1. Molecular markers of esp: A recessive epistatic suppressor gene of recessive genic male sterile Brassica napus L.
- Author
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LI Jing-jing, XIE Yan-zhou, HONG Deng-feng, LIU Ping-wu, HE Qing-biao, and YANG Guang-sheng
- Subjects
RUTABAGA ,EPISTASIS (Genetics) ,STERILITY in plants ,GENETIC polymorphisms ,PLANT genetics - Abstract
9012AB is a recessive epistatic genic male sterility (REGMS) two - type line of Brassica napus L. The sterility is controlled by two recessive duplicate genes ms
3 ms3 ms4 ms4 and one recessive epistatic inhibitor gene espesp. To effectively identify REGMS lines and their temporary maintainer lines, five AFLP (amplified fragment length polymorphism) markers (L1, L2, L3, L4, L5) in couple to esp and two AFLP markers (L6, L7) in repulsion to esp were identified through screening 1 024 primer combinations using 9012B and T45 segregation population. All the seven markers were located on one the same side of esp gene. Markers L6 and L7 co - segregated with Esp gene. Markers L1, L6 and L7 were successfully converted into SCAR (sequence characterized amplified region) markers as SCL1, SCL2 and WSCL3, respectively. The accuracy rates of SCL1, SCL2 and WSCL3 were 90% , 100% and 100% , respectively. Result showed their potential in marker assisted selection of 9012AB. [ABSTRACT FROM AUTHOR]- Published
- 2011