1. 植物乳杆菌 LP21源纳米硒的分离纯化研究.
- Author
-
王丽红, 王文斌, and 孔阳
- Abstract
The nano-selenium synthesized by Lactobacillus plantarum LP21 is easy to adhere to the surface of the bacteria and is difficult to separate and extract. In this study, under the premise of maintaining the integrity of the cells structure, the nano-selenium attached to the bacteria was dispersed. The effects of ultrasound.organic solvents and surfactants on the dispersion of nano-selenium were investigated by single factor experiments. The optimal dispersion conditions were determined through orthogonal experiments. Finally, sucrose density gradient and microporous filter membrane filtration were used to separate and purify nano selenium. The results of single factor showed that ultrasonic.organic solvent and surfactant all had dispersion effect among which surfactant SDS had the best effect. The optimum dispersion conditions were determined by orthogonal method to be the addition of 2.5% SDS and 1.5% n-hexanol, and the ultrasonic treatment time was 25 minutes. Separation and purification using 50%, 60%, and 70% sucrose density gradient centrifugation followed by 0. 22 µm water-based microporous filter membrane filtration to obtain pure nano-selenium. The particles were evenly distributed and the average particle size was 165.88±35.35 nm. This study provides a reference for the separation and preparation of nano-selenium from microbial sources. [ABSTRACT FROM AUTHOR]
- Published
- 2024