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Objective To obtain the monitoring measurement range of quality indicators of red blood cells, plasma and derivatives and leukocyte-reduced apheresis platelets provided by blood stations in Hebei province, explore the distribution of monitoring values and the change of monitoring level, so as to further strengthen the homogenization construction of quality control laboratories in blood stations in Hebei. Methods In 2023, the sampling data of 12 blood stations in Hebei from 2015 to 2022 were collected, scatter plots were made and the range markers were set, and the "mean±SD" line was taken as the upper limit and lower limit of the measurement range. In 2024, the monitoring values in 2023 were added, and the changes of two measurement ranges were compared to analyze the stability and overall level. Results Comparison of the measurement range from 2015 to 2022 and the measurement range from 2015 to 2023 showed that the standard deviation of the content of deleukocyte suspension of red blood cells-hemoglobin, washed erythrocyte-hemoglobin, washed erythrocyte-supernatant protein, cryoprecipitate coagulation factor-FⅧ, fresh frozen plasma-FⅧ, leukocyte-reduced apheresis platelets-leukocyte residue and leukocyte-reduced apheresis platelet-red blood cell concentration decreased from 8.132 to 7.993, 6.252 to 6.104, 0.273 to 0.267, 57.506 to 56.276, 0.920 to 0.892, 0.653 to 0.644 and 2.653 to 2.603, respectively.The narrowing of the standard deviation range of the above items led to more concentrated monitoring values and reduced dispersion. Comparison of the measurement range from 2015 to 2022 and the measurement range from 2015 to 2023 showed that the mean value of leukocyte residue of the deleukocyte suspension of red blood cells, hemoglobin content of the wash erythrocyte, protein content of supernatant of the wash erythrocyte, hemolysis rate of the wash erythrocyte, FⅧ content of the cryoprecipitate coagulation factor, plasma protein content of the fresh frozen plasma, FⅧ content of the fresh frozen plasma, platelet content of the leukocyte-reduced apheresis platelets changed from 0.362 to 0.476, 44.915 to 44.861, 0.280 to 0.283, 0.137 to 0.142, 133.989 to 133.271, 60.262 to 60.208, 1.301 to 1.277 and 3.036 to 3.033, respectively, and was closer to the national standard line, which reflects an increase in the number of unqualified monitoring values or values close to the national standard line in 2023. The long-term qualified rate of coagulation items was low, and no improvement has been observed. The stability of biochemical items has been enhanced but overall deviation has occurred, with the average value close to the national standard line. The possibility of subsequent testing failure has increased. The counting items showed no obvious common characteristics. Conclusion The use of "mean±SD" in the analysis can visually display the distribution of monitoring values of different items in Hebei, forming an indicator measurement range covering the past nine years. It shows the characteristics of each item, and provides reference for subsequent quality control laboratory data analysis of each blood stations to takes active measures to improve the monitoring level.

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forensic Science & Technology is the property of Institute of Forensic Science, Ministry of Public Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: To probe into the effect of different preservation conditions on monitoring the blood concentration of cyclosporine in whole blood sample. METHODS: A total of 80 discarded samples for routine monitoring of blood concentration were collected from this hospital, each sample was mixed and evenly divided into 3 parts, which were preserved at room temperature (20-25 ℃), refrigerated (2-8 ℃) and frozen (-35--30 ℃) away from light for 7 days. Chemiluminescence microparticle immunoassay was adopted to detect the blood concentration of cyclosporine under different preservation conditions. RESULTS: The concentrations of cyclosporine were (213. 0± 128. 3) ng/ mL at room temperature and (207. 4 ± 121. 7) ng/ mL at frozen condition for 7 days, which had no statistically significant differences compared with the blood concentrations of (212. 0±123. 5) ng/ mL measured on the sampled day (P =0. 792, P =0. 188); while the concentration of cyclosporine at refrigerated condition for 7 days was (198. 4±113. 8) ng/ mL, which was 6. 6% lower than that measured on the sampled day, with statistically significant difference (P =0. 