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To evaluate the application value of serum 25-hydroxyvitamin D [25 (OH)D], anti-Mullerian hormone (AMH) and inhibin B (INHB) in pregnancy outcome of in vitro fertilization-embryo transfer (IVR-ET) in patients with diminished ovarian reserve (DOR). 292 DOR patients who underwent IVR-ET assisted pregnancy in our hospital from August 2019 to October 2021 were selected, according to the pregnancy outcome after assisted pregnancy, they were divided into pregnancy group (n=158) and non-pregnancy group (n=134), the clinical data and the levels of serum 25(OH)D, AMH and INHB in the two groups were compared, the predictive value of serum 25 (OH)D, AMH and INHB in separate and combination detection for pregnancy failure was evaluated by the receiver operating characteristic (ROC) curve. The influencing factors of pregnancy failure were analyzed by single factor analysis and multiple logistic regression. The levels of serum 25 (OH)D, AMH and INHB in pregnant group were significantly higher than those in non-pregnancy group (P<0.05). ROC curve analysis result showed that the area under the curve (AUC) of serum 25(OH)D, AMH, INHB and their combined detection in predicting IVR-ET pregnancy failure in patients with DOR were 0.714 (0.631~0.802), 0.801 (0.731~0.852), 0.634 (0.578~0.741), 0.853 (0.806~0.913) respectively, the AUC of the combined detection in predicting IVR-ET pregnancy failure in patients with DOR was higher than those in the separate detection. Single factor analysis results showed that the number of sinus follicles<5, the number of retrieved eggs<8, follicle stimulating hormone (FSH) / luteinizing hormone (LH)≥ 1.8, serum 25 (OH)≤ 25.05 μg/L, serum AMH≤ 1.08 ng/mL, serum INHB≤ 84.28 pg/ml in the non-pregnancy group were significantly higher than those in pregnancy group (P<0.05). Multiple logistic regression analysis results showed that FSH/LH≥1.8, serum 25(OH)D≤ 25.05 μg/L, serum AMH≤ 1.08 ng/mL, serum INHB≤ 84.28 pg/mL were the risk factors of IVR-ET pregnancy failurein patients with DOR (P<0.05). The low levels of serum 25(OH)D, AMH and INHB are related to IVR-ET pregnancy failure in patients with DOR, and the combined detection of the above index levels has high auxiliary predictive value for IVR-ET pregnancy failure. [ABSTRACT FROM AUTHOR]

Objective: To study the relationship between serum leptin, irisin, sex hormone binding globulin (SHBG) and aquaporin 8 (AQP8) and sex hormone levels and pregnancy outcome in patients with polycystic ovary syndrome (PCOS) infertility undergoing in vitro fertilization-embryo transfer (IVF-ET). A total of 293 patients with PCOS infertility undergoing IVF-ET who were admitted to our Hospital from January 2020 to January 2022 were selected, and the patients were divided into clinical pregnancy failure group (n=118) and clinical pregnancy success group (n=175) according to whether or not the pregnancy was clinically successful. Clinical data of patients were collected, and serum leptin, irisin, SHBG and AQP8 levels were measured. Pearson's correlation coefficient was used to analyze the correlation between serum leptin, irisin, SHBG and AQP8 levels and sex hormone levels in PCOS infertility, multivariate Logistic regression was used to analyze influencing factors of pregnancy outcome in PCOS infertility patients undergoing IVF-ET, and receiver operating characteristic (ROC) curves were used to analyze the value of serum leptin, irisin, SHBG and AQP8 levels alone and in combination to predict clinical pregnancy failure in PCOS infertility patients undergoing IVF-ET. Compared to the clinical pregnancy success group, the clinical pregnancy failure group had lower follicle stimulating hormone (FSH), gonadotropin (Gn) dosage, number of eggs gained, fertilization rate, excellent embryo rate and SHBG levels, and higher luteinizing hormone (LH), LH/FSH, testosterone (T), insulin resistance index (HOMA-IR), leptin, irisin and AQP8 levels. Pearson correlation coefficients showed that serum leptin, irisin and AQP8 were negatively correlated with FSH level, and positively correlated with LH, LH/FSH and T levels in patients with PCOS infertility (all P＜0.05). SHBG was positively correlated with FSH level, and negatively correlated with LH, LH/FSHand T levels (all P＜0.05). Multivariate Logistic regression analysis showed that LH/FSH, T, HOMA-IR, leptin, irisin and AQP8 were independent risk factors for clinical pregnancy failure in patients undergoing IVF-ET for PCOS infertility, and excellent embryo rate and SHBG were independent protective factors (all P＜0.05). The ROC curves showed that the area under the curve (AUC) for serum leptin, irisin, SHBG and AQP8 levels in combination to predict clinical pregnancy failure in PCOS infertility patients undergoing IVF-ET were greater than each indicator alone. Serum leptin, irisin, SHBG and AQP8 levels are associated with FSH, LH, LH/FSH, T levels and clinical pregnancy failure in PCOS infertility patients undergoing IVF-ET, and it is helpful to predict the pregnancy outcome of patients with PCOS infertility. [ABSTRACT FROM AUTHOR]

