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Copyright of Journal of Prevention & Treatment For Stomatological Diseases is the property of Journal of Prevention & Treatment For Stomatological Diseases Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB/Akt) signaling pathway plays an important role in various biological processes, including cell growth, proliferation, differentiation, and apoptosis. The PI3K/Akt-related signaling pathways have been extensively studied, and they regulate neural cells to exert different or similar functions. This study reviews the research progress on PI3K/Akt-related signaling pathways in apoptosis, autophagy, oxidative stress, neuroinflammation, and other pathophysiological mechanisms in neural cells. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of calcium ions on the expression of kallikrein-4 (KLK4) and cell growth of ameloblast, and to provide an experimental basis for calcium ion promoting normal mineralization of enamel. Methods ALC cells were treated with 0, 2.0, 2.5, 3.0, and 3.5 mmol/L CaCl2 for 24 and 48 h. KLK4 expression was analyzed by qRT-PCR and Western blot analysis. The viability of ALC cells was determined by using CCK-8. AnnexinV-FITC/PI dual staining combined with flow cytometry and Hoechst 33342 staining were used to detect the ALC cell cycle and cell apoptosis. The protein expression level of glucose-regulated protein 78 (GRP78) was measured by Western blot analysis. Results After 24 h of treatment with 2.5, 3.0, and 3.5 mmol/L CaCl2, the expression of KLK4 mRNA was increased (P2, the expression of KLK4 protein was increased (P2, the expression of KLK4 mRNA and protein was increased (P2 (P2. Hoechst 33342 staining results showed that 3.0 mmol/L and 3.5 mmol/L CaCl2 may promote apoptosis in ALC cells. Flow cytometry showed that the proportion of G2/M phase cells and the apoptosis rate increased after 3.5 mmol /L CaCl2 treatment for 24 h (P2 groups. After 24 h of treatment with 3.0 mmol/L and 3.5 mmol/L CaCl2, the expression of GRP78 protein was reduced (P2, the expression of GRP78 protein was reduced (P

BACKGROUND: silencing information regulatory 1 (SIRT1) regulates the function of related proteins in chondrocytes in a deacetylated manner and participates in chondrocyte proliferation and differentiation, thereby promoting cartilage defect repair. OBJECTIVE: To screen for signaling pathways with unclear action status after SIRT1 gene knockdown in chondrocytes, as well as diseases or functions that produce changes using high-throughput technology. METHODS: ATDC5 chondrocytes from mice in logarithmic growth phase were divided into two groups: the cells were transfected with SIRT1 gene knockdown negative control lentivirus in control group and SIRT1 gene knockdown lentivirus in experimental group. GeneChip® Mouse Genome 430 2.0 Array was used to detect the mRNA expression at 72 hours after transfection. Applied bioinformatics technology was also used to screen for unclear activation or inhibition signaling pathways and their related factors. Moreover, enrichment of disease or function modules was analyzed. RESULTS AND CONCLUSION: After knocking down the SIRT1 gene, there were 245 signaling pathways with unclear activation or inhibition status in the mouse ATDC5 chondrocytes. According to the ranking of -Log (P-value), we reported the factors in the top 20 signaling pathways with unclear activation or inhibition status, including IGFBP4, TGFBR1, CTGF, COL4A5, LHX2, IL1RL1, and KLF6. According to the ranking of -Log (P-value), there were significant changes in 14 disease or function modules, including cellular growth and proliferation, organism survival, cell death and survival. According to the number of differentially expressed genes, there were significant changes in three disease or function modules, including organismal injury and abnormalities, cancer, and cell death and survival. According to the comprehensive ranking of -Log (P-value) and the number of differentially expressed genes, the disease or function module related to intrinsic immune response was significantly activated. [ABSTRACT FROM AUTHOR]

The experiment aimed to optimize the fermentation protease activity of the engineered Bacillus subtilis strain WB800N/pHT43-npr-PrsA, providing technical support for domestic feed protease enterprises. The fermentation process was monitored for glucose content and specific consumption rate to determine the dynamic impact of carbon and nitrogen sources on the glucose consumption of this strain. The components of the fermentation medium were optimized through single-factor and response surface experiments. The results showed that optimizing the fermentation medium with the OD600 nm value of the cell body from 0 to 10 hours as a parameter can promote cell growth and achieve a higher cell quantity. After 10 hours, supplementing with 5 g/L yeast extract and 70 g/L peptone could extend the stationary phase to achieve the maximum specific enzyme activity. The optimized fermentation medium conditions were: 60 g/L yeast extract, 30 g/L peptone, 10 g/L racemic glucose, and 3 g/L MgSO4. At 8 h of fermentation, adding 0.5‰ IPTG and 2‰ protease inhibitor, the final fermentation protease activity reached 1 156 U/mL. The study indicates that delayed stable period can increase the peptone content in the medium, which is beneficial for improving the specific glucose consumption rate of the cells. Peptone promotes the adaptation of the cells to the surrounding environment. Maintaining a low glucose concentration during the fermentation process can effectively avoid the formation of acetic acid, promote cell growth, and enhance enzyme production efficiency, keeping the cell consumption of the substrate at its most vigorous state. [ABSTRACT FROM AUTHOR]