002). CONCLUSIONS: The blood concentration of cyclosporine is generally decreased after 7 days of refrigeration, while 7 days of preservation at room temperature or frozen have no effect on the blood concentration of cyclosporine. If the cyclosporine sample cannot be detected in time on the day of sampling, it can be preserved at room temperature or frozen away from light, and the determination should be completed within 7 days. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forensic Science & Technology is the property of Institute of Forensic Science, Ministry of Public Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The newly established accurate analysis module for detecting circulating tumor cells (CTCs) (Metafer 5) was used in this study. In order to achieve accurate analysis by Metafer 5, the digital module was optimized. Thirty-three malignant tumors patients were enrolled. Meanwhile, in order to acquire the best application parameters by Metafer 5, we screened the best fluorescent dyes by comparing the characteristics of 4', 6-diamidino-2-phenylindole (DAPI), Rhodamine, fluorescein isothiocyanate (FITC) and TexRed, assessed accuracy and efficiency of CTCs scanning among 10×PA, 10×PN, and 20×PA objective lens and optimized the exposure time of each fluorescent channel. Additionally, CTCs identification was evaluated by both manual examination and Metafer 5 system. According to the data, the results showed that scanning time of CTCs by two objective lens was (114.70±22.08) and (116.50±47.38) min, respectively, and the 10×PA objective lens presented higher accuracy and efficiency. When the exposure time of DAPI, EpCAM, CD45 and CEP8 was adjusted to 0.003, 0.800, 0.040 and 0.068 s, respectively, the clearest and best quality fluorescent images were presented. At the same time, comparing to the manual work, based on the optimal parameters of Metafer 5, not only the time cost was 73.40 min less (P<0.05), but also the accuracy reached 100.00%, which was 8.83% higher than manual work. Above all, the optimized Metafer 5 system could detect CTCs automatically, accurately and quickly in blood sample, which could be used for auxiliary interpretation of CTCs, and could have a future application in clinical practice. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Donghua University (Natural Science Edition) is the property of Journal of Donghua University (Natural Science) Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: The degradation time of biomaterials is crucial in the clinical application of guided bone regeneration surgery. OBJECTIVE: To systematically compare the difference in degradation performance of platelet-rich fibrin membrane and concentrated growth factor membrane in vitro, and analyze the effects of different machines of the same type and different blood collection tubes on the degradation performance of concentrated growth factor membrane. METHODS: Platelet-rich fibrin membranes and concentrated growth factor membranes were prepared with a Multi-CGF-3 centrifuge, and soaked in saliva to observe the changes in membrane quality and area. Two centrifuges of the same type were used to prepare concentrated growth factor membranes, which were then soaked in saliva to observe changes in membrane quality and area. Two types of vacuum blood collection tubes (BD tube and Kangjie tube) were used to prepare concentrated growth factor membranes, and the membranes were soaked in saliva to observe changes in membrane quality and area. RESULTS AND CONCLUSION: (1) The in vitro degradation performance of the platelet-rich fibrin membrane and concentrated growth factor membrane was different. The initial mass of the concentrated growth factor membrane was larger and its degradation time in vitro was longer. (2) The initial mass of the concentrated growth factor membranes prepared by different machines of the same type had no significant difference. The smaller the initial area-to-mass ratio, the slower the degradation rate of the concentrated growth factor membrane. (3) Compared with the Kangjie tube, the BD tube had a better layering effect on blood samples after centrifugation; however, the degradation time and overall degradation performance of concentrated growth factor membranes prepared by the two test tubes were similar. (4) The results show that, as the latest generation of autologous platelet-concentrated products, the concentrated growth factor membrane exhibits better degradation performance than the platelet-rich fibrin membrane. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] To accelerate the breeding of high yield musk deer population, the present paper analyzed the correlation between the polymorphism sites of FSHβ gene and LHβ gene and musk production performance to find out the marker sites of forest musk deer musk production performance from the genetic level. [Method] Using the new generation sequencing technology,100 blood samples of forest musk deer were scanned by DNA mixed pool sequencing method to focus on the gene region or the 2 kb of upstream and downstream regions of 2 candidate genes. 