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Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Antibiotic resistance in bacteria is a major public health concern. Antibiotic resistance genes (ARGs) may be horizontally transferred between human, animal, and environmental microbiomes, potentially linking their interdependent ecosystems to public health. Birds, being among the most diverse species on the planet, can facilitate the global spread of resistance genes through their long-distance dispersal, consequently posing significant health risks. This paper reviews how wild birds can actively mediate the transmission of ARGs in ecosystems and proposes their significant roles as transmission agents. It then describes the distinct roles of urban resident birds and migratory birds in the transmission of antibiotic resistance genes. Particularly, the review highlights the pivotal role of migratory birds in the long-distance transmission of ARGs. Therefore, researching the transmission of ARGs mediated by wild birds emerges as a critical direction. [ABSTRACT FROM AUTHOR]

Copyright of Architectural Journal / Jian Zhu Xue Bao is the property of Architectural Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The present paper aimed to study the physiological changes and gene dynamic changes of rice under low temperature stress, which was helpful to analyze the physiological basis and molecular mechanism of rice response to low temperature stress, and laid a theoretical foundation for screening suitable rice variety with cold tolerance and studying how to improve the cold-tolerance inheritance of rice. [Method] Taking three rice varieties with different cold-tolerance levels, P427 ( strong cold-tolerance), Nipponbare (cold-tolerance), 9311 (cold-sensitivity) as testing materials, and using method of phenotype combined with physiochemical index, the paper studied the effect of 3 -4 °C low temperature stress on phenotype, chlorophyll content and electric conductivity, and used RNA-Seq to analyze enrichment pathways and the dynamic change of gene response to low temperature stress. [Result] The rice variety with strong cold-tolerance P427 showed strong adaptability to low temperature. It maintained higher chlorophyll content under low-temperature stress, and its chlorophyll content before low temperature treatment, on the third day of low temperature treatment, on the third day of recovery growth was 29. 64, 29. 30 and 27. 96 µgig, respectively. Its electric conductivity on the first day, third day, fifth day of low-temperature was 10. 39%, 13. 08% and 17. 04%, and its membrane system had the lightest damage. A large number of genes were differentially expressed in both cold tolerant and cold sensitive varieties under low temperature induction, and the number of differentially expressed genes ( DEGs) continued to increase with the extension of low temperature treatment time,but the number of specific DEGs in P427 decreased from 2408 to 1717. In addition, only 576 specific genes of P427 were differentially expressed from 1 days to 3 days under low temperature treatment. GO and KEGG enrichment analysis showed that the specific DEGs of P427 after low temperature treatment were mainly enriched in metabolic pathways such as biosynthesis of secondary metabolites, plant hormone signal transduction and phenylpropane biosynthesis. [ Conclusion] There may be differences in cold-tolerance response genes and regulatory mechanisms among cultivars with different cold tolerance. There was a set of cold-tolerance response genes in P427 cultivar with strong cold tolerance, which was different from that in Nipponbare and 9311. Plant hormone signal transduction and phenylalanine metabolism were significantly affected by low temperature stress in seedling rice. P427 may respond to cold tolerance of seedling rice through metabolic pathways such as biosynthesis of secondary metabolites, plant hormone signal transduction and phenylpropane biosynthesis pathway. [ABSTRACT FROM AUTHOR]

Copyright of Architectural Journal / Jian Zhu Xue Bao is the property of Architectural Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cottonseed oil, soft in taste and golden in color, plays a crucial role in various foods. In addition, cottonseed oil has attracted much attention due to its unique fatty acid properties, anti-inflammatory and heart protective properties. Therefore, it is of great significance to understand the genetic mechanism that regulates the biosynthesis of cottonseed storage oil, carry out key gene mining, and select high-quality cotton varieties with high oil content. With the rapid development of high-throughput sequencing technology and bioinformatics analysis tools, whole genome sequencing has become simpler and more efficient, genome-wide association analysis (GWAS) has become a key tool for studying complex traits. This paper introduces the principle and research advantages of GWAS, discusses the progress of its application in the research of different oil crops and cotton characters. Some suggestions for future genetic studies of cottonseed oil related traits were put forward. The existing problems were discussed and prospected in order to provide a theoretical basis for future studies on the genetic basis of cottonseed oil traits and marker-assisted breeding by GWAS. [ABSTRACT FROM AUTHOR]