Objective: To study the relationship between circulating tumor cells （CTC） and serum scc-ag and keratin 19 soluble fragment（cy-fra21-1） levels in patients with non-small cell lung cancer （NSCLC）. Methods: 100 NSCLC patients admitted to our hospital from February 2017 to February 2019 were included in the study, which was referred to as NSCLC group. Another 100 patients with benign lung lesions admitted to our hospital at the same time were taken as the benign lesion group. Another 100 healthy volunteers who underwent physical examination in our hospital at the same time were selected as the control group. The CTC and serum scc-ag and cy-fra21-1 levels of the three groups were detected and compared, and the correlation between CTC and serum scc-ag and cy-fra21-1 levels was analyzed by Pearson correlation. The diagnostic efficacy of CTC and serum scc-ag and cy-fra21-1 levels in NSCLC was analyzed by ROC curve. In addition, the relationship between CTCS, serum scc-ag, cy-fra21-1 and lymph node metastasis in NSCLC patients was analyzed. Results: The levels of CTC, serum scc-ag and cy-fra21-1 in the NSCLC group and the benign lesion group were higher than those in the control group, and the levels of CTC, serum scc-ag and cy-fra21-1 in the NSCLC group were higher than those in the benign lesion group （all P<0.05）. Pearson correlation analysis showed that there was a positive correlation between CTC and serum scc-ag and cy-fra21-1 levels in NSCLC patients （r=0.642、0.601, P<0.05）. According to ROC curve analysis, the combined detection of CTC, serum scc-ag and cy-fra21-1 levels in the diagnosis of NSCLC showed that the area under the curve, the sensitivity and the specificity were all higher than those measured separately （all P<0.05）. The levels of CTC and serum scc-ag and cy-fra21-1 in patients with NSCLC lymph node metastasis were higher than those in patients without lymph node metastasis （all P<0.05）. Conclusion: There is a significant positive correlation between CTCS and serum scc-ag and cy-fra21-1 levels in NSCLC patients, and the combined detection of the three can improve the diagnostic value of NSCLC, and the above three indicators are closely related to lymph node metastasis. [ABSTRACT FROM AUTHOR]

Objective: To explore the correlation of serum hydrogen sulfide (H2S), growth differentiation factor-15 (GDF-15), Pentraxin-3 (PTX-3) levels with blood coagulation function, inflammation indexes and disease score in sepsis patients. Methods: 52 cases of sepsis patients admitted to our hospital from February 2018 to February 2020 were randomly selected as the sepsis group, and 46 cases of septic shock patients admitted to the same period were selected as the shock group, at the same time, 50 healthy people who had physical examination in our hospital were taken as control group. Serum H2S, GDF-15, PTX-3 levels, coagulation function and inflammation indexes were detected in the three groups of subjects. The coagulation function indexes include platelet count (PLT), activited partial thomboplastin time (APTT), prothrombin time (PT), and fibrinogen (FIB). Acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ), Quick sequential organ failure assessment (qSOFA) were used to score the patients in the sepsis group and shock group. The correlation of serum H2S, GDF-15, PTX-3 with patients' coagulation function, inflammation indexes and disease score were analyzed. Results: The serum H2S, PLT in the sepsis group and shock group were lower than that in the control group, and the shock group was lower than the sepsis group (P<0.05). Serum GDF-15, PTX-3, APTT, PT, FIB, PCT, CRP in the sepsis group and shock group were higher than those in the control group, and the shock group was higher than the sepsis group (P<0.05). The scores of APACHEⅡ and qSOFA in the sepsis group were significantly lower than those in the shock group (P<0.05). Serum H2S was positively correlated with PLT (P<0.05), and negatively correlated with APTT, PT, FIB, PCT, CRP, APACHE II score, qSOFA score (P<0.05). Serum GDF-15, PTX-3 were negatively correlated with PLT (P<0.05), and positively correlated with APTT, PT, FIB, PCT, CRP, APACHE II score, qSOFA score (P<0.05). Conclusions: Serum H2S in sepsis patients was significantly down-regulated, while serum GDF-15 and PTX-3 are increased. The three are closely related to coagulation function, inflammation and disease score, which may be an important reason for the progress of sepsis. [ABSTRACT FROM AUTHOR]