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[Objective] Screening and obtaining the antagonistic actinomycetes with significant antifungal activity against the pathogen of Sarcandra glahra, for the theoretical foundation for biological control of anthracnose. [Method] The actinomycetes were isolated by root soil of health S. glabra using spread plate method, and the highest antifungal activity of strain was screened by the method of plate confrontation, and using in vitro leaf stabbing wound inoculation method to screen the control effect of antagonistic strain against anthracnose of S. glabra. Moreover, the species was identified through morphological characterization and 16 S rDNA sequence by establishing a phylogenetic tree to analyze its classification status. [Result] By plate confrontation and antibacterial spectrum test, the results showed that the JT-2 F was isolated from soil had the highest antagonistic effect against anthracnose of S. gfubra, resulting in the hyphae being swelled and deformed, the antagonism rate was 86. 75%. Antibacterial spectrum tests showed it had antagonistic activities to 14 plant pathogens, whose average inhibition rate was 81. 50%. Based on morphological characters and 16S rDNA sequence analysis, strain JT-2F was identified as Streptomyces tsukiyonensis. In vitro control effect test results showed that the fermentation broth of JT-2F strain had 87. 32 % control effect on anthracnose of S. glabra. [Conclusion ] JT-2F has development and application potential as biocontrol material for anthracnose of S. glabra. [ABSTRACT FROM AUTHOR]