Copyright of Biotechnology Bulletin is the property of Biotechnology Bulletin Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Epilepsy is a common neurological disease in clinic. Epilepsy caused by structural causes can be cured by surgery, which has been the focus of attention in the field of epilepsy surgery. With the development of gene sequencing technology, more and more studies have found that structural etiology may be caused by somatic mutations of genes. In this paper, somatic mutation-related pathogenic genes of four common pathological types of epilepsy surgery, including malformation of cortical development (MCD), long-term epilepsy associated tumor (LEAT), hippocampal sclerosis (HS) and hypothalamic hamartoma (HH), were reviewed, and their effects on surgical protocol formulation and prognosis were reviewed, in order to provide a new idea for the treatment of epilepsy. [ABSTRACT FROM AUTHOR]

Non-small cell lung cancer (NSCLC) is a prevalent tumour type in our country, with lung squamous carcinoma being a commonly observed NSCLC subtype besides lung adenocarcinoma. Epidermal growth factor receptor (EGFR) is a significant driver gene in lung cancer, and EGFR mutation frequency is considerably lower in lung squamous carcinoma in comparison to lung adenocarcinoma. Although targeted therapy against EGFR has demonstrated significant advancements in lung adenocarcinoma, while progress in lung squamous carcinoma has been relatively sluggish. This paper reviews recent studies on molecular targeted therapy for EGFR-mutated lung squamous carcinoma and summarises the efficacy of EGFR-tyrosine kinase inhibitors (TKIs) in treating squamous carcinoma of the lung, in order to provide a reference for treating patients with EGFR-mutated squamous carcinoma of the lung. [ABSTRACT FROM AUTHOR]

Copyright of Systems Engineering & Electronics is the property of Journal of Systems Engineering & Electronics Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objectives]This paper aimed to study the responses of soybean GmCLC-d1/d2 genes and their promoters to NaCl stress, and to explore their physiological functions in salt stress adaptation in plants. [Methods]GmCLC-d1/d2 genes were cloned from the soybean ‘Lee68’cultivar, their plant RNA interference (RNAi) and overexpression vectors were constructed for obtaining RNAi-and overexpressed (OE) -GmCLC-d1/d2 soybean hairy root composite plants mediated by Agrobacterium rhizogenes and OE-GmCLC-d1/d2 Arabidopsis thaliana mediated by A. tumefaciens,respectively. The changes in plant morphological and physiological indexes under NaCl treatment were analyzed and compared. [Results]Compared with the empty vector-transformed soybean hairy root composite plants under NaCl stress, the plant fresh weight, leaf relative water content (RWC) and chlorophyll content, activities of catalase (CAT),peroxidase (POD) and superoxide dismutase (SOD) in roots and leaves of RNAi-GmCLC-d1/d2 soybean plants obviously decreased, the relative electrolytic leakage (REL) and malondialdehyde (MDA) content in roots and leaves, and Cl-content in roots, stems and leaves apparently increased, but NO-3 content significantly decreased, which resulted in Cl-/NO-3 ratios in roots, stems and leaves, particularly raised significantly in the shoots. However, the above indexes were opposite in OE-GmCLC-d1/d2 soybean hairy root composite plants, with the enhanced salt tolerance. The seed germination rate, seedling root length, and 3-week-old plant fresh weight, leaf RWC and chlorophyll content of OE-GmCLC-d1/d2 A. thaliana were clearly better than those of wild type (WT) under salt stress, and additionally, REL and MDA content in shoots significantly decreased, the activities of CAT,POD and SOD were improved, while Cl-contents in roots and shoots decreased, and NO-3 content obviously increased, which resulted in a significant drop of Cl-/NO-3 ratio in roots and shoots, especially in the latter. [Conclusions]GmCLC-d1/d2 genes could facilitate NO-3 uptake in the roots of overexpressed soybean hairy root composite plants or A. thaliana plants, and its transport and distribution to the shoots under salt stress, and simultaneously inhibit Cl-uptake and transport to a certain extent, so as to maintain lower Cl-/NO-3 ratio, especially in the shoots, for enhancing the salt tolerance, and among them, the effect of GmCLC-d2 gene was more obvious. [ABSTRACT FROM AUTHOR]