Objective: To analyze and explore the expression of TRIM29 in osteosarcoma and its potential molecular mechanism. E3 ubiquitination ligase TRIM29 is closely related to the malignant growth of tumors. Methods: The expression of TRIM29 gene in osteosarcoma was analyzed by Western blot, IHC and qRT-PCR, CCK8 and EDU were used to detect the value-added level of osteosarcoma cells after downregulation of TRIM29. Further, glucose uptake, lactate production and G6P were used to detect the changes of glycolysis level and analyze the underlying mechanism. A subcutaneous tumor model of BACB/C nude mice was established to observe the effect of downregulation of TRIM29 on the growth of transplanted tumors. Results: qRT-PCR results confirmed that the mRNA expression level of TRIM29 in osteosarcoma tissues was significantly higher than that in normal tissues. Similarly, Western blot and IHC results also confirmed that the protein level of TRIM29 in osteosarcoma tissues was significantly higher. After downregulating the expression of TRIM29 in osteosarcoma cells, CCK-8 and EDU experiments both showed that its value-added ability was weakened, on the contrary, its apoptosis rate was increased. In the G1 phase of cells, its lactate production, glucose consumption, G6P were also correspondingly weakened, and the glycolytic joint rate limiting enzymes HKII, PFKFB3, PKM, LDHAA, GLUT1 and other proteins were also correspondingly reduced. Similarly, in vivo experiments also showed that after downregulating the expression of TRIM29, the weight and growth rate were reduced accordingly. Conclusion: TRIM29 is highly expressed in osteosarcoma cells. After inhibiting the expression of TRIM29 in osteosarcoma cells, its cell proliferation ability and glycolysis level are decreased. TRIM29 inhibits the growth of osteosarcoma cells by restraining the glycolysis level of cells [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chinese Institute of Food Science & Technology is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of South China University of Technology (Natural Science Edition) is the property of South China University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To improve the growth and sporulation efficiency of Bacillus subtilis, the effect of malate on the cell growth and metabolism of B. subtilis DF was investigated in a 30 L bioreactor. At 22 h of fermentation, a biomass of 3.68 × 1010 CFU/mL was obtained by malate addition to a final concentration of 1.5% during 16–27 h after the pH reached 8.0 at 16 h, which was 71.9% higher than that of the control group. Meanwhile, the number of spores was 3.63 × 1010 CFU/mL, which was 92.1% higher than that of the control group. Differential transcriptomic analysis showed that malate increased the metabolic efficiency of the gluconeogenesis and pentose phosphate pathways with 1.38 to 2.51-fold up-regulation of the zwf, pckA and fbaA genes, respectively and the metabolic efficiency of the tricarboxylic acid (TCA) cycle with 3.10-fold up-regulation of the citZ gene, reduced cellular oxidative stress with a 72.9% down-regulation of the poxL gene, a 3.88-fold up-regulation of the trxA gene, and thus a 45.9%–51.3% reduction in H2O2 concentration during 19–24 h, and enhanced the phosphorylation efficiency of Spo0A with down-regulation of the kipL, rapAD and abrB genes by 57.5%–75.9%, and up-regulation of the clpX, phrCF, spo0B and sigA genes by 1.73–13.76 folds, significantly improving the growth and sporulation efficiency of B. subtilis DF. These findings provide theoretical supports for the industrial application of B. subtilis.

