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Objective To investigate the serological changes of blood type and whether hemolytic reactions occurred in a patient with ABw subtype after type A therapeutic plasma exchange(TPE) retrospectively. Methods The ABO blood group identification and genotyping was carried out by microcolumn gel method, microcolumn glass bead method, saline tube method and absorption-elution test, and the laboratory results before and after TPE were analyzed. Results The patient underwent 2 000 mL type A TPE in other hospitals and was transferred to our hospital. On the second day of hospitalization, the test showed consistency between forward and reverse blood typing. On the third day, the detection showed that Bc was ±~1+ , and discrepancy was found between forward and reverse typing. The presence of B antigen was confirmed through tube method and human anti-B absorption-elution test, and was identified as ABw03 by molecular biological method. After type A TPE, Hb and Plt both temporarily decreased, creatinine slightly increased, ALT and AST continued to decrease, while TBIL, DBIL and IBIL showed a temporary decrease followed by an increase, indicating a slight hemolytic reaction. Conclusion Infusion of large volume of plasma may cause hemolytic reactions when ABO subtype patients contain weak A or B antigens.The blood type should be strictly identified according to the ABO blood type forward and reverse typing standards. Additional laboratory tests combined with molecular biology techniques are required for weak agglutination, which can reduce the missed detection of ABO subtypes and reduce the occurrence of transfusion reactions.

In this study, the fresh leaves of Yun kang 10 with one bud and two or three leaves in spring, summer and autumn were used as raw materials. After anaerobic treatment, white tea was processed by the same process. The quality differences of white tea made from raw materials in three seasons after anaerobic treatment were explored by sensory evaluation, physical and chemical composition determination and UHPLC-MS/MS high-throughput detection technology to detect the content of amino acid components. The results showed that there were significant differences in the quality of white tea in the three seasons. The dry tea of white tea in spring was bright green in color. The aroma was strong, lasting, high and intensive, and had a variety of aroma types such as flowery, honey, tip aroma, medicinal and the fragrance of unique process. The taste was strong, fresh, sweet and brisk. The color of tea soup was bright yellow. Infused leaf was soft and bright. The quality of sensory was the best. The contents of amino acids, soluble sugar, caffeine, water extract and flavonoids of white tea were the highest in spring, and the content of tea polyphenols white tea was the highest in summer. A total of twenty amino acids were detected, of which 18 amino acids were significantly different (P

Compared with single-radar systems, spatially separated networked radar usually has better detection performance due to its advantages of spatial and frequency diversities. Most of the current fusion detection methods based on networked radar systems only rely on the echo amplitude information of the target without considering the Doppler information that a coherent radar system can obtain to assist detection of targets. Intuitively, the spatial position and radial velocity of a target observed by different radar devices in the networked radar systems should meet certain physical constraints under which the target and false target can be substantially distinguished. Based on this consideration, fusion detection for the networked radar aided by a Doppler information algorithm is proposed in this paper. First, a set of inequalities is constructed based on the coupling between the observation of the same target’s azimuth and Doppler velocity by multiple radar stations. Then, a two-phase method, an algorithm in operational research, is used to judge whether the inequalities have a feasible solution, based on which a judgment is made on whether the target exists. Finally, some simulations are conducted, which show that the proposed algorithm can effectively improve the detection performance of the networked radar system fusion detection. Additionally, the influence of radar station location and target position on the fusion detection performance of the proposed algorithm is analyzed.

Aiming at the problems of low fault recognition rate， slow convergence speed and difficult parameter selection in the process of fault diagnosis of planetary gearbox based on BP neural network improved by optimization algorithm， a GA-ACO algorithm is proposed to optimize the parameters of neural network. The basic principle and main steps of GA-ACO-BP algorithm are given. At the same time， this method is applied to the fault diagnosis of planetary gearbox. Comparing the performance of ACO-BP neural network algorithm and GA-ACO-BP algorithm， the results show that the convergence speed of ACO Optimized BP neural network is slow and the recognition accuracy is not high， while GA-ACO-BP algorithm can accurately and quickly diagnose and identify the fault of planetary gearbox.