20 candidate SNP sites of 74 forest musk deer were analyzed by mass spectrometry. In combination with the actual production,the association analysis was carried out with these SNPs by using musk production data. [Result] Two SNP sites in FSHβ and one SNP site in LHβ were highly correlated with forest musk performance (P< O. 05), all of which were located in the non-coding region of the gene. These results suggested that these mutations might be related to CIS regulation. [Conclusion] The discovery of 3 mutation sites can be used as molecular markers for artificial selection of forest musk deer and provide auxiliary way for the breeding and selection of high-yield musk deer population. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to study the effects of different breeding systems on emotional factors (5-hydroxytryptamine 5 -HT, beta-endorphin β-EP, Cortisol, etc.) and the mRNA expression of antidepressant factor Pll gene. [Method] Total 60 60-day-old Suzhong pigs (BW =28.4 ± 0. 30 kg) were randomly assigned to two treatments and raised in the concrete floor house (CFH) and deep-litter house (DLH) system. Each treatment had 3 replicates and each replicate for CFH and DLH had 10 pigs. The trial lasted 90 days (45 days, the early fatting stage; 90 days, later fatting stage). On the 45th and 90th days, blood samples were collected for testing emotional indexes of pigs. On the 90 days,hypothalamus tissue was gained for testing the mRNA expression of p11 gene using RT-PCR. [Result] (i) In saliva,the concentrations of 5-HT and Cortisol in DIE group were significantly higher than that in CFH group (P < O. 05),while there were no significant difference in the concentrations of β-EP and acetylcholine (Ach) (P >0. 05). (ii) In early fatting stage on the 45 days, the concentration of semm β-EP, Cortisol and zinc (Zn) in DLH group were significantly higher than that in CFH group (P< 0. 05). Serum 5-HT and Ach had a tendency to increase (P.0.065, P = 0. 062),while the concentration of magnesium (Mg) was not influenced (P >0. 05). In the later stage of fattening (on the 90 day),the concentrations of semm β-EP,cortisol and 5-HT in DLH group were significantly higher than that in CFH group (P< 0. 05), the concentration of semm ach was significantly lower in DLH group (P< 0.05). The concentration of serum Zn and Mg was not significantly difference (P >0.05) . (iii) RT-PCR results showed that the mRNA expression of P11 gene in the hypothalamusin of DLH group was increased with an increasing tendency (P = 0.062). [Conclusion] The deep-litter house system can improve the secretion of emotional material and also promote the secretion of anti-depression P11 protein in pigs' brain, which may reduce pigs' physiological stress and improve the breeding welfare. The pigs are happy and healthy to grow in the deep-litter house system. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Shanghai Physical Education Institute / Shanghai Tiyu Xueyuan Xuebao is the property of Shanghai Physical Education Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The present study was conducted to investigate the effects of acute cold stress on physiological and biochemical Parameters of mice and the intervention function of Mahuang decoction. [ Method] A total of 36 mice were randomly divided into control group, model group and Mahuang decoction group, 12 mice in each group. Before the cold treatment, the mice of Mahuang decoction group were intragastrically administered with Mahuang decoction, the mice of the control and model groups were given distilled water. At the end of the experiment, blood and liver samples were collected to detect blood physiological, biochemical indexes and liver tissue antioxidant indexes. [Result] Compared with control group, the blood RBC,HCT and HGB counts of model group mice increased (P >0.05) ,WBC count decreased significantly (P <0.01) . The serum AST, Glu and CK levels increased very significantly (P < 0. 01) ,and urea level increased significantly (P <0. 05) . The MDA level in liver tissues increased significantly (P < 0. 01) ,and SOD activity decreased significantly (P < 0. 01 ) . Compared with model group, the blood RBC and WBC of Mahuang decoction group mice increased (P > 0. 05). The serum urea, Glu and CK levels decreased very significantly (P < 0. 01) , AST and ALT levels decreased (P >0. 05) . The SOD activity and GSII level in liver tissues increased significantly (P < 0. 05) , MDA level decreased significantly (P < 0. 01 ). [Conclusion] Mahuang decoction has protective effect for acute cold stress in mice. