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An inversion heterozygote has been found in the fourth stage larvae of the laboratory line of Shanghai Anopheles sinensis in our laboratory, the frequency of occurrence of the heterozygote being 2 to 5%. The characteristic inversion loop formed in the midst of chromosome 3R together with the break points arises from 26C to 30A segment of the chromosomes. This inversion paracentric is the first record in the literature available and may be of significance as a genetic marker in studying the inter-specific variation of Anopheles sinensis.