In BeiDou satellite receiver, a fast BeiDou signal acquisition algorithm based on coherent down-sampling was proposed to solve the problem that the large amount of data to be processed by the Fourier transform affects the acquisition speed of satellite signals in the traditional parallel frequency acquisition algorithm. On the basis of FPGA+DSP (field-programmable gate arrays+digital signal processors), the coherent down-sampling module was added to the traditional parallel frequency acquisition algorithm, and after the carrier and pseudo random code were stripped, the sampling frequency was reduced to decrease the number of points to be processed in the Fourier transform, and then a three-dimensional search for the satellite signal was performed. Theoretical analysis shows that the algorithm can reduce the amount of calculation by more than 80%; the experiments on the actual BeiDou signal indicate that the average acquisition time of each star is 9.95 ms, and the memory resource consumption is reduced by 42% compared with the traditional parallel frequency acquisition algorithm. This algorithm can effectively improve the acquisition speed while saving resources, which can provide reference for further improving the acquistition performance of the software receiver.

With the proposal of the carbon peaking and carbon neutrality goals, China's energy structure is gradually shifting from relying on fossil fuels to relying mainly on renewable and clean energy systems. However, clean energy represented by wind and photovoltaic power generation is difficult to operate and consume separately due to output fluctuations and uncertainties. Hydropower stations have good regulation capabilities and can operate in conjunction with wind farms and photovoltaic power stations to alleviate the instability of new energy generation. The southwestern region of China is rich in hydropower resources, and a large number of cascade hydropower stations with regulatory reservoirs have been built. The complementary operation of cascade hydropower with wind power and photovoltaic resources around the watershed, and the full utilization of the regulating capacity of cascade hydropower to bundle and deliver high-quality electricity, is an important means of achieving energy transformation. To quantify the carrying capacity of cascade hydropower stations on wind power and photovoltaic resources, this paper proposes a nested calculation model for medium to long-term optimal scheduling of hydropower to short-term optimal allocation of hydro-wind-photovoltaic power. The short-term scheduling calculation boundary is determined based on the mid-term and long-term optimization scheduling calculation results of hydropower, the scale of wind and photovoltaic power during short-term scheduling is adjusted, and scheduling with the goal of maximizing source load matching, maximizing power generation, and minimizing abandoned power are optimized. Decoupling is carried out by using a hierarchical nested strategy to calculate the scale of wind and photovoltaic resources that can be carried by cascade hydropower stations. Applying this model to the cascade hydropower station group in the Yalong River Basin, the scale of wind and photovoltaic resources carried by the "three reservoirs and seven cascade" cascade hydropower stations in the Yalong River Basin is calculated. The research results show that considering the 2025 planning level year and 5% curtailment rate constraint, the cascade hydropower in the Yalong River basin can carry 1.396 million kW of wind power resources and 10.74 million kW of photovoltaic power resources. The research results have a certain reference value for the optimization of water solar energy complementary power generation systems in the watershed. [ABSTRACT FROM AUTHOR]

ObjectiveTo investigate current status of hospital infection management in psychiatric hospitals in the Ningxia Hui Autonomous Region， so as to provide references for improving the level of hospital infection management of psychiatric hospitals.MethodsIn December 2020， on-site supervision was conducted on hospital infection management in all 9 psychiatric hospitals in the Ningxia Hui Autonomous Region， meantime， the self-compiled questionnaire on hospital infection management status was used for investigation.ResultsAmong the selected hospitals， nine （100.00%） psychiatric hospitals had the main hospital leaders in charge of hospital infection management， five （55.56%） hospitals had established a hospital infection management committee， six （66.67%） hospitals had established an independent hospital infection management department， and one （11.11%） hospital had developed all 13 systems mentioned in the questionnaire related to hospital infection management and job responsibilities. In terms of hospital infection management staff， there were 23 staff members in the nine psychiatric hospitals， including 3 in the specialty （13.04%） and 20 in the part-time setting （86.96%）. The score of the implementation of the basic system of hospital infection management in nine hospitals was （3.28±2.22）.ConclusionThe system specification related to hospital infection management in the Ningxia Hui Autonomous Region psychiatric hospitals needs to be improved and further strengthened， the professionalism of hospital infection management personnel needs to be improved.

