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Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to determine an easy leaf age(LA)diagnostic method of panicle differentiation stage of sorghum, the panicle differentiation of sorghum and the relationship between differentiation stage and LA and leaf age index(LAI)were investigated. Hybrid cultivar ‘Jinyunuo 3' and conventional cultivar ‘Guojiaohong 1' were used as materials planted in three sowing dates, the panicle differentiation stage and corresponding LA were recorded. The results were as follows:(1)The panicle differentiation could be divided into five stages, i. e. vegetative stage, elongation stage, panicle branch differentiation stage, spikelet and floret differentiation stage, gynoecium and stamen formation stage. The sterile spikelet with stalk could develop to gynoecium and stamen formation stage.(2)With the detention of sowing date, the starting date of panicle differentiation was advanced, and the growth duration shorted, and LA and LAI of each stage of panicle differentiation were decreased, but there were differences among varieties.(3)The relationship between the panicle differentiation stage and LAI conformed to linear regression correlation, with a formula of panicle differentiation stage(Yi)to LAI(Xi) of Yi=bXi+a.(4)With the detention of sowing date, the parameter b and |a| in formula of ‘Jinyunuo 3' were increased, while those of ‘Guojiaohong 1' were decreased. To sum up, it is viable to infer panicle differentiation stage applying LAI, and the results provide theoretical basis for sorghum cultivation index management. [ABSTRACT FROM AUTHOR]

Copyright of Bulletin of Botanical Research is the property of Bulletin of Botanical Research Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to uncover the molecular mechanisms of FRUITFULL(FUL)homologous genes involving in regulating flower and fruit development in buckwheat, an 837 bp of FeFUL2 cDNA containing a 690 bp full ORF(Open Reading Frame)encoding 229 amino acids(GenBank Accession Number MG779493.1)was isolated from a Fagopyrum esculentum mutant line with long pistil and long stamen (lpls) through homologous cloning. Moreover, the FeFUL2 cDNA contains a 30 bp 5'UTR(untranslated region, UTR)and a 117 bp 3'UTR including poly-A. The results showed that the buckwheat FeFUL2 was classified into the core eudicot euFUL lineages of AP1/FUL subfamily MADS-box transcription factors through phylogenetic, protein alignment and sequence analyses. In addition, FeFUL2 was classified transcription contained a highly conservation MADS-box domain(1-57)with 57 amino acids(aa), a secondary conserved K domain(91-159)with 69 aa, as well as two conserved motifs: FUL motif and paleo AP1 motif lying variable C terminal region. The highly conserved MADS domain was responsible for DNA binding, dimerization and nuclear localization of MADS-domain transcription factors. The secondary conserved K domain was involved in the formation of amphipathic helices and responsible for protein dimerization and multimeric complex formation protein-protein interactions. Finally, the C domain was important for transcriptional activation and multimeric complex formation. Moreover, the C terminal region of FeFUL2 was variable in sequence and length comparing with other euFUL-like transcriptors, which suggested that FeFUL2 may play different roles regulating flower and fruit development with FUL-like homologs from other species. qPCR revealed that FeFUL2 expression was detectable in all tissues including root, stem, leaf, tepal, stamen, gynoecium and 4-day-old juvenile fruit. However, FeFUL2 expression level in tepal was significantly higher than those in other organs(LSD, P<0.01). In addition, FeFUL2 expression level in stamen, gynoecium and 4-day-old juvenile fruit displayed no significant differences(LSD,P>0.05), but FeFUL2 expression level in stem and leaf was significantly higher than root(LSD,P<0.05). However, FeFUL2 expression level in stem and leaf showed no significant differences(LSD,P>0.05). Above all, our data suggest that the function of FeFUL2 may show a difference with other euFUL-like gene, and FeFUL2 play a major role involving in perianth development. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)