Polygonatum cyrtonema seeds have a comprehensive dormancy phenomenon, and analyzing the changes of key genes before and after seeds germination plays an important role in exploring seed physiology and breaking dormancy. Based on high-throughput sequencing technology (Illumina Hiseq 2500), the transcriptome sequencing and bioinformatics analysis were carried out in four stages of P. cyrtonema seed germination with three biological replicates. The results were as follows: (1) 388 231 Transcripts and 178 319 Unigenes were obtained by de novo assembly. (2) Among the 11 817 Unigenes with significant differences before and after germination, 6 405 were up-regulated and 5 412 were down-regulated. (3) Significant difference genes were enriched and analyzed in the GO enrichment analysis, and the differential expression up- and down-regulation Uningenes were main enriched in biological process and molecular function, which were mainly involved in metabolic processes and catalytic activities. (4) KEGG significant enrichment indicated that the differential expression genes were mainly enriched in ribosome, plant hormone signal transduction, starch and sucrose metabolism and other pathways; the up-regulated differential genes were mainly enriched in ribosome 231, plant hormone signal transduction 56, starch and sucrose metabolism 76 and others; the down-regulated differential genes were mainly enriched in phenylpropanoid biosynthesis 48 plant hormone signal transduction 48, starch and sucrose metabolism 48, and other pathways; a total of 40 differential genes were involved in the synthesis of auxin pathway key enzymes. Genes encoding sucrose phosphate synthase were down-regulated and genes encoding glycogen phosphorylase were up-regulated in starch and sucrose metabolic pathways. This paper preliminarily clarified that plant hormone signal transduction, starch and sucrose metabolism played a key role before and after the seeds germination of P. cyrtonema and analyzed the key genes involved in these two pathways. This study preliminarily clarifies that plant hormone signal transduction and starch and sucrose metabolism play a key role before and after the germination of P. cyrtonema seeds, and analyzes the key genes involved in these two pathways, which provides a reference for further research on the physiology, reproduction and breeding of P. cyrtonema seeds. [ABSTRACT FROM AUTHOR]

