1. [Influence of exogenous RNA upon expression of mouse albumin gene and its sensitivity to DNase I].
- Author
-
Zhang HY, Liu AJ, Liu FY, Wang XF, and Lü ZJ
- Subjects
- Albumins genetics, Animals, Deoxyribonuclease I metabolism, Dose-Response Relationship, Drug, Fibroblasts drug effects, Fibroblasts physiology, Fishes, Genes drug effects, Kidney chemistry, Liver chemistry, Lung chemistry, Male, Mice, Prostate cytology, Prostate drug effects, RNA chemistry, Rabbits, Rats, Albumins biosynthesis, Gene Expression drug effects, RNA pharmacology
- Abstract
Cultured mouse fibroblasts were treated with rabbit liver RNA. Rat liver RNA was injected into mouse prostates. Influence of exogenous RNA upon expression of mouse albumin gene was detected by immunohistochemical staining. Nucleuses of cultured mouse fibroblasts treated with different RNAs were isolated and digested with DNase I. Mouse albumin gene was amplified by PCR to detect levels of its digestion. It was found that exogenous RNAs could increase the sensitivity of mouse albumin gene to DNase I digestion and promote its expression.
- Published
- 2002