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1. The effects on antibiotic production in a hetrologous system by sare4854, a novel member of streptomyces actibiotic regulatory protein family (SARP) from a marine microorganism Salinispora arenicola CNH643 DSM 44819.

Author

SHU Xiao, YU Jinlong, WU Shaowen, ZHANG Wei, MA Yanling, ZHU Minzhe, XIA Sisi, XIA Shichao, ZHANG Hao, LI Aiying, and QI Chao

Subjects
STREPTOMYCES, BACTERIAL transformation, DNA-binding proteins, BIOSYNTHESIS, ARENICOLA
Abstract

Streptomyces antibiotic regulatory proteins (SARP) normally play positive regulatory roles during streptomyces antibiotics biosynthesis. sare4854, a gene in Salinispora arenicola CNH643 DSM 44819 was proposed to encode a SARP. Here, sare4854 was expressed in a heterolohous system for functional characterization. However, the antibiotic production in resultant strain was repressed significantly. Bioinfomatic analysis showed that sare4854 has an additional nucleoside triphosphate hydrolases (NTPase) domain at the C-terminus, besides a SARP-like domain (DBD: DNA binding domain and BTA: bacterial transcnptional activation domain) at the N-terminus conserved in all SARPs. The possible mechanism of sare4854 in antibiotic synthesis in this abnormal phenomenon was discussed here. [ABSTRACT FROM AUTHOR]

Published
2014

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