Aiming at the problems of low efficiency and unstable solutions of traditional genetic algorithm (GA) in solving traveling salesman problem (TSP), this paper proposed a novel genetic algorithm based on genes pool (GPGA). GPGA searched for edges that decreased the fitness of Hamiltonian cycles from the population and constituted a gene pool as excellent genes. The parent generation reproduced the better offspring of Hamiltonian cycles under the guidance of the gene pool. The gene pool also synchronously updated according to the better Hamiltonian cycles and helped the current Hamiltonian cycles evolve to the optimal Hamiltonian cycle step by step. The computational results demonstrate that GPGA is better than traditional genetic algorithm and several improved genetic algorithms under the same preconditions. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The influence of genes on pork quality traits is one of highlights in the research of genetics and breeding nowadays. The paper reports the research status of effect on genes related to pork quality at home and abroad, briefly introduces the related technologies of genetic testing, focusing on the halothane gene, rendement napole gene, myogenic family and other genes and their mechanism of action, to provide a scientific theoretical basis for the improvement of pork quality in the future. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Lung cancer associated with cystic airspaces (LCCA) is a type of lung cancer characterized by the presence of cystic cavities in or around the tumor on imaging. Due to its high potential for misdiagnosis or underdiagnosis, the prognosis of LCCA patients is poor, necessitating further large-scale clinical studies to elucidate its characteristics. Currently, four imaging classification systems exist, and there has been a progressive increase in attention towards LCCA, particularly with regard to the study of its imaging features. The results indicate a correlation between the pathological features and imaging findings of LCCA; however, research on driver gene mutations and molecular subtyping associated with lung cancer remains insufficient. Due to the challenges associated with early diagnosis and the poorer prognosis compared to general types of lung cancer, this paper comprehensively reviews the research progress on LCCA, including its definition, etiology, pathogenesis, imaging features, histological and pathological features, treatment, and prognosis, aiming to serve as a valuable resource for clinical decision-making. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Application Research of Computers / Jisuanji Yingyong Yanjiu is the property of Application Research of Computers Edition and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To evaluate the safety of clopidogrel in the patients with acute coronary syndrome (ACS) by meta analysis based on ABCB1 C3435 Tgene polymorphism. Methods: The databases, such as Wanfang Data, the Chinese Biomedical Literature Database (CBM), the Chinese Journal Fulltext Database in China National Knowledge Infrastrusture (CNKI), the China Science and Technology Journal Database (VIP), the Science Citation Index (SCI), PubMed and EMBase, were searched from the inception to December 2019. The quality of the collected literature was evaluated, and then meta analysis was performed on the obtained data by using Stata 14.0. Results: Finally, 13 papers were included and 12 970 patients were enrolled for study. Meta analysis showed that there was no significant difference in the incidence of major adverse cardiac events (MACE) among the subgroups based on ABCB1 C3435 T gene polymorphism (P>0.05) ; in the allele gene model OR = 1.11, 95% CI (0.94, 1.30), in the dominant gene model OR = 1.03, 95% CI (0.82, 1.31) and in the recessive gene model OR = 1.13, 95% CI (0.99, 1.30). In the recessive gene model, there was a significant difference in the incidence of MACE of the patients who received a clopidogrel loading dose of 300 mg (P<0.05) and there was also a significant difference in the incidence of MACE in the European population (P<0.05). And in all MACE, there was a significant difference in the incidence of myocardial infarction (P<0.01). Conclusion: There was no correlation between polymorphism of ABCB1 C3435 T gene and MACE incidence, however correlation could be seen in the incidence of MACE and ABCB1 C3435 T gene polymorphism, when the patients received a clopidogrel loading dose of 300 mg and in the European population. [ABSTRACT FROM AUTHOR]

Digital PCR (dPCR), an absolute quantitative method of DNA molecules, has been successfully applied for genetically modified organisms (GMO) detection and reference material characterization. In this paper, a duplex ddPCR method was established for quantification of MON87427 content with reference plasmid pUC57-M harboring MON87427-specfic sequence and 5 maize reference gene sequences as quality control. By comparing the 5 reference genes, zSSIIb was determined to combine with MON87427 for duplex ddPCR assay according to the resolution of positive droplets and negative droplets, the number of raindrops with medium signal intensity, and the consistency between measured value and theoretical value. The optimal annealing temperature for MON87427/zSSIIb duplex ddPCR was determined at 58.4°, and the duplex ddPCR displayed good linearity over the range from 10 to 60 000 copies. The quantitative results of blinded samples by duplex ddPCR were well comparable with those by real-time quantitative PCR (qPCR), indicating that the MON87427/zSSIIb duplex ddPCR might replace the qPCR method for quantification of transgenic maize MON87427 and characterization of MON87427 reference materials. [ABSTRACT FROM AUTHOR]

