1. [Effects of angiotensin-(1-7) on oxidative stress and functional changes of isolated rat hearts induced by ischemia-reperfusion].
- Author
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Liao XX, Guo RX, Ma H, Wang LC, Chen ZH, Yang CT, and Feng JQ
- Subjects
- Animals, Heart physiology, In Vitro Techniques, Male, Myocardial Reperfusion Injury metabolism, Random Allocation, Rats, Rats, Sprague-Dawley, Superoxide Dismutase metabolism, Angiotensin I pharmacology, Heart drug effects, Myocardial Reperfusion Injury physiopathology, Oxidative Stress drug effects, Peptide Fragments pharmacology
- Abstract
Objective: To investigate the effects of angiotensin (Ang)-(1-7) on oxidative stress and functional changes in isolated rat hearts with myocardial ischemia-reperfusion (IR) injury., Methods: IR injury was induced in isolated rat hearts with the Langendorff' apparatus. The left ventricular systolic pressure (LVSP) of the rat heart was measured using a pressure transducer. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in the myocardium were detected using commercial kits., Results: Myocardial ischemia (15 min) and reperfusion (30 min) significantly increased myocardial levels of MDA, and reduced the SOD activity and LVSP (P<0.05). Pretreatment with Ang-(1-7) at 1.0 nmol/L 30 min before ischemia obviously inhibited IR-induced MDA increment and lowering of SOD activity and LVSP. Pretreatment of the rats with intraperitoneal injection of 5 mg/kg indomethacin 1 h before isolation of the heart markedly antagonized the effect of Ang-(1-7) on MDA production, SOD activity and LVSP., Conclusion: Angiotensin-(1-7) can inhibit IR injury-induced oxidative stress and decrease in cardiac contractile function in isolated rat hearts. The mechanism underlying the effect of Ang-(1-7) may be associated with increased secretion of prostaglandin.
- Published
- 2008