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To effectively control the hidden danger of coal spontaneous combustion caused by air leakage in shallow buried coal seam in Shendong Mining Area, taking the upper and lower coal seams of Huojitu Well 12 in Daliuta Coal Mine as the research object, the precision air leakage and fire prevention project demonstration was carried out by means of three-dimensional curtain grouting. The scheme calculates the grouting distance and designs the grouting layout using geological profiles to ensure that the grouting body is not affected by mining activities. Different geological conditions are addressed through the design of pilot boreholes, grouting boreholes, and monitoring boreholes. In accordance with the actual conditions of Huojitu Well, a total of 104 boreholes are constructed, with a curtain body length of approximately 1 020 m and a height of about 40 m. Utilizing the segmented ascending grouting method, the grouting height is set at 72 m for the working face cutting eye of the Well 12 lower coal seam, and 12 m for the 205 coal transport section of the Well 12 upper coal seam, with a grouting width of 15 m. The total grouting volume is estimated at 169 569.1 m3. Through the solid curtain grouting, the air leakage of working face 205 in Well 12 lower coal seam is reduced by about 1 000 m3/min and that of working face 207 in Well 12 lower coal seam is reduced by nearly 800 m3/min compared with the same conditions; the effect of plugging leakage air is obvious, and the hidden danger of spontaneous combustion of coal in the working face of Well 12 coal seams is effectively reduced.

In this work, the coordination chemistry of the uranyl ion(\begin{document}${\mathrm{UO}}_2^{2+} $\end{document}) with N, N-dimethyl-diglycolamic acid(HDMDGA, HLI) and N, N-diethyl-diglycolamic acid(HDEDGA, HLII) has been systematically studied through thermodynamic and structural analysis, by means of both potentiometric and spectroscopic titration, as well as Raman spectroscopy and X-ray diffractometry. The protonation constants of L−(L=LI, LII) and the stability constants of the uranyl complexes were determined by potentiometric and spectroscopic titrations in 1.0 mol/L NaClO4, with the molar absorption spectra of all species deconvoluted in the process of the latter titration. Besides, the standard Raman spectra of various complexes were confirmed by Raman spectroscopic titration. Furthermore, the single crystal of compound \begin{document}${\mathrm{UO}}_2{\mathrm{L}}^{{\mathrm{II}}}_2 $\end{document} was obtained by slow evaporation of aqueous solution containing uranyl ion and LII ligands at room temperature. X-ray diffraction analysis shows a monoclinic crystal system in P21/c space group, with lattice parameters: a=15.5096(6) Å（1 Å=0.1 nm）, b=7.7934(3) Å, c=11.3732(4) Å, α=90°, β=97.388(4)°, γ=90° and Z=2. Although both ligands show a similar structure and protonation constant, the different values of stability constant in aqueous solution and the different bond length of U-O in two complexes indicate the distinct coordination ability for two ligands with \begin{document}${\mathrm{UO}}_2^{2+} $\end{document}, where HDEDGA performs better. Like the crystal structure of \begin{document}$ {\mathrm{UO}}_2{\mathrm{L}}^{{\mathrm{I}}}_2$\end{document}, the ligand LII in the \begin{document}${\mathrm{UO}}_2{\mathrm{L}}^{{\mathrm{II}}}_2 $\end{document} complex coordinates with \begin{document}${\mathrm{UO}}_2^{2+} $\end{document} in a tridentate manner, with three oxygen atoms(amide oxygen, ether oxygen and carboxylic acid oxygen) coordinating with the linear \begin{document}${\mathrm{UO}}_2^{2+} $\end{document} at the equatorial plane. In addition, the diffuse reflectance spectra of the \begin{document}${\mathrm{UO}}_2{\mathrm{L}}^{{\mathrm{II}}}_2 $\end{document} crystal show characteristics significantly different from the absorption spectra of the corresponding complex in the aqueous solution. Meanwhile, the Raman shift of the characteristic peak of uranyl ion between hydrated uranyl ion and the 1∶1 complex(U∶ LII) is significantly larger than that between 1∶1 and 1∶2. Therefore, it is speculated that the structure of the 1∶2 complex in aqueous solution is supposed to be different from that in the solid crystal.

This is an article in the field of process mineralogy. A quartz vein type gold deposit in Shandong has a gold grade of 2.51 g/t. In order to further develop the deposit and study the beneficiation process, the process mineralogy of the mineral was carried out. The technical methods involved in this study include multi-element chemical analysis, chemical phase analysis, optical microscope identification, X-ray diffraction (XRD) analysis, automatic quantitative mineral analysis (BPMA), scanning electron microscope energy spectrometer (SEM-EDS) analysis, etc. The results show that: (1) most of the gold minerals in the ore are silver-gold, and a small amounts of gold, silver and natural gold are occasionally found; (2) The embedding relationship between gold minerals and sulfides such as pyrite in the ore is very close. Pyrite is the main carrier mineral of gold minerals, and the proportion of single and exposed gold in the ore is high, which is 58.96%. The proportion of gold encapsulated in sulfides such as pyrite is 39.84%, and the proportion of gold encapsulated in gangue minerals is 1.20%; (3) The particle size distribution of gold minerals in the ore is uneven, and the overall particle size is relatively fine. Gold particles with a particle size larger than 0.020 mm can be seen in the ore, with an occupancy rate of 22.08%. Gold minerals with a particle size less than 0.020 mm have an occupancy rate of 77.92%, with an occupancy rate of 24.83% for micro gold minerals with a particle size less than 0.005 mm and 0.35% for sub micron sized gold minerals with a particle size less than 0.001 mm.

Aiming at the problems such as low construction efficiency and high labor intensity in the process of lowering large diameter casing through the broken stratum of complex roof high level drilling in coal mine, a large diameter casing lowering device is designed. This paper introduces the design of large diameter casing lowering device, describes in detail the design process of front inner support assembly, back inner support assembly, cylinder block, slip and other key parts, and describes the technological steps of casing lowering by drilling rig. During the field industrial test, 68 casings with a diameter of ϕ245 mm and a length of 1.5 m were run in 4 directional boreholes with a tilt angle of 17°, totaling 102 m. The large diameter casing lowering device is sensitive and reliable, and can meet the functional requirements of mechanized large diameter casing lowering in complex roof high level drilling.