Objective: To investigate the effects of miR-150 on expression of PD-1 in peripheral T cells and lung tumor growth in mice. Methods: miR-150 gene knockout (miR-150KO) and wild-type normal control (WT) mice were selected, number changes of peripheral T cells,expression of activated phenotype molecule CD69, and immune checkpoint molecule PD-1 were detected by flow cytometry. Lewis lung cancer cell line (LLC) was used to prepare transplanted tumor models in miR-150KO and WT mice by subcutaneous injection, and effect of miR-150 on growth of lung tumor was observed. Anti-PD-1 antibody was used to intraperitoneally inject miR-150KO and WT tumor-bearing mice, and its effect on tumor growth inhibition was observed. Results: Compared with WT mice, proportion and number of CD4+T cells in lymph nodes and spleen of miR-150KO mice were significantly increased, while proportion and number of CD4+T and CD8+T cells in peripheral blood were significantly decreased. miR-150 knockout resulted in a significant increase in proportion of CD69+ cells in CD4 T and CD8 T cells of lymph nodes and peripheral blood, as well as a significant increase in proportion of PD-1+ cells in CD4 T and CD8 T cells of peripheral blood. Compared with WT tumor-bearing mice, tumor growth was significantly accelerated in miR-150KO tumor-bearing mice, and proportion of PD-1+ cells in CD4 T and CD8 T cells in peripheral blood and lymph nodes of miR-150KO tumor-bearing mice was also significantly increased. Anti-PD-1 antibody treatment significantly inhibited tumor growth of miR-150KO tumor-bearing mice. Conclusion: miR-150 knockout promotes the growth of transplanted lung tumor in mice by increasing expression of PD-1 in T cells, but also enhances the immunosuppressive effect of anti-PD-1 antibody on tumor. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Mucor circinelloides has the ability to use a wide range of carbon sources to accumulate lipids. The different carbon sources have significantly influenced the total lipids through different metabolic pathways in oleaginous organisms. In the present study, the effects of exogenous oil (soybean oil) at different concentrations as an alternative carbon source on its fungal growth and lipid accumulation were investigated without changing fermentation culture conditions and the original C/N ratio, while using 100% glucose as a control. Total lipids and their fatty acid composition were determined by gas chromatography (GC). Moreover, the expression levels of desaturases genes involved in fatty acid biosynthesis were investigated in the studied strains using real-time fluorescence quantitative PCR. The results showed that a concentration of 35 g/L of glucose mixed with 24.3 g/L of soybean oil were the optimal carbon sources with constant carbon to nitrogen ratio. The biomass, lipid content and lipid yield of M. circinelloides grown in this medium increased rapidly in the early stage of fermentation, and the growth trend gradually slowed down in the late stage of fermentation, decreasing slightly after 72 h of fermentation. It was found that the highest biomass, total lipids, and lipid yields were significantly increased at 19.60 g/L, 42.62%, and 8.35 g/L (33.15%, 23.8%, and 65.10%), respectively. In contrast, the control group (those cultured on glucose as a single carbon source) was only 14.72 g/L, 34.41%, and 5.06 g/L, respectively. The critical step was in which α-linolenic acid (ALA) was stored from the exogenous oil in fungal cells supplying enough soybean oil with mixed substrates for fatty acids synthesis. The overexpression of the △12-desaturase gene in WJ11 significantly (P

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the regulation mechanism of HOXC8 and PDX1 in the growth and epithelial mesenchymal transition (EMT) of non-small lung cancer (NSCLC) cells. Methods: RNA-sequence analyses, Chromatin immunoprecipitation (ChIP) and Real-time PCR assays were performed to identify HOXC8-targeted genes; Western blot, CCK-8, colony formation and bioinformatics analyses were carried out to examine the roles of PDX1 gene in NSCLC. Results: HOXC8 was shown to bind to the promoter of the PDX1 gene and act as a transcription factor to activate the expression of PDX1. PDX1 expression promoted the growth and EMT of NSCLC cells, while silencing PDX1 significantly inhibited the growth and EMT of NSCLC, and induced cell apoptosis. PDX1 expression was significantly higher in NSCLC specimens than that of normal tissues by analyzing public tumor databases, and growth and EMT of NSCLC, and induced cell apoptosis. Analyses of public tumor databases indicated that PDX1 expression was significantly up-regulated in NSCLC specimens in comparison with normal tissues, and high PDX1 expression was significantly correlated with poor survival of lung cancer patients. Conclusion: This study showed that HOXC8-PDX1 axis played an important role in the progression of NSCLC and would be a new target for NSCLC treatment. [ABSTRACT FROM AUTHOR]

BACKGROUND: Polyetheretherketone materials have the advantages of stable physical, chemical and mechanical properties, radiation transparency and elastic modulus similar to human bones, but there is a biological inertia problem, which limits its clinical application. OBJECTIVE: To evaluate the biocompatibility of polyetheretherketone/hydroxyapatite composites prepared by fused deposition molding 3D printing. METHODS: Polyetheretherketone and polyetheretherketone/hydroxyapatite composite materials (hydroxyapatite mass fraction 10%) were prepared by fused deposition molding 3D printing technology, which were denoted as polyetheretherketone group (control group) and polyetheretherketone/hydroxyapatite group (experimental group). Passage 3 mouse preosteoblasts (MC3T3-E1) were co-cultured with the extracts of the two groups of materials, separately. Cells cultured alone were used as positive controls. The survival and proliferation of MC3T3-E1 were detected by Live/Dead fluorescence staining and CCK-8 assay. The adhesion growth and proliferation of MC3T3-E1 on the surface of the materials were observed by scanning electron microscopy. RESULTS AND CONCLUSION: (1) Live/Dead fluorescence staining exhibited that the MC3T3-E1 cells in two kinds of extracts grew well and presented good viability. The cell survival rate was higher than 90%. The two materials had no obvious cytotoxicity. The cells proliferated well with the extension of culture time. (2) The results of CCK-8 assay showed that the cytotoxicity of the two kinds of materials was qualified. The proliferation absorbance value of cells in the experimental group was higher than that of positive control group and control group at 3, 5 and 7 days of culture (P < 0.05). (3) Scanning electron microscope observation showed the good adhesion and cell spreading. Matrix secretion of MC3T3-E1 cells was numerous in the experimental group at 1 day of inoculation. The number of cells adhering to the surface of the two groups of materials increased, and more matrixes were secreted at the same time, among which the number of cells adhering to the surface of the experimental group and the secretion of matrix were more at 3 days of inoculation. The overlapping growth of cells appeared on the surface of the two groups of materials, and the cells in the experimental group were more efficient in spreading growth and secreting matrix at 7 days of inoculation. (4) These findings verify that the polyetheretherketone/hydroxyapatite composite prepared by 3D printing has good biocompatibility. [ABSTRACT FROM AUTHOR]

