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Objective: To investigate the influence of genetic susceptibility genes for myeloid tumors on the clinical characteristics of patients with myeloproliferative neoplasm (MPN)., Methods: Two hundred and thirty-two patients with MPN diagnosed at the Second Hospital of Tianjin Medical University from September 2017 to December 2021 were collected, myeloid neoplasm-related genes were detected by targeted next-generation sequencing, and germline mutations were verified. The clinical characteristics and prognosis of MPN patients with germlines mutations in the genetic susceptibility gene for myeloid neoplasm were analyzed., Results: The germline mutation carrier rate of myeloid neoplasm genetic susceptibility gene in MPN patients was 21.6% (50/232), and the PV, ET and PMF patients carrying germline mutations of genetic susceptibility gene for myeloid neoplasm were 25/114 (21.9%), 8/69 (11.6%) and 17/49 (34.7%), respectively, among which PMF patients had the highest carrier rate ( P =0.01) and were older ( P =0.02). The incidence of chromosomal abnormalities in MPN patients carrying the genetic susceptibitity genes for myeloid neoplasm was higher than that in the non-carrier group (26.5% vs 11.8%, P =0.05). Germline mutations in the genetic susceptibility gene of myeloid neoplasm in MPN patients were mainly concentrated in the RAS pathway, and patients with germline mutations in the genetic susceptibility gene of myeloid neoplasm associated with the RAS pathway had shorter survival without AML progression ( P <0.0001). The overall survival time in MPN patients with CBL and TP53 germline mutations was shorter than that of non-carrier group ( P =0.001; P =0.043)., Conclusion: In MPN, PMF patients are more likely to carry germline mutations in the genetic susceptibility gene for myeloid neoplasm. MPN patients with germline mutations carring the genetic susceptibility gene for myeloid neoplasm are prone to chromosomal abnormalities; Patients with MPN who have germline mutations in the genetic susceptibility gene for myeloid neoplasm in the RAS pathway are more likely to develop AML; CBL and TP53 germline mutations affect the overall survival of MPN patients.

We took Xuchang section of Qingyihe River Basin as study object to explore biodiversity in Qingyihe River. On the basis of field survey and in-laboratory analysis, community characteristics of aquatic macrophytes, riparian zone plants and macrobenthos, with environmental characteristics of water and surface sediment were investigated. Results showed that a total of 12 species of aquatic macrophytes, 66 species of riparian zone plants, and 10 species of macrobenthos were found. The communities varied sharply with the changes in the environmental factors, such as NH 4 + -N, TN, TP in the water and oxidation-reduction potential (ORP), TP, Cd, Hg, Zn in the surface sediment. The CCA ordination results indicated that COD, temperature, pH, DO, TP in the water were key factors influencing aquatic macrophytes, while ORP, pH in the water and Ni, Hg, TOC, Pb, Cd in the surface sediment were key factors for riparian zone plants. pH, temperature, ORP, As, Pb, Ni in the surface sediment and temperature in the water were the key factors influencing community characteristic of macrobenthos. In Qingyihe River basin of Xuchang section, the water had been polluted and the biodiversity of communities was low, so it is urgent to treat wastewater along the river as well as protect and recover communities.