The deformation and failure characteristics of coal around gas drainage boreholes in deep soft and low permeability coal seams affect coal seam gas pre-drainage. Based on the condition of non-uniform stress field, the mechanical model of borehole disturbed coal mass was developed, the analytical solutions of stress, strain and displacement in the damaged zone, plastic zone and elastic zone of borehole disturbed coal mass were deduced, the influence law of factors such as lateral pressure coefficient, load condition, cohesion and hole expanding behavior on the “three zone” distribution of disturbed coal mass were analyzed, and the reliability of the theoretical model was verified through engineering examples. The results show that under the condition of non-uniform stress field, the plastic zone and damaged zone of disturbed coal mass are elliptical distribution. With the increase of lateral pressure coefficient, the length of the upper and lower wings of the plastic zone and damaged zone of disturbed coal mass becomes larger and larger, and the radius of the plastic zone and damaged zone in the direction of smaller stress is greater than the radius of the two zones in the direction of large stress. The radius of plastic zone and damaged zone of coal mass increases with the increase of vertical load, and decreases with the increase of initial cohesion and residual cohesion. The influence of vertical load on its shape can be ignored. When the borehole diameter is expanded from 0.1 m to 0.5 m, the coal mass 0−1.0 m away from the borehole center produces a strong disturbance, the coal mass 1.0−4.6 m produces a weak disturbance, and the coal mass after 4.6 m has almost no influence. Through the field example of No.16032 bottom pumping roadway hydraulic reaming in the Guhanshan coal mine, it is observed that the disturbed coal mass in the reaming section has a high degree of damage. Based on the coal output, the reaming diameter is deduced to be 1.5 m, and then the deformation and damage characteristics of drilling reaming coal mass are obtained through theoretical calculation and numerical simulation respectively. The two are in good agreement, so as to verify the reliability of the theoretical model.

In view of the limitations of directional drilling technology and equipment for underground gas drainage in coal mining areas, such as low degree of automation, low drilling ratio of target formation, slow drilling rate of rock formation, and small diameter, the research of rotary geosteering drilling technology and equipment underground coal mine has been carried out, with the core technical problems has overcame such as wireless remote control operation of directional drilling rigs, mechanically automatic drill pipe addition, real-time parameter monitoring and fault diagnosis, coal and rock identification while drilling, and trajectory rotation control, ZDY25000LDK electro-hydraulic controlled directional drilling rig, BLY800/12 mud pump truck with high-pressure and large-volume, YSG(A) Mine geosteering MWD system by dynamic azimuth gamma, ϕ133 mm hydraulic push type rotary steering drilling system and supporting drilling tools have developed, with the maximum torque was 25 000 N·m, the automatically add drill pipe time was less than 55s of the directional drilling rig, the rated flow was 800 L/min of the mud pump truck, the natural gamma measurement error was less than ±5% and the stratum exploration distance was ≥0.5 m of the geosteering MWD system, the applicable speed range was 80−200 r/min and the deflection force was 1.4 t of the rotary steerable drilling system; geosteering drilling technology and rotary steerable drilling technology for near horizontal hole has developed with the technical characteristics and process flow are introduced in detail. The field test was carried out in Tangjiahui Coal Mine in Inner Mongolia. 4 directional drillings were completed, with the maximum drilling depth was 820 m, the total footage was 2 419 m, the directional drilling diameter was 172 mm, the drilling efficiency was increased 25% in coal seam and 30% in rock, which realized the directional drilling from “geometric steering” to “rotary geosteering” underground coal mine, the intelligent level and drilling trajectory quality of underground directional drilling in coal mine has been improved, and provided technical equipment support for efficient underground gas drainage and intelligent mine construction.

Part of a gold deposit belongs to altered type gold deposit. The gold bearing alteration zone is composed of sericite, silicification and pyritization caused by hydrothermal alteration of granite. Most of the ore bodies are hosted in pyrite sericite and some in pyrite sericite granite. Using optical microscope, mineral analysis system (BPMA), scanning electron microscope and energy spectrum, the process mineralogical characteristics of minerals were analyzed. It was found that sulfide ore was the main ore and the depth of oxidation zone was very shallow. The gold grade is 5.23 g/t. The main gold minerals are natural gold and silver gold. The main metal minerals are pyrite, pyrrhotite, galena, chalcopyrite, sphalerite and other sulfide minerals and magnetite and other oxide minerals. Gangue minerals mainly include quartz, sericite, feldspar, etc. The size of gold is fine, all of which are below 37 μm. According to the occurrence state of gold minerals, the content of gold bearing minerals existing in monomer form and coexisting with pyrite is 83.18%, which is easy to recover; the content of gold minerals associated with sericite, quartz and magnetite is 16.82%, which is the main reason for the low gold recovery rate in flotation process.

This article was aimed at a certain tellurium-bearing gold ore and its samples after alkaline leaching. Through the process mineralogical analysis of two samples, the elemental composition, mineral composition and minerals of the ore in the sampleswere respectively determined. The characteristics of the embedding, the occurrence state of the target element, the particle size distribution of the target mineral, etc. were reported. The research results showed that the presence of tellurium-bearing gold ore in the raw ore affectedthe leaching rate of gold during the leaching process. The alkaline leaching pretreatment process can oxidize the tellurium-bearing gold ore; the fine-grained minerals in the raw ore were oxidized and decomposed into fine particles during the alkaline leaching process. Granular minerals,during the alkaline leaching process, pyrite and tellurium-bearing gold ore were oxidized to release gold minerals. It is shown that the alkaline leaching pretreatment process has obvious effects on the treatment of tellurium-bearing gold ore, and has guiding significance for the formulation of the process flow of tellurium-bearing gold ore.

For simulating the actual working conditions, a series of gradient ratio tests of geotextile filtering clay are completed by using an improved gradient ratio permeameter that can apply vertical load. The tests are carried out under the condition of the critical hydraulic gradient (1.0) and hydraulic gradient equal to 2.0, respectively, with the 4th layer of clay soil in Shanghai and the short fiber needle punched non-woven geotextile. The variation of the filtration performance of geotextile with hydraulic gradient and normal stress is studied by changing the vertical load. The results show that the hydraulic gradient and normal stress are the important factors affecting the clogging characteristics and the permeability of geotextile filter. The gradient ratio of the geotextile filter clay increases with the increase of the vertical load, but there is no impermissible clogging when it is stable. Under a certain load, the coefficient of permeability decreases with time and then tend to stabilize. The stabilized coefficient of permeability decreases with the increase of the vertical load. The hydraulic gradient from 1.0 to 2.0 also causes the increase of the gradient ratio and the decrease of the permeability. The test results better reflect the filtration effect of the geotextile filter under the actual working conditions, which can provide references for similar engineering design.