Ectoine can protect biological macromolecules against adverse environmental damage such as osmotic pressure change, high temperature, freezing, drying and radiation, and has no adverse effect on cell growth. However, due to the high price and unclear protection mechanism, the application scope is limit- ed. Therefore, the possible protection mechanism and research methods of ectoine are reviewed, with emphasis on the protection effect of ectoine under adverse environmental conditions. Finally, the necessity of this research is summarized and its future research direction is prospected. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of circ_0016788 targeting miR-338-3p on the proliferation, apoptosis, migration and invasion of prostate cancer cells. Methods circ_0016788 and miR-338-3p expressions were detected by RT-qPCR in prostate cancer tissues.si-circ_0016788, si-NC, miR-NC, miR-338-3p mimic, si-circ_0016788+anti-miR-NC, si-circ_0016788+ anti-miR-338-3p were transfected into DU145 cells respectively. CCK-8, Transwell method and flow cytometry were applied to detect DU145 cells growth, migration, invasion and apoptosis rate. The interaction between circ_0016788 and miR-338-3p was confirmed by dual luciferase reporter assay. Results circ_0016788 was highly expressed（P＜0.05） while miR-338-3p was lowly expressed（P＜0.05） in prostate cancer tissues. After inhibiting the expression of circ_0016788, the cell inhibition rate, apoptosis rate and miR-338-3p expression level were increased（P＜0.05）, and the numbers of migration and invasion were decreased（P＜0.05）. After overexpression of circ_0016788, the cell inhibition rate and apoptosis rate were decreased（P＜0.05）, and the numbers of migration and invasion were increased（P＜0.05）. miR-338-3p was a direct target gene of circ_0016788. Inhibition of miR-338-3p expression significantly attenuated the effects of circ_0016788 low expression on DU145 cells inhibition rate, migration and invasion number, and cell apoptosis rate（P＜0.05）. Conclusion Inhibition of circ_0016788 can induce prostate cancer cell apoptosis and inhibit proliferation, migration and invasion by promoting miR-338-3pexpression. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Hydrobiology is the property of Editorial Department of Journal of Hydrobiology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the regulatory mechanism of circular RNA MRPS35 (circMRPS35) on the proliferation, apoptosis, migration and invasion of gastric cancer (GC) cells. Methods: Human GC cell lines (HGC-27, MGC-803, MKN45 and AGS) and normal gastric epithelial GES-1 cells were cultured in vitro, real-time quantitative PCR（RT-qPCR） was used to detect the m RNA expressions of circMRPS35, mi R-130a-3p and zinc and ring finger 3 (ZNRF3). MGC-803 cells were also taken and grouped into control group, pc-NC group, pc-circMRPS35 group, pc-circMRPS35 combined with mi R-NC group, and pc-circMRPS35 combined with mi R-130a-3p group. Plasmid transfection using Lipofectamine 3000. RT-qPCR was used to detect the m RNA expression of circMRPS35, mi R-130a-3p and ZNRF3. Western blot was used to detect ZNRF3 protein expression, CCK-8 method and flow cytometry were used to detect cell proliferation and apoptosis, scratch assay and Transwell chamber assay were used to detect cell migration and invasion abilities, nude mouse xenograft experiments were performed to investigate the effect of circMRPS35 on the growth of GC cells in vivo. Dual-luciferase reporter genes were implemented to detect the target relationship of mi R-130a-3p with circMRPS35 or ZNRF3. Results: In GC cell lines, the m RNA expression of circMRPS35 and ZNRF3 was low, and the expression of mi R-130a-3p was high (all P<0.05). Overexpression of circMRPS35 was able to reduce the expression of mi R-130a-3p, up-regulate the m RNA and protein levels of ZNRF3,inhibit cell proliferation, migration and invasion, and promote cell apoptosis (all P<0.05). The inhibitory effect of circMRPS35 overexpression on malignant behavior of GC cells and tumor growth in nude mice can be reversed by mi R-130a-3p mimic (P<0.05). The results of dual luciferase experiment showed that overexpression of mi R-130a-3p was able to reduce the luciferase activities of circMRPS35-WT and ZNRF3-WT (P<0.05). Conclusion: CircMRPS35 may inhibit the proliferation, migration and invasion of GC cells and promote apoptosis through the mi R-130a-3p/ZNRF3 axis. [ABSTRACT FROM AUTHOR]

