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[Objective]The symbiotic effect of mixed arbuscular mycorrhizal fungi (AMF) and different inoculation periods were studied in seedlings of Paris polyphylla var. yunnanensis. [Method] The indoor pot test was applied and different mixed AMF were cultivated in the sterilized soil matrix respectively. The differential expression of Symbiosis-receptor-like kinase (SYMRK) and doesn't making fections 1 (DM11) were detected by real-time fluorescence quantitative PCR. [Result] The results showed that Paris polyphylla var. yunnanensis and AMF was a good symbiotic relationship, and the infection rate of mycorrhizal seedlings had reached more than 80. 00%. Among them, the indicators of S6 mixed fungus composed of Gigaspora margarita, Gigaspora gigantea, Scutellospora calospora, etc, were generally higher than other treatments. The activities of alkaline phosphatase (ALP) and succinate dehydrogenase (SDH), and the relative expression of SYMRK and DM11 genes were the highest in phase T4 (two-year seedlings of Paris polyphylla var. yunnanensis). Thus, we believed that the biological activity of Paris polyphylla var. yunnanensis inoculated during phase T4 was better. [Conclusion] In short, The S6 mixed AMF inoculated to the two-year seedlings of Paris polyphylla var. yunnanensis showed the best comprehensive effect for promoting growth of Paris polyphylla var. yunnanensis seedlings. This provides new ideas for the introduction and cultivation in Paris polyphylla var. yunnanensis. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to study the effect on the amount of rhizospheric soil microorganisms and soil enzyme activity of Parisbpolyphylla var.Yunnanens seedlings colonized by Arbuscular mycorrhizal (AM) Fungi. [Method] A pot experiment was conducted and 28 species of AM fungi were inoculated in the seedlings of Paris polyphylla var.yunnanensis. The number of culturable microbes in rhizosphere soil was calculated by the dilution method of plate counting, and the laboratory analysis was used to analyze soil enzyme activity. [Result] The mycorrhizal infection rate of the different AM Fungi treatment groups was in the range of 75% — 100%. The difference of rhizospheric soil microorganism quantity, biomass carbon and soil enzyme activity of Paris polyphylla var.yunnanensis seedlings colonized by different AM Fungi exist.Inoculation with AM fungi increased the number of culturable bacteria and actinomycetes, decreased the number of culturable fungi in rhizosphere soil, and increased the utilization rate of the soil microbial carbon source.It also changed the richness and homogeneity of species in soil and increased the biodiversity of soil. The activities of soil enzymes (protease, urease, phosphatase, catalase and sucrase) in the rhizosphere of Paris polyphylla var.yunnanensis seedlings were increased, especially the activities of phosphatase and sucrase. [Conclusion] AM fungi affected the rhizosphere soil microenvironment of Paris polyphylla var.yunnanensis seedlings in different degrees and provided important theoretical and practical values for the artificial cultivation and application of AM fungi. [ABSTRACT FROM AUTHOR]