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Objective: To investigate the effects of catechin on myocardial injury and anti-inflammatory effects in rats with coronary heart disease through PI3K-Akt-eNOS signaling pathway. Methods: Healthy adult SPF-grade SD male rats were injected with pituitrin intraperitoneally to establish coronary heart disease model. The expression of PI3K-Akt-eNOS signaling pathway was detected by Western blot. The expressions of inflammatory factors and oxidative stress were detected by ELISA. Myocardial injury in rats was observed and recorded. Results: Compared with blank group, myocardial ischemia and myocardial infarction area in model group, catechin low-dose group, catechin medium-dose group and catechin high-dose group were higher (P<0.05). The area of myocardial ischemia and myocardial infarction in catechin high-dose group was smaller than that in model group, catechin low-dose group and catechin medium-dose group (P<0.05). Compared with blank group, PI3K, p-Akt /Akt and P-ENOS /eNOS in model group, catechin low-dose group, catechin medium-dose group and catechin high-dose group were higher (P<0.05). PI3K, P-Akt /Akt and P-ENOS /eNOS in catechin high-dose group were higher than those in model group, catechin low-dose group and catechin medium-dose group (P<0.05); Compared with blank group, TNF-α, IL-1βand IL-18 in model group, catechin low-dose group, catechin medium-dose group and catechin high-dose group were higher (P<0.05). TNF-α, IL-1βand IL-18 in catechin high-dose group were lower than those in model group, catechin low-dose group and catechin medium-dose group (P<0.05). Compared with blank group, ROS, GSH-Px and MDA in model group, catechin low-dose group, catechin medium-dose group and catechin high-dose group were higher (P<0.05). TNF-α, IL-1β and IL-18 in catechin high-dose group were lower than those in model group, catechin low-dose group and catechin medium-dose group (P<0.05). Conclusions: Catechin can improve myocardial inflammation and oxidative stress in rats with coronary heart disease by targeting the PI3K-Akt-eNOS signaling pathway, and thus play a myocardial protective role. [ABSTRACT FROM AUTHOR]