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为了筛选出适合辣椒鸡油的天然抗氧化剂，以延长其保质期，研究并比较了6种天然抗氧化剂（迷迭香提取物、维生素E、茶多酚、维生素C、槲皮素和硫辛酸）对辣椒鸡油酸值、过氧化值、DPPH自由基清除能力和总还原能力的影响。结果表明：6种天然抗氧化剂在辣椒鸡油中均具有一定的抗氧化能力；在抑制酸值方面，槲皮素效果最佳，维生素C最差;在抑制过氧化值方面，槲皮素效果最佳，维生素E最差；在DPPH自由基清除能力方面，迷迭香提取物效果最佳，硫辛酸最差；在总还原能力方面，槲皮素效果最佳，维生素E最差；槲皮素、迷迭香提取物和茶多酚在酸值、过氧化值、DPPH自由基清除能力和总还原能力方面均位于前三。综合考虑抗氧化效果、抗氧化剂稳定性、成本等因素，可优先选择迷迭香提取物和茶多酚作为辣椒鸡油的抗氧化剂以提高其储藏性能。In order to screen natural antioxidants suitable for chili chicken oil and extend its shelf life, the effects of six natural antioxidants (rosemary extract, vitamin E, tea polyphenols, vitamin C, quercetin and lipoic acid) on the acid value, peroxide value, DPPH radical scavenging capacity, and total reducing capacity of chili chicken oil were investigated and compared. The results showed that six natural antioxidants had certain antioxidant capacity in chili chicken oil; in inhibiting acid value, quercetin was the most effective and vitamin C was the worst; in inhibiting peroxide value, quercetin was the best and vitamin E was the worst; in terms of DPPH radical scavenging capacity, rosemary extract was the most effective and lipoic acid was the worst; in terms of total reducing capacity, quercetin was the most effective and vitamin E was the worst. Quercetin, rosemary extract and tea polyphenols were in the top three in terms of acid value, peroxide value, DPPH radical scavenging capacity and total reducing capacity. Considering the antioxidant effect, antioxidant stability and cost, rosemary extract and tea polyphenols can be preferred as antioxidants for chili chicken oil to improve its storage performance.

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Objective To study the mutation types of OprD gene in Pseudomonas aeruginosa resistant to carbapenems but sensitive to cephalosporins （Car-R/Ceph-S）. Methods Ten strains of Pseudomonas aeruginosa which were resistant to carbapenems but sensitive to cephalosporins were isolated and cultured in the First Affiliated Hospital of Kunming Medical University from January 2021 to March 2021. Polymerase chain reaction （PCR） was used to detect the carrying status of OprD gene in Pseudomonas aeruginosa, and the mutation type of OprD gene was analyzed by sequencing. Results All the 10 strains showed obvious OprD bands and the sequencing analysis showed that there were 2 frameshift mutations, 8 amino acid substitutions, 2 early termination codons and 4 large fragment deletions. Conclusion The mutation types of OprD gene in carbapenem-resistant but cephalosporin-sensitive （Car-R/Ceph-S） strains of Pseudomonas aeruginosa are diversified with the highest frequency of point mutations, deletion of large segments and early termination of codons. These mutations lead to changes in the structure of OprD, resulting in the occurrence of drug resistance. [ABSTRACT FROM AUTHOR]

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The purpose of the study was to investigate the effects of different levels of Pfaffia paniculata stem and leaf silage on growth performance, slaughter performance and muscle nutrient composition of Guangxi Ma chicken. Two hundred 80-day-old Guangxi Ma chickens were randomly divided into four groups (control group, group 1, group 2 and group 3) with five replicates per group and 10 chickens per replicate. The control group chickens were fed with basal diet, group 1, group 2 and group 3 chickens were fed content 30%, 40% and 50% Pfaffia paniculata stem and leaf silage, respectively. The experiment lasted for 90 d. The result show that compared with the control group, the results showed that the average daily gain in group 2 was significantly increased (P<0.05), and the feed to gain ratio was significantly decreased (P<0.05). Slaughter percentage and abdominal fat percentage in group 1 were significantly increased (P<0.05), and water content of breast muscle in group 3 was significantly increased (P<0.05). Compared with the control group, the content of aspartic acid, glutamic acid, serine, tyrosine and phenylalanine in the chest muscles of each experimental group were increased, the content of aspartic acid, proline, serine and tyrosine in the leg muscles of each experimental group were increased. The study indicates that the growth performance, slaughter performance and muscle quality of Guangxi Ma chicken were improved to some extent by adding Pfaffia paniculata stem and leaf silage. [ABSTRACT FROM AUTHOR]

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BACKGROUND: Pioglitazone is a common hypoglycemic drug capable of improving proliferation and activation, and inhibiting apoptosis of endothelial progenitor cells (EPCs). We speculated that the combined use of pioglitazone and EPCs transplantation could have significant improving effects on hyperglycemia and kidney function after diabetes mellitus. OBJECTIVE: To investigate the improving effect of EPCs transplantation combined with pioglitazone treatment on the kidney function of diabetic rat models. METHODS: The 15 of 75 Wistar rats were randomly selected and served as normal control group (no treatment). Animal models of type 1 diabetes mellitus were made in the rest 60 rats through the intraperitoneal injection of 40 mg/kg streptozotocin for continuous 5 days. The human EPCs (labeled by CM-Dil) were recovered, cultured and preserved until transplantation. After 4 weeks of modeling, the 60 rat models were randomly divided into model group (PBS injection), pioglitazone group, EPCs transplantation group and combined treatment group, followed by tail vein injection of EPCs suspension and/or intragastric administration of 20 mg/kg pioglitazone for continuous 4 days. After 8 weeks of treatment, the levels of glucose, insulin and creatinine in serum, urea nitrogen and urine protein during 24 hours were determined. The number and distribution of EPCs labeled by CM-Dil were detected by fluorescence microscope, the apoptosis in kidney cells was tested by TUNEL method, and the kidney weight/body weight ratio in rats was calculated. RESULTS AND CONCLUSION: Compared with the model group, the blood glucose and serum creatinine levels and the urea nitrogen and urine protein concentrations during 24 hours were significantly decreased (P < 0.05), and the serum insulin level was significantly increased (P < 0.05) in the pioglitazone and EPCs transplantation groups. These biochemical indexes in the combined treatment group were more significantly altered compared with the model group (P < 0.01). The kidney weight to the body weight ratio was lowest in the combined treatment group and lower in the pioglitazone and EPCs transplantation groups followed by the model group (P < 0.05). The order of apoptotic kidney cells labeled by TUNEL was as follows: model group > pioglitazone group > EPCs transplantation group > combined treatment group (P < 0.05). To conclude, the EPCs transplantation combined with pioglitazone treatment can decrease the blood glucose and serum creatinine levels and urea nitrogen and urine protein concentrations, improve the serum insulin level, reduce cell apoptosis in the kidney, and remit the kidney dysfunction of diabetic rats to a certain extent. [ABSTRACT FROM AUTHOR]

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