To break the main obstacle that the cell wall preventing delivery of exogenous biomolecules into intact cell during plant genetic transformation, nanomaterial-mediated gene delivery technology was currently successfully applied. Traditional transgenic methods require the aid of external force or the mediation by creatures like agrobacterium. The nano-carriers for genetic delivery possess outstanding advantages such as excellent biocompatibility, ability to protect external DNA/RNA against nuclease degradation, high transfection efficiency, thus provide a new promising way for plant genetic engineering. In this paper, we summarized the bottlenecks of plant genetic transformation met currently, the drawbacks of the existing methods and the advantages of nanomaterial-mediated delivery. And the key techniques and research progresses of nanoparticles-mediated plant transformation were discussed in detail. Ultimately, the advantages and disadvantages as well as future direction and problems of this newly formed technology were also discussed and prospected. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Intelligent Agriculture Mechanization is the property of Nanjing Institute of Agricultural Mechanization and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In recent years, with the rapid development of gene sequencing technology, a variety of epilepsy pathogenic genes have been discovered, among which GRIN2A gene variations encoding the neurotransmitter N-methyl-D-aspartate receptor (NMDAR) are closely related to the occurrence of epilepsy. At present, there are relatively few studies on the function of epileptic related variations in GRIN2A gene. The resulting epilepsy phenotypes are complex and varied, and different patients have different therapeutic effects on different drugs. This paper briefly summarizes the possible relationship between the changes of NMDAR structure and function caused by GRIN2A gene variations and the clinical phenotype of epilepsy, providing clues for the precise treatment of epilepsy. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective Summarize the clinical and genetic characteristics of atypical Rett syndrome and developmental epileptic encephalopathy caused by IQSEC2 gene variation. Methods and Results From May 2020 to April 2022, Anhui Provincial Children's Hospital diagnosed and treated 4 children with atypical Rett syndrome and developmental epileptic encephalopathy caused by IQSEC2 gene variation, including 2 males and 2 females were a pair of identical twins. They all had comprehensive developmental delay before onset. At the age of 2 years, all cases gradually exhibited clinical manifestations of atypical Rett syndrome, such as frequent clapping, biting, sleep disorders (increased sleep or difficulty falling asleep), and grinding teeth, followed by developmental regression and seizures. The initial age of epilepsy was from 2 years and 2 months to 2 years and 10 months. All cases started with generalized tonic-clonic seizure, with epileptic spasm occurring between 2 and 11 months of course. Case 2, Case 3 and Case 4 were also accompanied by focal seizures. Four cases with VEEG background of 4-6 Hz θ wave, the VEEG during the interictal phase was a broad multifocal sharp slow complex wave. In Case 2, Case 3 and Case 4, MRI was abnormal, mainly with increased depth of cerebral hemispheric sulcus and gyrus. The whole exome sequencing suggested pathogenicity and possible pathogenic variations in the IQSEC2 gene, Case 1 and Case 2 were frameshift mutations of c. 608dup (p. Gln204Profs*35), while Case 3 and Case 4 were nonsense mutations of c.2231C>A (p.Ser744Ter) and c.2521C>T (p. Gln841Ter), respectively. The four mutation sites have not been reported domestically or internationally. All cases received treatment with multiple antiepileptic seizure medicine. The last follow-up age was from 4 years and 3 months to 6 years and 3 months. All cases were unable to walk alone and had no active language. There were no seizures in Case 1 for 3 years, occasional seizures in Case 2 and Case 4, and frequent seizures in Case 3. Conclusions IQSEC2 gene variation can manifest as atypical Rett syndrome, which can be accompanied by refractory epileptic spasms. Both males and females have severe phenotypes, and the severity of clinical phenotypes at the same mutation site varies. Our report enriches the variation spectrum and clinical phenotype spectrum of the IQSEC2 gene, expands the genetic spectrum of Rett syndrome and developmental epileptic encephalopathy, and provides value for the clinical diagnosis, treatment and subsequent research of this disease. [ABSTRACT FROM AUTHOR]

Ganoderma lucidum is an edible and medicinal fungus with a wide range of applications. Ganoderic acids are not only one of the main medicinal components of G. lucidum,but also an important indicator to measure its economic value. Although the biosynthetic pathway of ganoderic acids was clear, the molecular regulation mechanism had not been revealed. Based on the development of genomics, proteomics and progress on genetic methods in recent years, this paper reviewed the functional genes, signal molecules(such as reactive oxygen species, nitric oxide, calcium ions)and environmental factors(such as high temperature stress, nutrient stress, chemistry inducement)that played an important role in the ganoderic acid biosynthesis. It indicated that the biosynthesis of ganoderic acid was regulated by multiple factors. This research can provide theoretical basis and technical guidance for the cultivation of high-quality G. lucidum. Also, it is of great significance for improving the competitiveness of edible and medicinal fungal products in the international market. [ABSTRACT FROM AUTHOR]