In order to evaluate the effect of complex fermentation of different yeasts and Lactobacillus plantarum on the fermentation quality of Xinhui citrus, three kinds of yeasts and Lactobacillus plantarum were used as raw materials for compound fermentation of fresh fruit pulp of Xinhui citrus. The changes of yeast viable count, total sugar content, pH and total acidity in fermentation broth were tracked during the fermentation process, and the bioactive substances and antioxidant activities of Xinhui citrus fermentation liquor in different fermentation systems were compared. The results showed that all the three strains of yeast could grow normally in Xinhui citrus, but the growth rate of Saccharomyces cerevisiae (Sc) and Kluyveromyces marxianus (Km) in Xinhui citrus was higher than that of Kazachstania unispora (Ku). Fermentation could increase the contents of total flavonoids, polyphenols and γ-aminobutyric acid of Xinhui citrus fermentation liquor and its antioxidant activity in vitro, but it could partially degrade crude polysaccharides. The contents of crude polysaccharide and γ-aminobutyric acid were the highest in the yeast fermentation system, which could reach 4.37 g/kg and 82.85 mg/kg. The content of total flavonoids in the yeast fermentation system was the highest, which was 1.54 g/kg. In addition, the hydroxyl radical scavenging rate and total reducing power of K. marxianus fermentation broth were 82.48% and 1.09, respectively, which were significantly higher than those of other yeast fermentation(P

The research progress of numerical simulation of parabolic trough solar collector was introduced. Especially the numerical simulation studies of the internal flow field in the collector tube and the external flow field of the concentrator were reviewed. In the study of the internal flow field in the collector tube, the type of heat transfer fluids and the characteristics of optical concentration have a significant effect on the heat collection performance and thermal stress distribution, especially in the water/steam medium heat collection loop. The unique gas-liquid two-phase flow superposition of the non-uniform heat flux distribution outside the tube may lead to serious thermal stress bending deformation of the collector tube. In the study of the external flow field of the concentrator, due to the high actual wind speed and the low structural strength of the ultra-thin lens, the concentrator is subjected to wind load deformation, resulting in the loss of optical efficiency, and even leading to the failure of the parabolic trough collector structure, which directly affects the normal operation of the whole collector field.

Geogrid reinforced soil slope is a new type of slope supporting structure, which is of great significance for improving the slope stability, saving construction land and preserving the ecological environment. In order to provide the optimal design solution of the reinforced soil slope for high fill slopes, a case study is conducted on the No.6 slope at the northwest corner of the airport runway. Three design schemes with different slope ratios are carried out firstly based on the geological conditions of the slope and the field situation of the high fill slope. Secondly, the simplified Bishop method, Spencer wedge method and Morgenstern price method are used to calculate the stability factors of the reinforced slopes under the natural, rainstorm and earthquake conditions. Finally, the deformation characteristics of the slope and tensile force in reinforcements are investigated based on the finite element method under the natural conditions. The results show that all the proposed design schemes can meet the requirements of slope stability under different working conditions. A zigzag distribution of the tensile force in reinforcements in the multi-stage reinforced soil slope is observed along the slope height, and the maximum axial force of reinforcement increases abruptly at the toe of each slope. Compared with the design scheme of the gentle reinforced soil slope with the slope ratio of 1∶1.5, the reinforced retaining wall with the slope ratio of 1∶0.25 shows obvious advantages in reducing the slope height, consumption of geosynthetics, filling and excavation amount as well as slope protection area. The key issues including the slope stability, construction cost and duration are comprehensively considered, and the design scheme of the reinforced earth wall is reasonable.

With the development of technology，the high precision transmission equipment has higher and higher requirements on gear. In the gearbox of main reduction helical cylindrical gear as the research object，by using Romax software，the gearbox model is established，combining the theory of microscopic parameters optimization of cylindrical bevel wheel，the transmission error，distribution of load per unit length of gear tooth surface and the contact spot are taken as the optimization objectives for microscopic gear modification，an omnidirectional modification method combining helical modification and tooth profile modification is proposed. By comparing the goal of the optimization of the gear parameters before and after modification，reduce the optimized transmission error and tooth surface load distribution more uniform，good contact spots，improve the gear meshing，improve the service life of the gear.

As a heat storage method with high heat storage density, long storage time and long transportation distance, the chemical heat storage has been widely studied in recent years. This article firstly summarized the basic principles and research progress of several existing solid-gas two-phase chemical heat storage reaction systems, mainly involving the metal hydroxide reaction, metal oxide reaction, carbonate reaction, and hydration salt reaction. Then, according to the moving state of solid phase reactant in the reactor, the reactor was divided into three types: the fixed bed reactor, fluidized bed reactor and moving bed reactor. Moreover, their research progress was summarized respectively. Finally, based on the current deficiencies in the research of chemical heat storage, the future development trend of chemical heat storage was analyzed. At present, the storage still needs further research, and there are still technical difficulties in strengthening and matching the chemical reaction rate and heat transfer. The cyclic stability of chemical heat storage under long-term operation condition still needs to be further explored.

In view of the thermal management of power batteries, the new liquid-cooling structure of 18650 cylindrical lithium ion battery array and its cooling effect were studied. An immersion cooling method combined with a checkerboard topology shunt structure was proposed. And by combining the shape and array arrangement of the lithium ion battery, a simple and reasonable immersion cooling topology based on the battery array was formed. Under this new cooling structure, the single model and module model of the battery array were established. Through the numerical simulation, the temperature distribution of the single and module models at every moment was analyzed, and the feasibility of replacing the module model with the single one was verified. On the basis of ensuring the calculation accuracy of the single model, numerical simulations on the layout of the inlet and outlet of the cooling channel were carried out. The results show that the same-side arrangement and the different-side one are almost the same in the pressure drop, but the same-side arrangement is superior to the different-side arrangement in terms of cooling effect. Moreover, with the increase of the heat generation of the battery and the extension of the heating time, the advantages of the arrangement of the inlets and outlets on the same side are more obvious.

In view of the existing Deepfake detection algorithms, such problems as low accuracy and poor interpretability are common.A neural network model combining the double attention was proposed, which used channel attention to capture the abnormal features of false faces and combined the location of spatial attention to focus the abnormal features.To fully learn the contextual semantic information of the abnormal part of the false face, so as to improve the effectiveness and accuracy of face changing detection.In addition, the decision-making area of real and fake faces was shown effectively in the form of thermal diagram, which provided a certain degree of explanation for the face exchange detection model.Experiments on FaceForensics ++ open source data set show that the detection accuracy of proposed method is superior to MesoInception, Capsule-Forensics and XceptionNet.

Improving the quality of on-grid solar power has attracted worldwide attention in recent years due to the natural intermittent and instability of solar irradiance. As an emerging technology, the photovoltaic/concentrated solar power (PV-CSP) hybrid technology is considered as one of the research focuses currently in solar energy field, due to various advantages compared with the PV-alone and CSP-alone technologies. This paper introduced PV, CSP technologies, and PV-CSP hybrid technology. Through the attention to the construction and operation of some typical commercial PV-CSP hybrid power plants, the application status of commercial PV-CSP hybrid power plants was analyzed, and the technical and economic research of PV-CSP hybrid system in recent years was summarized.

