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Objective: To investigate the effects of Fn14 on regulating cisplatin (DDP) sensitivity in human ovarian cancer cells. Methods: Fn14 protein expression levels in SKOV3, CAOV3, OVCAR3, ES2, 3AO, A2780 and A2780/DDP were detected through Western blotting. The expression of Fn14, caspase-3, cleaved caspase-3 and Bcl-2 were accessed by Western blotting in lowest expression of Fn14 cell line when transfected with Fn14 plasmid. The half cell lethal dose (IC50) of DDP and the ability of proliferation in A2780/DDP cells were assayed by CCK-8 kit. The ratio of apoptosis in A2780/DDP cells were determined by flow cytometry. Results: A2780/DDP cells exhibited the lowest expression of Fn14 in these cell lines. Fn14 transfer had no effect on the proliferation of A2780/DDP cells, whereas it could reduce the IC50 of DDP in A2780/DDP cells (P＜0.05). When Fn14 was transferred, the apoptosis induced by DDP was increased in A2780/DDP cells, meanwhile, the expression of apoptosiscorrelated protein caspase-3 was up-regulated and anti-apoptotic protein Bcl-2 was down-regulated (P＜0.05). Conclusions: Fn14 may increase chemosensitivity to DDP by apoptosis pathway in human epithelial ovarian cancer cells. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression of tumor necrosis factor-like weak inducer of apoptosis (TWEAK)/ fibroblast growth factorinducible 14 (Fn14) in the serum and colon tissue of Crohn's disease (CD) and to elucidate whether the expression of TWEAK/Fn14 can be used as an indicator for intestinal fibrosis., Methods: Blood samples from 67 CD patients and 33 healthy controls were collected to measure the level of TWEAK by ELISA. Meanwhile,colon samples from 29 CD patients received colectomy and the normal colon tissues from 15 patients with colon cancer were included. The expression of TWEAK and Fn14 in colon samples was analyzed by immunohistochemistry (IHC). The number of the cells with Fn14,αsmooth muscle actin (αSMA) double positive was measured by immunofluorescent double staining., Results: The level of serum TWEAK in CD patients was higher than that in healthy controls ( P <0.01),which was positive correlated with colectomy ( r =0.295, P =0.015),intestinal stenosis ( r =0.290, P =0.017) and intestinal obstruction ( r =0.453, P= 0.000 1). ROC analysis displayed the area under the curve of serum TWEAK for predicting intestinal stenosis in CD patients was 0.67 (95% CI 0.540.80, P =0.020). IHC results showed that the expression of TWEAK and Fn14 were increased in colon samples from CD patients with intestinal obstruction ( P <0.05). The number of Fn14 and αSMA positive cells was significantly increased in CD patients with intestinal obstruction ( P <0.05)., Conclusion: TWEAK/Fn14 pathway may play an important role in CD related intestinal fibrosis.

Objectives: To investigate the effect of tumor necrosis factor -related weak inducer of apoptosis (TWEAK)/fibroblast growth factor-inducible 14 (Fn14) on the proliferation and growth of pancreatic cancer., Methods: Human pancreatic cancer (50 cases), chronic pancreatitis (40 cases) and normal pancreatic tissues (30 cases) were collected in West China Hospital from January 2012 to December 2013. TWEAK and Fn14 expressions in these tissues were checked with hematoxylin-eosin (HE) staining and immunohistochemistry method. Relationship between TWEAK expression and clinicopathological features of pancreatic cancer was analyzed., Results: TWEAK expression rate was 36% (18/50) in pancreatic cancer, higher than that in chronic pancreatitis (17.5%, 7/40) and normal pancreatic tissues (13.3%, 4/30) ( P <0.05) .Expression intensity of TWEAK in three groups was also obviously ( P <0.05). The expression rate of Fn14 was 4.0% in pancreatic cancer , 7.0% in normal pancreatic tissues , and 0% in chronic pancreatitis. TWEAK positive expression rate and high expression rate in pancreatic cancer were higher in IIB group ( P <0.05). Pathological grade was not related to TWEAK expression., Conclusions: TWEAK/Fn14 involved in the progression of pancreatic cancer. In the tissue of pancreatic cancer, TWEAK was highly expressed, and Fn14 was lowly expressed.