The present work is to study the chemical constituents from petroleum ether fraction of Tibetan medicine Swertia chirayita by column chromatography and recrystallization. The structures were identified by physical and chemical properties and spectral data as swerchirin (1), decussatin (2), 1,8-dihydroxy-3,5,7-trimethoxyxanthone (3), 1-hydroxy-3,5,7,8-tetramethoxyxanthone (4), bellidifolin (5), 1-hydroxy-3, 7-dimethoxyxanthone (6), methylswertianin (7), 1-hydroxy-3,5-dimethoxyxanthone (8), erythrodiol (9), oleanolic acid (10), gnetiolactone (11), scopoletin (12), sinapaldehyde (13), syringaldehyde (14), and β-sitosterol (15). Compounds 3, 4, 9, 11-14 were isolated from S. chirayita for the first time. Compounds 9 and 12 were firstly isolated from the genus Swertia. The cytotoxic activities of compounds 1, 2, 5, 7 and 8 against human pancreatic cancer cell lines SW1990 and BxPC-3,and the protective effects of these compounds against hydrogen peroxide (H2O2)-induced oxidative stress in human endothelium-derived EA.hy926 were investigated in vitro. The results showed no obvious effect at the high concentration of 50 μmol•L⁻¹., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)

Objective: To screen mutations of tuberous sclerosis complex (TSC) patients to confirm a clinical diagnosis of TSC, and to perform prenatal diagnosis for families with mutations., Methods: In this study, PCR-denaturing high-performance liquid chromatography(DHPLC), supplemented with sequencing when necessary, was used to screen TSC1 and TSC2 mutations in 21 patients from 19 pedigrees visited author's hospital in the last five years. For novel mutations, one hundred unrelated healthy individuals were screened to exclude the possibility of polymorphism., Results: Seventeen different mutations were found in 21 patients of 19 pedigrees with 13 being novel mutations, including c. 2672delA, c. 2672insA of TSC1 gene and c.4918insCGCC, c.1143delG, Intron27+1 G>A, c.1957-1958delAG, Intron5+1 G>A, c.910insCT, c.2753 C>G, c.4078dupAGCAAGTCCAGCTCCTC, Intron 11 -1 G>A, Intron 14+1 G>A, c.684 C>A of TSC2 gene, indicating a high frequency of de novo mutations in TSC. Three of these mutations were in the TSC1 gene (N762S, c.2672insA and c. 2672delA), while all remaining 14 were in the TSC2 gene. Prenatal diagnosis for TSC was performed for 7 fetuses from these pedigrees. The six fetuses that tested negative for TSC mutations were carried to term and, to date, none of these children has shown symptoms of TSC., Conclusion: Author's data showed that a mutation detection rate of tuberous sclerosis was 89.5%(17/19) among patients in author's hospital. The ratio of TSC2 and TSC1 mutations was about 1:1 in the familial cases, but TSC2 mutation was more common than TSC1 mutation in sporadic cases. Author's data demonstrated that birth of TSC children for those with familial history of TSC could be prevented through prenatal diagnosis.

Objective: To investigate the association of the major histocompatibility complex class I chain-related antigens A (MICA)-129 gene polymorphism and soluble MICA (sMICA) levels with ulcerative colitis (UC) in Hubei Han nationality., Methods: The genetic polymorphism of MICA-129 was examined using a polymerase chain reaction-sequence based test (PCR-SBT) in 256 UC patients and 460 healthy controls. From the above subjects, 80 patients and 90 healthy individuals were randomly selected for determining serum sMICA concentrations by ELISA., Results: The frequencies of variant allele (G) and genotype (GG) in MICA-129 gene were significantly higher in the UC patients than in the controls (76.8% vs 72.2%, P = 0.060; 55.9% vs 46.3%, P = 0.016). Serum sMICA levels were significantly elevated in the patients compared to the controls [(576.47 ± 279.02) ng/L vs (182.17 ± 73.11) ng/L, P < 0.001]. In addition, the sMICA levels were higher in the patients carrying MICA-129 GG genotypes than in those carrying (GA + AA) genotypes [(638.87 ± 347.15) ng/L vs (507.51 ± 152.87) ng/L, P = 0.035]., Conclusions: The genetic polymorphism of MICA-129 and sMICA levels are correlated with the UC patients in Hubei Han nationality. Our findings demonstrate that MICA-129 gene may contribute to the pathogenesis of UC.