The low thermal conductivity for most of phase change materials is one of main obstacles in real application. Foam metals can be added into the phase change material to strength heat transfer process because of high thermal conductivity, high porosity and high specific surface area of foam metals. Based on the 3D W-P micro structure model of foam metal, the effect of porosity and pore diameter of foam metals on effective thermal conductivity of the foam metal based composite phase change materials were analyzed. The numerical simulation method was used to predict the thermal conductivity of foam aluminum-air and foam aluminum-water composites.The results show that the W-P model can be used to precisely predict the thermal conductivity of foam metals. The effective thermal conductivity of foam copper-paraffin composite was then analyzed. It is shown that the thermal conductivity of phase change material can be enhanced greatly by adding foam metals, and the thermal conductivity of paraffin has been increased 13 times when the porosity of foam copper is 97.57%. This study has reference value for thermophysical properties enhancement research on phase change materials.

Network densification was one of the main ways to deal with the challenge of supporting 1 000 times higher capacity in 5G networks. Wherein, a single node needs to configure and optimize more than 2 000 parameters. Therefore, novel self-organizing network (SON) would be the inevitable technique for wireless networking, where decision makers were enabled with the ability of perceiving the dynamic network situation and meanwhile, autonomously discovering as well as configuring network resources. With the aid of this promising technique, users' demands and network resources could be well matched and furthermore, the capacity of networks could be significantly improved. The trend of dense development in wireless communications was discussed. Then, the potential challenges and required new properties was analyzed when util-izing SON in ultra-dense wireless networks. Meanwhile, two typical use cases were depicted. Finally, for future ultra-densely deployed wireless networks, the possible development prospect and direction of SON were briefly presented.

One typical deployment scenario was described and several key techniques including channel bonding were introduced.More importantly,a two-tire dynamic spectrum access model was formulated from both the economic and technologic perspectives.In addition,a variety of dynamic resources sharing schemes were summarized.Furthermore,classic game theoretic models were discussed and consequently strategic design of dynamic spectrum access and intelligent network selection were introduced.Finally,a spectrum sharing scheme was proposed and the future of cognitive Wi-Fi 2.0 networks was looked to.

[Objectives] To clarify the effects of cold storage time on the survival, parasitism and reproduction of Bracon adoxophyesi Minamikawa, and thereby improve the low-temperature storage of this parasitic wasp. [Methods] The effect of keeping B. adoxophyesi at 10 ° for 30, 45, 60, 75, 90 and 105 d on adult survival rate, parasitism, oviposition and offspring development, were measured and compared. [Results] The survival rates of adults were 97.35% and 82.07% after 30 and 45 d cold storage, respectively. Survival declined with longer periods of cold storage to a minimum of 46.77% after 75 days. There was no significant difference in the number of hosts (Galleria mellonella Linnaeus) killed by, or the average mortality rate (79.00%-92.00%) of, female wasps that had been subject to 30-75 d cold storage compared to the control group (99.00%). However, cold storage significantly reduced the parasitism rate which was 25.00% after 30 days cold storage compared to 44.00% for the control. Cold storage increased the oviposition rate; the highest total number of eggs laid (78.60) was recorded after 30 d cold storage, followed by 60 d and 45 d (71.33 and 70.00, respectively), all of which were significantly higher than the control (47.80). Cold storage in did not affect hatching, pupation or adult emergence of progeny, but did decrease the ♀ : ♂ sex ratio of offspring. [Conclusion] Storing B. adoxophyesi at 10 °C for 30-60 d had no effect on the host paralysis rate, and increased the oviposition rate relative to the control. B. adoxophyesi should be kept in cold storage for no more than 60 d. [ABSTRACT FROM AUTHOR]

Open-pit mining of coal mines in arid and semi-arid areas destroys surface morphology, accelerates soil water loss, and the lack of water resources seriously affects the effect of vegetation restoration in open pit dump. Soil reconstruction technology and microbial remediation technology point out the direction to solve the water shortage dilemma of vegetation in open pit dump, but the relationship between plant root morphological characteristics and soil water use efficiency under soil reconfiguration and the important role of dark septum endophytic fungi (DSE) in soil reconfiguration are not well understood. The purpose of this study was to explore the root growth and water use of maize under soil layer reconstruction mode, as well as the mechanism of DSE. The study object was maize, and the soil layer reconstruction experiment was simulated in a greenhouse for 4 months. The experiment was arranged in three groups: blank control(N-CK), soil layer reconstruction without DSE inoculation(L-CK), soil layer reconstruction with DSE inoculation(L-DSE). The results showed that: ① Compared with blank control treatment, the total root length of maize root system under soil layer reconstruction treatment was reduced by 39.7%, the proportion of fine roots less than 0.3mm in the root system was increased by 8.86%, and the water use efficiency of deep (50−70 cm) soil was increased by 16.1%. ② Compared with the reconstructed soil layer without DSE inoculation, the proline content in leaves of maize inoculated with DSE increased by 69.8%, the number of root tips and specific root length in 30−50 cm soil layer increased by 106.6% and 38.5%, respectively. In addition, the water use efficiency in deep soil increased by 29.3% and 11.1%, respectively, compared with that of N-CK and L-CK. ③ Root tip number and fine root volume of the maize root system are the key root characteristics to achieve efficient use of soil water under the soil layer reconstruction model. The combined study showed that soil layer reconstruction was helpful to optimize the root structure of maize, increase the proportion of fine roots and the number of root tips, and improve the water use pattern of maize under water shortage environment, and DSE inoculation under the soil layer reconfiguration model could improve the use rate of deep soil water by increasing the content of proline in leaves and optimizing the structure of maize root system, so as to improve the long-term drought resistance of crops.

The development of an electromagnetic spectrum monitoring (ESM) system based on a low-earth orbit (LEO) constellation has shown to be an effective method of achieving global ESM and is now a research hotspot in several fields. In the classic LEO-based ESM system, the “on-satellite acquisition and processing” architecture is used in which the satellite gathers and analyzes electromagnetic signal data before transmitting the processed results back to the data center on the ground. Although this framework can reduce the transmission pressure on the satellite-ground link, it yields a limited system performance of the single satellite payload. This paper proposes an intelligent processing framework for the LEO-based ESM system with separate acquisition and processing. In this framework, the satellites serve as forwarding nodes for electromagnetic signal data. The satellites are only responsible for acquiring electromagnetic signal data, which is then processed by a data center on the ground. Unlike the traditional framework, this framework delivers massive amounts of raw electromagnetic data to the ground. To address the problem that the massive data in this framework are difficult to process using traditional technical methods, deep learning is organically integrated with the key technologies of the traditional framework. Deep learning provides a new option for realizing global space–time continuous ESM. The three key technologies involved in the proposed framework are spectrum sensing, blind source separation, and passive positioning based on deep learning, and their research progress in recent years has been sorted out. Compared with ground-based systems, constellation-based systems have the following characteristics: (1) the satellites are far away from the radiation source; (2) the satellites are fast; (3) the satellites show long-distance distribution among them; (4) the topology of the constellation is always in high-speed dynamic change. These characteristics cause a significant divergence between their essential technologies and the research of ground-based systems for these technologies. However, the present efforts relating to essential technologies are based on research conducted on ground-based platforms. There is an issue of applicability to consider when immediately transitioning them to the constellation-based system. Thus, the suitability of each important technology for the migration of constellation-based systems is thoroughly examined. The future trajectory of each major technological breakthrough is then investigated. Finally, recommendations for further studies are made based on the leading technologies of the intelligent processing framework for LEO-based ESM systems.

