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【Objective】Biological characteristics of Colletotrichum hymenocallidis were tested to provide theoretical basis for control of Dalbergia odorifera T.Chen anthracnose.【Method】Effects of temperatures gradient of 5-40 °C on the mycelium growth,sporulation and spore germination of C.hymenocallidis were studied on PDA medium.Effects of 50 %-100 % different humidity on the conidial germination ofC.hymenocallidis were studied by means of small container air humidity adjustment method.Effects of PDB culture solution with pH 2-12 on the mycelium growth of C.hymenocallidis was determined by liquid culture method.With Czapek media as the base,sucrose and sodium nitrate were replaced by nine kinds of carbon sources or nitrogen sources of the same mass,in order to studied the effects of different carbon sources and nitrogen sources on the mycelium growth,sporulation quantity of C.hymenocallidis.Effects of darkness,continuous illumination and 12 h light and dark alternation on the myceliuml growth,sporulation quantity were studied on PDA medium.【Result】The tested strain grew differently under different temperatures.Extremely high and low temperature were not beneficial to mycelium growth,sporulation and spore germination of C.hymenocallidis.All biological indicators could reach the highest level under 28 °C.The colony diameter,sporulation quantity and spore germination rate were 69.88 mm,3.33×10~6/mL and 94.00 %,respectively.The germination rate of the bacteria was the highest in the water droplets,the germination rate was 93.00 %.The strain could grow in the range of pH 3-12,and the optimum was 5.The different carbon and nitrogen source could be used,the better carbon sources was D-fructose,sucrose,D-xylose,mannitol,D-sorbitol and amylogen,but no significant difference on sporulation(P>0.05).The better nitrogen sources was yeast powder on the mycelium growth and the optimal nitrogen for spore germination was beef extract.The light can accelerate mycelium growth and promote sporulation.【Conclusion】The mycelial growth and sporulation of C.hymenocallidis were significantly affected by temperature,pH,carbon and nitrogen sources and light.The suitable high temperature and high humidity were conducive to the germination of conidia of C.hymenocallidis.The biological characteristics of C.hymenocallidis can be used for effective prevention and control in production. [ABSTRACT FROM AUTHOR]