Microbial contamination in karst groundwater continually increases and tracing the source researches has become a hot topic for international researchers. In this study, Laolongdong underground river at Nanshan, Chongqing was chosen as an example to adopt filter membrane methods to monitor the fecal microbial contaminations including the total bacterial concentration (TB), the total E. coli concentration (TE), the total fecal coliform (FC) and the total fecal Streptocoocci (FS). Bacteriodes was used as an indicator and PCR-DGGE analysis was used to trace fecal contamination sources in karst groundwater. The results suggested that groundwater in this area was seriously polluted by microbes from feces. The concentrations of microbial parameters exceeded limited levels greatly and the total bacterial amounts ranged 10-2.9 x 10⁷ CFU · mL⁻¹, the concentrations of E. coli were between 4.3-4.0 x 10⁵ CFU · mL⁻¹, the max concentration of FC was 1.1 x 10⁶ CFU · (100 mL)⁻¹ and the max concentration of FS was 1.1 x 10⁵ CFU · (100 mL)⁻¹. The FC/FS ratios were mostly over 2 which suggested that the main fecal source in groundwater was human feces. In addition, PCR-DGGE contrastive analysis of Bacteroides communities showed that the similarities between groundwater samples and human feces were in range of 7. 1% -69. 1% , and the similarity of the groundwater sample from Laolongdong underground river outlet was 69.1% . Bacteroides community similarities between groundwater samples and swine feces were in range of 1.1%-53.4%, and the similarity of Laolongdong underground river outlet was merely 1.5%. The similarity data implied that groundwater contamination resulted mainly from human feces, swine feces contamination composed part of animals' fecal contamination, and other animals' feces participated too. Furthermore, sequencing results of PCR-DGGE products revealed that most Bacteroides in groundwater originated from human intestinal tract and human feces.

To analyze the influencing factors of karstification and karst carbon cycle, a simulation experiment was carried out and 6 soil columns were designed. The results showed that the content of H2O4, hydrodynamic condition and thickness of the soil had important influence on karstification and karst carbon cycle. For the soil columns which were covered by the same thickness of soil, the concentrations of Ca2+ + Mg2+ and SO4(2-) followed the order of B20-2 > B20-1 > B20-3, B50-2 > B50-1 > B50-3. This meant that input of H2SO4 enhanced the karstification and increasing infiltration water had significant dilution effect on the chemical properties. For the soil columns with different thickness of soil but with the same slag pile and hydrodynamic conditions, the concentrations of Ca2+ + Mg2+ and SO4(2-) followed the order of B50-1 > B20-1, B50-2 > B20-2, B50-3 > B20-3. It was demonstrated that more carbonate rock was dissolved under the thick soil columns. In addition, the net consumption of CO2 mainly depended on the content of H2SO4 in this experiment due to slight contribution of H2CO3 to carbonate rock dissolution. More content of H2SO4 brought about less net consumption of C02, but B50-2 was an exception. Organic matter and other nutrients might be input into deep soil with the slag pile, and they promoted the production of soil C)2. Therefore, more CO2 was consumed due to the increased contribution of H2CO to karstification.

Objective: To study the association between vitamin D status and bone mineral density(BMD) in urban postmenopausal women in Beijing., Methods: A total of 400 community-dwelling women older than 60 (median age 67.8 years) were selected randomly from 17 community of 3 districts in Beijing by cluster sampling method from May to July, 2008. Serum 25(OH)D concentrations were measured by radioimmunoassay, using the DiaSorin kit, America. The objects were assigned into four groups: vitamin D deficient group (group A, 25(OH)D < 25 nmol/L), insufficient group(group B, 25 nmol/L < 25(OH)D < or = 50 nmol/L), normal group (group C, 50 nmol/L < 25(OH)D < or = 75 nmol/L) and enough group (group D, 25(OH)D > 75 nmol/L). The BMD at total, lumbar spine (L(2-4)) and proximal femur were measured by dual-energy X-ray absorptiometry (DEXA)., Results: The mean concentration of serum 25(OH)D concentration was (36.0 +/- 14.6) nmol/L, BMD at total and proximal femur were respectively (0.829 +/- 0.090) and (0.679 +/- 0.106) g/cm(2). The mean BMD at total of group A, B, C + D were respectively (0.811 +/- 0.077), (0.825 +/- 0.088) and (0.864 +/- 0.112) g/cm(2)(F = 16.93, P < 0.01), and BMD at proximal femur of group A, B, C + D were respectively (0.666 +/- 0.107), (0.673 +/- 0.099) and (0.725 +/- 0.117) g/cm(2)(F = 18.36, P < 0.01). The 25(OH)D level was positively correlated with BMD at total body and proximal femur (r values were 0.17 and 0.18, both P values < 0.05)., Conclusion: Vitamin D status was highly correlated with BMD at lumbar spine (L(2-4)), proximal femur, pelvis and limbs for the postmenopausal older women.