A radius basis function (RBF) network inverse control method is considered for a class of multi-input multi-output (MIMO) nonaffine nonlinear system. In this method, the nonaffine term which is hard to invert is decomposed into invertible part and un-invertible part, the invertible part is used to approximate the inversion of system and the invert error is approximately canceled by the adaptive signal, the un-invertible part is used to update the RBF network's weights. Using Lyapunov's direct method, it is shown that all the signals of the closed-loop system are uniformly ultimately bounded. Simulation results are provided to show the good tracking performance and effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of fibroblast growth factor receptor 3 (FGFR3) on articular cartilage superficial zone cells (SPZCs). Methods C57 mice were randomly divided into two groups: a sham operated group (sham group) and a group of surgically induced unstable medial meniscus model group (DMM group). The histological morphology of articular cartilage was microscopied by Safranin O/Fast Green-stained in 4 weeks and 8 weeks after surgery. Apoptosis and FGFR3 protein expression were detected by immunohistochemical staining microscopy. Primary SPZCs were separated and randomly divided into control group and Fgfr3 knockdown treatment group. The genes and protein expression related to chondrocyte extra cellular matrix synthesis, degradation and chondrocytehypertrophy were detected by RT-qPCR and Western blot. Results Compared with the sham group, the keen cartilage of mice in DMM group showed a pioneer damage of SPZCs after surgery; Immunohistochemistry results showed an increase in chondrocyte apoptosis and a decrease in expression of MMP-13 and FGFR3(P＜0.05). Primary SPZCs were transfected with small interfering RNA (siRNA) to knockdown Fgfr3; RT-qPCR results showed that the mRNA expression of genes related to the synthesis of cartilage extracellular matrix aggrecan and Col2 was reduced; And the mRNA expression of extracellular matrix degradation-related genes Mmp13 and Adamts5 was increased. The mRNA expression of chondrocyte hypertrophy-related genes Col10 and Mmp13 was increased. Western blot and RT-qPCR results were consistent and the expression l of MMP13 protein was significantly increased, while the expression of collagen Ⅱ and aggrecan protein was significantly decreased (P＜0.05). Conclusions Knockdown of Fgfr3 induces damage to primary SPZCs in mice resulting in early osteoarthritis (OA) development.

A vector semantic computing method study for short sentence based on artificial neural network was proposed. And a semantic computational algorithm for social network texts as well as a discovery algorithm for mergencies was provided with reference to the information provided by the social nodes itself and the semantic of the text. Through the numerization of text, the calculation and comparison of semantic distance, the classification of nodes and the discovery of community can be realized. Then, huge quantities of Sina Weibo contents are collected to verify the model and algorithm put forward. In the end, outlooks for future jobs are provided.

Background and purpose: Pancreatic cancer is a highly malignant disease. Most patients are in advanced stage upon diagnosis. Systemic chemotherapy is an important treatment method, but the chemotherapy drug resistance to tumors brings many problems to clinical treatment. As a commonly used chemotherapy drug, paclitaxel can induce apoptosis in tumor cells. The FBW7 gene is a tumor suppressor gene, and the loss of its function can lead to tumor occurrence and progression. Research has shown that it has the effect of promoting tumor cell apoptosis and inhibiting tumor proliferation. In addition, this gene has been proven to promote apoptosis and ferroptosis, which increase the effect of chemotherapy drugs. Pyroptosis is a programmed cell death mode mediated by gasdermin (GSDM) protein, and this cell death is often accompanied by inflammatory reactions. This study aimed to investigate whether FBW7 gene can promote the anti-tumor effect of paclitaxel by increasing pyroptosis. Methods: A PANC-1 cell line overexpressing FBW7 was constructed using lentivirus transfection. The correlation between FBW7 and GSDME gene expressions was detected by immunohistochemistry in clinical samples, and the expression levels of mRNA and protein were detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) and Western blot. We observed the morphological changes of cells treated with paclitaxel under light microscopy. Cell counting kit-8 (CCK-8) was used to detect the effect of paclitaxel on cell viability, and flow cytometry and lactate dehydrogenase (LDH) release assay were performed to detect the effect of paclitaxel on cell death. Western blot was used to detect caspase-3 and GSDME activation after paclitaxel treatment. Results: RTFQ-PCR and Western blot experiments showed that overexpression of FBW7 gene increased the expression of GSDME. Immunohistochemical staining of pathological sections of clinical patients also showed that the expressions of FBW7 and GSDME genes was positively correlated in vivo. Flow cytometry and LDH release experiments showed that the level of cell death in pancreatic cancer cell line PANC-1 overexpressing FBW7 was significantly increased compared with its empty vector (EV) cells after being treated with paclitaxel. Under light microscopy, it was observed that the number of cells with pyroptosis was significantly higher in PANC-1 cell lines overexpressing FBW7 than in EV cells. The CCK-8 experiment results showed that the cell viability was significantly lower in FBW7 overexpressed cell lines than in EV cells after paclitaxel treatment. Western blot experiment results showed that after pancreatic cancer cell line PANC-1 was treated with paclitaxel, the protein expressions of active-caspase-3 and GSDME-NT in FBW7 overexpression cell lines increased, which proved that they had more obvious activation, indicating that the FBW7 gene can increase the sensitivity of PANC-1 cells to paclitaxel through caspase-3/GSDME signaling pathway induced cell pyroptosis. Conclusion: FBW7 can increase the sensitivity of pancreatic cancer cells to paclitaxel by increasing the expression of GSDME, which is realized through caspase-3/GSDME pathway.

The 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) with anti-inflammatory or pro-inflammatory effects in inflammatory diseases is related to metabolism, as well as in the immune response through regulatpn of glucocorticoid (GC) synthesis. The expression and functional activity of 11β-HSD1 are potentially induced by pro-inflammatory cytokines, this process relies on the NF-κB signaling pathway and is ultimately determined by the utilization levels of HSD11B1 P1 and P2 promoters. In addition, 11β-HSD1 inhibits the proliferation of hepatocellular carcinoma by reducing glucose intake and glycolysis in hepatocellular carcinoma, and thus plays a role in the development of T cells in thymus and the form of angiogenesis by regulating the level of GC. Understanding the molecular regulatory mechanism of 11β-HSD1 in inflammatory diseases, carcinogenesis, development and immune response is conducive to exploring the occurrence and development of related diseases.

