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【Objective】 It is of great interest to understand the mechanism underlying breast milk quality for immune disease research. 【Method】 The expression changes of let-7a and let-7f miRNAs were analyzed in porcine milk exosomes across the entire lactation period (newborn to 28 days after birth) by quantitative real-time PCR. 【Result】 let-7a and let-7f were more abundant in the early lactation period (0 and 3 days) than in the later lactation period (7, 14, 21 and 28 days), and were expressed at higher levels in Meishan pigs compared with Yorkshire pigs throughout the entire lactation period. 【Conclusion】 This study is a preludetoin-depth investigations of the essential roles of breast milk exosome-packaged m iRNAs in the development of the infant's immune system. [ABSTRACT FROM AUTHOR]

The experiment was conducted to compare the thiamine content in longissimus dorsi muscle of Chuanzang black pigs and DLY pigs with different stages (90, 110 and 130 kg). The results showed that the thiamine content were 1. 77,2. 16 and 2. 83 μg/g in longissimus dorsi muscle of Chuanzang black pig at 90,110 and 130 kg, while the thiamine content were 1.29, 1.63 and 1.94 μg/g of DLY. We also found that the thiamine levels were significantly higher in Chuanzang black pigs than those in DLY pigs at 90, 110 and 130 kg (P90kg < 0.05, P130kg <0.05). Correlation analysis showed that thiamine levels were highly correlated with weight (P < 0. 01). These differences in levels of the thiamine may also have indications for meat quality. Our study revealed the deposition law of thiamine in longissimus dorsi muscle at different developmental stages of the two pig breeds. This study also provides data for further study on the pork flavor. [ABSTRACT FROM AUTHOR]

The intramuscular fat (IMF) in Longissimus dorsi muscle of the pigs at different stages (90,110 and 130 kg) were measured with automatic extraction and classical soxhlet methods. The results showed that the repeatability limit requirements were up to a given standardand performed a strong positive correlation (P <0. 01) between two methods. Moreover, it was also found that there was no significant difference of IMF in the Longissimus dorsi muscle between two methods (P >0. 05). These results indicate that the IMF was more thoroughly extracted and the fat rate was higher with the traditional extraction technology than that with the automatic extraction, while the automatic extraction exhibited more efficient, safety and environmental protection. [ABSTRACT FROM AUTHOR]