In order to construct a dual-functional B. subtilis with probiotic function and L-lysine production, B. subtilis ACCC11025 was systematically modified. These results indicated that the strain with replacement of thrD,zwf and gnd from B. subtilis by lysC311,zwf234 and gnd361 from C. glutamicum (i. e., B. subtilis XH4) was beneficial to L-lysine production, and the yield of L-lysine was (20. 3 ±1.9) g/L. In addition, the strain with replacement of hom from B. subtilis by hom59 from C. glutamicum (i.e., B. subtilis XH5) produced (23. 2±1. 7) g/L of L-lysine without the decrease of cell growth. In addition, the yield of by-products in B. subtilis XH5 was significantly decreased. Moreover, the DapDH from C. glutamicum was introduced into the B. subtilis XH5 (i. e, B. subtilis XH6), resulting in the increase of L-lysine production because of the redirection of the carbon flux in DAP pathway. The resulted recombinant strain B. subtilis XH6 produced (25. 6±2. 3) g/L of L-lysine. [ABSTRACT FROM AUTHOR]

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To preliminarily reveal the mechanism of miR-155 affects the sensitivity of colorectal cancer cells to 5-FU chemotherapy by targeting the expression level of TP53INP1. Method The human colorectal cancer cell line HCT116 was cultured, and the total RNA was extracted, then the reverse transcription system was constructed with miR-155 reverse transcription specific primer for PCR amplification, the expression of miR-155 in HCT116/Fu and HCT116 was detected by qRT PCR; Logarithmic growth cells were transfected with miR-155 mimics, miR-155 inhibitor and miR-155 negative control respectively, the effect of miR-155 on 5-FU drug sensitivity was detected by CCK-8 method, and the dual-luciferase reporter assay was used to verify the relationship between miR-155 and TP53INP1, the effect of miR-155 on the expression of TP53INP1 was detected by Western blot. Results The expression of miR-155 in HCT116/Fu cells was 7.25 times higher than that in HCT116 cells; At the same 5-FU Concentration, the rate of cell growth inhibition of HCT116+negative control was higher than that of HCT116+mimics, and the half inhibitory concentration was significantly lower than that of HCT116+mimics (P<0.05); TP53INP1 was the target gene of miR-155, which could significantly reduce the luciferase activity of wild-type TP53INP1 3'-UTR; After transfection of miR-155 mimics, the relative expression of TP53INP1 decreased significantly, and after transfection of miR-155 inhibitor, the relative expression of TP53INP1 increased significantly (all P<0.05). Conclusion The increased level of miR-155 decreased the sensitivity of HCT116 cells to 5-FU, miR-155 may affect the sensitivity of colorectal cancer cells to 5-FU by targeted regulating the expression level of TP53INP1. [ABSTRACT FROM AUTHOR]

Objective To explore the effect and possible mechanism of phospholipase D2(PLD2) gene expression on the growth of colorectal cancer cells from in vivo experiments.Methods The nude mice model of colorectal cancer (SW480 and SW620) was established.When tumor size reached 50-70 mm³,adenovirus carrying human PLD2-siRNA,or no virus was injected into the tumor in multiple directions.The cell lines were divided into the PLD2 interference group,mock-vehicle group and normal group.The real-time quantitative PCR and western blotting were used to detect the mRNA and protein expression level of the PLD2 in tumors.According to the maximum transverse diameter (L) and maximum longitudinal diameter (S) of the tumor were measured during the experiment,and the tumor growth curve was calculated after calculating the tumor approximate volume.The tumor mass of each cell group was compared.Western blotting was used to detect the expression levels of the c-Myc and Bcl-2 proteins in tumor.Results The PLD2-siRNA led to the decrease in mRNA and protein expression of the PLD2 in tumors (P <0.001).And the interference with reduced PLD2 gene expression led to the growth of colorectal cancer cells inhibit (P <0.001).At the same time,the expression of c-Myc protein was decreased (P <0.001),while the expression of Bcl-2 was increased (P <0.001).Conclusion The interfering PLD2 gene expression may lead to the inhibition of the growth of colorectal cancer cells,which may be closely related to the decrease of the c-Myc expression and increase of Bcl-2 expression.It's suggested that the PLD2 may be an effective target for the colorectal cancer treatment. [ABSTRACT FROM AUTHOR]

The production of Green Fluorescent Protein in recombinant BGC823 cells was used as a model to investigate how to optimize sorting parameters and conditions with FACS Aria Ⅲ. Cells were sorted with nozzles with different orifice diameters, cell samples at different cell concentrations were sorted, and five sorting modes were employed, to compare the proportions of single cells and the clone formation rates in different wells for 96-well plate sorting; and compare the purities of sorted cells and cell growth rates for tube sorting, respectively. The use of the 100 μm nozzle and cell concentration at 5×106cells/mL is recommended for 96-well plate sorting of the cell types similar to BGC-823, to increase the proportion of wells which contain single cells and the clone formation rate. Taking into account both the purity of the sorted cells and time consumption for sorting, the use of the 100 μm nozzle, cell concentrations at 5×106cells/mL and 4-way purity mode is recommended for tube sorting. [ABSTRACT FROM AUTHOR]

