1. [Pilot-scale production of recombinant plasmid pUDK-HGF].
- Author
-
Hu C, Wang Y, Lu Y, Cheng X, Liu L, Zhang T, and Zhang Q
- Subjects
- DNA, Superhelical isolation & purification, Escherichia coli genetics, Escherichia coli metabolism, Fermentation, Genetic Vectors genetics, Hepatocyte Growth Factor biosynthesis, Humans, Pilot Projects, DNA, Recombinant biosynthesis, Hepatocyte Growth Factor genetics, Plasmids isolation & purification
- Abstract
pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.
- Published
- 2011