Existing clustering ensemble algorithm starts from the perspective of cluster members, if all the cluster members are used, the ensemble result is affected by the inferior members. If the cluster members are selected and then used in ensemble, the selected strategy has subjectivity. To avoid these two limitations to some extent, from the perspective of elements, this paper proposed a new clustering fusion method. It selected a part of class-determined elements through multi-granulation rough sets with incongruous decisions, and then used this part of the elements to generate a new clustering. Multi-granulation rough set model with incongruous decisions could describe the phenomenon of inconsistent decisions with consistent attribute set. There fore, this paper proposed a model of multi-granulation rough set with incongruous decisions and a clustering ensemble algorithm based on the model. First of all, it ran a K-means clustering algorithm several times on the data set in the case and gene rated multiple granule structures. Next, it calculated inclusion degrees among all the granulations, and obtained the matrix of inclusion degree. Used Otsu's method togenerate a threshold, then got several group of granulation that met the threshold condition. According to the model of multi-granulation rough set with incongruous decision, it obtained lower and upper approximations . Finally, classified the elements of lower approximation and boundary separately to obtained a clustering that has been fused. The experiments show that the algorithm has a high time efficiency and robustness, which improves the result of K-means clustering. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forensic Science & Technology is the property of Institute of Forensic Science, Ministry of Public Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The present paper aimed to clarify the distribution of rice blast resistance genes Pib, Pi9, Pi2, Pi54 and Pish in Japonica rice varieties (lines) and the resistance levels of different genotype to rice blast. [Method] A total of 93 rice materials were assessed by artificial inoculation and screening of molecular markers tightly linked to 5 major resistance genes. [Result] Three, two and one resistance genes were found in 19 (20. 4%), 25 (26. 9%) and 28 (30. 1%) materials, respectively, and five resistance genes corresponding to markers were found in all varieties (lines). There were 49 (52. 7%), 16 (17. 2%), 8 (8. 6%), 31 (33. 3%) and 31 varieties (lines) (33. 3%) containing rice blast resistance gene Pib, Pi9, Pi2, Pi54 and Pish in 93 varieties (lines), and the genetic frequency of Pib was the highest within this collection. A total of six genotypes and gene combinations (Pi9, Pi54, Pib + Pi9, Pib + Pi2, Pib + Pish and Pi9 + Pi54) are applicable in the rice-breeding, due to the resistance to rice blast but with low distribution in the tested varieties (lines). [Conclution] The study provided a scientific basis for breeding rice cultivars with rice blast-resistance. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background Owing to the clinical variability and molecular heterogeneity, the traditional Sanger sequencing takes time and effort and is inefficient. in this paper, target region capture sequencing in the diagnosing of autosomal recessive Charcot - Marie - Tooth disease (AR - CMT) is explored. Methods Clinical data and peripheral blood sample of 5 clinically suspected AR - CMT patients were collected. Charcot-Marie-Tooth disease (CMT) related gene mutation were detected by target region capture sequencing. The candidate variants were confirmed in the peripheral blood sample of the patients and their parents by Sanger sequencing. Results Target region capture sequencing revealed that compound heterozygous mutations of GDAP1 gene was found in 2 patients, and was not seen in the other 3 patients. Sanger sequencing revealed that among the 2 patients with mutation of GDAP1 gene, compound heterozygous mutation c.767A > G (p. His256Arg) and c.866T > A (p. Phe289Tyr) were seen in Case 1, whose father carried c.866T > A (p. Phe289Tyr) heterozygous mutation and mother carried c.767A > G (p. His256Arg) heterozygous mutation, while compound heterozygous mutation c.571C > T (p. Arg191X) and c.589delC (p. Asp198ilefsX8) were seen in Case 2, whose father carried c.589delC (p. Asp198ilefsX8) heterozygous mutation and mother carried c.571C>T (p. Arg191X) heterozygous mutation. Therefore, Case 1 and Case 2 were all diagnosed as AR - CMT. Conclusions Target region capture sequencing is a rapid and reliable genetic testing method with high efficiency. it is applicable for the diagnosis of AR-CMT. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Neurogenetic diseases are difficult to diagnose due to complicated phenotypes. Genetic testing is always the option for final diagnosis. With the progress and update of molecular diagnostic techniques, especially widely usage of next-generation sequencing (NGS), choosing proper sequencing methods is a new challenge. This paper aims to review different strategies and problems of genetic diagnosis for monogenic neurogenetic diseases based on clinical phenotypes, gene mutation characteristics and gene sequencing methodology. [ABSTRACT FROM AUTHOR]

