectiveTo explore the mediating effect of depressive symptoms on occupational stress and work-related musculoskeletal disorders (WMSDs) in employees of petrochemical enterprises for developing intervention measures on WMSDs. MethodsTotally 4 066 employees were recruited with cluster sampling from a petrochemical enterprise in Jiangsu province for an online survey conducted during July – October 2021. Core Occupational Stress Scale (COSS), Patient Health Questionnaire-9 (PHQ-9)，and two questionnaire on demographics and WMSDs developed by Chinese researchers previously were used in the survey. Spearman rank correlation analysis was adopted to analyze the relationships between occupational stress, depression symptoms, and WMSDs; hierarchical regression analysis and bootstrap method were used to verify the mediating effect of depressive symptoms on the correlation between occupational stress and WMSDs. ResultsOf the 3 763 participants with valid responses, 2 934 (77.97%) were assessed as having WMSDs during past one year. The participants′ mean scores were 3.47 ± 3.17 on WMSDs and 10.04 ± 5.99 on depressive symptoms; the mean score on occupational stress was 45.79 ± 9.48, with the dimensional scores of 19.81 ± 3.75 on social support, 15.85 ± 4.94 on organization and reward, 12.35 ± 3.39 on demand and effort, and 4.62 ± 1.80 on autonomy, respectively. Spearman rank correlation analysis revealed following significant correlations among partici-pants′ scores on WMSDs, occupational stress and its dimensions, and depression symptoms: (1) positive correlations of WMSDs with overall occupational stress (r = 0.334), occupational stress dimensions of organization and reward (r = 0.284) and demand and effort (r = 0.202), and depression symptoms (r = 0.463) (P < 0.01 for all); (2) negative correlations of WMSDs with occupational stress dimensions of social support (r = – 0.256) and autonomy (r = – 0.141) (both P < 0.01); (3) positive correlations of depression symptoms with overall occupational stress (r = 0.621), occupational stress dimensions of organization and reward (r = 0.540) and demand and effort (r = 0.410) (P < 0.01 for all); (4) negative correlations of depression symptoms with social support (r = – 0.419) and autonomy (r = – 0.196) (both P < 0.01); (5) positive correlations of overall occupational stress with occupational stress dimensions of organization and reward (r = 0.848) and demand and effort (r = 0.639) (both P < 0.01); and (6) negative correlations of overall occupational stress with occupational stress dimensions of social support (r = – 0.648) and autonomy (r = – 0.310) (both P < 0.01). The results of mediating effect analysis showed that depressive symptoms had a partial mediating effect on the correlations of WMSDs with occupational stress dimensions of social support (effect value = – 0.137; confidence interval [CI]: –0.155, – 0.120; effect percentage = 72.87%) and demand and effort (effect value = 0.177; CI: 0.159, 0.196; effect percentage = 78.67%) but a complete mediating effect on the correlations of WMSDs with occupational stress dimensions of organization and reward (effect value = 0.146; CI: 0.131, 0.160; effect percentage = 90.12%) and autonomy (effect value = – 0.126; CI: – 0.158, – 0.094; effect percentage = 98.44%). ConclusionAmong the employees in a petrochemical enterprise in a province of China, the detection rate of WMSDs was high and depressive symptoms showed a mediating effect on the correlation of occupational stress with WMSDs, suggesting that the prevalence of WMSDs could be decreased through intervention on depression symptoms in the occupational population.

BackgroundCumulative fatigue without intervention will seriously threaten the physical and mental health of workers. Shift work and life satisfaction are strongly associated with fatigue accumulation. ObjectiveTo explore the effects of life satisfaction, shift work, and their interaction on cumulative fatigue in petrochemical employees, and to provide a scientific basis for preventing cumulative fatigue. MethodsAll staff of a petrochemical enterprise were selected by cluster sampling for a cross-sectional study from July to October 2021 in Jiangsu Province. A questionnaire designed by the project team was used to collect information on shift work; and life satisfaction and cumulative fatigue were investigated by the World Health Organization Five-item Well-Being Index and the Self-diagnosis Checklist for Assessment of Worker’s Fatigue Accumulation respectively. A logistic regression model was used to analyze the influences of life satisfaction and shift work on cumulative fatigue. Multiplicative and additive models were applied to analyze the interaction effect of life satisfaction and shift work. ResultsA total of 4066 questionnaires were returned, of which 3763 were valid, with an effective recovery rate of 92.5%. The percentage of cumulative fatigue in the petrochemical employees was 63.2% (2377/3763), and the percentages of low life satisfaction and shift work in the petrochemical employees were 53.6% (2016/3763) and 54.2% (2041/3763), respectively. The results of univariate analysis showed no significant difference in cumulative fatigue among different marital status groups (P=0.176), and there were statistically significant differences in cumulative fatigue among the petrochemical employees in different groups of age, gender, educational level, average monthly income, job title, length of service, working hours, night shift, smoking, drinking, physical exercise, life satisfaction, and shift work (P

BackgroundN6-methyladenosine (m6A) RNA methylation may play an important role in the process of malignant transformation of cells induced by environmental carcinogens. However, the specific roles and mechanisms need to be further explored.ObjectiveTo explore the role and mechanism of m6A binding protein insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) in the malignant transformation of human gastric mucosal epithelial cells GES-1 induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).MethodsBased on the GES-1 malignant transformation cells MC-30, a stable knockdown IGF2BP3 MC-30 cell line (MC30-shIGF2BP3, abbreviated as MC30-shI3) was constructed by lentiviral transfection technology, and a negative control group (MC30-NC) was also prepared. Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting were applied to detect the mRNA expression and protein levels of IGF2BP3. RNA binding protein immunoprecipitation (RIP-qPCR) was used to examine the combination between IGF2BP3 protein and MYC mRNA in malignant cells MC-30. Furthermore, the stability of MYC mRNA was detected by actinomycin D assay. CCK-8 and Transwell respectively were employed to detect cell proliferation, migration, and invasion. Western blotting was applied to detect the expression of EMT markers (N-cadherin, Vimentin, α-SMA, and Snail). The role of the downstream target gene MYC was further elucidated by a rescue assay in MC30-shI3 cells transfected with a plasmid overexpressing MYC to observe changes in cellular phenotypes (proliferation, migration, invasion) and expression of key EMT proteins.ResultsCompared with the control group, the expression of IGF2BP3 mRNA was up-regulated after 5, 10, 20, and 40 μmol·L−1 MNNG infection of GES-1 cells (P