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Objective: To investigate the expression and clinical significance of 14-3-3 θ protein in human nasopharyngeal carcinoma. Methods: Expression status of 14-3-30 was detected by immunohistochemistry in 72 cases of human nasopharyngeal carcinoma and normal nasopharyngeal tissues. Expressions and correlations of 14-3-3 θ were analyzed. Results: In 72 cases of nasopharyngeal carcinoma and normal nasopharyngeal tissues, the positive expressions of 14-3-3 θ were 69.4 % and 15.2 %. The chi square test suggested that 14-3-3 θ expression was correlated with clinical stages (P=0.045). lymph node metastasis (P<0.001). recurrences (P=0.041). while it was not correlated with age (P=0.444). sex (P=0.795). histologic subtype (P=0.468). Correlation analysis suggested that 14-3-3θ expressions were positively correlated with clinical stages (r= -0.256. P=0.030). lymph node metastasis (r=0.506, P<0.001). recurrences (r=0.106. P=0.033). while it was negatively correlated with age. sex, and histologic subtype (P>0.05). Conclusion: In human nasopharyngeal carcinoma, expressions of 14-3-3θ were increased and correlated with clinical stages and metastasis. [ABSTRACT FROM AUTHOR]

Objective: In order to further study the effect of biology behavior of the gene 14-3-3θ to breast cancer, to build a recombinant vector and breast cancer cell line which can over express gene 14-3-3θ. Methods: The gene 14-3-3θ in the pENTR 3C DUAL is synthesized by a company, we put the gene 14-3-3θ into the vector pcDNA4.0 which can product 14-3-3θ in eukaryotic cells by recombinant DNA technology and then transfect the 293T, we identify the overexpression of the recombinant vector by the western blot and PCR. At last, we transfect the breast cancer cell by the recombinant vector and screen the breast cancer cell with Zeocin. Results: Successfully construct the recombinant vector and breast cancer cell line which can over express 14-3-3θ. Which shows that gene 14-3-3θ may play an important role in the early diagnosis and prognosis of breast cancer prediction. [ABSTRACT FROM AUTHOR]