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the effect of a new PHD inhibitor Roxadustat on renal ischemia-reperfusion injury in mice and its possible mechanism .Method: Male C57BL/6 mice were randomly divided into four groups: sham operation group (sham), injury group (IR), injury + low dose group (IR+Rox10 mg/kg) and injury + high dose group (IR+Rox25 mg/kg). Except for the sham operation group, the other groups were given drugs at 1 h, 6 h and 12 h before modeling, and blood samples were taken at 6 h, 12 h, 24 h and 48 h after modeling to detect serum creatinine (Scr) and urea nitrogen (BUN), 1 d, 2 d and 5 d for pathological examination. In addition, the hypoxia model was established by HK-2 cells, and the changes of cell viability and apoptosis, as well as the expression of apoptosis pathway protein and HIF-1 α were measured. Result: Compared with sham group and IR group, the levels of Scr and BUN in high dose group were significantly lower than those in low dose group, and the levels of Scr and BUN in high dose group were significantly lower than those in low dose group, and the morphological damage was lighter and the apoptosis was significantly reduced in the treatment group. Cytological experiments showed that Roxadustat could increase the vitality of HK-2 cells under hypoxia, reduce apoptosis, inhibit the increase of Bax induced by hypoxia and increase the expression of Bcl-2, while HIF-1 α inhibitor 2 MeOE2, It can eliminate the inhibitory effect of Roxadustat on apoptosis. Conclusion: Roxadustat can protect the kidney from ischemia-reperfusion injury in mice through up-regulating the expression of HIF-1 α, inhibiting the expression of apoptosis pathway-related proteins and reducing apoptosis. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of high altitude and low oxygen cold environment on the levels of renal function in healthy Population. Methods: 30 healthy people living in Lanzhou area who radical into the plate(3300 meters above sea level and-4 degrees Celsius daily mean temperature) were selected and compared. All subjects were entered the plateau before and after 24 h, 72 h, 7 d,14 d and 28 d. Urinary IL-18, urine NAGL, urinary KIM-1 and Scr were measured. Results: A total of 28 people completed all six urine and blood samples were collected and tested, of which two women removed due to a collection of points in a menstrual period. Including17 males and 11 females, the average age was 26.8 ± 3.2 years(male 28.3 ± 4.2 years, 24.8 ± 2.6 years). The levels of urinary IL-18 were 3.62±0.32 ng/L, 11.20±0.65 ng/L(P<0.001), 6.32±0.46 ng/L(P<0.001), 4.36±0.68 ng/L(P<0.05), 3.58±0.71 ng/L, 3.32±0.46 ng/L at before and after entering the plateau 24 h, 72 h, 7 d, 14 d and 28 d. The levels of urinary NAGL were 0.126±0.20 μg/L, 0.513±0.003 μg/L(P<0.001), 0.116±0.006 μg/L, 0.009±0.001 μg/L, 0.121±0.010 μg/L, 0.632±0.009 μg/L at before and after entering the plateau 24 h, 72 h, 7 d, 14 d and 28 d. The levels of urinary KIM-1 were 2.61±0.22 ng/L, 18.20±0.69 ng/L(P<0.001), 6.32±0.46 ng/L(P<0.001), 6.36±0.68 ng/L(P<0.001), 2.58±0.31 ng/L, 2.32±0.26 ng/L at before and after entering the plateau 24 h, 72 h, 7 d, 14 d and28 d, The levels of serum creatinine(Scr) were 61.0±9.16 μmol/L, 58.5±8.13 μmol/L, 80.3±10.38 μmol/L(P<0.05), 76.5±12.04μmol/L(P<0.05), 62.6 ±10.14 μmol/L 62.3 ±8.18 μmol/L before and at 24 h, 72 h, 7 d, 14 d and 28 d after entering the plateau.Conclusions: The renal function of healthy Population who rapidly entered the high altitude cold environment was severely damaged, and the urine IL-18, KIM-1, NAGL levels changed early and were more sensitivity than Serum creatinine. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Real-time fluorescence quantitative PCR(RT-PCR) was used to detect the placenta-specific gene 4 (PLAC4) gene in the peripheral plasma of pregnant women at different gestational weeks in order to find reliable biomarkers for prenatal diagnosis of Down's syndrome and provide a new breakthrough for non-invasive prenatal diagnosis. Methods: According to the standard, 5 healthy non-pregnant women, 60 normal pregnant women (including 20 early pregnancy, 20 medium-term pregnancy and 20 late pregnancy), 8 down syndrome screening high-risk pregnant women and 5 postpartum 24 h women were randomly selected. Then 78 peripheral blood samples were collected from them. Detection of PLAC4 gene content in samples by RT-PCR and relative quantitative analysis. Results: None of healthy non-pregnant women and postpartum 24 h women have detected the PLAC4 mRNA gene, Normal pregnancy at different gestational weeks has detected the PLAC4 mRNA gene. Early pregnancy as a control, PLAC4 mRNA gene content of the medium-term pregnancy was 1.99 times of early pregnancy and late pregnancy PLAC4 mRNA content was 3.73 times of early pregnancy. Down syndrome screening high-risk pregnant women has detected the PLAC4 mRNA gene and content was 6.36 times of early pregnancy. Conclusions: PLAC4 mRNA gene is expected to become the reliable biological indicator of prenatal diagnosis of Down's syndrome. [ABSTRACT FROM AUTHOR]