Objectives: The present study aimed to investigate the associations between genetic polymorphism of methylenetetrahydrofolate reductase (MTHFR) G1793A, plasma homocysteine (Hcy) levels, vitamin status and ulcerative colitis (UC) in a cohort of patients in Hubei Han nationality., Methods: Two hundred and ninety-nine UC patients and 764 age- and sex-matched healthy controls were recruited in this study. Polymorphism of MTHFR G1793A was examined using a PCR-RELP method. Plasma levels of Hcy, folate and vitamin B12 were determined by enzymatic cycling assay and corpuscle immune chemiluminescence assay, respectively., Results: Both variant allele and genotype frequencies in MTHFR G1793A gene were significantly higher in the UC patients compared to the controls (22.24% vs 14.20%, P < 0.001; 42.81% vs 26.97%, P<0.001, respectively). Plasma Hcy levels were increased in UC patients compared to the controls [(20.67 ± 6.42) mmol/L vs (13.21±5.11) mmol/L, P<0.001] while folate and vitamin B12 concentrations were significantly decreased [(11.37±6.34) nmol/L vs (14.89±7.21) nmol/L, P<0.001; (324.15±127.53) pmol/L vs (421.54±128.45) pmol/L, P<0.001, respectively]. Furthermore, hyperhomocysteinaemia (HHcy) and folate deficiency were also more prevalent in the UC patients (32.44% vs 25.78%, P=0.029; 23.41% vs 17.01%, P=0.016, respectively)., Conclusions: Genetic polymorphism of MTHFR G1793A was strongly associated with UC. HHcy, folate deficiency and low vitamin B12 concentration were common phenomena in the UC patients of Hubei Han nationality. Our findings demonstrate that the genes related to Hcy metabolism may play an important role in the pathogenesis of UC.

Objective: To detect gene mutation in the patients with autosomal dominant polycystic kidney disease (PKD)., Methods: Polymerase chain reaction (PCR)-denaturing high-performance liquid chromatography (DHPLC) analyses were performed in 3o single copy region of PKD 1 gene (PKD1). DNA sequencing were carried out on PCR products with abnormal peak shape afterwards., Results: A new nonsense mutation (C11901A in exon 42 of PKD1 was identified to cause serine in position 3897 turning to a stop codon. A missense mutation, C10737T, was detected in exon 35 which caused threonine in position 3509 turn to methionine. Two kinds of samesense mutation, G11824A and C11860T in exon 42, were found in normal control., Conclusion: PKD1 mutation were detected successfully by PCR-DHPLC. A new nonsense mutation, a missense mutation and two polymorphisms are identified in this study.

Soil and rape samples around Tongling mining area were collected, and their copper (Cu) contents were investigated. The results showed that the upland soil developed on the slope deposit around the mining area as well as the paddy soil distributed in lower reaches was heavily polluted by Cu, while the fluvo-aquic soil further from the mining area was less contaminated. Though the Cu content in paddy soil and upland soil was nearly the same, its bioavailability was higher in paddy soil, due to the Cu pollution of irrigated water. There was a significant correlation between available and total Cu in these three types of soil. The activation rate of soil Cu (percentage of available Cu in total Cu) was 15.0% on average, which was positively correlated with soil total Cu and organic matter while negatively correlated with soil pH and Mn. The average Cu content in rape seed and stalk was 4.0 and 5.8 mg x kg(-1), respectively. The rape Cu content increased obviously with increasing soil available Cu content when the soil available Cu content was relatively low, but the Cu absorption and accumulation by rape decreased gradually when the soil available copper content was higher than 30 mg x kg(-1).

