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In recent years, the unreasonable use of cultivated land in China has caused a series of serious problems. Therefore, the state has put forward an important measure of "Exploring Pilot Program of Farmland Rotation and Fallow System ". Labor force is one of the main factors of agricultural production. Therefore, it is of strategic significance to further promote the national rotation fallow policy, modernization and urbanization to sum up the situation of farmer's rotation fallow land and labor force transfer and grasp the effect of fallow area on labor force transfer. Based on this, this paper uses the relevant survey data of 1542 households in six counties of Hebei, Gansu and Yunnan provinces to analyze the situation of farmer's rotation fallow land and labor transfer, and uses Tobit regression model to quantitatively analyze the impact of fallow area on labor transfer. The results show that: (1) There is a difference between the ratio of fallow households and fallow area per household in the study area, and the order of which is both Gansu > Yunnan > Hebei; (2) 62.97% of the households in the study area have the phenomenon of labor transfer, and the number of labor transfer of fallow households far exceeds that of non-fallow households; (3) The core variable, fallow area, has a significant positive impact on labor transfer; the control variable, the total household population, gender of householder, labor education level, medical expenditure, the distance from residence to highway and average wage per labor force have a significant positive impact on labor transfer, while the dependency ratio has a significant negative impact on labor transfer. [ABSTRACT FROM AUTHOR]