Background In China, team-based service delivery model is a major emerging model for contracted family doctor services, but there is a lack of a tool for assessing the overall effectiveness of the family doctorteam. Objective To develop a IMOI model-based system for assessing the effectiveness of family doctor teams in Beijing, aiming at proving a tool for guiding the improvement and continuous development of the family doctor team. Methods The first draft of the Family Doctor Team Effectiveness Evaluation System (FDTEES) was developed using literature review and personal interview. Then the indicators of the system were assessed and revised according to consensuses of our analysis and the results of two rounds of consultations carried out between May and July 2021 using the Delphi technique (one was conducted with 24 experts, and the other with 21 experts) . The weight of the indicators of the system was determined by and analytic network process. Results The response rate of experts in the first, and second round of consultation was 87.5% (21/24) , and 100.0% (21/21) , respectively. The authority coefficients for the consultations ranged from 0.88 to 0.91. Kendall's W for the importance and applicability of the indicators of the FDTEES was 0.138 (P

In aquaculture, feeding intensity can directly reflect the appetite of fish, which is of great significance for guiding feeding and productive practice. However, most of the existing fish feeding intensity evaluation methods have problems of low observation efficiency and low objectivity. In this study, a fish feeding intensity evaluation method based on near-infrared machine vision was proposed to achieve an automatic objective evaluation of fish appetite. Firstly, a near-infrared image acquisition system was built by using near-infrared industrial camera. After a series of image processing steps, the gray level co-occurrence matrix was used to extract the texture feature variable information of the image, including contrast, energy, correlation, inverse gap and entropy. Then the data set were constructed by using these five feature variables as input vectors, and the support vector machine classifier was trained. Among them, the optimal penalty coefficient c and kernel function parameter g were selected by grid search. Finally, the trained images were used to classify the feeding images of fish. And ultimately, the evaluation of fish feeding intensity was realized. The results show that the accuracy of the evaluation could reach 87.78%. In addition, this method does not need to consider the impact of reflections, sprays and other factors on image processing results, so it has strong adaptability and can be used for automatic and objective evaluation of fish appetite, thus provide theoretical basis and methodological support for subsequent feeding decisions.

Aimed to resist the infringement of software services in cloud environment,a software services protection security protocol based on software watermarking in cloud environment was presented.On the consideration of actual needs in cloud computing,the protocol introduced the software watermarking as a service cloud which was responsible for software watermark embedding and extraction.The protocol not only met the basic requirements of software copyright protection,but also gived the detailed strategy to solve the infringement,and hardened the ability of the software watermarking to resist various attacks.The analysis shows that the proposed protocol has higher security.

DOI：10.3969/j.issn.1672-6731.2010.04.008

Objective: To investigate the effects of Sini San prescription(SNS) on the proliferation and apoptosis of HepG2 cells and its molecular mechanism. Methods: The morphological changes of hepatocellular carcinoma HepG2 cells treated by SNS were observed by inverted microscope. MTT assay was used to detect the inhibitory effect of SNS on cell proliferation. Fluorescence staining and flow cytometry were employed to analyze the effect of SNS on apoptosis of HepG2 cells. Rho123 (Rhodamine 123) staining method was performed to detect the changes of mitochondrial membrane potential, and Western blot was used to evaluate the expression of proteins related to apoptosis. Results: The number of hepatocellular carcinoma HepG2 cells were significantly decreased ( P ＜0.01) and cells showed typical apoptotic cell morphology after treated with serum contained SNS. The inhibition rate of HepG2 cells was increased with the increase of concentration of serum contained SNS. The number of cells in G1 phase was significantly increased, while G2 phase was decreased after treated with serum contained SNS( P ＜0.05).The apoptosis rate and mitochondrial membrane potential of HepG2 cells were significantly increased and decreased after treated with serum contained SNS( P ＜0.05). The expression levels of Bax, caspase-3,-9 and cyt-c were significantly increased, while the expression of bcl-2 was decreased in HepG2 cells treated with serum contained SNS( P ＜0.05). Conclusion: Sini San prescription can inhibit the proliferation of HepG2 cells and induce apoptosis by mitochondrial pathway.

Objective: To construct pEGFP-N1-HBsAg-ROP2 recombinant expression plasmid and transfect HEK293T cells for expression, and pay a way for Toxoplasma gondii nucleic acid vaccine development., Methods: According to the HBsAg gene sequence and pcDNA3-p30-ROP2 recombinant plasmid restriction sites, the HBsAg gene was amplified by PCR. The HBsAg gene was cloned into the pcDNA3-p30-ROP2 and instead of p30 gene. The HBsAg-ROP2 fragment was amplified by PCR and digested with Hind Ⅲ and Kpn Ⅰ to clone into the pEGFP-N1 eukaryotic expression vector and construct the recombinant pEGFP-N1-HBsAg-ROP2. The expression vector was transfected into HEK293T cells based on the identification of PCR amplification, restriction endonucleases and sequencing., Results: The PCR product of HBsAg was about 700 bp, which was consistent with the theoretical value. Two bands of about 5.4 kb and 1.9 kb were obtained after double enzyme digestion with pcDNA3HBsAg-ROP2 recombinant plasmid. The recombinant plasmid pEGFP-N1-HBsAg-ROP2 was double-digested to generate an empty vector fragment of about 4.7 kb and a band of about 1.9 kb of HBsAg-ROP2 fragment. The results of sequencing showed that the sequence was 99.84% identical with the published sequence in GenBank. The target plasmid was successfully transfected into HEK293T cells, and the expression was correct, the protein concentration was 3.08 mg/ml., Conclusions: The recombinant plasmid pEGFP-N1-HBsAg-ROP2 is successfully constructed and expressed efficiently.

Objective: To analyze the genotypes and homology of MSP-1 and CSP gene of Plasmodium vivax in Shandong Province, so as to provide the evidence for case traceability., Methods: A total of 12 blood samples were collected from P. vivax -infected cases in Shandong Province in 2011. Parasite genomic DNA was extracted. Primers were designed according to MSP-1 and CSP gene sequences of P. vivax . Then Nested PCR, enzyme digestion, sequencing and sequence alignment, and homologous analysis were performed., Results: The MSP-1 gene of all the 12 samples from P. vivax -infected cases were detected with a 470 bp PCR amplification band, and 350 bp and 120 bp enzyme digestion fragments, which were identified as type Sal-1. An analysis of phylogenetic tree of MSP-1 gene showed that the sequences of 9 indigenous case samples in Shandong Province were located in the same branch, one case sample infected from India was located in the same branch with India strains. All the 12 P. vivax -infected samples covered GDRA (D/A) GQPA sequences in CSP gene, which were identified as type PV-Ⅰ. Of the CSP gene among 12 P. vivax -infected samples, 10 samples of indigenous case in Shandong Province and one sample of the case infected in Guangdong Province were detected with both 560-840 bp and 150-230 bp PCR amplification bands, which were identified as temperate zone family strain of type PV-Ⅰ. However, one sample from the case infected in India was detected only with a 560-840 bp band, which was identified as tropical zone family strain of PV-Ⅰ. An analysis of phylogenetic tree of CSP gene showed that the sequences of 10 samples from the indigenous cases in Shandong Province and one sample from the case infected in Guangdong Province were located in the same branch, one sample from the case infected in India was located in the same branch with India and Indonesia strains., Conclusions: Of all the indigenous isolates in Shandong Province, MSP-1 gene is genotyped type Sal-1, CSP gene is genotyped temperate zone family strain of type PV-Ⅰ, with a high homology found among the indigenous isolates.