Objective: To explore the influence factors on amplification of single cell duplex-nested PCR., Methods: The mutational loci region CD41-42 and IVS-II 654 of beta-globin gene were amplified by duplex-nested PCR with different combination of primers concentration, different Taq DNA polymerases, different neutralization buffers and with or without predenaturation at 98 degrees C before the PCR amplification in single lymphocyte or single blastomere, thus, to investigate the influence of these factors on the amplification efficiency of PCR., Results: TaKaRa EX Taq was the most efficient Taq DNA polymerase among different Taq DNA polymerases; primer pair R1+F1 at final concentration of 0.25 micromol/L and R2+F2 at 0.3 micromol/L were the most efficient ones in amplification among different combinations of primers concentrations; the amplification efficiency in neutralization buffer-1 (200 mmol/L Tricine) was obviously higher than that of neutralization buffer-2 (900 mmol/L Tris-HCl, pH 8.3/300 mmol/L KCl/200 mmol/L HCl)(P<0.05); there were no remarkable differences of the amplification efficiency while using whether predenaturation at 98 degrees C before the single cell PCR amplification or not (P>0.05)., Conclusion: There were remarkable differences of the amplification efficiency of single cell duplex-nested PCR while using different combination of primers concentrations, different Taq DNA polymerases, different neutralization buffers. However, predenaturation at 98 degrees C before the single cell PCR amplification could not improve the PCR amplification efficiency.

Objective: To improve the accuracy and the diagnostic rate of gene diagnosis and prenatal gene diagnosis for hemophilia A (HA) families., Methods: Linkage analysis was performed by using St14(DXS52) VNTR polymorphism and intron 13 (CA)n repeat polymorphism of the factor VIII gene among HA families for indirect diagnosis., Results: The diagnostic rates using linkage analysis based upon one of the above mentioned two polymorphic loci among 9 HA families were 66.7% and 66.7%, respectively. The diagnostic rate rose to 88.9% by using a combination of the two polymorphic loci. Prenatal gene diagnoses were performed for 4 HA families. A wrong prenatal diagnosis which may happen when linkage analysis was performed by using only St14 VNTR was monitored., Conclusion: The rapid and accurate gene diagnosis and prenatal gene diagnosis could be performed by a combination of the two polymorphic loci for about 90% HA families.

Objective: To investigate the molecular mechanism of a Chinese patient with 46, XY sex reversal., Methods: DNA fragments of the SRY gene from the typical XY female sex reversal patient and her father were amplified by polymerase chain reaction (PCR). The amplified PCR fragments were cloned into the pUCm-T vector, and direct sequencing was carried out on an ABI 377-3 automated DNA sequencer to detect the mutation. PCR-restriction enzyme digestion was applied to detect the results of DNA sequencing., Results: A novel mutation of the SRY gene was identified in the XY sex reversal patient of this study. A T is replaced by an A in codon 129 at position +387, resulting in the replacement of the polar amino acid tyrosine (TAT) by the stop code (TAA) in the HMG-box, whereas her father was proved to have the wild-type sequence. Because the mutation introduced an enzyme site of MaeIII, the PCR-restrict enzyme digestion showed that there were three bands (131 bp,231 bp and 247 bp) in the patient, whereas two bands (131 bp and 478 bp) in normal man. It verified the results of sequencing analysis. The results after searching the Human Gene Mutation Database showed that this mutation was not described before and should be a new mutation., Conclusion: The novel mutation in SRY gene has provided valuable information for the understanding of molecular mechanism of the patient with 46,XY female sex reversal.

Objective: To research on the reliability of diagnosing achondroplasia (ACH) on single cell level and to provide a basis for preimplantation genetic diagnosis(PGD)., Methods: The high-frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested-PCR with single lymphocyte and single blastomere. The products of PCR were digested by restriction enzyme Bfm I, then the digested products were detected by 10% polyacrylamida gel electrophoresis(PAGE)., Results: The amplification success rate, allele dropout rate and correct diagnosis rate of single lymphocyte's PCR were 90.4%, 8.2% and 91.8%,respectively. The amplification success rate of single blastomere was 75.4%., Conclusion: The diagnosis of ACH by single cell nested-PCR is comparatively stable and reliable.