1. 间充质干细胞来源的外泌体抑制乳腺癌细胞生长的研究.
- Author
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何宁宁, 王津晗, 王彦, 杜利清, 徐畅, 冯宇, 孙晓辉, 蔡慧, and 刘强
- Abstract
Objective To study the effect of mesenchymal stem cell derived exosomes (MSC-Exo) on the proliferation of breast cancer cells. Methods The human breast cancer cell line MDA-MB-231 (231-Fluc-GFP) was constructed by lentiviral transfection using a double-reporter gene (fluorescein-green fluorescent protein, fluc-GFP) vector based on bioluminescence imaging technology, so that the cells were monitored in vitro in real time. Cells were treated with MSCs-Exo (MSCs-Exo group) and PBS (control group) respectively. The morphological observation, proliferation, survival rate and results of real-time PCR in vitro cells were performed in two groups of cells. In vivo, a nude mouse breast cancer model was established, and the tumor formation under different treatment conditions was analyzed. Results The proliferation of breast cancer MDA-MB-231 cells was inhibited after treatment with mesenchymal stem cell-derived exosomes. There were significant differences in cell numbers on the 3rd day of treatment between the two groups (P<0.05). The survival rate of breast cancer cells decreased after treatment (P<0.01). The expression of STAT3 and its downstream genes C-MYC,BCLXL, NANOG,OCT4 and SOX2 decreased in exosome treatment group. In vivo, the growth of tumor cells treated with MSCs- Exo was inhibited. Conclusion Mesenchymal stem cell-derived exosomes inhibit breast cancer cell growth [ABSTRACT FROM AUTHOR]
- Published
- 2019
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