Objective: To understand the malaria prevalence and epidemic characteristics in Taixing City from 2005 to 2015, so as to provide the evidences for formulating the prevention and control strategy., Methods: The malaria data of Internet reported cases as well as the reports on epidemiological survey of malaria cases in Taixing City from 2005 to 2015 were collected and analyzed with the methods of descriptive statistics and correlation analysis., Results: A total of 61 malaria cases were reported in Taixing City from 2005 to 2015, including 4 local cases and 57 imported cases. There were 9 cases of vivax malaria, 47 cases of falciparum malaria, 2 cases of malariae malaria, and 3 cases of ovale malaria. There was no seasonality in the occurrence of imported malaria. Though there was no correlation between the times of malaria attack and the duration for going out ( r = 0.154, P = 0.253), there was a negative correlation between the former and the timeliness for visiting a doctor ( r = -0.312, P = 0.014)., Conclusions: The malaria cases are mainly imported ones in Taixing City, and the Plasmodium species of infection are diverse. Imported malaria overseas remains the key point for malaria control in Taixing City.

Objective: To explore the biological function of rhoptry protein 38 (ROP38) of Toxoplasma gondii , and to identify the reactogenicity of the recombinant protein (rROP38)., Methods: The ROP38 was amplified by RT-PCR from T. gondii RH strain, and was cloned into prokaryotic expression vector pET-28a (+). The recombinant plasmid was transformed into E. coli BL21 (DE3) competent cells. Then the rROP38 was analyzed by SDS-PAGE and identified by Western blot., Results: SDS-PAGE showed that rROP38 was efficient expression with a molecular weight of about 43 kD. Western blot showed that rROP38 reacted with antibody of His tag or human positive antibody, which indicated that ROP38 had good reactogenicity and could be a serological diagnostic antigen., Conclusions: The study successfully obtains the rROP38 of T. gondii with good reactogenicity.

Objective: To explore the effects of the transplantation of collagen-chitosan nerve scaffold containing glial cell line-derived neurotrophic factor(GDNF)gene modified schwann cells on the recovery of long-distance sciatic nerve defect., Methods: The rat models of 8 mm long-distance sciatic nerve defect were established and divided into three groups, with 6 rats in each group. In GDNF-Sch group, the defect was repaired by GDNF modified Schwann cells combined with collagen-chitosan nerve scaffold. In Sch group, the defect was repaired by Schwann cells combined with collagen-chitosan nerve scaffold. In the control group, the defect was repaired by autologous nerve graft. Sciatic function index(SFI)was detected 3, 6, and 12 weeks after surgery. After 12 weeks, the tibialis anterior muscle wet weight, electrophysiology, and regenerated nerve morphology were detected., Results: The SFI in the operated side significantly differed among these three groups after 6 and 12 weeks(P<0.05). Along with prolonged treatment, the GDNF-Sch group had similar SFI recovery with the control group but significantly better SFI recovery than Sch group. After 12 weeks, the sensory nerve conduction velocity in the GDNF-Sch and Sch group was not significantly different(P>0.05)but was significantly lower than that in the control group(P<0.05). Both the GDNF-Sch group and Sch group had significantly lower sensory nerve amplitude comparing with the control group(P<0.05), whereas that in the GDNF-Sch group was significantly higher than that in the Sch group(P<0.05). GDNF-Sch group and the control group had significantly higher motor nerve conduction velocity and amplitude than Sch group(P<0.05), while no such statistically significant difference was seen between the two groups(P >0.05). After 12 weeks, the wet weight of the bridging side of the tibial muscle in the control group, Sch group, and GDNF-Sch group was(0.360±0.020), (0.250±0.018), and(0.310±0.025)g, which were significantly lower than the control side [(0.440±0.031), (0.420±0.024), and(0.430±0.027)g, respectively(P<0.05)]. Muscle wet weight in bridge side of GDNF-Sch group and the control group were significantly higher than in Sch group(P<0.05), but it was not significantly different between the GDNF-Sch group and the control group(P>0.05)., Conclusion: Transplantation of collagen-chitosan nerve scaffold containing GDNF gene modified Schwann cells can remarkably facilitate sciatic nerve defect recovery, with a milimar effectiveness as autologous nerve grafting.

Objective: To investigate the effect of aquaporin 4 (AQP4) knock out (KO) on the dysfunction of retinal ganglion cells (RGC) in laser-induced ocular hypertension (OHT) mice., Methods: Experimental study. AQP4 KO mice (n = 18, 36 eyes) and wide type (WT) mice (n = 18, 36 eyes) were used. Unilateral OHT was induced by laser photocoagulation in KO and WT mice after anesthesia. A simultaneous recording of PERG was performed by homemade electrodes. Intraocular pressure (IOP) was measured with an IcareLAB rebound tonometer every day at noon before and after surgery. The data was analysed by ANOVA and t test., Results: In AQP4 KO mice, the mean P50 and P95 amplitudes were significantly (P < 0.01) reduced compared with WT mice [P50: KO: (5.53 ± 1.31) vs WT: (8.14 ± 1.24) µv t = 5.70 and P95: KO: (7.71 ± 1.89) vs WT: (11.30 ± 2.61) µv, t = 3.83, respectively]. The latencies of AQP4 KO mice in both P50 and N95 were shorter than those of WT (t = 5.70 and t = 3.83 respectively, P < 0.01). The mean IOP was significantly (P < 0.05) increased after photocoagulation in AQP4 KO and WT mice compared with pre-photocoagulation [KO post: (14.78 ± 1.80) mm Hg(1 mm Hg = 0.133 kPa) vs KO pre-photocoagulation: (6.61 ± 0.90) mm Hg and WT post: (16.44 ± 1.46) mm Hg vs WT pre-photocoagulation: (7.31 ± 0.98) mm Hg, respectively] with animals under general anesthesia. IOP was lowered mildly but significantly in KO mice when compare with WT mice (t = 3.09, P < 0.05) lasting seven days. IOP was gradually decreased to baseline values at day eight in both of KO and WT mice., Conclusions: Our results indicate that AQP4 null may damage retinal function that can be detected by the measurement of PEGR, a sensitive parameter to reflect the function of RGC.

