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Copyright of Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao is the property of Chinese Journal of Applied Ecology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The ultrasonic extraction of chlorophenol in textiles was carried out by potassium hydroxide (KOH) solution because of the shortcomings of the existing detection methods for chlorophenol in textiles. A method based on gas chromatography-mass spectrometry (GC-MS) was established for the simultaneous determination of 19 chlorophenols in textiles. Pretreatment conditions of extraction were optimized. Textile samples were ultrasonically extracted with 2.0 mol/L KOH solution in 2.0 h under 70 °. The solution was acetylated by acetic anhydride to produce chlorinated phenol acetate and the target was extracted by hexane liquid/liquid extraction. The results show that the calibration curves are linear between the peak area and the concentration of 19 chlorophenol acetates in the range of 1-40 µg/L, with correlation coefficients greater than 0.995. The limits of detection (LOD) are 0.006-0.025 mg/kg and the limits of quantitation (LOQ) are 0.020-0.082 mg/kg. The average recoveries range from 82.7% to 113.3% at three different spiked levels of 0.05, 0.50 and 1.00 mg/kg, respectively, with relative standard deviation (RSD, n=6) less than 15.0%. The method is simple, rapid and accurate, which is suitable for the determination of various chlorophenols in textiles. [ABSTRACT FROM AUTHOR]

Organic pollutant 2,4,6-trichlorophenol (2,4,6-TCP) is commonly found in anaerobic environments such as sediments and groundwater aquifers. To investigate the ability of the anaerobic consortium XH-1 to degrade 2,4,6-TCP, we established anaerobic incubations using 2,4,6-TCP as the substrate and inoculated the incubations with XH-1. Additional subcultures were established by amending with intermediate product 4-chlorophenol (4-CP) or phenol as the substrate. The transformation products of 2,4,6-TCP were analyzed and determined using high-performance liquid chromatography (HPLC). Microbial community structure and key microbial groups involved in the degradation of 2,4,6-TCP were analyzed based on 16S rRNA gene high-throughput sequencing. The results showed that the initial 122 μmol·L -1 2,4,6-TCP was completely transformed after a 80-day incubation at a rate of 0.15 μmol·d -1 . 2,4-dichlorophenol (2,4-DCP), 4-CP and phenol were identified as the intermediate products. All intermediate products generated from 2,4,6-TCP transformation were completely degraded after being incubated for 325 days. The main microbial groups responsible for the reductive dechlorination of 2,4,6-TCP might be the organohalide respiring Dehalobacter and Dehalococcoides . The subsequent reductive dechlorination of 4-CP to phenol was likely driven by Dehalococcoides . The cooperation between the organohalide respiring bacteria, Syntrophorhabdus and methanogens ( e.g. Methanosaeta and Methanofolis ) was responsible for the complete degradation of 2,4,6-TCP.