BACKGROUND: Cell immortalization is a characteristic of cells acquiring the ability to continue to proliferate. The large T antigen gene can improve the efficiency of cell immortalization, and is widely used in experiments, attracting more and more attention from researchers. OBJECTIVE: To establish an immortalized cell line of human bone marrow mesenchymal stem cells and lay the foundation for the clinical application of human bone marrow mesenchymal stem cells. METHODS: The recombinant plasmid vector containing the SV40 large T antigen gene was used to transfect human bone marrow mesenchymal stem cells, which were successively subcultured and identified by methods, such as morphology, cell proliferation ability, cell surface markers, and multidirectional differentiation potential. RESULTS AND CONCLUSION: (1) The immortalized cell line constructed by this method is an adherent growth of spindle cells with specific surface markers of human bone marrow mesenchymal stem cells. (2) The 50th generation of human bone marrow mesenchymal stem cells can still express SV40 large T antigen gene after transfection. (3) The proliferation ability of the immortalized cell line is better than that of untransfected human bone marrow mesenchymal stem cells. (4) The immortalized cell line has the potential to differentiate into osteogenic, adipogenic, and chondrogenic cells. (5) This shows that transfection of the SV40 large T antigen gene does not affect the biological characteristics and differentiation ability of human bone marrow mesenchymal stem cells. This method can be used to establish immortalized human bone marrow mesenchymal stem cells. [ABSTRACT FROM AUTHOR]

The PI3K/Akt/mTOR signaling pathway are closely related to the regulation of cell growth, which can be a pivotal part that participates in production and development of bovine adipocytes, bovine mammary epithelial cells and bovine oocytes, can also be a participant, indirectly regulating inflammatory factors, metabolic processes, et al. The paper reviews the components, mechanism and effect on growth of different cells of PI3K/Akt/mTOR signal pathway, to provide reference for the follow-up study on how livestock cell growth. [ABSTRACT FROM AUTHOR]

Copyright of Asian Journals of Ecotoxicology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Light is the key factor affecting photoautotrophic growth of microalgae. The huge advantages of light quality supplied by light-emitting diodes (LEDs) (LQSLs) in promoting microalgal growth and target chemicals accumulation have been gaining great attention from science and the relevant industries. Moreover, the microalgal cultivation process based on LQSLs is an emerging technology for high-efficient production of microalgae-based chemicals. This review aims to provide an overview of the absorption spectra and photoreceptors in microalgae, the effects of different LQSLs on microalgae growth and target chemicals accumulation, and the cultivation strategies for high-efficient production of target chemicals by harnessing LQSLs. The path forward for further economical and high-efficient production of microalgal target chemicals using LQSLs with respect to 4 different opportunities is also discussed, including novel LEDs development to enhance the conversion efficiencies of electricity-to-light and light-to-biomass, high- cell-density cultivation of microalgae using LQSLs technology for carbon neutrality, next-generation photobioreactors design based on internal LEDs illumination for industrial application, and employing different LQSLs as signals for promoting the production of light-dependent chemicals in microalgae under heterotrophic/mixotrophic cultivation. This will in turn accelerate the development of microalgae industry. [ABSTRACT FROM AUTHOR]

Copyright of Journal of East China University of Science & Technology is the property of Journal of East China University of Science & Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Different varieties have different resistances. In order to further explore the resistance differences in different varieties of pitaya and to provide a reference for further study on breeding of pitaya resistance, we used Illumina HiSeq 2000 sequence platform to sequence the transcriptomes of ‘Putongbairou'(BR)and ‘Ecuador Yellow'(EY). Functional classification and enrichment analysis of differentially expressed genes(DEGs)were performed by reference to GO Ontology, KEGG and others databases. The results were as follows:(1)There were 14 248 DEGs between BR and EY, of which 5 446 genes were up-regulated and 8 802 genes were down-regulated.(2)GO functional analysis showed that these DEGs were mainly involved in enzyme catalytic activity, cell components, metabolic processes, etc. Among them, there were 349 differential genes involved in oxidoreductase activity.(3)KEGG pathway analysis showed that most of the DEGs were enriched in metabolism, biosynthesis, etc., and 12 key genes such as CYP86 and CER1 involving in cutin, suberine and wax biosynthesis. We found that the expressions of DEGs involved in oxidoreductase activity were higher in BR than those in EY, which significantly enriched, indicating that may be significant differences in growth and cell metabolism between BR and EY. DEGs involved in the cutin, suberine and wax biosynthesis were up-regulated in BR, and such genes had higher expressions in BR, and were significantly enriched, which suggest that BR may be superior drought and disease resistance than EY. [ABSTRACT FROM AUTHOR]