Objectives: To investigate whether aquaporin 4 (AQP4) gene can affect the activation of glial cells and cause the injury of retina of chronic high intraocular pressure mice models, and to discuss its possible mechanism., Methods: Experimental study. The chronic high intraocular pressure models were established by burning the scleral venous of the right eye, which as the experiment group, and the left eye without any treatment as the control group. The intraocular pressure (IOP) was measured by rebound tonometer. Selected each of the successful model of chronic high intraocular pressure male AQP4 knockout mice (AQP-/-) and their wild-type (WT) male mice 40, divided the two type of mice into five groups after scleral venous burn according to the time of establishing models (24 h, 3 d, 1 w, 2 w, 4 w after scleral venous burn), 8 mouse in each group. And then producing the paraffin sections of mouse eye. Immunohistochemical staining methods was used to observe the expression of the glial fibrillary acid protein (GFAP) in retina glial cells, and observe the expression of the AQP4 in the retina of the WT mouse. Image was acquired under the fluorescence microscopy. The intraocular pressure was analyzed by t-test., Results: After scleral venous burn (24 h, 3 d, 1 w, 2 w, 4 w), there were significant difference (t = 6.66 - 18.08, all P value < 0.05) in the IOP of the AQP4-/- mice (11.30 ± 1.59, 11.20 ± 1.15, 10.60 ± 1.53, 10.75 ± 1.45, 10.45 ± 1.39) and WT mice (11.50 ± 2.56, 11.25 ± 1.65, 10.75 ± 1.33, 10.60 ± 1.33, 10.40 ± 1.19) between the experimental groups and control groups (6.60 ± 0.94, 6.35 ± 0.99, 6.55 ± 0.94, 6.45 ± 0.99, 6.50 ± 0.94 and 6.60 ± 1.05, 6.50 ± 0.89, 6.40 ± 1.09, 6.30 ± 1.13, 6.50 ± 1.05). Since 24 hours after the scleral venous burn, the expression of GAFP of the two type mice began to increase and reached to peak at 1 week after burning. This peak of WT mice was more obvious than that of AQP4-/- mice. The concentration of GAFP began to decrease at 2 weeks after burning and reached to bottom at 4 weeks later. To the WT mice, the expression of AQP4 was remarkable higher in experimental group than that in control group at 1 week after the scleral venous burn. The expression of AQP4 was related to the expression of GAFP in high intraocular pressure of WT mice at 1 w, 2 w, and 4 w after the scleral venous burn., Conclusions: The chronic high intraocular pressure models can be established successfully by burning the scleral venous. AQP4 gene can affect the activation of the glial cells in chronic high intraocular pressure mice and lead to the injury of retina.

Objectives: To determine the prevalence of cancer-related anemia and iron deficiency anemia (IDA) in patients with gastric and colorectal cancer in North of China., Methods: A cross-sectional study of 262 inpatients diagnosed with gastric or colorectal cancer admitted to eight general hospitals in Beijing from August 2009 to December 2009 was performed. The blood samples were took on the day after admission and the seventh day after operation for the tests of hemoglobin, serum iron and ferritin. The morbidity of cancer-related anemia and IDA before and after the surgery was also compared respectively., Results: The preoperative morbidity of cancer-related anemia was 36.6% in 131 patients with gastric cancer, and the morbidity of IDA was 52.1%. The mean age of the anemic patients was higher than that in cases without anemia [(62 ± 11) yrs vs. (57 ± 12) yrs, P < 0.05]; the postoperative morbidity of IDA increased to 72.6% (P < 0.05). In the 131 cases with colorectal cancer, the preoperative incidence of cancer-related anemia and IDA was 37.4% and 61.2%, respectively. About 45% of the cases with anemia had a tumor in the right colon. Postoperative incidence of IDA was significantly higher than that before the surgery (76.7%, P < 0.05). Only 10.3% of the anemic patients were treated with chalybeate therapy before surgical procedures, and the proportion was 22.7% after the operation. More than 50% of anemic patient received blood transfusion., Conclusions: Cancer-related anemia is a common clinical manifestation in patients with gastrointestinal cancer, and anemia occurs more frequently in elder and patients with right colon tumor. The treatment to cancer-related anemia is insufficient and a systematic therapy is needed to be established.

Objective: To investigate the effect of transfection with human soluble vascular endothelial growth factor receptor-1(sFlt-1) gene on cell growth and vascular endothelial growth factor (VEGF) concentration of the culture supernatant in human colon cancer cell line Lovo., Methods: The recombinant plasmid pcDNA3-sFlt-1 containing sFlt-1 gene was transfected into Lovo cells by Lipofectamine 2000, which was identified by RT-PCR and ELISA. The effect of sFlt-1 protein on cell growth and VEGF expression in Lovo cells were investigated by MTT and ELISA., Results: The recombinant plasmid pcDNA3-sFlt-1 was successfully transfected into Lovo cells. The sFlt-1 expression was identified by RT-PCR and ELISA, which inhibited the growth of Lovo cells and reduced the VEGF concentration in the culture supernatant compared with control. The inhibitory rates of proliferation of Lovo cells via MTT assay after 2,14,21 and 28 days were(23.92+/-9.16)%, (13.98+/-10.21)%,(22.54+/-11.92)% and (33.43+/-9.34)% respectively. Compared with the control groups, the differences were significant (P<0.05, P<0.01)., Conclusion: Transfection with sFlt-1 gene into Lovo cells results in the expression of sFlt-1 protein, which possesses high biological activity and inhibits the growth of cancer cells.

Background & Objective: The aim of this study was to investigate the influence of low dosage (131)I-labeled anti-carcinoembryonic antigen (CEA) monoclonal antibody C50 ((131)I-C50) on tumor growth and the therapeutic efficacy of combination of low dosage (131)I-C50 with chemotherapy using 5-fluorouracil (5-FU) on human colorectal cancer xenografts in nude mice., Methods: Human colorectal cancer xenografts with positive CEA expression were established in nude mice with LoVo cell line. 5-FU, 2,775 kBq (131)I-C50, and 5-FU combined with (131)I-C50 were given to nude mice through tail vein to treat xenografts at 9(th) day after implantation of tumor cells. Two of the mice of each group were sacrificed randomly at 7(th) day after the treatment; and cellular ultrastructure of tumor tissues was examined under electron microscope. Pathological changes of tumor tissues were examined under light microscope. Tumor volume, tumor doubling time, and inhibition rate of each group were calculated. Tumor volumes of all groups at 30(th) day after implantation were compared with each other., Results: There was significant difference of tumor volumes at 30(th) day after implantation between each other among control group, chemotherapy group, radioimmunotherapy (RAIT) group, and RAIT+chemotherapy group (P< 0.001). Tumor doubling time of these groups was prolonged and tumor inhibition rates increased successively., Conclusion: Low dosage (131)I-C50 can inhibit tumor growth of human colorectal cancer xenografts in nude mice. Efficacy of tumor growth inhibition can be enhanced by combination of low dosage (131)I-C50 with chemotherapy.