The characteristics of inflorescences and spikelets of rice(Oryza sativa L.) help determine grain yield. The sterile lemmas are special organs of rice not found in other grasses. The genes regulating the development of sterile lemmas are also involved in regulating grain yield-associated traits. Understanding the molecular mechanisms of sterile lemma development is pivotal for elucidating the mechanisms of rice flower development. In this paper, we characterized 12 known genes that directly affect the development of sterile lemma. The expression patterns of the 12 genes were determined in sterile lemma and other rice flower organs. On the basis of the results and reasonable assumptions, we proposed a working model to better understand the molecular mechanisms of sterile lemma development in rice, and the research area worth further exploration were put forward. [ABSTRACT FROM AUTHOR]

The performance of micro-population teaching-learning-based optimization(TLBO) algorithm is mainly depended on the population diversity. In order to further improve its performance, this paper proposed a micro-population teaching-learningbased optimization based on gene level diversity(MTLBO-GLD) . In the proposed algorithm, the pop ulation diversity was monitored at the genetic level, and the chaos search and the cosine function were used to improve the population diversity in different evolutionary stages. The proposed algorithm was compared with eight meta-heuristic algorithms ( including four micro-population algorithms and four non-micro-population algorithms) on 13 test functions . The experimental results show that the overall performance of the MTLBO-GLD is significantly better than that of compared algorithms. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Central China Normal University is the property of Huazhong Normal University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[ Objectives J The paper aimed to identify the DnaJ protein family genes in cucumber and analyze its expression levels under high temperature stress. [ Methods] Gene family members, chromosome position, protein characteristics, structural characteristics, evolution and replication patterns, tissue expression characteristics were identified using hioinfonnatics software, the cucumber reference genome database, and public databases, while their expression patterns under high temperature stress environment were analyzed by quantitative real-time PCR. [ Results J A total of 81 CsDna] member genes of cucumber were identified. Chr 3 contained the largest number of Dna] genes ( 16), and Chr 4 and Chr 7 contained the minimum ( 9). The length of the proteins sequence encoded by the CsDna] genes varied from 113 to 2 551 aa, which contained at least 1 complete core region sequence of the ]-domain. The prediction of suhcellular location showed that most of the proteins were located in the nucleus or chloroplast. The structure of CsDna] genes showed that each gene contained 1 to 22 exons. Evolutionary analysis showed that CsDnaJ proteins could he distinguished into 9 suhclusters, each of which contained homologous proteins in Arabidopsis thaliana. Collinearity analysis showed that there were 17 collinear genes in the CsDna] gene family, fonning 10 pairs of duplicating gene pairs, including 5 pairs of fragment repeating events, 3 pairs of tandem repeating events,and 2 pairs of random repeating events. The expression levels of CsDna] genes in leaf, stem and ovary were slightly higher than those in male flower, female flower, root and tendrils. Quantitative real-time PCR analysis showed that the high temperature environment( the temperature of day/night was 42 °C/35 °C) could induce the high expression of CsDna] genes to respond to high temperature stress environmental. [ Conclusions] The core structure of the CsDna] gene family in cucumber is highly conserved and it plays an important role in response to high temperature stress in cucumber. [ABSTRACT FROM AUTHOR]