Copyright of Asian Journals of Ecotoxicology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Harbin University of Science & Technology is the property of Journal of Harbin University of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Based on the finite element method, the effects of four different methods of lumbar interbody fusion ( ALIF, PLIF, TLIF and DLIF) on the lumbar fusion segments and adjacent segments under the condition of whole-body vibration were compared. Some important indexes related to complications such as stress distribution, stress peak and stress amplitude were introduced and compared. The results showed that ALIF can provide a more stable environment for the adjacent segments in the whole-body vibration state among the four different methods, and this method has better anti-vibration performance. Compared with the other methods, DLIF can reduce the risk of cage subsidence and failure. Besides, DLIF can provide a more stable and suitable environment for the growth of vertebral cells in the state of whole-body vibration, which is more conducive to the success of vertebral fusion. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Donghua University (Natural Science Edition) is the property of Journal of Donghua University (Natural Science) Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[ Objective] A good suspension cell culture system of Thesium chinese Turcz. is established, so as to lay a foundation for the subsequent use of genetic engineering to transform the metabolic pathway of Thesium chinese Turcz., obtain new varieties of high-order metabolites, and realize the substitution of wild resources. [ Method] On the basis of exploring the law of growth and nutrient consumption of Thesium chinese Turcz. suspension cells, the initial input of liquid nitrogen and phosphorus and the ratio of ammonium nitrogen to nitrate nitrogen (NH+4/ NO-3 ) were optimized. [Result] In the 30 days culture process, the growth curve of suspension cells showed an obvious delay period ( 0 - 6 days), logarithmic growth period ( 7 - 15 days) ), stable growth period ( 16 - 24 days) and decay period ( after 24 days) . The results showed that the dry matter mass of suspension cells reached the maximum when the initial total nitrogen concentration was 70 mmol/L and the phosphate concentration was 1. 3 mmol/L for 28 days, and it was unhealthy for the growth of cells when the nitrogen concentration and phosphate were higher and lower. On this basis, the demand of suspension cultured cells for nitrate nitrogen and ammonium nitrogen were explored. The results showed that the suspension cells during the culture process consumed more nitrate nitrogen than ammonium nitrogen, while the utilization and consumption of ammonium nitrogen precedes that of nitrate nitrogen and too high concentration of ammonium nitrogen would inhibit cell growth. It was most suitable for cell growth when NH+4 / NO+3- was 20/50. [ Conclusion] The growth curve of Thesium chinese Turcz. suspension cells shows ' S ' as taking MS as the basic medium, the concentration of nitrogen is 70 mmol/L, the concentration of phosphate is 1. 3 mmol/L, and the concentration of NH+4/NO-3 is 20/50, which is more conducive to the suspension culture of Thesium chinese cells. Under these conditions, the consumption of total sugar, sucrose, reducing sugar, nitrate nitrogen and inorganic phosphorus are faster in the logarithmic growth period, and the consumption of ammonium nitrogen is faster than nitrate nitrogen in the early growth period. [ABSTRACT FROM AUTHOR]

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Previous studies have found that cinnamaldehyde can promote the biological properties of osteoblasts in high glucose environment. OBJECTIVE: To observe the effect of cinnamaldehyde on proliferation and differentiation of osteoblasts under high glucose with electrospinning membrane as carrier. METHODS: The polycaprolactone membranes were prepared by electrospinning. MC3T3-E1 cells in logarithmic growth phase were divided into four groups: blank control group was added with high glucose medium. Polycaprolactone group was added with polycaprolactone membrane scaffold and high glucose medium. Drug group was added with high glucose medium containing cinnamaldehyde. Combined group was added with polycaprolactone membrane and high glucose medium containing cinnamaldehyde. CCK-8 method was used to detect cell proliferation and alkaline phosphatase kit was used to detect alkaline phosphatase activity at 1, 4 and 7 days of culture. RT-qPCR was used to detect the expression of osteocalcin mRNA at 7 days. RESULTS AND CONCLUSION: (1) CCK-8 assay showed that the cell proliferation of polycaprolactone group, drug group and combined group was faster than that of blank control group at each time point (P < 0.05). Cell proliferation of combined group was faster than that of polycaprolactone group and drug group at 4 and 7 days (P < 0.05), and cell proliferation of drug group was faster than that of polycaprolactone group at 4 and 7 days (P < 0.05). (2) The alkaline phosphatase activity of polycaprolactone group, drug group, and combined group was higher than that of blank control group at 4 and 7 days (P < 0.05). The alkaline phosphatase activity of combined group was higher than that of polycaprolactone group and drug group at 4 and 7 days (P < 0.05). The alkaline phosphatase activity of drug group was higher than that of polycaprolactone group at 7 days (P < 0.05). (3) The expression of osteocalcin mRNA in the four groups from high to low was the combined group, drug group, polycaprolactone group, and blank control group; there were significant differences between the two groups (P < 0.05). (4) Results suggest that polycaprolactone electrospinning membrane and cinnamaldehyde can promote the proliferation and differentiation of osteoblasts in high glucose environment. [ABSTRACT FROM AUTHOR]

