1. [Over-expression of NUDT21 inhibits the proliferation of HCT-116 colon cancer cells via blocking P53/CDK2/Rb pathway].
- Author
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Luo Z, Dou L, Wang L, Liu R, Luo G, Lin M, Deng Z, Shen Y, Fu Z, Peng S, and Zhang Y
- Subjects
- Cell Cycle genetics, Cell Proliferation genetics, Cyclin-Dependent Kinase 2 genetics, Cyclin-Dependent Kinase 2 metabolism, HCT116 Cells, Humans, Retinoblastoma Protein genetics, Retinoblastoma Protein metabolism, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Cleavage And Polyadenylation Specificity Factor genetics, Cleavage And Polyadenylation Specificity Factor metabolism, Colonic Neoplasms genetics, Colonic Neoplasms metabolism
- Abstract
Objective To investigate the effect of over-expression of nudix hydrolase 21 (NUDT21) on the proliferation of colon cancer HCT-116 cells and its mechanism. Methods The NUDT21 over-expression plasmid was constructed by Gibson assembly. The colony formation assay and CCK-8 assay were used to detect cell proliferation. The cell cycle of HCT-116 cells was detected by flow cytometry. Western blot was performed to detect the expressions of P53, cyclin-dependent kinase 2 (CDK2), phosphorylated retinoblastoma protein at serine 780 (p-Rb-Ser780), and p-Rb-Ser608. Results The sequencing results showed that the NUDT21 over-expression plasmid was successfully constructed. After the NUDT21 over-expression plasmid was transfected into HCT-116 cells, the expressions of NUDT21 mRNA and protein in the cells were significantly increased. The over-expression of NUDT21 inhibited the proliferation of HCT-116 cells and arrested the cell cycle in G0/G1 phase. The expressions of CDK2, p-Rb-Ser608, and p-Rb-Ser780 proteins decreased while the expression of P53 protein increased. Conclusion Over-expression of NUDT21 inhibits the proliferation of HCT-116 cells by blocking P53/CDK2/Rb signal pathway.
- Published
- 2022