1. [Study on antitumor drug-induced apoptosis in human cancer cells by terminal deoxynucleotidyl transferase assay].
- Author
-
Tong T, Sun H, and Liu L
- Subjects
- Bronchi cytology, Carboplatin pharmacology, Cell Line, Transformed, Cystadenocarcinoma, Papillary pathology, DNA Nucleotidylexotransferase, Epithelial Cells cytology, Female, HL-60 Cells pathology, Humans, Ovarian Neoplasms pathology, Proto-Oncogene Proteins c-bcl-2 biosynthesis, Proto-Oncogene Proteins c-bcl-2 genetics, RNA, Messenger genetics, Antineoplastic Agents pharmacology, Apoptosis drug effects, Cisplatin pharmacology
- Abstract
Objective: Considerable evidence has showed that apoptosis is involved in both cancer development and inhibition. A new assay (terminal deoxynucleotidyl transferase assay, TdT assay) was recently reported to have advantages in the detection of apoptosis. In this study, this assay was used to investigate antitumor drug-induced apoptosis in human cancer cells., Methods: TdT assay, DNA gel electrophoresis, electron and light microscopy were used to observe apoptosis., Results: Our results showed that cisplatin-induced apoptosis in both HL-60 and SV40T-transformed human bronchial epithelial cells was detected with a good dose and time response. The occurrence of the apoptosis was preceded by the decrease of bcl-2 mRNA expression. With TdT assay, apoptotic cells were observed in the ovarian tumor of patients treated with carboplatin., Conclusion: TdT assay may be applicable to moniter apoptosis in human cancers induced by chemotherapy, and to evaluate tumor cell response during treatment.
- Published
- 1997