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Aim: To investigate the metabolic pathways of dipfluzine in rats., Methods: After an oral dose of dipfluzine (80 mg x kg(-1)) to rats, urine was collected for 12 h. The metabolites of dipfluzine in urine were chromatographed and identified by LC/DAD/MS methods., Results: In the rat urine, there were 1-(4-fluorophenyl)-4-piperazinylbutanone and its glucuronide, 4-hydroxybenzophenone and its glucuronide, 4-fluoro-gamma-hydroxybenzenebutanoic acid and its glucuronide and sulfate, diphenylmethanol and its glucuronide, dipfluzine, and benzophenone., Conclusion: In rats, dipfluzine was mainly metabolized in the pathways of N-desalkylation at 1- and 4-positions of piperazine ring. Some of metabolites were further conjugated with glucuronic acid and/or sulfuric acid.

Objective: Screen seventeen benzodizepines and their metabolitesin urine by GC/ECD and GC/MS., Methods: They were GC (GC/ECD, GC/MS) assay of benzodizepines and GC (GC/ECD, GC/MS) assay of benzophenones of acid-hydrolytic products of 1,4-benzodizepines., Results: The methods were simple and sensitive. The recoverys were 60% to 90% of most benzodizepines, linear calibration curves were 20 ng/ml-200 ng/ml (r > 0.99), and detection limits were 0.5 ng/ml-10 ng/ml., Conclusion: They methods were evaluated with human urine samples.