Copyright of Acta Materiae Compositae Sinica is the property of Acta Materiea Compositae Sinica Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To study the combined toxicity of pesticides mixtures in agricultural products, five pesticides with wide application and broad spectrum, including difenoconazole, cypermethrin, dimethomorph, cyhalothrin, acetamiprid, and relevant binary and ternary combinations with high exposure levels in lettuce were selected. Cell Counting Assay Kit-8 method was used to determine the proliferation inhibition toxicity of single pesticide and mixtures on HepG2 hepatocytes. The combined effects were assessed by three prediction models, concentration addition, independent action and combination index, based on the concentration-response curve of individual pesticides. Then the induction effects of two ternary combinations on cell apoptosis were analyzed by High Content Screening. The results showed that the effects of different pesticide combinations on the cell growth inhibitory rate of HepG2 cells were different, all of which had obvious concentration-response relationship. Among them, difenoconazole had a strong inhibition of proliferation toxicity, and the median effective concentration was 24.72 μmol/L. The order of toxicity for the other individual pesticides was ranked as dimethomorph > cyhalothrin > acetamiprid > cypermethrin. The combined toxicity of the pesticide mixtures was determined by the combination index. The results showed that with the increase of pesticide concentrations, the combined effects of all binary combinations changed from antagonism to synergy. While in ternary mixtures, including dimethomorph + difenoconazole + cypermethrin and dimethomorph + cypermethrin + acetamiprid, the combined effects changed from synergy to antagonism when the growth inhibition effect were 60% and 18%, respectively. In addition, the dose reduction index was used to quantify the synergistic effect between pesticide mixtures, indicating that cypermethrin played a greater role in the synergistic effect of the above two ternary combinations. In the measurement of apoptosis toxicity, the apoptotic rate of HepG2 cells significantly increased in the groups treated by individual pesticides in a concentration-dependent manner. The apoptotic rate induced by the ternary combination of dimethomorph + cypermethrin + acetamiprid was significantly increased in concentration between of 20.02-80.10 μmol/L. This work confirmed that the toxicities of proliferation inhibition and apoptosis induction of different pesticides mixtures on HepG2 cells both showed obvious concentration-response relationship, which could provide a scientific basis for toxicity evaluation and risk monitoring of pesticides mixtures. The combination index model as an effective ecotoxicological risk assessment tool can quantitatively describe the interactions of pesticide mixtures under different components and concentrations, which can predict the combined toxicity of pesticide mixtures more accurately compared with the concentration addition model and the independent action model. [ABSTRACT FROM AUTHOR]

Objective To investigate the regulatory effect of radix hedysari polysaccharide on the cell apoptosis, growth and motility of ovarian cancer SKOV3 cells. Methods The ovarian cancer SKOV3 cells were treated with 0, 3, 6, 12.5, 25, 50, 100, 200, 400, 800 and 1 600 mg/L radix hedysari polysaccharide. CCK8 method was used to screen the appropriate dose. RT-PCR was used to detect tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) mRNA expression levels. Flow cytometry was used to detect cell apoptosis. The clone formation test was used to detect cell growth. Transwell method was used to detect cell invasion, and Western blot assay was used to detect the expression levels of vascular endothelial growth factor (VEGF), epithelial cadherin (E-cadherin) and fibronectin (FN). Results The optimal doses of radix hedysari polysaccharide were 0, 50, 100 and 200 mg/L for the subsequent experiments. Compared with the 0 mg/L radix hedysari polysaccharide group, the expression levels of TNF-α, iNOS and IL-6 mRNA were significantly down-regulated in ovarian cancer SKOV3 cells of 100 mg/L and 200 mg/L radix hedysari polysaccharide groups (P＜0.05), the cell apoptotic rate was significantly increased (P＜0.05), clone formation rate and the number of invasive cells were significantly decreased (P＜0.05), E-cadherin protein expression was up-regulated (P＜0.05), and the protein expression levels of VEGF and FN were significantly down-regulated (P＜0.05). Conclusion Radix hedysari polysaccharide promotes the apoptosis of ovarian cancer SKOV3 cells and inhibits cell growth and movement. [ABSTRACT FROM AUTHOR]

BACKGROUND: Extracellular matrix can provide a living environment for different types of cells in the body and affect their biological activities, playing an important role in maintaining cell homeostasis and tissue development. OBJECTIVE: To review the research progress of extracellular matrix and its application in tissue engineering regeneration. METHODS: Databases of PubMed and CNKI were retrieved with the key words of “extracellular matrix, tissue engineering, regeneration” in English and Chinese search from 2010 to 2020, respectively. RESULTS AND CONCLUSION: The extracellular matrix contains a variety of components, and its extraction methods are diverse. It has many functions, such as guiding cells, transmitting signals, diagnosing and treating diseases, regenerating and repairing. As a precise and orderly network structure, extracellular matrix has unique advantages in tissue regeneration and repair. Extracellular matrix provides a microenvironment closest to the growth of cells in vivo, and it is rich in various active molecules, which can provide the basis for cell activity, and has a good application prospect. The forms of extracellular matrix existing in tissue regeneration are varied, and it has good biodegradability and low immunogenicity, which can also improve the inflammatory response to a certain extent, can support tissue regeneration and guide tissue reconstruction effectively. With the continuous in-depth study to extracellular matrix, its application in tissue engineering will be better developed, and it will also bring emerging technologies and treatment methods for regenerative medicine and tissue engineering in the future. However, as for the problems faced by extracellular matrix in tissue repair applications, further research and discussion are needed. [ABSTRACT FROM AUTHOR]