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To understand the characteristics of community structure and spatial distribution of small mammals in agricultural area of Yunnan Province, a systematic investigation was carried out in 104 quadrats of 25 regions in Yunnan Province from August 2010 to April 2018 by rat trap night method. The spatial variation of community characteristics along environmental gradients was analyzed by community ecological indicators. The results showed that a total of 3240 small mammals were captured and cold be classified into 42 species in 21 genera, 9 families, and 4 orders. The largest number of small mammal was rodents, dominated by Apodemus chevrieri and Rattus tanezumi. The 25 regions were clustered into three classes. The altitudinal distribution of small mammals was similar to the latitudinal distribution in agricultural areas. The number of species was relatively less in the low latitude and altitude range, with Rattus spp. and Mus spp. as the dominant species. In the high latitude and altitude region, the dominant species changed into Apodemus, Niviventer and Eothenomys. With the increases of altitude, the diversity index showed unimodal distribution, with the highest species diversity occurred in the mid-altitude area. The diversity index of small mammal showed the "V" type pattern in longitude, being the highest in the 98°-99° E gra-dient zone. At the latitude level, it showed an overall upward trend from south to north. Results from the GAM analysis showed that the degree of influence on the small animals in the agricultural area was in order of longitude, altitude and latitude. The similarity analysis in the composition of small mammals showed that the moderate similarity occurred in the adjacent gradient zone, and the highest similarity occurred in middle altitude zone, middle latitude zone, and low longitude zone. The farther the distance between different gradient zones, the lower the similarity of community structure. There was high spatial heterogeneity in different dimensions of small mammals' community structure in Yunnan Province. The geographical distribution trend of species diversity showed different distribution patterns across environmental gradients. [ABSTRACT FROM AUTHOR]

To understand the characteristics of community structure and spatial distribution of small mammals in agricultural area of Yunnan Province, a systematic investigation was carried out in 104 quadrats of 25 regions in Yunnan Province from August 2010 to April 2018 by rat trap night method. The spatial variation of community characteristics along environmental gradients was analyzed by community ecological indicators. The results showed that a total of 3240 small mammals were captured and cold be classified into 42 species in 21 genera, 9 families, and 4 orders. The largest number of small mammal was rodents, dominated by Apodemus chevrieri and Rattus tanezumi. The 25 regions were clustered into three classes. The altitudinal distribution of small mammals was similar to the latitudinal distribution in agricultural areas. The number of species was relatively less in the low latitude and altitude range, with Rattus spp. and Mus spp. as the dominant species. In the high latitude and altitude region, the dominant species changed into Apodemus, Niviventer and Eothenomys. With the increases of altitude, the diversity index showed unimodal distribution, with the highe-st species diversity occurred in the mid-altitude area. The diversity index of small mammal showed the "V" type pattern in longitude, being the highest in the 98°-99° E gra-dient zone. At the latitude level, it showed an overall upward trend from south to north. Results from the GAM analysis showed that the degree of influence on the small animals in the agricultural area was in order of longitude, altitude and latitude. The similarity analysis in the composition of small mammals showed that the moderate similarity occurred in the adjacent gradient zone, and the highest similarity occurred in middle altitude zone, middle latitude zone, and low longitude zone. The farther the distance between different gradient zones, the lower the similarity of community structure. There was high spatial heterogeneity in different dimensions of small mammals' community structure in Yunnan Province. The geographical distribution trend of species diversity showed different distribution patterns across environmental gradients.

Objective: To explore the predictors on the abundance of Rattus (R.) tanezumi in households of commensal rodent plague foci., Methods: Thirty natural villages that experienced previous plague cases in Lianghe county, Yunnan province, were selected followed by random selection of 20 households in each village through computer technique. Live traps were set in households to capture small mammals which were then identified to species in the field according to their morphological features. Data on potential factors for abundance of R. tanezumi were collected through questionnaires and field observation and were coded and computerized using EpiData software and further analyzed by hurdle regression model under R software., Results: A total of 166 rodents (133 R. tanezumi and 33 Suncus murinus) were captured., Results: from final multilevel hurdle regression model showed that the likelihood of R. tanezumi captures increased by 1.67- to 2.76-fold in households belonged to Dai ethnic families that stored foodstuff in metal pail, often raising dogs, and having adjacent houses. The number of R. tanezumi captures increased by 2.18-fold in the villages where over 80% of the households would raise chickens. In the villages with communal latrine, the likelihood and the number of R. tanezumi capture increased 1.93-fold and 2.38-fold, respectively. While the likelihood of R. tanezumi captures would reduce by 45% - 61% in those households where there were cats and cattle being raised and maize grown in the village. The number of R. tanezumi captures would reduce by 63% in the households where there were outside toilet., Conclusion: The abundance of R. tanezumi seemed to be closely related to the ecological environment factors. Programs on plague control and prevention should relate to ecological factors that influencing the abundance of R. tanezumi.

Aim: To prepare the monoclonal antibody of Pla with recombinant Pla (rPla) by hybridoma cell technology, which will lay the foundation for related research work., Methods: Purified rPla was collected by washing repeatedly with urea, and BALB/c mice were immunized by them. Hybridoma cells were achieved by Sp2/0 cell fusion with mouse spleen cells from successfully immunized mice. Monoclonal antibody was screened by indirect ELISA and Western blots with rPla, natural crude Pla and GST respectively., Results: Three strains of hybridoma cells (named 15B8, 14H4 and 19A4 respectively) which secreted stably the monoclonal antibody of Pla were obtained. Their subclasses were IgG2a and IgG1 in heavy chains and κ chains in light chains. The ELISA titers of ascites were 10(6); respectively.Three of monoclonal antibody can react with natural crude Pla tested by western blots., Conclusion: Monoclonal antibody of natural Pla of Yersinia pestis were successfully got, which has laid the foundation for further study of the Pla protein and development diagnosis reagent.