Objective: This study aims to investigate the predictive value of circulating tumor cells(CTCs) for chemotherapy response in gastric cancer patients. Methods: Totally 112 gastric cancer patients receiving operation were involved in this retrospective study. Blood samples from these patients were obtained and CTCs were counted at baseline. In 36 stage Ⅲ gastric cancer patients of them, CTCs were longitudinally enumerated during the treatments. The relation of CTCs value and clinical data concerning patients was evaluated. Results: At baseline analysis of all patients, CTCs positivity(>2) was detected in 44 patients(39.29%), and it was correlated with the stage classification and lymph node metastasis(P<0.05). In 36 stage Ⅲ patients receiving 6 cycles of Xelox chemotherapy after surgery, CTCs positivity was closely correlated with the chemotherapy sensitivity. Patients with an increased change in CTC status had poorer prognosis than those with decreased changes(P<0.05). Conclusions: Evaluation of CTCs has the value of predicting chemotherapy response in patients with gastric cancer. [ABSTRACT FROM AUTHOR]

Objective: To study the comparative study of four serological detection methods of brucellosis (Brucellacapt), tiger red flat agglutination test (RBT), test tube agglutination test (SAT) and indirect enzyme linked immunosorbent assay (iELISA) for the detection of brucellosis. Methods: Blood samples were collected from 110 suspected cases of brucellosis in the past two years, and then serological tests for Brucellacapt, RBT, SAT and iELISA were carried out in four sera. The diagnostic method of brucellosis in the guidelines for the diagnosis and treatment of brucellosis made by the Ministry of health was the diagnostic gold standard. The results were compared with the results of the diagnosis, and the value of serum detection methods for brucellosis was evaluated and compared.Results: Among the 110 suspected cases, 91 were brucellosis positive and 19 were negative. Brucellacapt test was positive 89 cases and negative 21 cases. RBT test was positive 79 cases and negative 31 cases. SAT test was positive 71 cases and negative 39 cases. iELISA test was positive 82 cases and negative 28 cases. The sensitivity, coincidence rate, Kappa value and the area under the ROC curve of Brucellacapt test were the largest. The iELISA test, the RBT test and the SAT test decrease in turn; the area under the ROC curve of the iELISA test was the largest, the Brucellacapt test was the next, the next was the RBT test, and the minimumarea under the ROC curve of the SAT test was the smallest. Conclusion: The four serological testing methods of Brucellacapt, RBT, SAT and iELISA have certain value for detection of brucellosis. RBT test and SAT test can be used for routine ordinary patients. The sensitivity of Brucellacapt test and iELISA test can be used for patients with suspected cases. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To establish a sensitive method for determination of concentration of lamotrigine (LTG) in rat plasma and to study the pharmacokinetics by LC-MS/MS. Methods 12 rats were divided evenly into model group and shame-operated group. LTG was given as single dose of 10 mg/kg via intragastrical administration. Blood samples were collected from orbital saphenous venous plexus at 5 min,0.25,0.5,1,2,4,6,8,12,24 and 36 h after dosing. The LTG concentrations in rat plasma were assayed by LC-MS/MS. The pharmacokinetic parameters were calculated by DAS software. Results In shame-operated group,cmax (1 382.87±61.17) μg/L,t1/2 (40.43±6.77) h; AUC0-∞(123.45±70.70) mg·h/L. In model group, cmax(1 713.50±65.11) μg/L, t1/2(73.72±17.46) h, AUC0-∞(188.15±76.37) mg·h/L. Conclusion The method is proved to be suitable for the determination of LTG in rat plasma. LTG concentration reached peak value at 2 h in both groups. However, model group had a longer t1/2 and higher concentration than that in shame-operated group, which is a valuable information for further pharmacodynamics study. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the expression characteristics of Treg, Tr1, and Th17 cells in the peripheral blood of patients with chronic hepatitis B (CHB) and their correlations with the state of CHB. Methods A total of 40 patients with mild - to - moderate CHB and 40 patients with severe CHB who were admitted to The 180th Hospital of PLA from January 2013 to June 2014 were included into this study, and 40 healthy people with normal liver function were recruited as control group. Peripheral blood was collected from CHB patients and healthy controls, and then the percentages of Treg, Tr1, and Th17 cells in peripheral blood were determined by flow cytometry. The levels of Foxp3 mRNA and RORɣt mRNA in serum were measured by real - time PCR, and the expression levels of TGF - β1, IL - 10, and IL - 17 were measured by ELISA. Comparison of continuous data between the groups was made by one - way ANOVA analysis and LSD - t test. ResiUts (1) Compared with the control group, the two groups of CHB patients had significantly higher percentages of Tr1, Treg, and Th17 cells (P < 0.05), and had significantly higher levels of Foxp3 mRNA and RORɣt mRNA (P < 0.05) and levels of TGF - β1, IL - 10, and IL - 17 (P < 0.0 5), while the two groups of CHB patients had significantly lower Treg/Th17 ratio (P < 0.05) and Foxp3/RORɣt ratio (P < 0.05) than the healthy controls. (2)The patients with severe CHB had significantly higher percentages of Treg, Tr1, and Th17 cells (P < 0.05), and had significantly higher levels of Foxp3 mRNA and RORɣt mRNA (P < 0.05) and levels of TGF - β1, IL - 10, and IL - 17 (P < 0.05), as compared with the patients with mild - to - moderate CHB, while the patients with severe CHB had significantly lower Treg Th17 ratio (P <0.05) a d Foxp3/RORɣt ratio (P <0.05) than those with mild - to - moderate CHB. Conclusion The expression imbalance of Treg cells (especially Tr1 cells) a d Th17 cells in CHB patients is positively correlated with the progression of the disease; therefore, it may be important to correct the expression imbalance of Treg cells and Th17 cells in the treatment of CHB. Meanwhile, monitoring the percentages of Treg cells (especially Tri ceHs) and Th17 ceHs is helpful for assessing the clinica outcomes of CHB patients and guiding the clinica treatment. [ABSTRACT FROM AUTHOR]

Inaccurately blood sampling conducted by nursing staff is the main influence on blood specimen quality. Redrawing blood specimen may delay therapy and endangers patient safety significantly. The accuracy in blood sampling was only 50.7% from January 7th to 13th, 2008, due to: lack of blood drawing techniques and insufficient training in blood sampling, deficient knowledge on choosing sampling devices, patient body malorientation, scanty subsidiary instruments for blood specimens handling and lack of portable specimen containers. From January 28th to May 7th. 2008, we created and started regulating blood drawing standards, making teaching demonstration videos, arranging on-the-job training and designing adjunct tools for blood sampling. These improvements increased the blood sampling accuracy rate from 50.7% to 98.2%. The project successfully improved patient safety and nursing care quality. [ABSTRACT FROM AUTHOR]

Background & Problems: Morning blood sampling, conducted around 4am to 5am, is routinely ordered due to nothing-by-mouth (NPO) requirements and clinical practice norms. However, this routine may interrupt the normal sleep of patients and decrease the satisfaction of patients with the care received. However, our night-shift staff is not able to take all blood samples during this time, resulting in a high rate of overdue reporting., Purpose: To reduce the overdue rate of laboratory result from 62.3% to less than 19.3%., Resolution: In 2013, we performed interventions including the process reengineering of blood sampling to meet the needs of laboratory result flows and the upgrading of equipment in our neurology ward. The reengineering strategies used included the redistribution of blood sampling times in order to decrease testing for unnecessary items. The equipment upgrades improved the ability of the carrier in the pneumatic tube systems to carry the chilled blood samples. An independent two-sample t-test was used to compare the overdue rate before and after the interventions., Results: The overall overdue rate for the blood sampling to laboratory flow improved from 62.3% before the intervention to 18.0% (t = 7.07, p < .001) after the intervention. Furthermore, the overdue rate for the testing done at the stat laboratory improved from 66.9% to 21.3% (t = 7.36, p < .001), while the overdue rate for the testing done at the non-stat laboratory improved from 52.4% to 17.8% (t = 5.37, p < .001). Besides, the satisfaction of both inpatients and nurses with regard to blood-sampling flow improved from 51% and 54.2%, respectively to 93% (t = 51.10, p < .001) and 94% (t = 12.26, p <.001)., Conclusions: Our process reengineering strategies and the upgrading of the pneumatic tube system successfully reduced the overdue rate for the blood sampling and increased the satisfaction of inpatients.