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Due to the large terrain fluctuation and drop in Qianshuta well field, the layout of the ground large loop transient electromagnetic method detection device is limited. In order to find out the distribution range of goaf under the second Shanjing line of oil and gas pipeline in this area, numerical simulation and field test of small loop transient electromagnetic method were carried out to analyze the feasibility of ground detection, and optimized parameters were applied to the field. The results show that the numerical simulation confirms that the 3 m×3 m small loop transient electromagnetic method can effectively detect the goaf with a depth of more than 200 m and a mining height of more than 1 m, and has a more obvious effect on the water-filled goaf with low resistance; the field test adopts the small loop transient electromagnetic method with optimized parameters such as the length of the transmitting wire frame 3 m×3 m, the number of stacking times of 15, the transmitting frequency of 16 Hz, and more than 8 A transmitting current, which can achieve the purpose of effective detection and verify the correctness of the numerical simulation; a total of 5 abnormal areas were delineated and explained in the No.3 coal seam, which were inferred to be gobs or burned rock voids. The reliability of the small loop transient electromagnetic detection method is fully verified.

Objective: To determine the expressions of ryanodine receptor type 1 (RyR1) and Cav1.3 L-type calcium channel (Cav1.3) in the vaginal smooth muscle cells of castrated rats and investigate the correlation of RyR1 and Cav1.3 with estrogen in female sexual dysfunction., Methods: Forty female SD rats of 8 weeks were randomly divided into Groups A (2-week sham operation), B (4-week sham operation), C (2-week castration) and D (4-week castration). Two and 4 weeks after surgery, the serum estradiol level was determined with the automated immunochemiluminescence system and the expressions of RyR1 and Cav1.3 in the vaginal smooth muscle were detected by immunohistochemistry and RT-PCR. Gray scale ratio was used to represent the mRNA expression levels of RyR1 and Car1.3, and the optical density value to denote their protein expression levels., Results: Serum estradiol was significantly decreased in Group C ([0.210 +/- 0.026] nmol/L) as compared with A ([0.505 +/- 0.053] nmol/L) (P < 0.01), and so was it in Group D ([0.130 +/- 0.031] nmol/L) in comparison with B ([0.476 +/- 0.058] nmol/L) (P < 0.01). RyR1 and Cav1.3 were expressed in all groups. The mRNA expressions of RyR1 and Cav1.3 were significantly reduced in Group C (0. 680 +/- 0.073 and 0.580 +/- 0.043) as compared with A (0.950 +/- 0.064 and 0.870 +/- 0.019) (P < 0.01), as well as in Group D (0.220 +/- 0.032 and 0.190 +/- 0.020) in comparison with B (0.890 +/- 0.072 and 0.820 +/- 0.021) (P < 0.01). The protein expressions of RyR1 and Cav1.3 were significantly down-regulated in Group C (96.67 +/- 7.75 and 87.97 +/- 6.96) as compared with A (123.69 +/- 10.66 and 106.46 +/- 8.04) (P < 0.01), and so were they in D (86.45 +/- 8.16 and 69.43 +/- 8.30) in comparison with B (109.31 +/- 9.87 and 97.38 +/- 7.56) (P < 0.01)., Conclusion: Both RyR1 and Cav1.3 were expressed in the vaginal smooth muscle cells of the rats, and estrogen might be involved in the regulation of female sexual reaction by acting on the expressions of RyR1 and Cav1.3.