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ASPS is a kind of plant polysaccharides extracted from the fruits, roots and stems of Acanthopanax senticosus. Its chemical components include galactose, glucose, arabinose, rhamnose, xylose, etc. It has physiological functions such as enhancing immunity, lowering blood glucose, anti-oxidation and regulating intestinal microecological balance. It has great potential as a new feed additive in livestock and poultry production. The paper reviews the biological functions of ASPS and its application in livestock and poultry production, in order to provide reference for further research and application of ASPS. [ABSTRACT FROM AUTHOR]

The study was to establish a method for the detection of Clostridium butyricum. In the test, the purified clostridium butyricum powder was diluted and coated in four different media, including modified FTG, FTG, TSC and TSN, to screen the appropriate medium, and the temperature and pH value gradient were set to screen the most appropriate conditions. Then, the detection method of Clostridium butyricum was verified by gram staining microscopy, biochemical identification, PCR and sequencing identification. The results showed that an improved FTG medium with low cost, strong specificity and easy to observe and count Clostridium butyricum was selected by observing morphology and colony count. The optimum culture conditions were pH (7.0±0.2) and temperature (37±1) ℃. The detected bacteria were grampositive, rod-shaped or spindle shaped, terminal or secondary spores, and positive for lactose, salicin, xylose, starch, melibiose and raffinose. Both the sample and the positive control of Clostridium butyricum showed a band at 548 bp, and the sequence homology was more than 96%, indicating that the detection result was accurate. The study indicates that an improved FTG medium with strong specificity and easy to observe and count Clostridium butyricum is screened, which can effectively detect the content of Clostridium butyricum. [ABSTRACT FROM AUTHOR]